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The map label for this gene is kipI [H]

Identifier: 75675693

GI number: 75675693

Start: 1629986

End: 1630720

Strand: Direct

Name: kipI [H]

Synonym: Nwi_1501

Alternate gene names: 75675693

Gene position: 1629986-1630720 (Clockwise)

Preceding gene: 75675691

Following gene: 75675694

Centisome position: 47.91

GC content: 63.54

Gene sequence:

>735_bases
ATGGCTGCGCCGTCCCTAGCCCCTCCTCGCTTGCTGCCGAGCGGCGATACCGCCATTACGGTGGAATTCTGCCGCATCAT
CGATGAGCTGGCCAACCACCGCGTGCTAGCGCTCGATCGTGCGCTTGCGGAGGAACCGATCGAGGGCGTTACGGAAACGG
TGCCAACCTATCGATCGCTGCTCGTTCATTACGATCCTGTCCGGATCGGTTTCGAGGAACTCGCGGAACGCCTGTCGGCG
CTCGCGCGCCTGCCCGTTGCTCCGGCAACTCGCGCCCGGCGCTGGCGAATCCCGGTTGCCTATGGCGGCGAGCACGGCAT
CGATCTGGAAGATGTCGCGAAGACGCTGAACATGACCCCTGACGACATTGTGGCGCGTCATGCCGGCGGCGATTATCGTG
TCGCCATGATCGGATTTACGCCGGGCTGGTCTTATCTGAGCGGTCTCGACAGTTCGCTGCGAATACCGCGGCTGCAAAGT
CCGCGCTTACTGACGCCTTCCGGCATGATCTCCATCGGCGGCCTTCAGGCGGGCATCCAGTGCCTTGGGGGACCCAGCGG
CTGGCGTTTGTTGGGACGAACCGCCGTGCGAACCTATCAGCTTCATCGCGATCCCGTTTTTCTGCTCGAACCCGGAGACG
CCATCACGTTTTACGCCGTCGACGGCAAGCAGTTCGCGGAACAGGAGCGCGCCGCCGCGCACGGCGAGTGGGTCGCGCAA
TTGGTGACGCCATGA

Upstream 100 bases:

>100_bases
GCCACCGAGAATTCATTGGAATTGCATACATCCTTCATGTCAACTCGGCGTTTGAAACGTGTTGCCTTTCATCCGTCACG
AACGAAACCTGTCATCGGTC

Downstream 100 bases:

>100_bases
GCAAACTTGTCGTCACCGCTGTCGGGCCCGTCACCTCGGTTCAGGACGGCGGCCGCTCCGGCGCGCAGCGTTACGGGTTG
CCGCCAGGCGGCGCGGCGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MAAPSLAPPRLLPSGDTAITVEFCRIIDELANHRVLALDRALAEEPIEGVTETVPTYRSLLVHYDPVRIGFEELAERLSA
LARLPVAPATRARRWRIPVAYGGEHGIDLEDVAKTLNMTPDDIVARHAGGDYRVAMIGFTPGWSYLSGLDSSLRIPRLQS
PRLLTPSGMISIGGLQAGIQCLGGPSGWRLLGRTAVRTYQLHRDPVFLLEPGDAITFYAVDGKQFAEQERAAAHGEWVAQ
LVTP

Sequences:

>Translated_244_residues
MAAPSLAPPRLLPSGDTAITVEFCRIIDELANHRVLALDRALAEEPIEGVTETVPTYRSLLVHYDPVRIGFEELAERLSA
LARLPVAPATRARRWRIPVAYGGEHGIDLEDVAKTLNMTPDDIVARHAGGDYRVAMIGFTPGWSYLSGLDSSLRIPRLQS
PRLLTPSGMISIGGLQAGIQCLGGPSGWRLLGRTAVRTYQLHRDPVFLLEPGDAITFYAVDGKQFAEQERAAAHGEWVAQ
LVTP
>Mature_243_residues
AAPSLAPPRLLPSGDTAITVEFCRIIDELANHRVLALDRALAEEPIEGVTETVPTYRSLLVHYDPVRIGFEELAERLSAL
ARLPVAPATRARRWRIPVAYGGEHGIDLEDVAKTLNMTPDDIVARHAGGDYRVAMIGFTPGWSYLSGLDSSLRIPRLQSP
RLLTPSGMISIGGLQAGIQCLGGPSGWRLLGRTAVRTYQLHRDPVFLLEPGDAITFYAVDGKQFAEQERAAAHGEWVAQL
VTP

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=204, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26596; Mature: 26465

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPSLAPPRLLPSGDTAITVEFCRIIDELANHRVLALDRALAEEPIEGVTETVPTYRSL
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LVHYDPVRIGFEELAERLSALARLPVAPATRARRWRIPVAYGGEHGIDLEDVAKTLNMTP
HHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECEEEECCCCCCCHHHHHHHHCCCH
DDIVARHAGGDYRVAMIGFTPGWSYLSGLDSSLRIPRLQSPRLLTPSGMISIGGLQAGIQ
HHHHHHCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCCCCCEECCCCCEEECCHHHHHH
CLGGPSGWRLLGRTAVRTYQLHRDPVFLLEPGDAITFYAVDGKQFAEQERAAAHGEWVAQ
CCCCCCHHHHHHHHHHHHEEECCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
LVTP
HCCC
>Mature Secondary Structure 
AAPSLAPPRLLPSGDTAITVEFCRIIDELANHRVLALDRALAEEPIEGVTETVPTYRSL
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LVHYDPVRIGFEELAERLSALARLPVAPATRARRWRIPVAYGGEHGIDLEDVAKTLNMTP
HHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECEEEECCCCCCCHHHHHHHHCCCH
DDIVARHAGGDYRVAMIGFTPGWSYLSGLDSSLRIPRLQSPRLLTPSGMISIGGLQAGIQ
HHHHHHCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCCCCCEECCCCCEEECCHHHHHH
CLGGPSGWRLLGRTAVRTYQLHRDPVFLLEPGDAITFYAVDGKQFAEQERAAAHGEWVAQ
CCCCCCHHHHHHHHHHHHEEECCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHH
LVTP
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]