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The map label for this gene is plsY

Identifier: 75676199

GI number: 75676199

Start: 2197441

End: 2198031

Strand: Direct

Name: plsY

Synonym: Nwi_2009

Alternate gene names: 75676199

Gene position: 2197441-2198031 (Clockwise)

Preceding gene: 75676198

Following gene: 75676200

Centisome position: 64.59

GC content: 62.77

Gene sequence:

>591_bases
ATGTTTCCTAACGGCCTGATCGCTTTCGCCTTCGGCTACCTGCTCGGCTCCATCCCGTTCGGTATGATCCTGACGCGGAT
CGCGGGCACACAGGATTTGCGCTCGATCGGTTCGGGCAATATCGGCGCGACCAACGTTCTTCGTACCGGACGCAGGGGGC
TCGCGGCGGCGACGCTTCTGGGCGATATGCTGAAAGGCACGGCCGCCGTCGTGATCGCATTGCTCCTCTCCGGACCGGGC
GCTGCGATGGCTGCGACGCTTGGGGCATTCCTCGGCCATCTGTTTCCGGTGTGGCTCAAGTTCCGCGGCGGCAAGGGCGT
TGCGACATATATCGGCGTGCTGCTCGGCCTGTTCTGGCCGGCGGCGCTGGCCTTTTGCGCGATCTGGCTGCTCGTCGCCT
TCACGACGCGCTACTCATCGCTGTCGGCGCTACTCGCCAGCCTCACAACGCCATTGCTGTTGTGGGGTTTCGGGCATCCA
CAATTCGCGTTGCTGTTTGCGGTGCTGACCGTGCTGTTATGGTTCAAGCACCGCGAAAACATCCGGCGCCTTTTTGCCGG
AAGCGAAGGCAGGATCGGCGCGAAAGCGTAA

Upstream 100 bases:

>100_bases
TCGAATGTTGATGCCCGCGACGGTGTGAATATCTGCCGTGGCGTGACCATGCCGATCTATGGTTTACATCGACGGCATTT
GGCATTCGGAGCCCTTTTCG

Downstream 100 bases:

>100_bases
AACGCGTCGGTATCCGGCTGAGGGGGCGGCGTGAACATCCGGGATCCAGACAGACGGCTGAGCGAAGCGCAGCGGACCGA
CTGGCTGCGGCTGATCCGGT

Product: putative glycerol-3-phosphate acyltransferase PlsY

Products: NA

Alternate protein names: Acyl-PO4 G3P acyltransferase; Acyl-phosphate--glycerol-3-phosphate acyltransferase; G3P acyltransferase; GPAT; Lysophosphatidic acid synthase; LPA synthase

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MFPNGLIAFAFGYLLGSIPFGMILTRIAGTQDLRSIGSGNIGATNVLRTGRRGLAAATLLGDMLKGTAAVVIALLLSGPG
AAMAATLGAFLGHLFPVWLKFRGGKGVATYIGVLLGLFWPAALAFCAIWLLVAFTTRYSSLSALLASLTTPLLLWGFGHP
QFALLFAVLTVLLWFKHRENIRRLFAGSEGRIGAKA

Sequences:

>Translated_196_residues
MFPNGLIAFAFGYLLGSIPFGMILTRIAGTQDLRSIGSGNIGATNVLRTGRRGLAAATLLGDMLKGTAAVVIALLLSGPG
AAMAATLGAFLGHLFPVWLKFRGGKGVATYIGVLLGLFWPAALAFCAIWLLVAFTTRYSSLSALLASLTTPLLLWGFGHP
QFALLFAVLTVLLWFKHRENIRRLFAGSEGRIGAKA
>Mature_196_residues
MFPNGLIAFAFGYLLGSIPFGMILTRIAGTQDLRSIGSGNIGATNVLRTGRRGLAAATLLGDMLKGTAAVVIALLLSGPG
AAMAATLGAFLGHLFPVWLKFRGGKGVATYIGVLLGLFWPAALAFCAIWLLVAFTTRYSSLSALLASLTTPLLLWGFGHP
QFALLFAVLTVLLWFKHRENIRRLFAGSEGRIGAKA

Specific function: Catalyzes the transfer of an acyl group from acyl- phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP

COG id: COG0344

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plsY family

Homologues:

Organism=Escherichia coli, GI1789439, Length=184, Percent_Identity=40.2173913043478, Blast_Score=120, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSY_NITWN (Q3SR23)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318620.1
- STRING:   Q3SR23
- GeneID:   3674197
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_2009
- NMPDR:   fig|323098.3.peg.1381
- eggNOG:   COG0344
- HOGENOM:   HBG734487
- OMA:   SRIGEKK
- PhylomeDB:   Q3SR23
- ProtClustDB:   PRK00220
- BioCyc:   NWIN323098:NWI_2009-MONOMER
- HAMAP:   MF_01043
- InterPro:   IPR003811
- TIGRFAMs:   TIGR00023

Pfam domain/function: PF02660 DUF205

EC number: NA

Molecular weight: Translated: 20708; Mature: 20708

Theoretical pI: Translated: 11.94; Mature: 11.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10c2e7c0)-; HASH(0x10b39240)-; HASH(0x10f5a054)-; HASH(0x1122fbe0)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPNGLIAFAFGYLLGSIPFGMILTRIAGTQDLRSIGSGNIGATNVLRTGRRGLAAATLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHH
GDMLKGTAAVVIALLLSGPGAAMAATLGAFLGHLFPVWLKFRGGKGVATYIGVLLGLFWP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AALAFCAIWLLVAFTTRYSSLSALLASLTTPLLLWGFGHPQFALLFAVLTVLLWFKHREN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IRRLFAGSEGRIGAKA
HHHHHCCCCCCCCCCH
>Mature Secondary Structure
MFPNGLIAFAFGYLLGSIPFGMILTRIAGTQDLRSIGSGNIGATNVLRTGRRGLAAATLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHH
GDMLKGTAAVVIALLLSGPGAAMAATLGAFLGHLFPVWLKFRGGKGVATYIGVLLGLFWP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AALAFCAIWLLVAFTTRYSSLSALLASLTTPLLLWGFGHPQFALLFAVLTVLLWFKHREN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IRRLFAGSEGRIGAKA
HHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA