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The map label for this gene is murD

Identifier: 75675242

GI number: 75675242

Start: 1143542

End: 1144942

Strand: Direct

Name: murD

Synonym: Nwi_1049

Alternate gene names: 75675242

Gene position: 1143542-1144942 (Clockwise)

Preceding gene: 75675241

Following gene: 75675243

Centisome position: 33.61

GC content: 67.52

Gene sequence:

>1401_bases
ATGATCCCCGTCACATCCTTTTCCGGCAAGACCGTCGCGGTATTTGGCCTCGGCGGTTCGGGCCTTGCGAGTTGTCACGC
CCTGAAGGCGGGCGGCGCCGAGGTGATTGCGGGCGACGACAACGCCGACAATCTCGCGAAGGCGGCGCAGGCCGGTTTCA
TCACCGCGGATTTGCGAACCGTGGTGTGGGCGAACCTCGCCGCGCTGGTGCTTGCGCCGGGCGTGCCGCTGACGCATCCC
TCGCCGCACTGGAGCGTGCTGGCGGCGCGGCAGGCGGGCGTCGAGGTGATCGGCGACGTCGAACTGTTCTGCCGCGAGCG
CGCACGTCATGCGCCCGACGCGCCTTTCATCGCCATCACCGGCACCAACGGCAAGTCCACTACGACGGCGCTGGTCGCGC
ATCTGATGCGCGAGGCCGGATACGACGTGCAGATGGGCGGCAACATCGGCACCGCGATCCTGTCGCTGGAGCCGCCGCGC
AGAGGGCGCGTGCATGTGATCGAGATGTCGTCGTACCAGATCGACCTCGCGCCCTCGCTCGATCCGACCGTCGGCATTCT
GCTCAACCTCAGTCCCGATCACCTCGATCGCCACGGCACCATCGAACATTATGCGCAGGTGAAGGAGAGGCTCATCGCCG
GGGTGCAGCCGCAGGGCACGGCGGTCGTCGGCGTCGATGATCACTGGTGCGCGGCGATCGCCGACCGTCAGGAGCAGGCC
GGACGCAGCGTCGTGCGCGTGTCGGTGAAGCGGCCACTGGCGCATGGCGTCAGCGTCGAACAGGACAGGATCGTTCTCGT
GTCCGGCGGCGCGCGCAGCGAAATCGCCGACATCGGCGGAATAGGCTCGCTGCGCGGGCGGCACAACGCGCAGAATGCGG
CCTGCGCTTCGGCGGGGGCGCTGGCGCTCGGGGTCAGCCGGGACATCCTGCAGCAGGACTTGCGCAGTTTTCCCGGTCTC
GCGCATCGCATGGAGCAGGTGGGGCGCAAGGCCCATGTGCTGTTCGTCAACGACTCCAAGGGCACCAACGCCGACGCCAC
CGAGAAGGCGTTGTCGTCCTTCGACGAGATTTTCTGGATCGCGGGCGGCAAGCCGAAAAGCGGCGGTATCGCCTCGCTCG
ACGCGTTCTTTCCGCGCATCCGCAAAGCCTATCTGATCGGAGAGGCGGCGCAGGAGTTCGCGGCGACGCTGGAGGGCAAG
GTGGCCTACGAGATCAGCGGAACGCTGGAAGCCGCGGTGCCCGCCGCCGCGCGGGACGCGGAAGGAGCAGGCCGTGCCGG
GGCGGTGGTGCTGTTGTCGCCGGCCTGCGCTTCATTCGATCAGTTTCGCAATTTTGAAGTTCGCGGCGATCGCTTTCGCG
AACTGGTCCAGGCGTTGCCGGGCGTTACGCCCGTCGTGTAG

Upstream 100 bases:

>100_bases
GGCTGGACCGAGCCGCAGATCGTGATCAGGTTCTGGATCATTTCGGTGATGCTGGCGCTGGCGGGTCTTTCGACGCTGAA
GTTGCGGTGATGAAGCCCCC

Downstream 100 bases:

>100_bases
CTCGCGCCGCCCTCGTCATGGCCGGGCATAGCCGTTCGAAGAACGGCGTCGCTTCCGCTCGCCCATGGTCCCGGCCATCC
ACGTCTTCCTCCTTGATGCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIPVTSFSGKTVAVFGLGGSGLASCHALKAGGAEVIAGDDNADNLAKAAQAGFITADLRTVVWANLAALVLAPGVPLTHP
SPHWSVLAARQAGVEVIGDVELFCRERARHAPDAPFIAITGTNGKSTTTALVAHLMREAGYDVQMGGNIGTAILSLEPPR
RGRVHVIEMSSYQIDLAPSLDPTVGILLNLSPDHLDRHGTIEHYAQVKERLIAGVQPQGTAVVGVDDHWCAAIADRQEQA
GRSVVRVSVKRPLAHGVSVEQDRIVLVSGGARSEIADIGGIGSLRGRHNAQNAACASAGALALGVSRDILQQDLRSFPGL
AHRMEQVGRKAHVLFVNDSKGTNADATEKALSSFDEIFWIAGGKPKSGGIASLDAFFPRIRKAYLIGEAAQEFAATLEGK
VAYEISGTLEAAVPAAARDAEGAGRAGAVVLLSPACASFDQFRNFEVRGDRFRELVQALPGVTPVV

Sequences:

>Translated_466_residues
MIPVTSFSGKTVAVFGLGGSGLASCHALKAGGAEVIAGDDNADNLAKAAQAGFITADLRTVVWANLAALVLAPGVPLTHP
SPHWSVLAARQAGVEVIGDVELFCRERARHAPDAPFIAITGTNGKSTTTALVAHLMREAGYDVQMGGNIGTAILSLEPPR
RGRVHVIEMSSYQIDLAPSLDPTVGILLNLSPDHLDRHGTIEHYAQVKERLIAGVQPQGTAVVGVDDHWCAAIADRQEQA
GRSVVRVSVKRPLAHGVSVEQDRIVLVSGGARSEIADIGGIGSLRGRHNAQNAACASAGALALGVSRDILQQDLRSFPGL
AHRMEQVGRKAHVLFVNDSKGTNADATEKALSSFDEIFWIAGGKPKSGGIASLDAFFPRIRKAYLIGEAAQEFAATLEGK
VAYEISGTLEAAVPAAARDAEGAGRAGAVVLLSPACASFDQFRNFEVRGDRFRELVQALPGVTPVV
>Mature_466_residues
MIPVTSFSGKTVAVFGLGGSGLASCHALKAGGAEVIAGDDNADNLAKAAQAGFITADLRTVVWANLAALVLAPGVPLTHP
SPHWSVLAARQAGVEVIGDVELFCRERARHAPDAPFIAITGTNGKSTTTALVAHLMREAGYDVQMGGNIGTAILSLEPPR
RGRVHVIEMSSYQIDLAPSLDPTVGILLNLSPDHLDRHGTIEHYAQVKERLIAGVQPQGTAVVGVDDHWCAAIADRQEQA
GRSVVRVSVKRPLAHGVSVEQDRIVLVSGGARSEIADIGGIGSLRGRHNAQNAACASAGALALGVSRDILQQDLRSFPGL
AHRMEQVGRKAHVLFVNDSKGTNADATEKALSSFDEIFWIAGGKPKSGGIASLDAFFPRIRKAYLIGEAAQEFAATLEGK
VAYEISGTLEAAVPAAARDAEGAGRAGAVVLLSPACASFDQFRNFEVRGDRFRELVQALPGVTPVV

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=466, Percent_Identity=33.4763948497854, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_NITWN (Q3STT0)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317663.1
- ProteinModelPortal:   Q3STT0
- SMR:   Q3STT0
- STRING:   Q3STT0
- GeneID:   3674872
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1049
- NMPDR:   fig|323098.3.peg.2992
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q3STT0
- ProtClustDB:   PRK01390
- BioCyc:   NWIN323098:NWI_1049-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48760; Mature: 48760

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTSFSGKTVAVFGLGGSGLASCHALKAGGAEVIAGDDNADNLAKAAQAGFITADLRT
CCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCEEEHHHHH
VVWANLAALVLAPGVPLTHPSPHWSVLAARQAGVEVIGDVELFCRERARHAPDAPFIAIT
HHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHCCCEEECHHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALVAHLMREAGYDVQMGGNIGTAILSLEPPRRGRVHVIEMSSYQIDLAPSL
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPTVGILLNLSPDHLDRHGTIEHYAQVKERLIAGVQPQGTAVVGVDDHWCAAIADRQEQA
CCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHC
GRSVVRVSVKRPLAHGVSVEQDRIVLVSGGARSEIADIGGIGSLRGRHNAQNAACASAGA
CCEEEEEEEHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHCCCC
LALGVSRDILQQDLRSFPGLAHRMEQVGRKAHVLFVNDSKGTNADATEKALSSFDEIFWI
EEECCCHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEE
AGGKPKSGGIASLDAFFPRIRKAYLIGEAAQEFAATLEGKVAYEISGTLEAAVPAAARDA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCC
EGAGRAGAVVLLSPACASFDQFRNFEVRGDRFRELVQALPGVTPVV
CCCCCCCEEEEECCCHHCHHHHCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIPVTSFSGKTVAVFGLGGSGLASCHALKAGGAEVIAGDDNADNLAKAAQAGFITADLRT
CCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCEEEHHHHH
VVWANLAALVLAPGVPLTHPSPHWSVLAARQAGVEVIGDVELFCRERARHAPDAPFIAIT
HHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHCCCEEECHHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALVAHLMREAGYDVQMGGNIGTAILSLEPPRRGRVHVIEMSSYQIDLAPSL
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPTVGILLNLSPDHLDRHGTIEHYAQVKERLIAGVQPQGTAVVGVDDHWCAAIADRQEQA
CCCEEEEEECCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHC
GRSVVRVSVKRPLAHGVSVEQDRIVLVSGGARSEIADIGGIGSLRGRHNAQNAACASAGA
CCEEEEEEEHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHCCCC
LALGVSRDILQQDLRSFPGLAHRMEQVGRKAHVLFVNDSKGTNADATEKALSSFDEIFWI
EEECCCHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEE
AGGKPKSGGIASLDAFFPRIRKAYLIGEAAQEFAATLEGKVAYEISGTLEAAVPAAARDA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCC
EGAGRAGAVVLLSPACASFDQFRNFEVRGDRFRELVQALPGVTPVV
CCCCCCCEEEEECCCHHCHHHHCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA