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The map label for this gene is ydgK [H]

Identifier: 75676427

GI number: 75676427

Start: 2438008

End: 2439300

Strand: Direct

Name: ydgK [H]

Synonym: Nwi_2242

Alternate gene names: 75676427

Gene position: 2438008-2439300 (Clockwise)

Preceding gene: 75676426

Following gene: 75676428

Centisome position: 71.66

GC content: 64.19

Gene sequence:

>1293_bases
ATGGCTGCGTACCGATCCTTTCGAGACACATCGATGACCGACGCCGATATGGACGGATGGGTGTCGCGAGCCCATCAGCC
GAGAGGGTTCCCTGAATTCGTCGCCCTCGTCGCCTGCATCATGGCGCTGAACCCGATCGCGACCGACATGATGCTGCCGG
CGATGCCGGACGTCCGCGATGCCTACGCCATCGTTCACCCCAATCATGTCCAGGCCGTAATCTCGGTGTATCTGGCCGGC
TTCGGCGCGGGCCAGTTCATCATGGGTCCGCTGTCGGACCGGTTCGGCCGCCGCCCGATCCTGATCGGCGGAATGCTGCT
CTACAGCGCGGCTGGCCTGTTGTCGGTGGTCGCGTCCTCGTTTGAAACGATGCTGCTGGCGCGCGTGCTGCAGGGAATCG
GAAGTTCGGCCGCCCGCGTCATCGCCACCTCTATCGTGCGCGACTGCTACAGTGGCCGGCGCATGGCGAGCGTGATGTCG
CTTGCGATGATGATCTTCATCTCGGTGCCCGTGCTTGCGCCATCACTCGGACAGGCGATCCTGCTGTTCTCGGAATGGCG
CGGCATCTTCGTGGTGCTGACCGCCTACGGCCTGCTCGCTTTGCTGTGGTGCGCGCTCCGATTGCCAGAGACCCTGCCGC
CCGCGGGGCGCAAGCCGCTGACCGCCGCAAACGTCTTCGACGCATTCCGTCAGACCGTCACCAACCGGCAGACCATCGGC
TACGCGCTCGCGGCCGGCGGCGTGCAGAGCGTGCTGTTCGCCTATGTCTTTTGTTCGCAACAGATCTTCACCGGGATCTA
CGGCCTCGGCGACTATTTCCCGCTCGCCTTCGCCGCCATCGCCACCGGCCTCGCCGCCGCTGGTTTTCTGAACGCCCGCA
TCGTCGGCCGGTTCGGCATGCGGGTGATTTCGCACGGCGCGTTGACGGGATCGGTGGTGATTGCTGCGCTGACGTTGCTC
GCGGCGTGGCAGGGCTGGCTATCGTTTCCGGTTTACATGGTGTTTGCGATCGGCACGATGTTCGCGTTCGGGCTGATGTT
CTCAAACTTCACCGCGCTCGCGATGGAGCCGCAAGGCCATATCGCCGGAACCGCCTCATCCCTGTTCGGCTCGATCACGA
CATTGATAGGCAGCGGCGTCGGCGCCATGATCGGACAGGACTTCAACGGCACCATGCTGCGGTTCACAGCAAGCGCCTTC
CTTTGCACCATCGCCGCTTTGGTCGTCGTGATCGTTGTCGAGCGCGGACGGTTGTTCGTGCCCCACAACCCCGCGGTCCC
GCCGCCGCGTTAA

Upstream 100 bases:

>100_bases
AGAGTGGAAACCGGTTTTCGGATATGATCACGCTCAATCGCAAAGAGATAAAGCCGGAGTCTGATTCAACGCGGTTGAAT
CAGACTCCGGCGGCATCGAT

Downstream 100 bases:

>100_bases
CGCGAACCGATTTCCATCCCCAGGGTCAGGCTCGGGGACATGCCTTGCTCGAAAACACGATCGACGTAAGATGCTCGCCT
GCCTCAAAAGGATCCGCCAT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAAYRSFRDTSMTDADMDGWVSRAHQPRGFPEFVALVACIMALNPIATDMMLPAMPDVRDAYAIVHPNHVQAVISVYLAG
FGAGQFIMGPLSDRFGRRPILIGGMLLYSAAGLLSVVASSFETMLLARVLQGIGSSAARVIATSIVRDCYSGRRMASVMS
LAMMIFISVPVLAPSLGQAILLFSEWRGIFVVLTAYGLLALLWCALRLPETLPPAGRKPLTAANVFDAFRQTVTNRQTIG
YALAAGGVQSVLFAYVFCSQQIFTGIYGLGDYFPLAFAAIATGLAAAGFLNARIVGRFGMRVISHGALTGSVVIAALTLL
AAWQGWLSFPVYMVFAIGTMFAFGLMFSNFTALAMEPQGHIAGTASSLFGSITTLIGSGVGAMIGQDFNGTMLRFTASAF
LCTIAALVVVIVVERGRLFVPHNPAVPPPR

Sequences:

>Translated_430_residues
MAAYRSFRDTSMTDADMDGWVSRAHQPRGFPEFVALVACIMALNPIATDMMLPAMPDVRDAYAIVHPNHVQAVISVYLAG
FGAGQFIMGPLSDRFGRRPILIGGMLLYSAAGLLSVVASSFETMLLARVLQGIGSSAARVIATSIVRDCYSGRRMASVMS
LAMMIFISVPVLAPSLGQAILLFSEWRGIFVVLTAYGLLALLWCALRLPETLPPAGRKPLTAANVFDAFRQTVTNRQTIG
YALAAGGVQSVLFAYVFCSQQIFTGIYGLGDYFPLAFAAIATGLAAAGFLNARIVGRFGMRVISHGALTGSVVIAALTLL
AAWQGWLSFPVYMVFAIGTMFAFGLMFSNFTALAMEPQGHIAGTASSLFGSITTLIGSGVGAMIGQDFNGTMLRFTASAF
LCTIAALVVVIVVERGRLFVPHNPAVPPPR
>Mature_429_residues
AAYRSFRDTSMTDADMDGWVSRAHQPRGFPEFVALVACIMALNPIATDMMLPAMPDVRDAYAIVHPNHVQAVISVYLAGF
GAGQFIMGPLSDRFGRRPILIGGMLLYSAAGLLSVVASSFETMLLARVLQGIGSSAARVIATSIVRDCYSGRRMASVMSL
AMMIFISVPVLAPSLGQAILLFSEWRGIFVVLTAYGLLALLWCALRLPETLPPAGRKPLTAANVFDAFRQTVTNRQTIGY
ALAAGGVQSVLFAYVFCSQQIFTGIYGLGDYFPLAFAAIATGLAAAGFLNARIVGRFGMRVISHGALTGSVVIAALTLLA
AWQGWLSFPVYMVFAIGTMFAFGLMFSNFTALAMEPQGHIAGTASSLFGSITTLIGSGVGAMIGQDFNGTMLRFTASAFL
CTIAALVVVIVVERGRLFVPHNPAVPPPR

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=398, Percent_Identity=27.6381909547739, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1790794, Length=192, Percent_Identity=31.25, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI48994889, Length=237, Percent_Identity=29.957805907173, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082312, Length=356, Percent_Identity=28.6516853932584, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790146, Length=236, Percent_Identity=31.3559322033898, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787065, Length=339, Percent_Identity=26.8436578171091, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45725; Mature: 45593

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYRSFRDTSMTDADMDGWVSRAHQPRGFPEFVALVACIMALNPIATDMMLPAMPDVRD
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCHH
AYAIVHPNHVQAVISVYLAGFGAGQFIMGPLSDRFGRRPILIGGMLLYSAAGLLSVVASS
CEEEECCHHHHHHHHHHHHHCCCCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
FETMLLARVLQGIGSSAARVIATSIVRDCYSGRRMASVMSLAMMIFISVPVLAPSLGQAI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLFSEWRGIFVVLTAYGLLALLWCALRLPETLPPAGRKPLTAANVFDAFRQTVTNRQTIG
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
YALAAGGVQSVLFAYVFCSQQIFTGIYGLGDYFPLAFAAIATGLAAAGFLNARIVGRFGM
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVISHGALTGSVVIAALTLLAAWQGWLSFPVYMVFAIGTMFAFGLMFSNFTALAMEPQGH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
IAGTASSLFGSITTLIGSGVGAMIGQDFNGTMLRFTASAFLCTIAALVVVIVVERGRLFV
CCHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PHNPAVPPPR
CCCCCCCCCC
>Mature Secondary Structure 
AAYRSFRDTSMTDADMDGWVSRAHQPRGFPEFVALVACIMALNPIATDMMLPAMPDVRD
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCCCHH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CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]