Click here to switch to the map view.

The map label for this gene is purM

Identifier: 75675789

GI number: 75675789

Start: 1746047

End: 1747120

Strand: Direct

Name: purM

Synonym: Nwi_1597

Alternate gene names: 75675789

Gene position: 1746047-1747120 (Clockwise)

Preceding gene: 75675784

Following gene: 75675790

Centisome position: 51.32

GC content: 64.62

Gene sequence:

>1074_bases
ATGACCGACAGGAACATCAGCCTGACCTATGCCGGGGCGGGCGTCGATATCGACGCCGGCAACCGGCTGGTCGACCTCAT
CAAGCCGATGGTGCGCGCAACCGCTCGCGCCGGCGCGGAGGCCGAGATCGGCGGATTCGGCGGCCTGTTTGATCTCAAGG
CCGCCGGATTCGCGGACCCGGTTCTGGTTGCGGCCAACGACGGCGTCGGAACCAAGGTCAAGATCGCGATCGAAACCGGC
ATCCACGATACCATCGGCATCGATCTTGTCGCCATGTCCGTCAACGATCTCGTCGTACAGGGCGCGGAGCCCTTGTTCTT
CCTTGATTATTTTGCCTGCGGCAAGCTTGATCCCGAAACCGCCGCGGCGATCGTGGCCGGAATCGCGGAAGGCTGCCGGG
AAGCGGGTTGCGCCCTGATCGGCGGCGAAACGGCTGAAATGCCCGGCCTCTACAAGGACGGTGACTACGACCTTGCCGGC
TTCGCGGTCGGCGCGGCGGAACGCGGCTCCCTGTTGCCCGGCCGCGATATCGCCGCCGGTGATGCGGTGATCGGACTCGC
GTCGTCCGGCGTGCATTCCAATGGCTATTCACTGGTCCGCAATATCGTCGAAGCCTCGGGTATCGCCCTCTCCGCTCCAG
CGCCATTCGCTCCCGTCGCGACCCTGGGAGGTGCGCTGCTGACCCCCACCCGGCTCTACGTCAAATCCTGTCTGCGCGCG
ATCCGCGAAACCGGTGCGGTGAAAGGGCTCGCCCACATCACCGGAGGCGGCTTCACCGACAACATTCCTCGCGTCCTGCC
CGAAGGACTCGGCGTGCGGATCGATCTCGCCGGGTTGACGGTCCTTCCGGTCTTCAAATGGCTGGCGCAGCAGGGTCGCG
TCGTCGAACGCGAGATGCTTCGAACCTTCAATTGCGGCATCGGAATGGTCGCGATCGTGCGCCGCGACGCGGTTCAGCAG
GTCGTGGATGTGCTGACGCAGGCCGGCGAACGTGTGTCGTTGCTGGGCGATGTGATCGTTGCAGGCGATGAAGGCCGGGT
CGTTTACGACGGTCAACTCGATCTCGCCGTATAG

Upstream 100 bases:

>100_bases
AGCCGGGATGTCTTCGAGCGCTGCTAGTGTCCCTTTGATTCCGAAGTTCGTATCAAAGGTTGCGGCAAGGGTATGCGGAC
TTCGGAATCGGGACACTGGC

Downstream 100 bases:

>100_bases
AACGCCCCAGCAGGGAATGTGAGCATGAAACGCCGTGTCGCCATCCTGATCTCCGGACGCGGATCGAACATGACGGCGCT
GGTGGAGGCTGCAAAGGCCG

Product: phosphoribosylaminoimidazole synthetase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTDRNISLTYAGAGVDIDAGNRLVDLIKPMVRATARAGAEAEIGGFGGLFDLKAAGFADPVLVAANDGVGTKVKIAIETG
IHDTIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPETAAAIVAGIAEGCREAGCALIGGETAEMPGLYKDGDYDLAG
FAVGAAERGSLLPGRDIAAGDAVIGLASSGVHSNGYSLVRNIVEASGIALSAPAPFAPVATLGGALLTPTRLYVKSCLRA
IRETGAVKGLAHITGGGFTDNIPRVLPEGLGVRIDLAGLTVLPVFKWLAQQGRVVEREMLRTFNCGIGMVAIVRRDAVQQ
VVDVLTQAGERVSLLGDVIVAGDEGRVVYDGQLDLAV

Sequences:

>Translated_357_residues
MTDRNISLTYAGAGVDIDAGNRLVDLIKPMVRATARAGAEAEIGGFGGLFDLKAAGFADPVLVAANDGVGTKVKIAIETG
IHDTIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPETAAAIVAGIAEGCREAGCALIGGETAEMPGLYKDGDYDLAG
FAVGAAERGSLLPGRDIAAGDAVIGLASSGVHSNGYSLVRNIVEASGIALSAPAPFAPVATLGGALLTPTRLYVKSCLRA
IRETGAVKGLAHITGGGFTDNIPRVLPEGLGVRIDLAGLTVLPVFKWLAQQGRVVEREMLRTFNCGIGMVAIVRRDAVQQ
VVDVLTQAGERVSLLGDVIVAGDEGRVVYDGQLDLAV
>Mature_356_residues
TDRNISLTYAGAGVDIDAGNRLVDLIKPMVRATARAGAEAEIGGFGGLFDLKAAGFADPVLVAANDGVGTKVKIAIETGI
HDTIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPETAAAIVAGIAEGCREAGCALIGGETAEMPGLYKDGDYDLAGF
AVGAAERGSLLPGRDIAAGDAVIGLASSGVHSNGYSLVRNIVEASGIALSAPAPFAPVATLGGALLTPTRLYVKSCLRAI
RETGAVKGLAHITGGGFTDNIPRVLPEGLGVRIDLAGLTVLPVFKWLAQQGRVVEREMLRTFNCGIGMVAIVRRDAVQQV
VDVLTQAGERVSLLGDVIVAGDEGRVVYDGQLDLAV

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family

Homologues:

Organism=Homo sapiens, GI4503915, Length=335, Percent_Identity=57.3134328358209, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI209869995, Length=335, Percent_Identity=57.3134328358209, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI209869993, Length=335, Percent_Identity=57.3134328358209, Blast_Score=381, Evalue=1e-106,
Organism=Escherichia coli, GI1788845, Length=351, Percent_Identity=50.997150997151, Blast_Score=331, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17567511, Length=337, Percent_Identity=47.4777448071217, Blast_Score=290, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6321203, Length=338, Percent_Identity=56.2130177514793, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24582400, Length=292, Percent_Identity=45.2054794520548, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR5_NITWN (Q3SS83)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318210.1
- ProteinModelPortal:   Q3SS83
- SMR:   Q3SS83
- STRING:   Q3SS83
- GeneID:   3675969
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1597
- NMPDR:   fig|323098.3.peg.2259
- eggNOG:   COG0150
- HOGENOM:   HBG531222
- OMA:   HYDLAGF
- PhylomeDB:   Q3SS83
- ProtClustDB:   PRK05385
- BioCyc:   NWIN323098:NWI_1597-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00741_B
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188
- TIGRFAMs:   TIGR00878

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.3.1

Molecular weight: Translated: 36685; Mature: 36553

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRNISLTYAGAGVDIDAGNRLVDLIKPMVRATARAGAEAEIGGFGGLFDLKAAGFADP
CCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCC
VLVAANDGVGTKVKIAIETGIHDTIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPET
EEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCEEEEEHHHCCCCCHHH
AAAIVAGIAEGCREAGCALIGGETAEMPGLYKDGDYDLAGFAVGAAERGSLLPGRDIAAG
HHHHHHHHHHHHHHCCCEEECCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
DAVIGLASSGVHSNGYSLVRNIVEASGIALSAPAPFAPVATLGGALLTPTRLYVKSCLRA
CEEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHCCHHHCHHHHHHHHHHHH
IRETGAVKGLAHITGGGFTDNIPRVLPEGLGVRIDLAGLTVLPVFKWLAQQGRVVEREML
HHHHCHHCCHHEECCCCCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHH
RTFNCGIGMVAIVRRDAVQQVVDVLTQAGERVSLLGDVIVAGDEGRVVYDGQLDLAV
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEECCCEEECC
>Mature Secondary Structure 
TDRNISLTYAGAGVDIDAGNRLVDLIKPMVRATARAGAEAEIGGFGGLFDLKAAGFADP
CCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCC
VLVAANDGVGTKVKIAIETGIHDTIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPET
EEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCEEEEEHHHCCCCCHHH
AAAIVAGIAEGCREAGCALIGGETAEMPGLYKDGDYDLAGFAVGAAERGSLLPGRDIAAG
HHHHHHHHHHHHHHCCCEEECCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
DAVIGLASSGVHSNGYSLVRNIVEASGIALSAPAPFAPVATLGGALLTPTRLYVKSCLRA
CEEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHCCHHHCHHHHHHHHHHHH
IRETGAVKGLAHITGGGFTDNIPRVLPEGLGVRIDLAGLTVLPVFKWLAQQGRVVEREML
HHHHCHHCCHHEECCCCCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHH
RTFNCGIGMVAIVRRDAVQQVVDVLTQAGERVSLLGDVIVAGDEGRVVYDGQLDLAV
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA