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The map label for this gene is queF

Identifier: 75676019

GI number: 75676019

Start: 2001574

End: 2002050

Strand: Direct

Name: queF

Synonym: Nwi_1828

Alternate gene names: 75676019

Gene position: 2001574-2002050 (Clockwise)

Preceding gene: 75676017

Following gene: 75676023

Centisome position: 58.83

GC content: 61.84

Gene sequence:

>477_bases
ATGCCCAGAAAGCCCCCCTCTTCGCCGAAATCCTCGCCTTTGCAGCTTGGCCGCGCCGTGGCGTGGCCTGATTCTCCGGA
GCAGGCAAAACTCGACCGCGTGCCCAATCCACAGAAGGGCACCAACTACGTCGCGCGGTTCACGGCGCCGGAATTCACCG
CGCTGTGCCCGGTCACCGGCCAGCCGGATTTTGCCCATTTGGTCATCGACTATGTGCCGGGGTCCTGGCTGCTGGAATCA
AAATCGCTCAAGCTGTATCTCGCCAGTTTTCGCAATCACGGGGCCTTCCACGAGGATTGCACTGTGGCGATCGGCAAGCG
CATCGTCGCCGCGATCAAACCGAAATGGCTGCGTATCGGCGGCTACTGGTTTCCGCGCGGCGGTATTCCCATTGATGTGT
TCTGGCAGACCGGAGCAGCGCCCAGGGGTGTCTGGATTCCCGATCAGGATGTGCCATCTTACCGAGGCCGGGGCTGA

Upstream 100 bases:

>100_bases
CGAGAGCCTTTCCGCTTCCGACCAAATCAAAAGCGGGGCTTCATGATTCTGATCTAAAATCGGCTTCCACTTGGCCGACC
CCGCTTGGACAGAGACGATC

Downstream 100 bases:

>100_bases
AAGGCGCGCGCCACCCGCGCATGCCGCTGCCGTCAGAAACCGATCGTTAGCGAAGCACTGAGACTCGCGCGCCAGACATC
GTCGATCCTGTAGGGATTGA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MPRKPPSSPKSSPLQLGRAVAWPDSPEQAKLDRVPNPQKGTNYVARFTAPEFTALCPVTGQPDFAHLVIDYVPGSWLLES
KSLKLYLASFRNHGAFHEDCTVAIGKRIVAAIKPKWLRIGGYWFPRGGIPIDVFWQTGAAPRGVWIPDQDVPSYRGRG

Sequences:

>Translated_158_residues
MPRKPPSSPKSSPLQLGRAVAWPDSPEQAKLDRVPNPQKGTNYVARFTAPEFTALCPVTGQPDFAHLVIDYVPGSWLLES
KSLKLYLASFRNHGAFHEDCTVAIGKRIVAAIKPKWLRIGGYWFPRGGIPIDVFWQTGAAPRGVWIPDQDVPSYRGRG
>Mature_157_residues
PRKPPSSPKSSPLQLGRAVAWPDSPEQAKLDRVPNPQKGTNYVARFTAPEFTALCPVTGQPDFAHLVIDYVPGSWLLESK
SLKLYLASFRNHGAFHEDCTVAIGKRIVAAIKPKWLRIGGYWFPRGGIPIDVFWQTGAAPRGVWIPDQDVPSYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_NITWN (Q3SRK3)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318440.1
- ProteinModelPortal:   Q3SRK3
- SMR:   Q3SRK3
- STRING:   Q3SRK3
- GeneID:   3675870
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1828
- NMPDR:   fig|323098.3.peg.1882
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   HEDCTVA
- ProtClustDB:   PRK13258
- BioCyc:   NWIN323098:NWI_1828-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 17507; Mature: 17376

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKPPSSPKSSPLQLGRAVAWPDSPEQAKLDRVPNPQKGTNYVARFTAPEFTALCPVTG
CCCCCCCCCCCCHHHHCEEEECCCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEECCCCC
QPDFAHLVIDYVPGSWLLESKSLKLYLASFRNHGAFHEDCTVAIGKRIVAAIKPKWLRIG
CCCEEEEEEEECCCCEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
GYWFPRGGIPIDVFWQTGAAPRGVWIPDQDVPSYRGRG
CEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRKPPSSPKSSPLQLGRAVAWPDSPEQAKLDRVPNPQKGTNYVARFTAPEFTALCPVTG
CCCCCCCCCCCHHHHCEEEECCCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEECCCCC
QPDFAHLVIDYVPGSWLLESKSLKLYLASFRNHGAFHEDCTVAIGKRIVAAIKPKWLRIG
CCCEEEEEEEECCCCEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
GYWFPRGGIPIDVFWQTGAAPRGVWIPDQDVPSYRGRG
CEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA