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The map label for this gene is pyrB [H]

Identifier: 75676197

GI number: 75676197

Start: 2194720

End: 2195808

Strand: Direct

Name: pyrB [H]

Synonym: Nwi_2007

Alternate gene names: 75676197

Gene position: 2194720-2195808 (Clockwise)

Preceding gene: 75676194

Following gene: 75676198

Centisome position: 64.51

GC content: 63.82

Gene sequence:

>1089_bases
ATGCCCGAACAGCGGCCAGATCACGCCGGCGCTCTCGCCTTGGTTTACCGTGACCTCGCTTCGGCGGGGGAAAACCCGCC
TGCGCGCTGTTCGGCCTTGCCCCCACGGACGAGTGTGCCTATAGCTCTGGCTTTAATGACATCGTCCGCCAAATCGAGTT
TTGTCCTCGGACACCGGCATCTGCTGGGCATCGAGGGCCTTTCCGTCGCCGATATTACCGGCCTTCTCGATCTTTCCGAA
GAATACGTCGAACTGAACCGCCAGGTGGACAAGAAACGCACCTCCTTGCGCGGTCTCACCCAGGTCAACCTGTTCTTCGA
GGCTTCGACCCGCACCCAGTCGTCATTCGAGATCGCCGGCAAGCGGCTCGGCGCCGACGTTATGAATATGTCGGTGTCGT
CGCTATCGACCCGCAAGGGCGAAACGCTGGTCGATACCGCGATGACGCTCAACGCCATGCATCCGGACATCCTTGTGATG
CGCCATTCAGCCTCCGGCGCGGTGGAACTTCTGGCGCGCAAGGTCGACGGCTCCGTCGTCAATGCCGGCGACGGCGCCCA
CGAACATCCGACCCAGGCGCTGCTGGACGCATTGACCATCCGCCGCAACAAGGGACGGCTCGACGGACTTCTGGTCGCGA
TCTGCGGCGACGTTCTCCACTCGCGCGTCGCGCGCTCCAACATCATCCTGCTCAACGCCATGGGCGCGCGGGTCCGCGTC
GTCGCGCCGTCCACGCTGCTGCCGCCGGGCATCGAGCGCATGGGCGTCGAAGTCGCCCGCGATATGCGCGAGGGATTGGA
CGGCGCGGACATCGTGATGATGCTGCGGCTGCAGCGCGAGCGGATGAGCGGCTCGTTCGTGCCGTCGAGTTCCGAGTACT
TCCACTATTTCGGCCTCGACCAGAAAAAGCTCGCCTATGCCAAACCCAACGCGCTGGTCATGCATCCGGGACCGATGAAT
CGCGGCGTCGAGATTGACTCGATCGTCGCGGACGGCACGCAGTCGCTGATCCGCGAGCAGGTGGAAATGGGCGTGGCGGT
GCGGATGGCGGTGCTGGAAGCGCTGGCGAGGAACCTGCCGAATGCATGA

Upstream 100 bases:

>100_bases
GTCGACGTCCTCGAACGGCGGCGACTGATTGAGGACCTCGTGCGTCCTGAAGGGCTGCGTCATGATCCGCCTCCGCCGGC
GCTGATATCCATGTGACTTT

Downstream 100 bases:

>100_bases
ACCCGAGCCCTCCCACCGATTTGCACGGCGAGCATTGTCACGCGCGATGTTAGCGAACAGTTCGCAAAGGTGCGGCATAT
GCTGAGCGGCCATCGCCCGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MPEQRPDHAGALALVYRDLASAGENPPARCSALPPRTSVPIALALMTSSAKSSFVLGHRHLLGIEGLSVADITGLLDLSE
EYVELNRQVDKKRTSLRGLTQVNLFFEASTRTQSSFEIAGKRLGADVMNMSVSSLSTRKGETLVDTAMTLNAMHPDILVM
RHSASGAVELLARKVDGSVVNAGDGAHEHPTQALLDALTIRRNKGRLDGLLVAICGDVLHSRVARSNIILLNAMGARVRV
VAPSTLLPPGIERMGVEVARDMREGLDGADIVMMLRLQRERMSGSFVPSSSEYFHYFGLDQKKLAYAKPNALVMHPGPMN
RGVEIDSIVADGTQSLIREQVEMGVAVRMAVLEALARNLPNA

Sequences:

>Translated_362_residues
MPEQRPDHAGALALVYRDLASAGENPPARCSALPPRTSVPIALALMTSSAKSSFVLGHRHLLGIEGLSVADITGLLDLSE
EYVELNRQVDKKRTSLRGLTQVNLFFEASTRTQSSFEIAGKRLGADVMNMSVSSLSTRKGETLVDTAMTLNAMHPDILVM
RHSASGAVELLARKVDGSVVNAGDGAHEHPTQALLDALTIRRNKGRLDGLLVAICGDVLHSRVARSNIILLNAMGARVRV
VAPSTLLPPGIERMGVEVARDMREGLDGADIVMMLRLQRERMSGSFVPSSSEYFHYFGLDQKKLAYAKPNALVMHPGPMN
RGVEIDSIVADGTQSLIREQVEMGVAVRMAVLEALARNLPNA
>Mature_361_residues
PEQRPDHAGALALVYRDLASAGENPPARCSALPPRTSVPIALALMTSSAKSSFVLGHRHLLGIEGLSVADITGLLDLSEE
YVELNRQVDKKRTSLRGLTQVNLFFEASTRTQSSFEIAGKRLGADVMNMSVSSLSTRKGETLVDTAMTLNAMHPDILVMR
HSASGAVELLARKVDGSVVNAGDGAHEHPTQALLDALTIRRNKGRLDGLLVAICGDVLHSRVARSNIILLNAMGARVRVV
APSTLLPPGIERMGVEVARDMREGLDGADIVMMLRLQRERMSGSFVPSSSEYFHYFGLDQKKLAYAKPNALVMHPGPMNR
GVEIDSIVADGTQSLIREQVEMGVAVRMAVLEALARNLPNA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=341, Percent_Identity=35.4838709677419, Blast_Score=167, Evalue=9e-42,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=34.2948717948718, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790703, Length=233, Percent_Identity=28.3261802575107, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=351, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=335, Percent_Identity=34.3283582089552, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24642586, Length=320, Percent_Identity=33.125, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 39044; Mature: 38913

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQRPDHAGALALVYRDLASAGENPPARCSALPPRTSVPIALALMTSSAKSSFVLGHRH
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCHH
LLGIEGLSVADITGLLDLSEEYVELNRQVDKKRTSLRGLTQVNLFFEASTRTQSSFEIAG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHH
KRLGADVMNMSVSSLSTRKGETLVDTAMTLNAMHPDILVMRHSASGAVELLARKVDGSVV
HHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEE
NAGDGAHEHPTQALLDALTIRRNKGRLDGLLVAICGDVLHSRVARSNIILLNAMGARVRV
ECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEE
VAPSTLLPPGIERMGVEVARDMREGLDGADIVMMLRLQRERMSGSFVPSSSEYFHYFGLD
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
QKKLAYAKPNALVMHPGPMNRGVEIDSIVADGTQSLIREQVEMGVAVRMAVLEALARNLP
CHHEEEECCCEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NA
CC
>Mature Secondary Structure 
PEQRPDHAGALALVYRDLASAGENPPARCSALPPRTSVPIALALMTSSAKSSFVLGHRH
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECCHH
LLGIEGLSVADITGLLDLSEEYVELNRQVDKKRTSLRGLTQVNLFFEASTRTQSSFEIAG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHH
KRLGADVMNMSVSSLSTRKGETLVDTAMTLNAMHPDILVMRHSASGAVELLARKVDGSVV
HHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEE
NAGDGAHEHPTQALLDALTIRRNKGRLDGLLVAICGDVLHSRVARSNIILLNAMGARVRV
ECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEE
VAPSTLLPPGIERMGVEVARDMREGLDGADIVMMLRLQRERMSGSFVPSSSEYFHYFGLD
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
QKKLAYAKPNALVMHPGPMNRGVEIDSIVADGTQSLIREQVEMGVAVRMAVLEALARNLP
CHHEEEECCCEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA