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The map label for this gene is azoB [H]

Identifier: 75908141

GI number: 75908141

Start: 2383580

End: 2384566

Strand: Direct

Name: azoB [H]

Synonym: Ava_1920

Alternate gene names: 75908141

Gene position: 2383580-2384566 (Clockwise)

Preceding gene: 75908140

Following gene: 75908142

Centisome position: 37.44

GC content: 44.07

Gene sequence:

>987_bases
ATGACATTATTAATAGTCGGTGCCACTGGCACCTTAGGAAGACAAGTGGCTCGTCGGGCGATCGATGAGGGATATAAAGT
GCGTTGTCTTGTCAGAAGCCCTAAAAGAGCAGCTTTTTTAAAAGAATGGGGAGCCGAACTCGTCAGAGGAGATTTATGTC
AACCCCAGACTCTGGCAGAGGCGCTAGAAGGCGTAACAGCAGTAATTGATGCAGCTACTTCTCGCGCTACAGATTCGCTG
ACAATCAAGCAGGTGGACTGGGAAGGACAAATAGCCCTGATCCAGGCAGCAAAGGCGGCGAGTGTAGAACGTTTTATATT
CTTCTCTATTATTGATGCCGATAAATATCCAGAAGTCCCACTGATGGAAATTAAACGGTGTACAGAATTATTTTTAGCTG
AATCTGGGATAAATTACACTGTTCTGCGCTTGGCTGGCTTCATGCAGGGGTTAATCGGTCAGTATGGCATCCCCATTTTA
GAAGGGCAACCTGTGTGGGTAACTGGCGCATCTTCCCCAGTCGCCTATATGGATACTTTAGACATTGCTAAATTTGCTGT
GCGGGCGTTGAGTGTGCCAGAAACAGAAAAACAAGCTTTTCCTGTAGTGGGTACTCGTGCCTGGAGTGCCGAAGAAATCA
TTAACTTATGTGAACGCTTATCTGGCAAAGAGGCTAAAGTTAGACGAATGCCCATCAACCTACTACGAGCTGTGCGCGGT
CTGGCTAAGTTCTTTCAGTGGGGATGGAATATAGCCGACAGACTGGCATTTACCGAAGTTTTGGCTAGTGGTAGACCGCT
AAATGCTTCAATGGACGAAGTTTACACAGTCTTTGGGTTAGAGAAAGAACAAAGTACTACTTTAGAAAGCTATTTACAAG
AGTATTTCAGCCGGATTCTGAAAAAGCTCAAACAGTTAGATTACGAGAAAACTAAAACTAAAAAGCAGAAAGACAAAAAA
ACTCCCTTTAAAAAAGTCAATAGTTAA

Upstream 100 bases:

>100_bases
TCAGTATCAATTCTGTTCCTTAAAAACCATTAATTTTTATTTACAGATATAATTCTGTTAAGATTCTTTTCATAAAAGTT
TAAAATTTGTTACAACCCTC

Downstream 100 bases:

>100_bases
TAGTCGCCAGTCAACAGTCAGTAATCAACAGTTTTTTACCATAGAGTATAGACTTTTGATGCCAAACTAATGACTGGTGA
TCCAAAATGTGTAACGATTA

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTLLIVGATGTLGRQVARRAIDEGYKVRCLVRSPKRAAFLKEWGAELVRGDLCQPQTLAEALEGVTAVIDAATSRATDSL
TIKQVDWEGQIALIQAAKAASVERFIFFSIIDADKYPEVPLMEIKRCTELFLAESGINYTVLRLAGFMQGLIGQYGIPIL
EGQPVWVTGASSPVAYMDTLDIAKFAVRALSVPETEKQAFPVVGTRAWSAEEIINLCERLSGKEAKVRRMPINLLRAVRG
LAKFFQWGWNIADRLAFTEVLASGRPLNASMDEVYTVFGLEKEQSTTLESYLQEYFSRILKKLKQLDYEKTKTKKQKDKK
TPFKKVNS

Sequences:

>Translated_328_residues
MTLLIVGATGTLGRQVARRAIDEGYKVRCLVRSPKRAAFLKEWGAELVRGDLCQPQTLAEALEGVTAVIDAATSRATDSL
TIKQVDWEGQIALIQAAKAASVERFIFFSIIDADKYPEVPLMEIKRCTELFLAESGINYTVLRLAGFMQGLIGQYGIPIL
EGQPVWVTGASSPVAYMDTLDIAKFAVRALSVPETEKQAFPVVGTRAWSAEEIINLCERLSGKEAKVRRMPINLLRAVRG
LAKFFQWGWNIADRLAFTEVLASGRPLNASMDEVYTVFGLEKEQSTTLESYLQEYFSRILKKLKQLDYEKTKTKKQKDKK
TPFKKVNS
>Mature_327_residues
TLLIVGATGTLGRQVARRAIDEGYKVRCLVRSPKRAAFLKEWGAELVRGDLCQPQTLAEALEGVTAVIDAATSRATDSLT
IKQVDWEGQIALIQAAKAASVERFIFFSIIDADKYPEVPLMEIKRCTELFLAESGINYTVLRLAGFMQGLIGQYGIPILE
GQPVWVTGASSPVAYMDTLDIAKFAVRALSVPETEKQAFPVVGTRAWSAEEIINLCERLSGKEAKVRRMPINLLRAVRGL
AKFFQWGWNIADRLAFTEVLASGRPLNASMDEVYTVFGLEKEQSTTLESYLQEYFSRILKKLKQLDYEKTKTKKQKDKKT
PFKKVNS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=301, Percent_Identity=27.2425249169435, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 36624; Mature: 36493

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLIVGATGTLGRQVARRAIDEGYKVRCLVRSPKRAAFLKEWGAELVRGDLCQPQTLAE
CEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHH
ALEGVTAVIDAATSRATDSLTIKQVDWEGQIALIQAAKAASVERFIFFSIIDADKYPEVP
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHEEEEECCCCCCCCC
LMEIKRCTELFLAESGINYTVLRLAGFMQGLIGQYGIPILEGQPVWVTGASSPVAYMDTL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCHHHHHH
DIAKFAVRALSVPETEKQAFPVVGTRAWSAEEIINLCERLSGKEAKVRRMPINLLRAVRG
HHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHH
LAKFFQWGWNIADRLAFTEVLASGRPLNASMDEVYTVFGLEKEQSTTLESYLQEYFSRIL
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KKLKQLDYEKTKTKKQKDKKTPFKKVNS
HHHHHCCHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TLLIVGATGTLGRQVARRAIDEGYKVRCLVRSPKRAAFLKEWGAELVRGDLCQPQTLAE
EEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHH
ALEGVTAVIDAATSRATDSLTIKQVDWEGQIALIQAAKAASVERFIFFSIIDADKYPEVP
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHEEEEECCCCCCCCC
LMEIKRCTELFLAESGINYTVLRLAGFMQGLIGQYGIPILEGQPVWVTGASSPVAYMDTL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCHHHHHH
DIAKFAVRALSVPETEKQAFPVVGTRAWSAEEIINLCERLSGKEAKVRRMPINLLRAVRG
HHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHH
LAKFFQWGWNIADRLAFTEVLASGRPLNASMDEVYTVFGLEKEQSTTLESYLQEYFSRIL
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KKLKQLDYEKTKTKKQKDKKTPFKKVNS
HHHHHCCHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]