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The map label for this gene is hldE

Identifier: 75675264

GI number: 75675264

Start: 1171471

End: 1172937

Strand: Direct

Name: hldE

Synonym: Nwi_1071

Alternate gene names: 75675264

Gene position: 1171471-1172937 (Clockwise)

Preceding gene: 75675263

Following gene: 75675265

Centisome position: 34.43

GC content: 64.96

Gene sequence:

>1467_bases
ATGATCGATTTTGACGGCTTGTCTAATGCGTTTTCCGGCCAGACGGTGCTCTGTGTCGGCGACCTGATGCTCGACGAGTT
CGTGTACGGCGAAATTTCGCGGATTTCGCCGGAGGCTCCGGCCCCGGTCATCGCGGTTCAGCGAAGCGAAACCAACGTCG
GCGGGGCCGGCAATGTGGCGCGCAACATCGCCGCGCTCGGCGCGCGCTGCATCTTCGTCGGATTGGCCGGAGAGGATGAC
GCGGGCGCGAGGCTGCGGGACACGCTTTCGCGGGAACGCCTGATCGAGCCGTTGTTGATTTCAGATCCGGCGCGCTCGAC
GACGCGCAAGGTGCGTTTCGTTTCGGAGCATTTTTCGACCCACATGCTGCGCGCGGATTGGGAAACGCTTGCTCCGGCGT
CGGGGGAGGTGGAGCAGGCCCTGATCGATGCGATTCTGCCGCAACTGCCATGCGCCGACATCGTATTGTTGTCCGACTAT
GCCAAGGGAGTGCTGACCACGCGGGTGATCCGCAGCGTCATCGACGGCGCGCGCCGGTTGGGCAAGCGCGTGATCGTCGA
TCCGAAGAGCGCCGATTTCGGCATCTATCGCGGCGCGACGCTCCTCACGCCAAACAGCAAGGAATTCACGGACGCAACCG
GTCGCCGCGCGGATGATCAGGCAAGCCTTGCGAAGGCTGCGCGAGACCTGATGCGGATCGCGGAATGCGAAGCCTTGCTG
GTGACGCAAAGCGAGCGCGGCATGACGCTCGTGCCGGGCGAGGGCGAGGTCATTCATGTTCCCGCTCACACCGTCAAGGT
CCGCGATGTGACGGGCGCCGGCGACACCGTCGTCGCCACGCTCGCGGTTTCGCTCGCGGCGGGCGCGGACTGGGAAGCCG
CGTTGCGGACGGCGAGCGCGGCGGCCGCCGTCGCTGTCGCCAAGAGTGGCACCGCGATCGTCACGCTCGCGGAACTGCGC
CGCAATATCCTGCCGCCGGCCTCCCTGGCCGCCGAGGAGAAGATCGCGCGATCGGCAGGCGACCTTGATCAACACCTGAC
GGCATGGCGTGAACAGGGTCTGCGGATCGGCTTCACCAATGGCTGCTTCGATATCCTGCATCCCGGCCACGTCAAGGTGC
TGACCGGCGCGCGGGCTGCGTGCGATCGGCTGATTGTCGGATTGAACAGCGATGCGTCCGTGAAGCGGCTGAAGGGCGAG
GGCCGTCCGATCCAGAACGAGCGCGCGCGCGCGGAAGTGCTTGCCGCGCTCGAAGCCGTCGATCTCGTCGCGATCTTCGA
GGAAGACACGCCGCTGAACCTGATCGGGCGAATCCAGCCGAACGTGCTCGTCAAGGGCGGGGATTATTCTCTTGAGCAAG
TCGTCGGTCAGGAGCTAGTGACTGCACGAGGCGGCGAGGTTATTCTGATCGACATCCTGCAAGGATTCAGCACGACATCG
CTGGTGAAGCGCGCCGGAGGGGCATGA

Upstream 100 bases:

>100_bases
TCAGAAGCGGGGTTCTATGATATTGTTTTGACGCGTTTTCTTCACGCGAACAGGTCATCCACTTCGCTCGAAAACGCTCT
AGCAACGAGTGAAGGGTTTG

Downstream 100 bases:

>100_bases
TCGATCGTGTTCGACCGGCGCTTCCCCGTATCCCAATGACTTTCCGCCTCTGACGATCTGGACGACGTATGAACCTCCGC
CGAAAATTCACGCTCCGAAA

Product: cytidyltransferase-related, RfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MIDFDGLSNAFSGQTVLCVGDLMLDEFVYGEISRISPEAPAPVIAVQRSETNVGGAGNVARNIAALGARCIFVGLAGEDD
AGARLRDTLSRERLIEPLLISDPARSTTRKVRFVSEHFSTHMLRADWETLAPASGEVEQALIDAILPQLPCADIVLLSDY
AKGVLTTRVIRSVIDGARRLGKRVIVDPKSADFGIYRGATLLTPNSKEFTDATGRRADDQASLAKAARDLMRIAECEALL
VTQSERGMTLVPGEGEVIHVPAHTVKVRDVTGAGDTVVATLAVSLAAGADWEAALRTASAAAAVAVAKSGTAIVTLAELR
RNILPPASLAAEEKIARSAGDLDQHLTAWREQGLRIGFTNGCFDILHPGHVKVLTGARAACDRLIVGLNSDASVKRLKGE
GRPIQNERARAEVLAALEAVDLVAIFEEDTPLNLIGRIQPNVLVKGGDYSLEQVVGQELVTARGGEVILIDILQGFSTTS
LVKRAGGA

Sequences:

>Translated_488_residues
MIDFDGLSNAFSGQTVLCVGDLMLDEFVYGEISRISPEAPAPVIAVQRSETNVGGAGNVARNIAALGARCIFVGLAGEDD
AGARLRDTLSRERLIEPLLISDPARSTTRKVRFVSEHFSTHMLRADWETLAPASGEVEQALIDAILPQLPCADIVLLSDY
AKGVLTTRVIRSVIDGARRLGKRVIVDPKSADFGIYRGATLLTPNSKEFTDATGRRADDQASLAKAARDLMRIAECEALL
VTQSERGMTLVPGEGEVIHVPAHTVKVRDVTGAGDTVVATLAVSLAAGADWEAALRTASAAAAVAVAKSGTAIVTLAELR
RNILPPASLAAEEKIARSAGDLDQHLTAWREQGLRIGFTNGCFDILHPGHVKVLTGARAACDRLIVGLNSDASVKRLKGE
GRPIQNERARAEVLAALEAVDLVAIFEEDTPLNLIGRIQPNVLVKGGDYSLEQVVGQELVTARGGEVILIDILQGFSTTS
LVKRAGGA
>Mature_488_residues
MIDFDGLSNAFSGQTVLCVGDLMLDEFVYGEISRISPEAPAPVIAVQRSETNVGGAGNVARNIAALGARCIFVGLAGEDD
AGARLRDTLSRERLIEPLLISDPARSTTRKVRFVSEHFSTHMLRADWETLAPASGEVEQALIDAILPQLPCADIVLLSDY
AKGVLTTRVIRSVIDGARRLGKRVIVDPKSADFGIYRGATLLTPNSKEFTDATGRRADDQASLAKAARDLMRIAECEALL
VTQSERGMTLVPGEGEVIHVPAHTVKVRDVTGAGDTVVATLAVSLAAGADWEAALRTASAAAAVAVAKSGTAIVTLAELR
RNILPPASLAAEEKIARSAGDLDQHLTAWREQGLRIGFTNGCFDILHPGHVKVLTGARAACDRLIVGLNSDASVKRLKGE
GRPIQNERARAEVLAALEAVDLVAIFEEDTPLNLIGRIQPNVLVKGGDYSLEQVVGQELVTARGGEVILIDILQGFSTTS
LVKRAGGA

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=479, Percent_Identity=44.258872651357, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_NITWN (Q3STQ8)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317685.1
- ProteinModelPortal:   Q3STQ8
- SMR:   Q3STQ8
- STRING:   Q3STQ8
- GeneID:   3674268
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1071
- NMPDR:   fig|323098.3.peg.1773
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- PhylomeDB:   Q3STQ8
- ProtClustDB:   CLSK820002
- BioCyc:   NWIN323098:NWI_1071-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 51680; Mature: 51680

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFDGLSNAFSGQTVLCVGDLMLDEFVYGEISRISPEAPAPVIAVQRSETNVGGAGNVA
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHH
RNIAALGARCIFVGLAGEDDAGARLRDTLSRERLIEPLLISDPARSTTRKVRFVSEHFST
HHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHH
HMLRADWETLAPASGEVEQALIDAILPQLPCADIVLLSDYAKGVLTTRVIRSVIDGARRL
HHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
GKRVIVDPKSADFGIYRGATLLTPNSKEFTDATGRRADDQASLAKAARDLMRIAECEALL
CCEEEECCCCCCCCEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VTQSERGMTLVPGEGEVIHVPAHTVKVRDVTGAGDTVVATLAVSLAAGADWEAALRTASA
EEECCCCEEEECCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AAAVAVAKSGTAIVTLAELRRNILPPASLAAEEKIARSAGDLDQHLTAWREQGLRIGFTN
HHHHEEECCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECC
GCFDILHPGHVKVLTGARAACDRLIVGLNSDASVKRLKGEGRPIQNERARAEVLAALEAV
CCHHHCCCCCEEEEECCHHHHHHEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
DLVAIFEEDTPLNLIGRIQPNVLVKGGDYSLEQVVGQELVTARGGEVILIDILQGFSTTS
HEEEEECCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
LVKRAGGA
HHHHCCCC
>Mature Secondary Structure
MIDFDGLSNAFSGQTVLCVGDLMLDEFVYGEISRISPEAPAPVIAVQRSETNVGGAGNVA
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHH
RNIAALGARCIFVGLAGEDDAGARLRDTLSRERLIEPLLISDPARSTTRKVRFVSEHFST
HHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHH
HMLRADWETLAPASGEVEQALIDAILPQLPCADIVLLSDYAKGVLTTRVIRSVIDGARRL
HHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
GKRVIVDPKSADFGIYRGATLLTPNSKEFTDATGRRADDQASLAKAARDLMRIAECEALL
CCEEEECCCCCCCCEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VTQSERGMTLVPGEGEVIHVPAHTVKVRDVTGAGDTVVATLAVSLAAGADWEAALRTASA
EEECCCCEEEECCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AAAVAVAKSGTAIVTLAELRRNILPPASLAAEEKIARSAGDLDQHLTAWREQGLRIGFTN
HHHHEEECCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECC
GCFDILHPGHVKVLTGARAACDRLIVGLNSDASVKRLKGEGRPIQNERARAEVLAALEAV
CCHHHCCCCCEEEEECCHHHHHHEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
DLVAIFEEDTPLNLIGRIQPNVLVKGGDYSLEQVVGQELVTARGGEVILIDILQGFSTTS
HEEEEECCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
LVKRAGGA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA