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The map label for this gene is ddl [H]

Identifier: 75675248

GI number: 75675248

Start: 1150386

End: 1151378

Strand: Direct

Name: ddl [H]

Synonym: Nwi_1055

Alternate gene names: 75675248

Gene position: 1150386-1151378 (Clockwise)

Preceding gene: 75675247

Following gene: 75675249

Centisome position: 33.81

GC content: 65.26

Gene sequence:

>993_bases
ATGCGCATCACCATTCTGTTCGGCGGCAGCAACAAGGAGCGTCTGGTTTCAGTCGCGAGCGCGCAGGCGCTCCATCACGC
ATTGCCGGAGGCCGATCTGTGGTTCTGGAACCTTCGCGATGAAATCGTCGACGTGCGATCCGAAACGCTGCGGGATCATG
CGCGGCCGTTCGAGGACGAGTTCGTTCCCGGCGGATCAGGCGCGCCGCTGGAGCGGGCGCTGGACAGGGCCAGGGCGGAG
GATCGCGTGCTGGTGCTCGGCCTCCACGGAGGCCGCGCCGAGAACGGTGAACTTCAGGCGATGTGCGAGCTTCGCCGAAT
TCCTTTCACGGGATCGGGTTCGGCCGCCTCGCATCTTGCGTTCGACAAGGTGGCGGCCAAGCGGTTCGTGGCCTCGGCCG
GGGTCCCCACGCCGGCGCACGTCTTGCTGGAGGACATTGACCACGCGCTGGCCGAACACGGCCGGCTGGTTGCCAAGCCC
GCATGCGACGGATCGAGCTATGGCCTGATCTTCGTCAACGCGCCGCAGGATATCGTCACCGTCCGCGAGGCGGCGAAGAC
CGAGGACTATGTGATCGAACCTTTCGTGTCTGGCGTCGAGGCGACCTGCGGCGTGCTGGAGCATTCCGACGGCTCGGTCG
TCGCGCTTCCGCCGGTCGAGATCGTGCCGGCCGAGGGCGCGTTCGATTACGCCGCGAAGTATCTGGCGAAGACGACGCAG
GAGATCTGTCCGGGACGTTTTTCGCCGGAGATCGCCGCGCAGATCATGGATCACTCGCTGCGGGCGCATCGCGCGTTGTC
CTGCCGCGGCTATTCCCGCACCGACTTTATCGTGACAGGCAATGGACCGATCTATCTGGAAACCAACACCCTGCCGGGCC
TGACCGCGGCCTCGCTTTACCCCAAGGCGCTTCATGCGCAGGGGGTCGGGTTCGCTGATTTTCTGCGCGACCAGATCGTG
CTCGCCGAAAAGGCCGTCCGTCCGAACCTCTAG

Upstream 100 bases:

>100_bases
ACATCGAAACCCTGGGGGAGAGCGTACGCGAGCGGGTAAAACGGCATTCCGGTGTCGATCTGCAATGGGAAATCAAACGG
ATCGGATTGGGATAGGGCTC

Downstream 100 bases:

>100_bases
CGGTTTCTTGGGACGCGGGAACCGGTTCGCCCCCGGCGGGAACCTGCCGGAGCCCCTCGGGTCCATCCGGCAGGTTGGCC
CGAAAGTCGCAAGACATTCT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRITILFGGSNKERLVSVASAQALHHALPEADLWFWNLRDEIVDVRSETLRDHARPFEDEFVPGGSGAPLERALDRARAE
DRVLVLGLHGGRAENGELQAMCELRRIPFTGSGSAASHLAFDKVAAKRFVASAGVPTPAHVLLEDIDHALAEHGRLVAKP
ACDGSSYGLIFVNAPQDIVTVREAAKTEDYVIEPFVSGVEATCGVLEHSDGSVVALPPVEIVPAEGAFDYAAKYLAKTTQ
EICPGRFSPEIAAQIMDHSLRAHRALSCRGYSRTDFIVTGNGPIYLETNTLPGLTAASLYPKALHAQGVGFADFLRDQIV
LAEKAVRPNL

Sequences:

>Translated_330_residues
MRITILFGGSNKERLVSVASAQALHHALPEADLWFWNLRDEIVDVRSETLRDHARPFEDEFVPGGSGAPLERALDRARAE
DRVLVLGLHGGRAENGELQAMCELRRIPFTGSGSAASHLAFDKVAAKRFVASAGVPTPAHVLLEDIDHALAEHGRLVAKP
ACDGSSYGLIFVNAPQDIVTVREAAKTEDYVIEPFVSGVEATCGVLEHSDGSVVALPPVEIVPAEGAFDYAAKYLAKTTQ
EICPGRFSPEIAAQIMDHSLRAHRALSCRGYSRTDFIVTGNGPIYLETNTLPGLTAASLYPKALHAQGVGFADFLRDQIV
LAEKAVRPNL
>Mature_330_residues
MRITILFGGSNKERLVSVASAQALHHALPEADLWFWNLRDEIVDVRSETLRDHARPFEDEFVPGGSGAPLERALDRARAE
DRVLVLGLHGGRAENGELQAMCELRRIPFTGSGSAASHLAFDKVAAKRFVASAGVPTPAHVLLEDIDHALAEHGRLVAKP
ACDGSSYGLIFVNAPQDIVTVREAAKTEDYVIEPFVSGVEATCGVLEHSDGSVVALPPVEIVPAEGAFDYAAKYLAKTTQ
EICPGRFSPEIAAQIMDHSLRAHRALSCRGYSRTDFIVTGNGPIYLETNTLPGLTAASLYPKALHAQGVGFADFLRDQIV
LAEKAVRPNL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=338, Percent_Identity=31.9526627218935, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=28.2913165266106, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 35581; Mature: 35581

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITILFGGSNKERLVSVASAQALHHALPEADLWFWNLRDEIVDVRSETLRDHARPFEDE
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHH
FVPGGSGAPLERALDRARAEDRVLVLGLHGGRAENGELQAMCELRRIPFTGSGSAASHLA
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
FDKVAAKRFVASAGVPTPAHVLLEDIDHALAEHGRLVAKPACDGSSYGLIFVNAPQDIVT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCHHHHH
VREAAKTEDYVIEPFVSGVEATCGVLEHSDGSVVALPPVEIVPAEGAFDYAAKYLAKTTQ
HHHHHCCCCEEEHHHHHCHHHHHHHEECCCCCEEEECCEEEEECCCCHHHHHHHHHHHHH
EICPGRFSPEIAAQIMDHSLRAHRALSCRGYSRTDFIVTGNGPIYLETNTLPGLTAASLY
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHC
PKALHAQGVGFADFLRDQIVLAEKAVRPNL
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRITILFGGSNKERLVSVASAQALHHALPEADLWFWNLRDEIVDVRSETLRDHARPFEDE
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHH
FVPGGSGAPLERALDRARAEDRVLVLGLHGGRAENGELQAMCELRRIPFTGSGSAASHLA
CCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
FDKVAAKRFVASAGVPTPAHVLLEDIDHALAEHGRLVAKPACDGSSYGLIFVNAPQDIVT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCHHHHH
VREAAKTEDYVIEPFVSGVEATCGVLEHSDGSVVALPPVEIVPAEGAFDYAAKYLAKTTQ
HHHHHCCCCEEEHHHHHCHHHHHHHEECCCCCEEEECCEEEEECCCCHHHHHHHHHHHHH
EICPGRFSPEIAAQIMDHSLRAHRALSCRGYSRTDFIVTGNGPIYLETNTLPGLTAASLY
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHC
PKALHAQGVGFADFLRDQIVLAEKAVRPNL
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]