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The map label for this gene is ampH [H]

Identifier: 75674850

GI number: 75674850

Start: 732820

End: 734415

Strand: Direct

Name: ampH [H]

Synonym: Nwi_0653

Alternate gene names: 75674850

Gene position: 732820-734415 (Clockwise)

Preceding gene: 75674849

Following gene: 75674853

Centisome position: 21.54

GC content: 60.96

Gene sequence:

>1596_bases
ATGTTCTTGCAGAAATCTGTTGTCGCATTTCGCCCGAGGGCAATCCTCGCAAAGCTGGTCGCCTCGTCCTTGGTCTGGGC
TTGTGCGGCGATACCGGGTTTTGCGGAGACATCGCCTCCCCAGACCTCAGGCGTACCCATTGCCGACGGTCAGATCGACG
AGGCCGTGGCGGGTCTCGACGCGATCGCCACCGGCGTCATGAAAAAGACCGGCATTCCGGGCATGGCGGTTGCGGTGGTG
CGTGATGGCAAGATCGCCTACGCGAAGGGTTTCGGCGTCCGGAAAGTTGGCGAGAACCAGCCGATTGACGCGAACACTGT
TTTTCAGATCGCATCGCTTTCAAAATCTATCGCGGCCACCGTCGTGGCTCGCCAGGTCGGCGCGGACGTCGTCAAGTGGA
ACACGCCTGTCCAGGCGCACTTGCCCTGGTTCAGGTTGATGGACCCGTGGGTGACGCAGCATGTCACGATCGCCGATTTG
TTCAGCCACCGTTCGGGGTTGCCGGACCACGCCGGCGACGATCTAGAGGATCTCGGCTATGGTCGCCGAGCTGTGCTGGA
GCGCCTGCGCCTGCTGCCGCTTCACGGTTTCCGGGACGTCTATGCCTACACGAATTTCGGGCTGACCGCGGCGGCGGAAG
CCGTCGCCGCCGCATCAGGCAAGGACTGGGAAAGCCTGTCTGAAGAGGTTCTTTATAAACCGCTCGGGATGAGCGCGACC
AGTTCGCGCTTTGCTGATTTTGAAAAGCAGGCCAATCGCGCCATTGGACATGTAAGGGGCGGCGACGGCTTCCAGCCAAA
GTATCAACGCCGGCCCGATGCGCAGTCACCCGCAGGCGGTGTCAGTTCGTCGGCAAACGATCTTGCCCGATGGCTGGCGT
TGGTGTTGCGGAACGGCGAATTCGAAGGCCGGCAGGTCATTCCAGCGGATGCTCTGCTTCCAGCCATGACCGCCGAGGTC
GTTTCGATTCATTCCCCTTCAGCCGATGCGAGAGCCAGCCTGTACGGATATGGATTTGGCGTCGGAGTCACGCCTGCGGG
ACGAACAATGATCAGTCATTCCGGGGCCTTCGCGCTGGGTGCAGGAACCCATTACACGATCATCCCTTCGGCCGGGATCG
GGATCGTCGTGCTGACCAACGCGGAACCGGTCGGCGCGGCGGAGGCGGTCGGGGCCTCGTTCATGGATCTTGTTCAGTTC
GGGAAGATCACGCGGGACTGGCTTGCTGCCTATAGCGGGGTGATGGCGCAGCTTACGGCGCCGGCAGGCGATCTCGTCGG
CAAGTCGCCGCCCGCTAAACCAGCGCCGGCTGCCAGTTCTACATCCTATGTTGGCCGCTACGGCAGTCCCTATTTCGGAG
ATGCCGAGATCGTCGATGCAAACGGTCATATGGAGCTGAAGATCGGCCGTTTGAGCCGATCGTACACGTTGACGCACTGG
GATGGCGACGTGTTTTCGATCAAACCTTCCAGCGAGAACCAAACCGACGGTTCGGTGTCGTCGGTTACTTTCAAGCGAAC
TGGCGACGAGCCGGCCAGCGAACTGACTATCGAGTTTCTCAACGACAATGGGCTCGGCCAGTTCATGAGGCGCTGA

Upstream 100 bases:

>100_bases
TCGCCATCGACACGTTTCGCGGCATCGTCTGCGCCGGCCGGGACCGCCCCGTCGCCGTGGGGACGTTATGCATCTAAAAG
CGGGTAGCTGAAGGCCTCGC

Downstream 100 bases:

>100_bases
AAACATTCTCCCGGAGGAATTCGGTCGGCCGCCAGGCGCCAACACCCTCTATCGGGAGAGGATGGCAACAGGAATTGAAT
GCGTTCTCCAGATAATGGAG

Product: beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MFLQKSVVAFRPRAILAKLVASSLVWACAAIPGFAETSPPQTSGVPIADGQIDEAVAGLDAIATGVMKKTGIPGMAVAVV
RDGKIAYAKGFGVRKVGENQPIDANTVFQIASLSKSIAATVVARQVGADVVKWNTPVQAHLPWFRLMDPWVTQHVTIADL
FSHRSGLPDHAGDDLEDLGYGRRAVLERLRLLPLHGFRDVYAYTNFGLTAAAEAVAAASGKDWESLSEEVLYKPLGMSAT
SSRFADFEKQANRAIGHVRGGDGFQPKYQRRPDAQSPAGGVSSSANDLARWLALVLRNGEFEGRQVIPADALLPAMTAEV
VSIHSPSADARASLYGYGFGVGVTPAGRTMISHSGAFALGAGTHYTIIPSAGIGIVVLTNAEPVGAAEAVGASFMDLVQF
GKITRDWLAAYSGVMAQLTAPAGDLVGKSPPAKPAPAASSTSYVGRYGSPYFGDAEIVDANGHMELKIGRLSRSYTLTHW
DGDVFSIKPSSENQTDGSVSSVTFKRTGDEPASELTIEFLNDNGLGQFMRR

Sequences:

>Translated_531_residues
MFLQKSVVAFRPRAILAKLVASSLVWACAAIPGFAETSPPQTSGVPIADGQIDEAVAGLDAIATGVMKKTGIPGMAVAVV
RDGKIAYAKGFGVRKVGENQPIDANTVFQIASLSKSIAATVVARQVGADVVKWNTPVQAHLPWFRLMDPWVTQHVTIADL
FSHRSGLPDHAGDDLEDLGYGRRAVLERLRLLPLHGFRDVYAYTNFGLTAAAEAVAAASGKDWESLSEEVLYKPLGMSAT
SSRFADFEKQANRAIGHVRGGDGFQPKYQRRPDAQSPAGGVSSSANDLARWLALVLRNGEFEGRQVIPADALLPAMTAEV
VSIHSPSADARASLYGYGFGVGVTPAGRTMISHSGAFALGAGTHYTIIPSAGIGIVVLTNAEPVGAAEAVGASFMDLVQF
GKITRDWLAAYSGVMAQLTAPAGDLVGKSPPAKPAPAASSTSYVGRYGSPYFGDAEIVDANGHMELKIGRLSRSYTLTHW
DGDVFSIKPSSENQTDGSVSSVTFKRTGDEPASELTIEFLNDNGLGQFMRR
>Mature_531_residues
MFLQKSVVAFRPRAILAKLVASSLVWACAAIPGFAETSPPQTSGVPIADGQIDEAVAGLDAIATGVMKKTGIPGMAVAVV
RDGKIAYAKGFGVRKVGENQPIDANTVFQIASLSKSIAATVVARQVGADVVKWNTPVQAHLPWFRLMDPWVTQHVTIADL
FSHRSGLPDHAGDDLEDLGYGRRAVLERLRLLPLHGFRDVYAYTNFGLTAAAEAVAAASGKDWESLSEEVLYKPLGMSAT
SSRFADFEKQANRAIGHVRGGDGFQPKYQRRPDAQSPAGGVSSSANDLARWLALVLRNGEFEGRQVIPADALLPAMTAEV
VSIHSPSADARASLYGYGFGVGVTPAGRTMISHSGAFALGAGTHYTIIPSAGIGIVVLTNAEPVGAAEAVGASFMDLVQF
GKITRDWLAAYSGVMAQLTAPAGDLVGKSPPAKPAPAASSTSYVGRYGSPYFGDAEIVDANGHMELKIGRLSRSYTLTHW
DGDVFSIKPSSENQTDGSVSSVTFKRTGDEPASELTIEFLNDNGLGQFMRR

Specific function: Binds penicillin and contributes to the normal morphology [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1786574, Length=350, Percent_Identity=24, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533026, Length=355, Percent_Identity=23.943661971831, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 56122; Mature: 56122

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLQKSVVAFRPRAILAKLVASSLVWACAAIPGFAETSPPQTSGVPIADGQIDEAVAGLD
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AIATGVMKKTGIPGMAVAVVRDGKIAYAKGFGVRKVGENQPIDANTVFQIASLSKSIAAT
HHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
VVARQVGADVVKWNTPVQAHLPWFRLMDPWVTQHVTIADLFSHRSGLPDHAGDDLEDLGY
HHHHHHCCCEEECCCCCHHCCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC
GRRAVLERLRLLPLHGFRDVYAYTNFGLTAAAEAVAAASGKDWESLSEEVLYKPLGMSAT
CHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
SSRFADFEKQANRAIGHVRGGDGFQPKYQRRPDAQSPAGGVSSSANDLARWLALVLRNGE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FEGRQVIPADALLPAMTAEVVSIHSPSADARASLYGYGFGVGVTPAGRTMISHSGAFALG
CCCCEECCHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCEEEC
AGTHYTIIPSAGIGIVVLTNAEPVGAAEAVGASFMDLVQFGKITRDWLAAYSGVMAQLTA
CCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAGDLVGKSPPAKPAPAASSTSYVGRYGSPYFGDAEIVDANGHMELKIGRLSRSYTLTHW
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCEEEEEEE
DGDVFSIKPSSENQTDGSVSSVTFKRTGDEPASELTIEFLNDNGLGQFMRR
CCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHEEEEEECCCCHHHHHCC
>Mature Secondary Structure
MFLQKSVVAFRPRAILAKLVASSLVWACAAIPGFAETSPPQTSGVPIADGQIDEAVAGLD
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AIATGVMKKTGIPGMAVAVVRDGKIAYAKGFGVRKVGENQPIDANTVFQIASLSKSIAAT
HHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
VVARQVGADVVKWNTPVQAHLPWFRLMDPWVTQHVTIADLFSHRSGLPDHAGDDLEDLGY
HHHHHHCCCEEECCCCCHHCCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC
GRRAVLERLRLLPLHGFRDVYAYTNFGLTAAAEAVAAASGKDWESLSEEVLYKPLGMSAT
CHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
SSRFADFEKQANRAIGHVRGGDGFQPKYQRRPDAQSPAGGVSSSANDLARWLALVLRNGE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FEGRQVIPADALLPAMTAEVVSIHSPSADARASLYGYGFGVGVTPAGRTMISHSGAFALG
CCCCEECCHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCEEEC
AGTHYTIIPSAGIGIVVLTNAEPVGAAEAVGASFMDLVQFGKITRDWLAAYSGVMAQLTA
CCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAGDLVGKSPPAKPAPAASSTSYVGRYGSPYFGDAEIVDANGHMELKIGRLSRSYTLTHW
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCEEEEEEE
DGDVFSIKPSSENQTDGSVSSVTFKRTGDEPASELTIEFLNDNGLGQFMRR
CCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHEEEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]