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The map label for this gene is purN [H]

Identifier: 75675790

GI number: 75675790

Start: 1747145

End: 1747798

Strand: Direct

Name: purN [H]

Synonym: Nwi_1598

Alternate gene names: 75675790

Gene position: 1747145-1747798 (Clockwise)

Preceding gene: 75675789

Following gene: 75675795

Centisome position: 51.36

GC content: 64.37

Gene sequence:

>654_bases
ATGAAACGCCGTGTCGCCATCCTGATCTCCGGACGCGGATCGAACATGACGGCGCTGGTGGAGGCTGCAAAGGCCGAGGA
CTTTCCGGCTGAAATAGCCGTCGTCATTTCCAATAAACCCGGCGCGGCGGGCCTCGCCAGAGCCCAGGCGGCCGGCGTCG
AGACGCTCGTCATTGAAAGCAAGCCATTTGGCAAGGACCGTGCGGCGTTCGAGGCCGAACTGCAATCCGCTCTGGATGAC
AGGCGCATCGAATTCATCTGCCTCGGCGGCTTCATGCGGCTGTTCACAGCCGAGTTCGTCCGAGGCTGGCATGGACGGAT
GCTCAACATCCACCCCTCGCTGCTTCCATCATTCCGGGGCCTCGACCCCCACGGTCAGGCCCTGCGCGCGGGCGTGAAGA
TCTCAGGCGCCACGGTGCATTTCGTCGTCGCGGAAACAGATGCCGGCCCCATTGTCATGCAGGGCGCAGTGGCTGTGCGT
GACGACGACACGGCGGAGACGCTGGCCGCGCGCGTCCTGGACATCGAGCATCGCATCTACCCCGATGCGCTGCGGCTGGT
CGCGGGCGGCGGCACGCGACTGGATGGCGACATTTGCAAGACGTCGGCAAACGCCAGTCCGGACGATAGCCTGATCTCGC
CGTCCGTAATCTGA

Upstream 100 bases:

>100_bases
GTTGCTGGGCGATGTGATCGTTGCAGGCGATGAAGGCCGGGTCGTTTACGACGGTCAACTCGATCTCGCCGTATAGAACG
CCCCAGCAGGGAATGTGAGC

Downstream 100 bases:

>100_bases
CCACGATTGATCTGACCACGATTGATCTGACCGCGTCCTGCTATAGCGTTTTCGAGCGAAGCGGATGACGTGACGGTTCG
CGTGAAAAACGCGTCAGATC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKRRVAILISGRGSNMTALVEAAKAEDFPAEIAVVISNKPGAAGLARAQAAGVETLVIESKPFGKDRAAFEAELQSALDD
RRIEFICLGGFMRLFTAEFVRGWHGRMLNIHPSLLPSFRGLDPHGQALRAGVKISGATVHFVVAETDAGPIVMQGAVAVR
DDDTAETLAARVLDIEHRIYPDALRLVAGGGTRLDGDICKTSANASPDDSLISPSVI

Sequences:

>Translated_217_residues
MKRRVAILISGRGSNMTALVEAAKAEDFPAEIAVVISNKPGAAGLARAQAAGVETLVIESKPFGKDRAAFEAELQSALDD
RRIEFICLGGFMRLFTAEFVRGWHGRMLNIHPSLLPSFRGLDPHGQALRAGVKISGATVHFVVAETDAGPIVMQGAVAVR
DDDTAETLAARVLDIEHRIYPDALRLVAGGGTRLDGDICKTSANASPDDSLISPSVI
>Mature_217_residues
MKRRVAILISGRGSNMTALVEAAKAEDFPAEIAVVISNKPGAAGLARAQAAGVETLVIESKPFGKDRAAFEAELQSALDD
RRIEFICLGGFMRLFTAEFVRGWHGRMLNIHPSLLPSFRGLDPHGQALRAGVKISGATVHFVVAETDAGPIVMQGAVAVR
DDDTAETLAARVLDIEHRIYPDALRLVAGGGTRLDGDICKTSANASPDDSLISPSVI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=200, Percent_Identity=49, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI209869995, Length=200, Percent_Identity=49, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI209869993, Length=200, Percent_Identity=49, Blast_Score=197, Evalue=8e-51,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=39.7905759162304, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787483, Length=186, Percent_Identity=32.7956989247312, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=43.2432432432432, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320616, Length=201, Percent_Identity=30.3482587064677, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=47.6439790575916, Blast_Score=175, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22999; Mature: 22999

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRVAILISGRGSNMTALVEAAKAEDFPAEIAVVISNKPGAAGLARAQAAGVETLVIES
CCCEEEEEEECCCCCEEEEEEHHCCCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEC
KPFGKDRAAFEAELQSALDDRRIEFICLGGFMRLFTAEFVRGWHGRMLNIHPSLLPSFRG
CCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCC
LDPHGQALRAGVKISGATVHFVVAETDAGPIVMQGAVAVRDDDTAETLAARVLDIEHRIY
CCCCCHHHHCCEEECCCEEEEEEEECCCCCEEEECEEEEECCCHHHHHHHHHHHHHHHCC
PDALRLVAGGGTRLDGDICKTSANASPDDSLISPSVI
CCHHHEEECCCCEECCHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRRVAILISGRGSNMTALVEAAKAEDFPAEIAVVISNKPGAAGLARAQAAGVETLVIES
CCCEEEEEEECCCCCEEEEEEHHCCCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEC
KPFGKDRAAFEAELQSALDDRRIEFICLGGFMRLFTAEFVRGWHGRMLNIHPSLLPSFRG
CCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCC
LDPHGQALRAGVKISGATVHFVVAETDAGPIVMQGAVAVRDDDTAETLAARVLDIEHRIY
CCCCCHHHHCCEEECCCEEEEEEEECCCCCEEEECEEEEECCCHHHHHHHHHHHHHHHCC
PDALRLVAGGGTRLDGDICKTSANASPDDSLISPSVI
CCHHHEEECCCCEECCHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]