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The map label for this gene is leuA

Identifier: 75908332

GI number: 75908332

Start: 2611509

End: 2613104

Strand: Direct

Name: leuA

Synonym: Ava_2111

Alternate gene names: 75908332

Gene position: 2611509-2613104 (Clockwise)

Preceding gene: 75908331

Following gene: 75908333

Centisome position: 41.02

GC content: 44.67

Gene sequence:

>1596_bases
ATGACTACTAAACCAGAGAGAATTATCATTTTTGATACAACACTCCGTGACGGGGAACAGTGTCCTGGTGCAACACTGAA
TATAGATGAAAAGCTGGCGATCGCCAAACAGTTAGCCCGCTTAGGTGTAGATATCATAGAGGCAGGTTTCGCTTTTGCTA
GCCCTGGGGATTTTGAAGCAGTCCATAAAATTGCCCAAACTGTAGGGACGGAAAATGGCCCGGTAATTTGCAGTTTGGCA
AGAGCTAGGCATGATGATATCAAAGCGGCAGCCGAAGCTATTAAACCAGCAGCTAAAGGCAGGATTCACACCTTTATTGC
TACCTCAGATATTCACCTCCAGTACAAGCTGAAAAAAACCAGACCAGAAGTGATTGCGATCGCCGAAGAAATGGTAGCTT
ATGCCAAAAGTTTCACCGATGATGTGGAATTTTCCCCGGAAGATGCTGGACGTTCAGACCCAGAATTTTTGTATCAGGTT
TTAGAGCGAGCGATCGCCGCCGGTGCAACAACAATTAACATTCCGGATACCGTTGGTTACACCACACCAAGCGAATTTGG
GGCAATAATTAAGGGCATTAAAGAAAATGTCCCCAACATCGACCAAGCAATTATTTCTGTTCACGGACACAATGATTTAG
GCTTGGCAGTTGCCAACTTCTTAGAAGCAGTTAAAAATGGTGCAAGACAACTAGAATGCACCATCAATGGTATTGGTGAA
AGAGCAGGAAACGCCGCCTTAGAAGAATTGGTAATGGCGATGCACGTCCGCAGACAATACTTTAATCCCTTCTTAGGTAG
ACACCCAGATTCGGAAGAACCACTCACCAATATTGACACCAAGCAAATATATAAAACCTCACGCTTGGTTTCTAATTTAA
CAGGAATGTTAGTACAACCCAATAAGGCGATCGTTGGGGCTAACGCCTTCGCCCATGAATCAGGAATTCACCAAGATGGA
GTTTTGAAAAACAAACTCACCTACGAAATCATGGATGCCCAATTGATTGGCTTAACAGACAATCAAATAGTTTTAGGCAA
ACATTCTGGGCGTAATGCTTTCCGTACCCGCTTGAAAGAATTAGGCTTTGAACTATCAGAAACTGAGTTAAATAAAGCCT
TCGTCAAATTCAAAGAAGTCGCCGATAAAAAGAAAGAAATTTCTGATTGGGACTTGGAAGCAATTGTTAATGACGAAATC
CAGCAAGCCCCTGATTTATTCCGCGTAGAGTTGGTACAAGTTTCCTGCGGTAGCAATGCACGTCCAACAGCAACAGTTAC
CCTCCGCACCCCAGACGGCGAAGAACTCACCGACGCAGCCATTGGTACAGGGCCAGTAGACGCGGTATATAAAGCAATTA
ACCGCGTGGTCAACGTCCCCAACCAATTAATTGAGTTCTCCGTGCAGTCAGTAACAGCCGGGATTGATGCCATTGGGGAA
GTGACAATTCGTTTGCGGTATGAATCACGAGTATTTTCCGGTCATGCGGCCAACACCGATATCATCGTCGCCTCTGCACA
AGCCTACGTTAACGCCCTAAATCGTTTATACGCATCCTTGCAGACTCAAGATAAGCAAACAGAAGTAACAGCATAA

Upstream 100 bases:

>100_bases
AGAAGGTTAATATCCTTAGCAATTATTTACAAAATTAAAACTACAAAAACTTAGGTTCTAGATATTTTAAGCAAAACGTA
CAACTAAACAAATAGCCAAA

Downstream 100 bases:

>100_bases
AACTTAAAATAAACCCAACTCACTACAAGTTGGGTTTATTTATTTCTCCTAATTATTTACTTCAACGAATGAAGTTCTAT
AAATATCATGCTCTTGGTAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTTKPERIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEAVHKIAQTVGTENGPVICSLA
RARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKTRPEVIAIAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQV
LERAIAAGATTINIPDTVGYTTPSEFGAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMAMHVRRQYFNPFLGRHPDSEEPLTNIDTKQIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDG
VLKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKELGFELSETELNKAFVKFKEVADKKKEISDWDLEAIVNDEI
QQAPDLFRVELVQVSCGSNARPTATVTLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGE
VTIRLRYESRVFSGHAANTDIIVASAQAYVNALNRLYASLQTQDKQTEVTA

Sequences:

>Translated_531_residues
MTTKPERIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEAVHKIAQTVGTENGPVICSLA
RARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKTRPEVIAIAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQV
LERAIAAGATTINIPDTVGYTTPSEFGAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELVMAMHVRRQYFNPFLGRHPDSEEPLTNIDTKQIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDG
VLKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKELGFELSETELNKAFVKFKEVADKKKEISDWDLEAIVNDEI
QQAPDLFRVELVQVSCGSNARPTATVTLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGE
VTIRLRYESRVFSGHAANTDIIVASAQAYVNALNRLYASLQTQDKQTEVTA
>Mature_530_residues
TTKPERIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEAVHKIAQTVGTENGPVICSLAR
ARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKTRPEVIAIAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQVL
ERAIAAGATTINIPDTVGYTTPSEFGAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGER
AGNAALEELVMAMHVRRQYFNPFLGRHPDSEEPLTNIDTKQIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDGV
LKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKELGFELSETELNKAFVKFKEVADKKKEISDWDLEAIVNDEIQ
QAPDLFRVELVQVSCGSNARPTATVTLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGEV
TIRLRYESRVFSGHAANTDIIVASAQAYVNALNRLYASLQTQDKQTEVTA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=520, Percent_Identity=50, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6320071, Length=398, Percent_Identity=29.1457286432161, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=392, Percent_Identity=29.5918367346939, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=521, Percent_Identity=25.7197696737044, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324682, Length=329, Percent_Identity=27.355623100304, Blast_Score=106, Evalue=9e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_ANAVT (Q3MBA3)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322628.1
- HSSP:   P35914
- ProteinModelPortal:   Q3MBA3
- SMR:   Q3MBA3
- STRING:   Q3MBA3
- GeneID:   3680369
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2111
- NMPDR:   fig|240292.3.peg.1710
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q3MBA3
- ProtClustDB:   PRK00915
- BioCyc:   AVAR240292:AVA_2111-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57844; Mature: 57713

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKPERIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEA
CCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHH
VHKIAQTVGTENGPVICSLARARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKT
HHHHHHHHCCCCCCEEEEEHHHCHHHHHHHHHHHCHHHCCCEEEEEEEEEEEEEEEECCC
RPEVIAIAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQVLERAIAAGATTINIPDTVGY
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
TTPSEFGAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHHHCCCCHHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECHHH
RAGNAALEELVMAMHVRRQYFNPFLGRHPDSEEPLTNIDTKQIYKTSRLVSNLTGMLVQP
HCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEECC
NKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECEEEEECCCCEEEEECCCCCHHHHHHHHH
LGFELSETELNKAFVKFKEVADKKKEISDWDLEAIVNDEIQQAPDLFRVELVQVSCGSNA
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCC
RPTATVTLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGE
CCEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCE
VTIRLRYESRVFSGHAANTDIIVASAQAYVNALNRLYASLQTQDKQTEVTA
EEEEEEEECEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTKPERIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEA
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHH
VHKIAQTVGTENGPVICSLARARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKT
HHHHHHHHCCCCCCEEEEEHHHCHHHHHHHHHHHCHHHCCCEEEEEEEEEEEEEEEECCC
RPEVIAIAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQVLERAIAAGATTINIPDTVGY
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
TTPSEFGAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHHHHHHHHHHHHCCCCHHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECHHH
RAGNAALEELVMAMHVRRQYFNPFLGRHPDSEEPLTNIDTKQIYKTSRLVSNLTGMLVQP
HCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEEECC
NKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKE
CCEEEECCHHHHCCCCCCCCCHHCCCEEEEECEEEEECCCCEEEEECCCCCHHHHHHHHH
LGFELSETELNKAFVKFKEVADKKKEISDWDLEAIVNDEIQQAPDLFRVELVQVSCGSNA
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCC
RPTATVTLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGE
CCEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCE
VTIRLRYESRVFSGHAANTDIIVASAQAYVNALNRLYASLQTQDKQTEVTA
EEEEEEEECEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA