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The map label for this gene is lpsJ [H]

Identifier: 75675204

GI number: 75675204

Start: 1107156

End: 1107806

Strand: Direct

Name: lpsJ [H]

Synonym: Nwi_1011

Alternate gene names: 75675204

Gene position: 1107156-1107806 (Clockwise)

Preceding gene: 75675203

Following gene: 75675206

Centisome position: 32.54

GC content: 62.06

Gene sequence:

>651_bases
ATGGCCTGGACCCGCGAGCAGATGGCGGCGCGCGCCGCCAAGGAATTGCGCGACGGCTATTACGTCAATCTCGGAATCGG
TATCCCGACGCTGGTGTCGAACTTCATTCCCGACAATATCGACGTTCAGCTTCAGAGCGAGAACGGCATGCTCGGCATGG
GTCCCTTCCCTTATGAAGGCGAGGAGGATCCCGATCTTATCAACGCGGGCAAGCAGACCGTCACGGAATTGCCGACCACC
AGTTATTTCTCGTCGGCGGACTCGTTCGGCATGGTGCGCGGCGGTCATATCGACCTCTCGATCCTCGGGGCGATGCAGGT
GGCGCAGAATGGCGATCTCGCCAACTGGATGATCCCCGGCAAGATGGTCAAGGGAATGGGCGGCGCGATGGATCTCGTCG
CCGGCGTCAAGCGTGTCCTCGTCGTGATGGAGCATTCCGCCAAGGATGCGCCGAAGCTGCTCAAGCGATGCACGCTGCCG
CTGACCGGCGAACGGGTTGTCGATATGGTCGTGACCGATCTTGCGGTGTTCACCATCGACAAGCACGGCGACGGGGGCAT
GAGGCTGATCGAACTCGCCGGCGGCGTTACGCTCGATGAGGTCAGGGCCAGGACCGAAGCCGAATTCGGCGTCGCGCTGC
CGAACGCCTGA

Upstream 100 bases:

>100_bases
AGCACATCGAGTTCCGTAATACCCGTCCACGCCCGGCGGCGGCATGATCCGGAAAAGCACCAAGCTCCTTGCAATGATGA
CAAGGTGACAGGAGAGCCTC

Downstream 100 bases:

>100_bases
GGCGGTGCGCGACCGCCGCGCGACGGAGGGCATTGCGCTTATAGCAGCGCGGAGAAAGCGCGCCCGCCCCGAGGTCAGTC
CTGGGATGGCGCGCTCGCTT

Product: coenzyme A transferase

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNFIPDNIDVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPTT
SYFSSADSFGMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVLVVMEHSAKDAPKLLKRCTLP
LTGERVVDMVVTDLAVFTIDKHGDGGMRLIELAGGVTLDEVRARTEAEFGVALPNA

Sequences:

>Translated_216_residues
MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNFIPDNIDVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPTT
SYFSSADSFGMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVLVVMEHSAKDAPKLLKRCTLP
LTGERVVDMVVTDLAVFTIDKHGDGGMRLIELAGGVTLDEVRARTEAEFGVALPNA
>Mature_215_residues
AWTREQMAARAAKELRDGYYVNLGIGIPTLVSNFIPDNIDVQLQSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPTTS
YFSSADSFGMVRGGHIDLSILGAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVLVVMEHSAKDAPKLLKRCTLPL
TGERVVDMVVTDLAVFTIDKHGDGGMRLIELAGGVTLDEVRARTEAEFGVALPNA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=210, Percent_Identity=60.952380952381, Blast_Score=258, Evalue=2e-69,
Organism=Homo sapiens, GI11545841, Length=213, Percent_Identity=58.2159624413146, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI1788552, Length=209, Percent_Identity=54.066985645933, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17531517, Length=211, Percent_Identity=54.0284360189574, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21358325, Length=213, Percent_Identity=59.1549295774648, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24655794, Length=213, Percent_Identity=59.1549295774648, Blast_Score=256, Evalue=8e-69,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23168; Mature: 23037

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNFIPDNIDVQLQSENGMLGMGPFPYEG
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
EEDPDLINAGKQTVTELPTTSYFSSADSFGMVRGGHIDLSILGAMQVAQNGDLANWMIPG
CCCCHHHHCCHHHHHHCCCCHHHCCCCCCCCEECCCEEEEHHHHHHHHCCCCCCEEECCH
KMVKGMGGAMDLVAGVKRVLVVMEHSAKDAPKLLKRCTLPLTGERVVDMVVTDLAVFTID
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEE
KHGDGGMRLIELAGGVTLDEVRARTEAEFGVALPNA
ECCCCCEEEEEECCCCCHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
AWTREQMAARAAKELRDGYYVNLGIGIPTLVSNFIPDNIDVQLQSENGMLGMGPFPYEG
CCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCC
EEDPDLINAGKQTVTELPTTSYFSSADSFGMVRGGHIDLSILGAMQVAQNGDLANWMIPG
CCCCHHHHCCHHHHHHCCCCHHHCCCCCCCCEECCCEEEEHHHHHHHHCCCCCCEEECCH
KMVKGMGGAMDLVAGVKRVLVVMEHSAKDAPKLLKRCTLPLTGERVVDMVVTDLAVFTID
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEE
KHGDGGMRLIELAGGVTLDEVRARTEAEFGVALPNA
ECCCCCEEEEEECCCCCHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]