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The map label for this gene is potF [H]

Identifier: 75675434

GI number: 75675434

Start: 1365734

End: 1366849

Strand: Direct

Name: potF [H]

Synonym: Nwi_1241

Alternate gene names: 75675434

Gene position: 1365734-1366849 (Clockwise)

Preceding gene: 75675433

Following gene: 75675448

Centisome position: 40.14

GC content: 57.44

Gene sequence:

>1116_bases
ATGATTCCGAACCAATGCGTGTGGTTCTGGGCGCCCGTTGCATTGATGCTTGGTTCGGGCGCGGTCTTGTCGCCGCGCGC
CGACGCCGCCGAGCGCGTCGTCAACTTTTTCAACTGGTCGAACTACATCGCGCCGGGCGTGCTCGAGGCCTTCACCGAGG
AAACTGGAATCAAGGTGGTCTACGACACCTTCGACGCCAACGAGACGCTCGAAACGCGGCTCCTTGCCGGCAAGTCCGGT
TACGATATCGTGGTGCCGACGGCGTATTTTTTGCAGCGTCAGATCGGCGCGAACATCTTTCGGAAACTCGACAAGTCGAA
GCTGCCGAATCTGGCCAACGCATGGCCCATGGTGACGGAGAGGCTCGCAACCTATGATCCCGGCAATCAGTACGCCGTGA
ATTACATGTGGGGCACGACCGGCATCGGCTACAATGTCAAAGCAGTGCGGCGCATTCTCGGACCCGACAGCAGGATTGAT
AGTTGGGATGTCATCTTCGATCCGGACAAGCTCGCGAAGTTCAAGGACTGCGGCGTCCAGATGCTTGATTCCGCGGATGA
TATCCTGCCTGCCGCGCTCAACTTTCTCGGCCTCGATCCGAACTCGACAGAAAAGAGCGATCTCGAACAGGCAGCGGCTC
TTGTCGGCAAGATCAGGCCGTATGTCCGCAAGTTTCATTCCTCGGAATATCTTAACAGCCTAGCCACTGGAGAGATCTGC
CTTGTCGTCGGCTGGTCCGGCGATATCAAGCAGGCCCAAAGCCGCGCGGCGGAGAGCCATAACGGTGTCGAGATCGGTTA
TGCGATTCCGAAAGAGGGCGCCCAGATGTTCTTCGACAATCTGGCCGTTCTCCTGGATGCCAGGCATGTCGACGAGGCTT
ACGAGTTGATCAATTATCTCTATCGGCCGGACGTTGCGGCGAAGAATTCGGACTTCCTGTCCTATGCCAGCGGCAATCTG
GCCAGCCAGAAGCTGATCGATCCGCAAGCCTTCAATGACAAGACGATCTATCCGGATGAAGCCGTGATGAAGCGGCTGTT
CATCATAACTGCGCGCGATCTCGCGACGCAACGTTCGATCAACCGGCTCTGGACGCGCATAAAGACGGGACGGTGA

Upstream 100 bases:

>100_bases
ACGCGGTTACCTCGGTCGTACGTTTCTGTTTCGCGAAGAAGGATGCCACGCTCGACGCTGCGCTGGAGCGGCTATCCGAC
GCCGTGCGGAGGTGAGGCCG

Downstream 100 bases:

>100_bases
CAGGACGGAGAACTTTCTTCACGCGAACCGAATCCCGCTTCGCTCGAAAACGCCTTAACGCCAGCGGCGGTGCAGCCACA
GCCATTGATCGGGATATTCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIPNQCVWFWAPVALMLGSGAVLSPRADAAERVVNFFNWSNYIAPGVLEAFTEETGIKVVYDTFDANETLETRLLAGKSG
YDIVVPTAYFLQRQIGANIFRKLDKSKLPNLANAWPMVTERLATYDPGNQYAVNYMWGTTGIGYNVKAVRRILGPDSRID
SWDVIFDPDKLAKFKDCGVQMLDSADDILPAALNFLGLDPNSTEKSDLEQAAALVGKIRPYVRKFHSSEYLNSLATGEIC
LVVGWSGDIKQAQSRAAESHNGVEIGYAIPKEGAQMFFDNLAVLLDARHVDEAYELINYLYRPDVAAKNSDFLSYASGNL
ASQKLIDPQAFNDKTIYPDEAVMKRLFIITARDLATQRSINRLWTRIKTGR

Sequences:

>Translated_371_residues
MIPNQCVWFWAPVALMLGSGAVLSPRADAAERVVNFFNWSNYIAPGVLEAFTEETGIKVVYDTFDANETLETRLLAGKSG
YDIVVPTAYFLQRQIGANIFRKLDKSKLPNLANAWPMVTERLATYDPGNQYAVNYMWGTTGIGYNVKAVRRILGPDSRID
SWDVIFDPDKLAKFKDCGVQMLDSADDILPAALNFLGLDPNSTEKSDLEQAAALVGKIRPYVRKFHSSEYLNSLATGEIC
LVVGWSGDIKQAQSRAAESHNGVEIGYAIPKEGAQMFFDNLAVLLDARHVDEAYELINYLYRPDVAAKNSDFLSYASGNL
ASQKLIDPQAFNDKTIYPDEAVMKRLFIITARDLATQRSINRLWTRIKTGR
>Mature_371_residues
MIPNQCVWFWAPVALMLGSGAVLSPRADAAERVVNFFNWSNYIAPGVLEAFTEETGIKVVYDTFDANETLETRLLAGKSG
YDIVVPTAYFLQRQIGANIFRKLDKSKLPNLANAWPMVTERLATYDPGNQYAVNYMWGTTGIGYNVKAVRRILGPDSRID
SWDVIFDPDKLAKFKDCGVQMLDSADDILPAALNFLGLDPNSTEKSDLEQAAALVGKIRPYVRKFHSSEYLNSLATGEIC
LVVGWSGDIKQAQSRAAESHNGVEIGYAIPKEGAQMFFDNLAVLLDARHVDEAYELINYLYRPDVAAKNSDFLSYASGNL
ASQKLIDPQAFNDKTIYPDEAVMKRLFIITARDLATQRSINRLWTRIKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=359, Percent_Identity=47.075208913649, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1787367, Length=338, Percent_Identity=35.7988165680473, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41374; Mature: 41374

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPNQCVWFWAPVALMLGSGAVLSPRADAAERVVNFFNWSNYIAPGVLEAFTEETGIKVV
CCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEE
YDTFDANETLETRLLAGKSGYDIVVPTAYFLQRQIGANIFRKLDKSKLPNLANAWPMVTE
EECCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RLATYDPGNQYAVNYMWGTTGIGYNVKAVRRILGPDSRIDSWDVIFDPDKLAKFKDCGVQ
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHCCHH
MLDSADDILPAALNFLGLDPNSTEKSDLEQAAALVGKIRPYVRKFHSSEYLNSLATGEIC
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVVGWSGDIKQAQSRAAESHNGVEIGYAIPKEGAQMFFDNLAVLLDARHVDEAYELINYL
EEEECCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRPDVAAKNSDFLSYASGNLASQKLIDPQAFNDKTIYPDEAVMKRLFIITARDLATQRSI
HCCCCCCCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NRLWTRIKTGR
HHHHHHHCCCC
>Mature Secondary Structure
MIPNQCVWFWAPVALMLGSGAVLSPRADAAERVVNFFNWSNYIAPGVLEAFTEETGIKVV
CCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEE
YDTFDANETLETRLLAGKSGYDIVVPTAYFLQRQIGANIFRKLDKSKLPNLANAWPMVTE
EECCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RLATYDPGNQYAVNYMWGTTGIGYNVKAVRRILGPDSRIDSWDVIFDPDKLAKFKDCGVQ
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHCCHH
MLDSADDILPAALNFLGLDPNSTEKSDLEQAAALVGKIRPYVRKFHSSEYLNSLATGEIC
HHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVVGWSGDIKQAQSRAAESHNGVEIGYAIPKEGAQMFFDNLAVLLDARHVDEAYELINYL
EEEECCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRPDVAAKNSDFLSYASGNLASQKLIDPQAFNDKTIYPDEAVMKRLFIITARDLATQRSI
HCCCCCCCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NRLWTRIKTGR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]