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The map label for this gene is clpB [H]

Identifier: 75674787

GI number: 75674787

Start: 663965

End: 666640

Strand: Direct

Name: clpB [H]

Synonym: Nwi_0589

Alternate gene names: 75674787

Gene position: 663965-666640 (Clockwise)

Preceding gene: 75674785

Following gene: 75674788

Centisome position: 19.52

GC content: 64.46

Gene sequence:

>2676_bases
GTGCTTTCGGGGCAGGGGCGGCAGGCCGGGGAGACTCACATGAACATTGAAAAATATACCGAGCGGGTGCGCGGGTTCAT
CCAGTCGGCGCAATCGCTGGCGATGCGTGAAGGCCATCAGCAGTTTACACCGCTCCATATTCTGAAGGTGCTGCTTGATG
ACAGCGAAGGTCTGGCGGGAAGCCTGATCGACCGCGCCGGCGGCAATTCGCGCGCGATCCTGAAGGCGACGGAAGCCGCT
CTCGACAAGATGCCGAAGGTGTCAGGCGCCGGGGCGGGCCAGATTTATCTCGCGCCGGCGACGGCGCGCGCGTTCGATGC
CGCGGAGCAGGCGGCCGGGAAGGCCGGCGACAGCTTCGTTACCGTCGAACGGCTGTTGCAGTCCCTGTCGCTCGACGCGG
ATAGCGACGCGTTCAAATTGCTGAAGGACGGCGGGGTCACGCCGCAGAACCTCAACGCGGCGATCAACGCGCTGCGCAAA
GGGCGGACCGCGGATAGCGCCACGGCGGAAAACGCCTATGACGCGTTGAAGAAATACGCCCGCGATCTGACCCAGGCGGC
GCGCGAGGGCAAGCTCGATCCGGTGATCGGCCGCGACGAGGAGATCCGCCGCACCATCCAGGTGCTGTCGCGGCGCACCA
AGAACAATCCGGTGCTGATCGGCGAGCCCGGCGTCGGCAAGACCGCGATCGCGGAGGGACTGGCGCTGCGTATCGTCAAC
GGCGATGTGCCGGAGAGCCTCAAGGACAAGAAGCTGCTCGCGCTCGACCTCGGGTCGATGATCGCGGGCGCGAAGTACCG
CGGCGAGTTCGAGGAGCGGCTCAAGGCGGTGCTGCAGGAGGTGACCTCCGCCGATGGCGGCATCGTCCTGTTCATCGATG
AGATGCACACGCTGATCGGCGCCGGCAAGGCTGATGGCGCCATGGACGCCTCCAACCTGCTGAAGCCTGCGCTGGCGCGC
GGCGAACTGCATTGCGTCGGAGCGACCACGCTCGACGAGTATCGCAAGCATGTCGAGAAGGATGCGGCGCTGGCCAGGCG
CTTTCAGCCGATCTTCGTGTCCGAGCCGACGGTCGAGGATACGGTCTCGATCCTGCGCGGTCTCAAGGACAAGTACGAGC
AACATCACGGCGTCCGCATCGCCGATAGCGCGCTGGTCGCGGCCGCCACGCTCTCTCACCGTTATATCACCGATCGTTTC
CTGCCTGACAAGGCGATCGATCTGGTGGATGAGGCGGCGGCGCGGCTCAAGATGCAGGTCGACTCCAAGCCGGAAGAACT
CGATTCGCTCGACCGCGAGATCGTGCGGCTGAAGATCGAGCAGGAGGCGCTGAAGAAGGAGAGCGACGCCGGCTCCAGAA
CGCGTCTTGAGAACCTCGAGAAGGAACTGGCCGATCTGGAGAAGCGGTCGGCGGACCTGACCTCGCGCTGGAACGCCGAG
AAGAACAAGCTGTCTGATGCCGCGAGGCTCAAGAGCGAACTCGATCAGGCGCGGATCGAACTCGCCAACGCGCAACGGCG
CGGCGAATATCAGATAGCCGGCGAACTGGCCTATGGGCGGATTCCCGAACTCGAAAAGCGTCTGCAGAGTATCGAGGCCG
GCGAAAGCAATACGATGATGAATGAAACCGTGACGGCGGACAGCATCGCTCAGGTGGTGTCACGCTGGACCGGCGTTCCC
GTCGACAAGATGCTCGAGGGCGAGAAGGAGAAGCTGCTGCGCATGGAGGAAAGCTTGGCGGCGCGCGTCGTGGGGCAGGG
TGAAGCCGTGCGGGCGGTGTCAACGGCGGTGCGGCGTGCCCGCGCCGGTTTGCAGGACCCCAACCGTCCGATGGGTTCGT
TCATGTTCCTGGGACCGACCGGCGTCGGCAAAACCGAACTGACCAAGGCGCTCGCCGCCTATCTGTTCGACGATGAGACG
GCGATGGTCCGCATCGACATGTCGGAATACATGGAGAAGCACTCGGTGGCGCGGCTGATCGGCGCGCCTCCCGGCTATGT
CGGTTACGACGAGGGCGGCGCGCTGACCGAGGCGGTGCGGCGTCGTCCCTATCAGGTCGTGCTGTTCGACGAGATCGAGA
AGGCGCATCCGGACGTGTTCAACGTGCTGTTGCAGGTGCTCGACGACGGCCGCCTGACCGACGGACAGGGCCGCACCGTG
GATTTCCGCAACACGCTGATCATCATGACATCGAACCTCGGTTCGGAATTCCTGGTCAATCAACCCGAAGGGGAAGACAC
CGGCGCGGTGCGCGAGCAGGTGATGGAGATGGTGCGCGGGCATTTCCGTCCCGAATTCCTCAACCGCGTCGACGAGATCA
TCCTGTTTCATCGGTTGCAGAAGAGCGAGATGGGCCGGATCGTGGAAATTCAGTTCTCGAGGCTGCAAAAGCTGCTGGAG
GATCGCAAGATCGTGCTCGATCTCGACTCCGCGGCGCGCGACCGGCTGGCCGAGAAAGGCTGGGATCCCGCCTACGGCGC
GCGGCCGCTCAAGCGCGTGATCCAGCGCAGCGTGCAGGATCCGCTGGCTGAAATGATCCTGGCGGGCGCCGTCCGCGACG
GCGACCGGGTCGTGATTTCAGCCAGGGACGGCGTACTCACATTCAATGGCGAGGCGCCGCCGGCGGCTGATATCGAGCCA
TTCTCGCGGCCGGTGCCCAGGCGCAAGCTGCACTGA

Upstream 100 bases:

>100_bases
CTACATCTGCCGCAGGCCGAATTGAACGATCCGGCGAGCCGGTCGCTGCCGGTTGAGGAATGTCAATGCGGCCGGCGTAT
GCAACGAGGATGCTGCTTCT

Downstream 100 bases:

>100_bases
GGCGCGCCTGTTCAAGCCTTCCTGCGCCCGCAGGAGGCTTGACGCCGCCGTCACGCGAATCTCATGAATGCTCCCAGTGC
AATTCTAAAAAAGGCCGATA

Product: AAA ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 891; Mature: 891

Protein sequence:

>891_residues
MLSGQGRQAGETHMNIEKYTERVRGFIQSAQSLAMREGHQQFTPLHILKVLLDDSEGLAGSLIDRAGGNSRAILKATEAA
LDKMPKVSGAGAGQIYLAPATARAFDAAEQAAGKAGDSFVTVERLLQSLSLDADSDAFKLLKDGGVTPQNLNAAINALRK
GRTADSATAENAYDALKKYARDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVN
GDVPESLKDKKLLALDLGSMIAGAKYRGEFEERLKAVLQEVTSADGGIVLFIDEMHTLIGAGKADGAMDASNLLKPALAR
GELHCVGATTLDEYRKHVEKDAALARRFQPIFVSEPTVEDTVSILRGLKDKYEQHHGVRIADSALVAAATLSHRYITDRF
LPDKAIDLVDEAAARLKMQVDSKPEELDSLDREIVRLKIEQEALKKESDAGSRTRLENLEKELADLEKRSADLTSRWNAE
KNKLSDAARLKSELDQARIELANAQRRGEYQIAGELAYGRIPELEKRLQSIEAGESNTMMNETVTADSIAQVVSRWTGVP
VDKMLEGEKEKLLRMEESLAARVVGQGEAVRAVSTAVRRARAGLQDPNRPMGSFMFLGPTGVGKTELTKALAAYLFDDET
AMVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTV
DFRNTLIIMTSNLGSEFLVNQPEGEDTGAVREQVMEMVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLE
DRKIVLDLDSAARDRLAEKGWDPAYGARPLKRVIQRSVQDPLAEMILAGAVRDGDRVVISARDGVLTFNGEAPPAADIEP
FSRPVPRRKLH

Sequences:

>Translated_891_residues
MLSGQGRQAGETHMNIEKYTERVRGFIQSAQSLAMREGHQQFTPLHILKVLLDDSEGLAGSLIDRAGGNSRAILKATEAA
LDKMPKVSGAGAGQIYLAPATARAFDAAEQAAGKAGDSFVTVERLLQSLSLDADSDAFKLLKDGGVTPQNLNAAINALRK
GRTADSATAENAYDALKKYARDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVN
GDVPESLKDKKLLALDLGSMIAGAKYRGEFEERLKAVLQEVTSADGGIVLFIDEMHTLIGAGKADGAMDASNLLKPALAR
GELHCVGATTLDEYRKHVEKDAALARRFQPIFVSEPTVEDTVSILRGLKDKYEQHHGVRIADSALVAAATLSHRYITDRF
LPDKAIDLVDEAAARLKMQVDSKPEELDSLDREIVRLKIEQEALKKESDAGSRTRLENLEKELADLEKRSADLTSRWNAE
KNKLSDAARLKSELDQARIELANAQRRGEYQIAGELAYGRIPELEKRLQSIEAGESNTMMNETVTADSIAQVVSRWTGVP
VDKMLEGEKEKLLRMEESLAARVVGQGEAVRAVSTAVRRARAGLQDPNRPMGSFMFLGPTGVGKTELTKALAAYLFDDET
AMVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTV
DFRNTLIIMTSNLGSEFLVNQPEGEDTGAVREQVMEMVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLE
DRKIVLDLDSAARDRLAEKGWDPAYGARPLKRVIQRSVQDPLAEMILAGAVRDGDRVVISARDGVLTFNGEAPPAADIEP
FSRPVPRRKLH
>Mature_891_residues
MLSGQGRQAGETHMNIEKYTERVRGFIQSAQSLAMREGHQQFTPLHILKVLLDDSEGLAGSLIDRAGGNSRAILKATEAA
LDKMPKVSGAGAGQIYLAPATARAFDAAEQAAGKAGDSFVTVERLLQSLSLDADSDAFKLLKDGGVTPQNLNAAINALRK
GRTADSATAENAYDALKKYARDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVN
GDVPESLKDKKLLALDLGSMIAGAKYRGEFEERLKAVLQEVTSADGGIVLFIDEMHTLIGAGKADGAMDASNLLKPALAR
GELHCVGATTLDEYRKHVEKDAALARRFQPIFVSEPTVEDTVSILRGLKDKYEQHHGVRIADSALVAAATLSHRYITDRF
LPDKAIDLVDEAAARLKMQVDSKPEELDSLDREIVRLKIEQEALKKESDAGSRTRLENLEKELADLEKRSADLTSRWNAE
KNKLSDAARLKSELDQARIELANAQRRGEYQIAGELAYGRIPELEKRLQSIEAGESNTMMNETVTADSIAQVVSRWTGVP
VDKMLEGEKEKLLRMEESLAARVVGQGEAVRAVSTAVRRARAGLQDPNRPMGSFMFLGPTGVGKTELTKALAAYLFDDET
AMVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTV
DFRNTLIIMTSNLGSEFLVNQPEGEDTGAVREQVMEMVRGHFRPEFLNRVDEIILFHRLQKSEMGRIVEIQFSRLQKLLE
DRKIVLDLDSAARDRLAEKGWDPAYGARPLKRVIQRSVQDPLAEMILAGAVRDGDRVVISARDGVLTFNGEAPPAADIEP
FSRPVPRRKLH

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=337, Percent_Identity=36.4985163204748, Blast_Score=207, Evalue=3e-53,
Organism=Escherichia coli, GI1788943, Length=844, Percent_Identity=57.8199052132701, Blast_Score=991, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=323, Percent_Identity=44.8916408668731, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=700, Percent_Identity=57, Blast_Score=811, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=866, Percent_Identity=44.3418013856813, Blast_Score=700, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 97968; Mature: 97968

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGQGRQAGETHMNIEKYTERVRGFIQSAQSLAMREGHQQFTPLHILKVLLDDSEGLAG
CCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHCCCCCCHH
SLIDRAGGNSRAILKATEAALDKMPKVSGAGAGQIYLAPATARAFDAAEQAAGKAGDSFV
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHH
TVERLLQSLSLDADSDAFKLLKDGGVTPQNLNAAINALRKGRTADSATAENAYDALKKYA
HHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVN
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEEC
GDVPESLKDKKLLALDLGSMIAGAKYRGEFEERLKAVLQEVTSADGGIVLFIDEMHTLIG
CCCCCCCCCCEEHEEEHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHC
AGKADGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARRFQPIFVSEPTVED
CCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHH
TVSILRGLKDKYEQHHGVRIADSALVAAATLSHRYITDRFLPDKAIDLVDEAAARLKMQV
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
DSKPEELDSLDREIVRLKIEQEALKKESDAGSRTRLENLEKELADLEKRSADLTSRWNAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KNKLSDAARLKSELDQARIELANAQRRGEYQIAGELAYGRIPELEKRLQSIEAGESNTMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCH
NETVTADSIAQVVSRWTGVPVDKMLEGEKEKLLRMEESLAARVVGQGEAVRAVSTAVRRA
HHHCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RAGLQDPNRPMGSFMFLGPTGVGKTELTKALAAYLFDDETAMVRIDMSEYMEKHSVARLI
HCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHH
GAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTV
CCCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEE
DFRNTLIIMTSNLGSEFLVNQPEGEDTGAVREQVMEMVRGHFRPEFLNRVDEIILFHRLQ
EECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KSEMGRIVEIQFSRLQKLLEDRKIVLDLDSAARDRLAEKGWDPAYGARPLKRVIQRSVQD
HHHCCCEEEHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
PLAEMILAGAVRDGDRVVISARDGVLTFNGEAPPAADIEPFSRPVPRRKLH
HHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSGQGRQAGETHMNIEKYTERVRGFIQSAQSLAMREGHQQFTPLHILKVLLDDSEGLAG
CCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHCCCCCCHH
SLIDRAGGNSRAILKATEAALDKMPKVSGAGAGQIYLAPATARAFDAAEQAAGKAGDSFV
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHH
TVERLLQSLSLDADSDAFKLLKDGGVTPQNLNAAINALRKGRTADSATAENAYDALKKYA
HHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVN
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEEC
GDVPESLKDKKLLALDLGSMIAGAKYRGEFEERLKAVLQEVTSADGGIVLFIDEMHTLIG
CCCCCCCCCCEEHEEEHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHC
AGKADGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARRFQPIFVSEPTVED
CCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHH
TVSILRGLKDKYEQHHGVRIADSALVAAATLSHRYITDRFLPDKAIDLVDEAAARLKMQV
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
DSKPEELDSLDREIVRLKIEQEALKKESDAGSRTRLENLEKELADLEKRSADLTSRWNAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KNKLSDAARLKSELDQARIELANAQRRGEYQIAGELAYGRIPELEKRLQSIEAGESNTMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCH
NETVTADSIAQVVSRWTGVPVDKMLEGEKEKLLRMEESLAARVVGQGEAVRAVSTAVRRA
HHHCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RAGLQDPNRPMGSFMFLGPTGVGKTELTKALAAYLFDDETAMVRIDMSEYMEKHSVARLI
HCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHH
GAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTV
CCCCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEE
DFRNTLIIMTSNLGSEFLVNQPEGEDTGAVREQVMEMVRGHFRPEFLNRVDEIILFHRLQ
EECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KSEMGRIVEIQFSRLQKLLEDRKIVLDLDSAARDRLAEKGWDPAYGARPLKRVIQRSVQD
HHHCCCEEEHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
PLAEMILAGAVRDGDRVVISARDGVLTFNGEAPPAADIEPFSRPVPRRKLH
HHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA