| Definition | Nitrobacter winogradskyi Nb-255, complete genome. |
|---|---|
| Accession | NC_007406 |
| Length | 3,402,093 |
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The map label for this gene is slt [H]
Identifier: 75676121
GI number: 75676121
Start: 2114362
End: 2116569
Strand: Direct
Name: slt [H]
Synonym: Nwi_1930
Alternate gene names: 75676121
Gene position: 2114362-2116569 (Clockwise)
Preceding gene: 75676116
Following gene: 75676122
Centisome position: 62.15
GC content: 66.26
Gene sequence:
>2208_bases GTGACATTGCCTGCCCGCGCATCAAAGCTACGAACGGCGGCTTTAGGGGCGAGCCTGGTGGCGGCCGCGCTATGGGCCGG AAGCGCCCAGGCGGAAAAAACCGCCAAAGTTCCCGCGCCTAAGTCCCGCCCCTCCGGCAACGCCGTTCCCAAGCCCGCTC CCGGCCATGGCAAAACCGCCGCGCCTCGCAAGGACAAAGCCACCGCGGCTCCGATCATCCAGCCCGCCACCCGCAGACAC GCCACCCTTCCCGCGCCATCGGCTCGCATGCCCGTTCCGAAAGCGGCTATGGCTGCGACATCATCAACCCCGCGCGCCGA TCTCGCCGCGCTCGACACGATCATTGACCTGACCCGCAGGCAGCGATCGTCCGAGGCGACGCAGGTCCAGGCCGGCATGA CGGATCCTGTGGCGCGCAAGCTCGCCGAGTGGCTTATCCTGCGCAGCAACGACAACGGCGCGCCGACCGAGCGCTATCGC GCCTTCATCGACGCCAATCCGAGTTGGCCCTCACAGTCATTCCTGCGCCGTCGCGCGGAAGCTGCGCTGTGGGACGACCA TCGCGACGCCGCCGCGGTGCTGGCCTGGTTCGAAAACGAGCGGCCGCAATCGGCCAAGGGACGGTTCGCTCTGGCAAGCG CGCTGCTCGCGCGCGGAGACCGCGCCCGCGCCGAACAGTTGGTCCGAGAGGCCTGGCGGACCGATACGATGTCGGCGGCG GTCGAGAGGAAAGCGCTGGAGATGTTCGGGGCGCTGATCACGCCGAGCGACCACAAAGCGCGCATGGATTACATGCTTTA CGGCGAGGACCGTGATGCCGGAATGCGCGCGGCCCAGCGGCTGGGAAGATCGCACGAGGCCCTTGCCAAGGCGCGGATCG CAGCCGACCGCAGATCGTCCAGCCTGCGCGCGCTGCTCGAAAGAGTGCCGAGCGAGCTTCACCGCGATCCCGGCTATATG CTGGCCTACATCCAGCTCCTGCGTCGCGAAGAGAAGTTCGCGGAAGCCGCGCGGCTGATGCAGAGCGCGCCCCGCGATCC CAACCGGCTTTATAATGTCGATCAATGGTGGATCGAGCGGCGGCTGCTCGCGCGCAAGATGATCGACGTCGGACAATACC GCACCGCCTATCTCATCGCCCGCGACGCGGCACTCCCCGCGCGCGATATCTACAAGACCGAACAGGAGTTCACCGCCGGC TGGATCGCTCTTCGCTTTTTGAACGATCCCAACACGGCCGCCCGGCATTTTGCACGCATCGGAGTCGGCAGCGTCAATCC GACGGCGCTCGCGCGGGCTGGCTACTGGCAGGGCCGCGCGGCCGAAGCGGCAGGCCGGACCCAGGAGGCTCGTCGCGCCT ATGCCGCCGCCGCGGAGCAGTCCACCAGCTACTATGGCCAGCTCGCACGCGCCAAGCTCGGGCTGCCGCAAATCGAACTG CGCAACGTTCCAGGCAGCCGGTCACGCGGAATCGAGCGGCTCGAGATCGTACGCGCCACACAGCTACTCTACGCTCTCGG CAAAGGCGACATCGCGATTCCGATCCTTGCCGATATGGGCGCGAACGGCGACCCCGACGCCGTGTTGGGGCTTGGCGAAC TCGCCGCACGTCATGGCGACGCGAGGGGAATGCTGCTCGCCGGCAAGGCCGCGTTGAACCGCGGACTGCCATTCGACTTC TATGCCTATCCGGTCAGCGGCATTCCGCCATTCAGATCAATCGGACCTGATGTGGAACGCAGCATCATTTACGCGATCGC CCGACAGGAAAGCGCGTTCAATCCGTCGGTGGTGTCGCCGGCCAACGCCTACGGGCTGATGCAGGTGACGCCGGATGCCG GACGATACGTCTGCAAGAAATACGGCGCCAGTTTCGACCTTCGCCGCCTGAAGACCGAGCCCGCCTATAACGCCGCCCTC GGCGCGGCGGAACTCGGCGGACTGCTCGACGACTATCGCGGCTCCTATATCCTGACCTTCGCCGCCTACAATGCCGGGCG CGGCAGCGTCAGGAAATGGATCGCGCGTTACGGTGACCCGCGAGATCCGGGAGTCGATGCGGTGGATTGGGTCGAACTGA TCCCGTTCTCGGAAACCCGCAACTACGTGCAGCGCATCATGGAGAACCTGCAGGTCTACCGCGCCCGCTTCGGCGGCGGC TCGAAGCTGCAGATCGAGGCCGACCTGCATCGAGGCGCGATCCAGTAA
Upstream 100 bases:
>100_bases AGAGCCCGATGTCGGCGGTTTTAGTATTTTGTCCACACATTCAATGAAATAGTTATCAGATTTTAGGGGTCCGCTTAGTT CACTGATAAAGGGATTTGCC
Downstream 100 bases:
>100_bases CGTGGGAAGCGGCGGATAACGCCTCGCGCGGTCCGCACCTCAAAGCATGTTGGCTTCTGTTGCGTCAGAAGCGAAGCTTG AGATTCTTGTGCTGACGCGT
Product: lytic transglycosylase
Products: 1,6-Anhydrobond [C]
Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]
Number of amino acids: Translated: 735; Mature: 734
Protein sequence:
>735_residues MTLPARASKLRTAALGASLVAAALWAGSAQAEKTAKVPAPKSRPSGNAVPKPAPGHGKTAAPRKDKATAAPIIQPATRRH ATLPAPSARMPVPKAAMAATSSTPRADLAALDTIIDLTRRQRSSEATQVQAGMTDPVARKLAEWLILRSNDNGAPTERYR AFIDANPSWPSQSFLRRRAEAALWDDHRDAAAVLAWFENERPQSAKGRFALASALLARGDRARAEQLVREAWRTDTMSAA VERKALEMFGALITPSDHKARMDYMLYGEDRDAGMRAAQRLGRSHEALAKARIAADRRSSSLRALLERVPSELHRDPGYM LAYIQLLRREEKFAEAARLMQSAPRDPNRLYNVDQWWIERRLLARKMIDVGQYRTAYLIARDAALPARDIYKTEQEFTAG WIALRFLNDPNTAARHFARIGVGSVNPTALARAGYWQGRAAEAAGRTQEARRAYAAAAEQSTSYYGQLARAKLGLPQIEL RNVPGSRSRGIERLEIVRATQLLYALGKGDIAIPILADMGANGDPDAVLGLGELAARHGDARGMLLAGKAALNRGLPFDF YAYPVSGIPPFRSIGPDVERSIIYAIARQESAFNPSVVSPANAYGLMQVTPDAGRYVCKKYGASFDLRRLKTEPAYNAAL GAAELGGLLDDYRGSYILTFAAYNAGRGSVRKWIARYGDPRDPGVDAVDWVELIPFSETRNYVQRIMENLQVYRARFGGG SKLQIEADLHRGAIQ
Sequences:
>Translated_735_residues MTLPARASKLRTAALGASLVAAALWAGSAQAEKTAKVPAPKSRPSGNAVPKPAPGHGKTAAPRKDKATAAPIIQPATRRH ATLPAPSARMPVPKAAMAATSSTPRADLAALDTIIDLTRRQRSSEATQVQAGMTDPVARKLAEWLILRSNDNGAPTERYR AFIDANPSWPSQSFLRRRAEAALWDDHRDAAAVLAWFENERPQSAKGRFALASALLARGDRARAEQLVREAWRTDTMSAA VERKALEMFGALITPSDHKARMDYMLYGEDRDAGMRAAQRLGRSHEALAKARIAADRRSSSLRALLERVPSELHRDPGYM LAYIQLLRREEKFAEAARLMQSAPRDPNRLYNVDQWWIERRLLARKMIDVGQYRTAYLIARDAALPARDIYKTEQEFTAG WIALRFLNDPNTAARHFARIGVGSVNPTALARAGYWQGRAAEAAGRTQEARRAYAAAAEQSTSYYGQLARAKLGLPQIEL RNVPGSRSRGIERLEIVRATQLLYALGKGDIAIPILADMGANGDPDAVLGLGELAARHGDARGMLLAGKAALNRGLPFDF YAYPVSGIPPFRSIGPDVERSIIYAIARQESAFNPSVVSPANAYGLMQVTPDAGRYVCKKYGASFDLRRLKTEPAYNAAL GAAELGGLLDDYRGSYILTFAAYNAGRGSVRKWIARYGDPRDPGVDAVDWVELIPFSETRNYVQRIMENLQVYRARFGGG SKLQIEADLHRGAIQ >Mature_734_residues TLPARASKLRTAALGASLVAAALWAGSAQAEKTAKVPAPKSRPSGNAVPKPAPGHGKTAAPRKDKATAAPIIQPATRRHA TLPAPSARMPVPKAAMAATSSTPRADLAALDTIIDLTRRQRSSEATQVQAGMTDPVARKLAEWLILRSNDNGAPTERYRA FIDANPSWPSQSFLRRRAEAALWDDHRDAAAVLAWFENERPQSAKGRFALASALLARGDRARAEQLVREAWRTDTMSAAV ERKALEMFGALITPSDHKARMDYMLYGEDRDAGMRAAQRLGRSHEALAKARIAADRRSSSLRALLERVPSELHRDPGYML AYIQLLRREEKFAEAARLMQSAPRDPNRLYNVDQWWIERRLLARKMIDVGQYRTAYLIARDAALPARDIYKTEQEFTAGW IALRFLNDPNTAARHFARIGVGSVNPTALARAGYWQGRAAEAAGRTQEARRAYAAAAEQSTSYYGQLARAKLGLPQIELR NVPGSRSRGIERLEIVRATQLLYALGKGDIAIPILADMGANGDPDAVLGLGELAARHGDARGMLLAGKAALNRGLPFDFY AYPVSGIPPFRSIGPDVERSIIYAIARQESAFNPSVVSPANAYGLMQVTPDAGRYVCKKYGASFDLRRLKTEPAYNAALG AAELGGLLDDYRGSYILTFAAYNAGRGSVRKWIARYGDPRDPGVDAVDWVELIPFSETRNYVQRIMENLQVYRARFGGGS KLQIEADLHRGAIQ
Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]
COG id: COG0741
COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)
Gene ontology:
Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transglycosylase slt family [H]
Homologues:
Organism=Escherichia coli, GI87082441, Length=152, Percent_Identity=38.1578947368421, Blast_Score=90, Evalue=6e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016026 - InterPro: IPR008258 - InterPro: IPR012289 - InterPro: IPR008939 - InterPro: IPR000189 [H]
Pfam domain/function: PF01464 SLT [H]
EC number: 3.2.1.- [C]
Molecular weight: Translated: 80518; Mature: 80386
Theoretical pI: Translated: 10.52; Mature: 10.52
Prosite motif: PS00922 TRANSGLYCOSYLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTLPARASKLRTAALGASLVAAALWAGSAQAEKTAKVPAPKSRPSGNAVPKPAPGHGKTA CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCC APRKDKATAAPIIQPATRRHATLPAPSARMPVPKAAMAATSSTPRADLAALDTIIDLTRR CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH QRSSEATQVQAGMTDPVARKLAEWLILRSNDNGAPTERYRAFIDANPSWPSQSFLRRRAE HHCCHHHHHHCCCCCHHHHHHHHHEEEECCCCCCCHHHHEEEEECCCCCCHHHHHHHHHH AALWDDHRDAAAVLAWFENERPQSAKGRFALASALLARGDRARAEQLVREAWRTDTMSAA HHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH VERKALEMFGALITPSDHKARMDYMLYGEDRDAGMRAAQRLGRSHEALAKARIAADRRSS HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH SLRALLERVPSELHRDPGYMLAYIQLLRREEKFAEAARLMQSAPRDPNRLYNVDQWWIER HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHH RLLARKMIDVGQYRTAYLIARDAALPARDIYKTEQEFTAGWIALRFLNDPNTAARHFARI HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHH GVGSVNPTALARAGYWQGRAAEAAGRTQEARRAYAAAAEQSTSYYGQLARAKLGLPQIEL CCCCCCHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE RNVPGSRSRGIERLEIVRATQLLYALGKGDIAIPILADMGANGDPDAVLGLGELAARHGD ECCCCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCCCCHHHHHHHHHHHHCCC ARGMLLAGKAALNRGLPFDFYAYPVSGIPPFRSIGPDVERSIIYAIARQESAFNPSVVSP CCCEEEECHHHHHCCCCCCEEECCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC ANAYGLMQVTPDAGRYVCKKYGASFDLRRLKTEPAYNAALGAAELGGLLDDYRGSYILTF CCCCEEEEECCCHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE AAYNAGRGSVRKWIARYGDPRDPGVDAVDWVELIPFSETRNYVQRIMENLQVYRARFGGG EECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC SKLQIEADLHRGAIQ CEEEEEECCCCCCCC >Mature Secondary Structure TLPARASKLRTAALGASLVAAALWAGSAQAEKTAKVPAPKSRPSGNAVPKPAPGHGKTA CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCC APRKDKATAAPIIQPATRRHATLPAPSARMPVPKAAMAATSSTPRADLAALDTIIDLTRR CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH QRSSEATQVQAGMTDPVARKLAEWLILRSNDNGAPTERYRAFIDANPSWPSQSFLRRRAE HHCCHHHHHHCCCCCHHHHHHHHHEEEECCCCCCCHHHHEEEEECCCCCCHHHHHHHHHH AALWDDHRDAAAVLAWFENERPQSAKGRFALASALLARGDRARAEQLVREAWRTDTMSAA HHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH VERKALEMFGALITPSDHKARMDYMLYGEDRDAGMRAAQRLGRSHEALAKARIAADRRSS HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH SLRALLERVPSELHRDPGYMLAYIQLLRREEKFAEAARLMQSAPRDPNRLYNVDQWWIER HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHH RLLARKMIDVGQYRTAYLIARDAALPARDIYKTEQEFTAGWIALRFLNDPNTAARHFARI HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEEEEEEECCCCHHHHHHHHH GVGSVNPTALARAGYWQGRAAEAAGRTQEARRAYAAAAEQSTSYYGQLARAKLGLPQIEL CCCCCCHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE RNVPGSRSRGIERLEIVRATQLLYALGKGDIAIPILADMGANGDPDAVLGLGELAARHGD ECCCCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCCCCHHHHHHHHHHHHCCC ARGMLLAGKAALNRGLPFDFYAYPVSGIPPFRSIGPDVERSIIYAIARQESAFNPSVVSP CCCEEEECHHHHHCCCCCCEEECCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCC ANAYGLMQVTPDAGRYVCKKYGASFDLRRLKTEPAYNAALGAAELGGLLDDYRGSYILTF CCCCEEEEECCCHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE AAYNAGRGSVRKWIARYGDPRDPGVDAVDWVELIPFSETRNYVQRIMENLQVYRARFGGG EECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC SKLQIEADLHRGAIQ CEEEEEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]
Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]
General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]