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The map label for this gene is cheB

Identifier: 75674721

GI number: 75674721

Start: 580784

End: 582061

Strand: Direct

Name: cheB

Synonym: Nwi_0523

Alternate gene names: 75674721

Gene position: 580784-582061 (Clockwise)

Preceding gene: 75674720

Following gene: 75674722

Centisome position: 17.07

GC content: 64.32

Gene sequence:

>1278_bases
ATGGGCATGAGTACTGCGGTGGCAAGTCCGGTGGCTCTGGATTCGGGAAAGCTGGAGCCGCTACGGGTCATGGTGGTCGA
TGATTCCGTCGTTATCCGCGGGCTGATTTCCAGATGGATCGAGGCCGAGCCCGACATGATGGTCGCGGCCTCGCTTCGCA
CCGGCCGCGACGCCGTCAGCCAGGTCGAGCGCGCCGATCCGGACGTCGTGGTCCTCGACATCGAAATGCCCGAACTCGAC
GGCATTTCCGCGCTGCCGCAACTGCTGGCCAAAAAGCGCAACCTCATCGTCATCATGGCCTCGACGCTGACCCGCCGCAA
CGCGGAGATCAGTTTCAAGGCGCTGTCGCTCGGCGCGTCCGATTACATTCCGAAGCCCGAGAGCACGCGCGAGGTCGCCG
CGGCTGATATCTTCAGGCATGACCTGATGCAGAAGATCCGGCATCTCGCCGCGAAACGCCGTCGTCCGGCGACAGTCGCT
TCGCCGCCGCCAGATCACGATGATTATGGATCGAACGCATCCACAATCATGAACGCGGTCGATTCCAATATTTCAGAGCG
GGATGCGGGCGGAAAACCGCGACGCACTTTTCCTCATCCCGCTCTGGTCCAGCGCGAACAGCAACCGCGTTCTGCTCAAG
CCGCTCGCGCGATGAGCCGGCCCCAGCCAACGCTGCGTTCCTTCAGCGCGCACCTCCCGCGTGCGCTGCTGATCGGTTCG
TCCACGGGCGGGCCGCAGGCGCTGATGACGCTGGTGGCCGGAATAGGACCGGTGATCGACCGCTGTCCGGTGCTGATCAC
CCAGCACATGCCCCCGACGTTCACCACCATTCTCGCCGAGCATCTGGCGCGGGCCGCGGGGCGTCCGGCGCATGAGGGCG
TGGATCAGGAGATCGTGAAGCAGGGCCATATCTATCTCGCGCCCGGCGGCCGTCACATGCGGGTGGCGAGAAAAGGAGCA
GACGCGGTCATCGTTCTGGATAACGGCCCGGCGGTGAATTTCTGCAAGCCGGCCGTCGATCCTTTGTTCATGTCGGCGAT
CGACGTCTGGCAGGGCGGCGCTCTGGCCGTGATCCTGACCGGAATGGGGTCGGACGGAATGCGTGGAGGCACGCAGATTG
TCGCCGCGGGCGGCAGCATCATCGCCCAGGACGAAGCAAGCAGCGTGGTGTGGGGGATGCCGGGCGCCGCCGTTCAGGCG
GGAATTTGCGCGGCCGTTCTGCCGCTTCAACAAATTGCTCCGAAGCTGGTTCGGCTATTCGCGGGAGACGGTTTGTGA

Upstream 100 bases:

>100_bases
GAAGCCGTTCGACAAGGACATCGTCACGGCGAAATTTCAGGAAATCGGCCTGATCTGAATCTCGTCGCGATCCGGCCGGA
TATTTTTCATCTTGCGCGTA

Downstream 100 bases:

>100_bases
CACCGCTTGATTTCGAGTACTTGCGCAAGTTGCTGAAGGAACGGTCCGGCCTCGACTTGACCGCCGAGAAGCAATATCTG
GTCGAAAGTCGGCTGACTCC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MGMSTAVASPVALDSGKLEPLRVMVVDDSVVIRGLISRWIEAEPDMMVAASLRTGRDAVSQVERADPDVVVLDIEMPELD
GISALPQLLAKKRNLIVIMASTLTRRNAEISFKALSLGASDYIPKPESTREVAAADIFRHDLMQKIRHLAAKRRRPATVA
SPPPDHDDYGSNASTIMNAVDSNISERDAGGKPRRTFPHPALVQREQQPRSAQAARAMSRPQPTLRSFSAHLPRALLIGS
STGGPQALMTLVAGIGPVIDRCPVLITQHMPPTFTTILAEHLARAAGRPAHEGVDQEIVKQGHIYLAPGGRHMRVARKGA
DAVIVLDNGPAVNFCKPAVDPLFMSAIDVWQGGALAVILTGMGSDGMRGGTQIVAAGGSIIAQDEASSVVWGMPGAAVQA
GICAAVLPLQQIAPKLVRLFAGDGL

Sequences:

>Translated_425_residues
MGMSTAVASPVALDSGKLEPLRVMVVDDSVVIRGLISRWIEAEPDMMVAASLRTGRDAVSQVERADPDVVVLDIEMPELD
GISALPQLLAKKRNLIVIMASTLTRRNAEISFKALSLGASDYIPKPESTREVAAADIFRHDLMQKIRHLAAKRRRPATVA
SPPPDHDDYGSNASTIMNAVDSNISERDAGGKPRRTFPHPALVQREQQPRSAQAARAMSRPQPTLRSFSAHLPRALLIGS
STGGPQALMTLVAGIGPVIDRCPVLITQHMPPTFTTILAEHLARAAGRPAHEGVDQEIVKQGHIYLAPGGRHMRVARKGA
DAVIVLDNGPAVNFCKPAVDPLFMSAIDVWQGGALAVILTGMGSDGMRGGTQIVAAGGSIIAQDEASSVVWGMPGAAVQA
GICAAVLPLQQIAPKLVRLFAGDGL
>Mature_424_residues
GMSTAVASPVALDSGKLEPLRVMVVDDSVVIRGLISRWIEAEPDMMVAASLRTGRDAVSQVERADPDVVVLDIEMPELDG
ISALPQLLAKKRNLIVIMASTLTRRNAEISFKALSLGASDYIPKPESTREVAAADIFRHDLMQKIRHLAAKRRRPATVAS
PPPDHDDYGSNASTIMNAVDSNISERDAGGKPRRTFPHPALVQREQQPRSAQAARAMSRPQPTLRSFSAHLPRALLIGSS
TGGPQALMTLVAGIGPVIDRCPVLITQHMPPTFTTILAEHLARAAGRPAHEGVDQEIVKQGHIYLAPGGRHMRVARKGAD
AVIVLDNGPAVNFCKPAVDPLFMSAIDVWQGGALAVILTGMGSDGMRGGTQIVAAGGSIIAQDEASSVVWGMPGAAVQAG
ICAAVLPLQQIAPKLVRLFAGDGL

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=194, Percent_Identity=41.2371134020619, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_NITWN (Q3SVA1)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317142.1
- ProteinModelPortal:   Q3SVA1
- STRING:   Q3SVA1
- GeneID:   3675524
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0523
- NMPDR:   fig|323098.3.peg.218
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   IIIMAST
- PhylomeDB:   Q3SVA1
- ProtClustDB:   PRK00742
- BioCyc:   NWIN323098:NWI_0523-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 45008; Mature: 44876

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 241-241 ACT_SITE 269-269 ACT_SITE 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMSTAVASPVALDSGKLEPLRVMVVDDSVVIRGLISRWIEAEPDMMVAASLRTGRDAVS
CCCCHHHCCCEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHH
QVERADPDVVVLDIEMPELDGISALPQLLAKKRNLIVIMASTLTRRNAEISFKALSLGAS
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCC
DYIPKPESTREVAAADIFRHDLMQKIRHLAAKRRRPATVASPPPDHDDYGSNASTIMNAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DSNISERDAGGKPRRTFPHPALVQREQQPRSAQAARAMSRPQPTLRSFSAHLPRALLIGS
HCCCHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEC
STGGPQALMTLVAGIGPVIDRCPVLITQHMPPTFTTILAEHLARAAGRPAHEGVDQEIVK
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QGHIYLAPGGRHMRVARKGADAVIVLDNGPAVNFCKPAVDPLFMSAIDVWQGGALAVILT
CCCEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
GMGSDGMRGGTQIVAAGGSIIAQDEASSVVWGMPGAAVQAGICAAVLPLQQIAPKLVRLF
ECCCCCCCCCCEEEEECCCEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGDGL
HCCCC
>Mature Secondary Structure 
GMSTAVASPVALDSGKLEPLRVMVVDDSVVIRGLISRWIEAEPDMMVAASLRTGRDAVS
CCCHHHCCCEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHH
QVERADPDVVVLDIEMPELDGISALPQLLAKKRNLIVIMASTLTRRNAEISFKALSLGAS
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCC
DYIPKPESTREVAAADIFRHDLMQKIRHLAAKRRRPATVASPPPDHDDYGSNASTIMNAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DSNISERDAGGKPRRTFPHPALVQREQQPRSAQAARAMSRPQPTLRSFSAHLPRALLIGS
HCCCHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEC
STGGPQALMTLVAGIGPVIDRCPVLITQHMPPTFTTILAEHLARAAGRPAHEGVDQEIVK
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QGHIYLAPGGRHMRVARKGADAVIVLDNGPAVNFCKPAVDPLFMSAIDVWQGGALAVILT
CCCEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
GMGSDGMRGGTQIVAAGGSIIAQDEASSVVWGMPGAAVQAGICAAVLPLQQIAPKLVRLF
ECCCCCCCCCCEEEEECCCEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGDGL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA