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The map label for this gene is pleD [H]

Identifier: 75675850

GI number: 75675850

Start: 1804565

End: 1805629

Strand: Direct

Name: pleD [H]

Synonym: Nwi_1658

Alternate gene names: 75675850

Gene position: 1804565-1805629 (Clockwise)

Preceding gene: 75675849

Following gene: 75675858

Centisome position: 53.04

GC content: 60.0

Gene sequence:

>1065_bases
GTGGTTGAGCTGAACGAACATGAACGCACCATGGCGTTCGCCGAGGTGGCGCTCGGCCAGATCCGTTCGCTGCGTCAGGC
CGCGGTGCCGCGCAATTATGAAGTCTGGTACGTCTACGCCGCCGGATATAACTGCTCCCTCAACAGGACCATCAACGAGA
CGCTGGCGCGCAACGGAACGCTGAGCGACGTTGATCTCGAGCAGATCTACGATACCTATCTTTCTCCGATCAGGACGACC
GAACGCATCGACAAGGTTGGCGCGCGGGTGATCAACGAAATCGACGAGGTGATACTTCTTATTACCGGCGCGCGCAGCAT
GGCGGCCGGTTTTGGAGACAGCTTGAGAGAAGCCGCCAGGCAGCTTGGCGCCGCCGGCAGTCATGCGCAGATCAAGTCGG
TCATCGCGGCGTTGATGACATCAACCCTCGACATGCAGGAAGCCAACAAGGTTCTGGAGGACCGGCTGGAGCTCTCGAAG
GTCGAGATCAATAACCTTCAGCATAGTCTTGAGGCCATCAGAGCTGAAAGTATGACGGATCCGCTCACCGGTCTCGGCAA
CCGAAAGTATTTCGATCGAGCCGTGGCCGACGCCGTGGCGCTGGCAATGAGCGAAGGCGAGCCGCTGTCGCTTCTGATGT
TCGACATCGACCATTTCAAGTCGTTCAACGACAACTACGGACACCTGACCGGCGACCAAGTCCTGCGGCTGGTCGCGATG
TCATTGAAGCAGAACATCAAGGGGCAGGACATCACCGCTCGTTACGGCGGTGAAGAATTCGCGGTCGTGCTGCCGAGCAC
CGCGCTGAAACAGGCGCTGACGGTCGCGGATCATATCCGGCGCGCCGTGATGGCCAAGGAGCTGAAGAAAAAATCGACCG
GCGAGATCCTGGGCCGCGTCACCATCTCGATCGGCGTCTCGATGCTTCGCCCCGGCGATACTCCGGACTCACTGATGGAG
CGCGCCGATGCCTGCCTGTACGCAGCCAAGCGTGGCGGCCGCAACCGCGCTATTTGCGAGACCGATCCGGAATACTTCGC
TGGCGCTGAGACTCGCGTGGCATGA

Upstream 100 bases:

>100_bases
TACCGCGCGTAAACTCCCTGTTGCTAACTTCAATCGATATTTACGTTGATATTTGCCGTTTAGGACGACGATCACGTGAA
TCGTCGGCAGGACCGCATCA

Downstream 100 bases:

>100_bases
GGCTCTGCCCTCGCCCATGAACGGCGTCGCTTTCGCTCGCCTATGGTCTTCCGGCCATCTACGTCCTCGTTCCGGCCACG
TCCTCAGGATGCCGTGACAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MVELNEHERTMAFAEVALGQIRSLRQAAVPRNYEVWYVYAAGYNCSLNRTINETLARNGTLSDVDLEQIYDTYLSPIRTT
ERIDKVGARVINEIDEVILLITGARSMAAGFGDSLREAARQLGAAGSHAQIKSVIAALMTSTLDMQEANKVLEDRLELSK
VEINNLQHSLEAIRAESMTDPLTGLGNRKYFDRAVADAVALAMSEGEPLSLLMFDIDHFKSFNDNYGHLTGDQVLRLVAM
SLKQNIKGQDITARYGGEEFAVVLPSTALKQALTVADHIRRAVMAKELKKKSTGEILGRVTISIGVSMLRPGDTPDSLME
RADACLYAAKRGGRNRAICETDPEYFAGAETRVA

Sequences:

>Translated_354_residues
MVELNEHERTMAFAEVALGQIRSLRQAAVPRNYEVWYVYAAGYNCSLNRTINETLARNGTLSDVDLEQIYDTYLSPIRTT
ERIDKVGARVINEIDEVILLITGARSMAAGFGDSLREAARQLGAAGSHAQIKSVIAALMTSTLDMQEANKVLEDRLELSK
VEINNLQHSLEAIRAESMTDPLTGLGNRKYFDRAVADAVALAMSEGEPLSLLMFDIDHFKSFNDNYGHLTGDQVLRLVAM
SLKQNIKGQDITARYGGEEFAVVLPSTALKQALTVADHIRRAVMAKELKKKSTGEILGRVTISIGVSMLRPGDTPDSLME
RADACLYAAKRGGRNRAICETDPEYFAGAETRVA
>Mature_354_residues
MVELNEHERTMAFAEVALGQIRSLRQAAVPRNYEVWYVYAAGYNCSLNRTINETLARNGTLSDVDLEQIYDTYLSPIRTT
ERIDKVGARVINEIDEVILLITGARSMAAGFGDSLREAARQLGAAGSHAQIKSVIAALMTSTLDMQEANKVLEDRLELSK
VEINNLQHSLEAIRAESMTDPLTGLGNRKYFDRAVADAVALAMSEGEPLSLLMFDIDHFKSFNDNYGHLTGDQVLRLVAM
SLKQNIKGQDITARYGGEEFAVVLPSTALKQALTVADHIRRAVMAKELKKKSTGEILGRVTISIGVSMLRPGDTPDSLME
RADACLYAAKRGGRNRAICETDPEYFAGAETRVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=38.9221556886228, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=288, Percent_Identity=30.2083333333333, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=36.5591397849462, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=176, Percent_Identity=36.9318181818182, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787262, Length=180, Percent_Identity=35.5555555555556, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786584, Length=204, Percent_Identity=29.9019607843137, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=35.3658536585366, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787541, Length=190, Percent_Identity=31.0526315789474, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787056, Length=188, Percent_Identity=33.5106382978723, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=191, Percent_Identity=29.8429319371728, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=25.1497005988024, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=31.2849162011173, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38917; Mature: 38917

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELNEHERTMAFAEVALGQIRSLRQAAVPRNYEVWYVYAAGYNCSLNRTINETLARNGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCC
LSDVDLEQIYDTYLSPIRTTERIDKVGARVINEIDEVILLITGARSMAAGFGDSLREAAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
QLGAAGSHAQIKSVIAALMTSTLDMQEANKVLEDRLELSKVEINNLQHSLEAIRAESMTD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLTGLGNRKYFDRAVADAVALAMSEGEPLSLLMFDIDHFKSFNDNYGHLTGDQVLRLVAM
CHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHH
SLKQNIKGQDITARYGGEEFAVVLPSTALKQALTVADHIRRAVMAKELKKKSTGEILGRV
HHHHCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TISIGVSMLRPGDTPDSLMERADACLYAAKRGGRNRAICETDPEYFAGAETRVA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MVELNEHERTMAFAEVALGQIRSLRQAAVPRNYEVWYVYAAGYNCSLNRTINETLARNGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCC
LSDVDLEQIYDTYLSPIRTTERIDKVGARVINEIDEVILLITGARSMAAGFGDSLREAAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
QLGAAGSHAQIKSVIAALMTSTLDMQEANKVLEDRLELSKVEINNLQHSLEAIRAESMTD
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLTGLGNRKYFDRAVADAVALAMSEGEPLSLLMFDIDHFKSFNDNYGHLTGDQVLRLVAM
CHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCCCCCCCCCCHHHHHHHHHH
SLKQNIKGQDITARYGGEEFAVVLPSTALKQALTVADHIRRAVMAKELKKKSTGEILGRV
HHHHCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TISIGVSMLRPGDTPDSLMERADACLYAAKRGGRNRAICETDPEYFAGAETRVA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA