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The map label for this gene is pstS [H]

Identifier: 75674704

GI number: 75674704

Start: 563549

End: 564574

Strand: Direct

Name: pstS [H]

Synonym: Nwi_0506

Alternate gene names: 75674704

Gene position: 563549-564574 (Clockwise)

Preceding gene: 75674703

Following gene: 75674705

Centisome position: 16.56

GC content: 60.53

Gene sequence:

>1026_bases
ATGAATTTCTTCAAGGCCATCGCCGCCGCCGGCCTCGTCGCCGCCTCGGCGTCCGTCTTTTCGGTTCCGGTGAAGGCGGC
CGATGTCACCGGTGCGGGAGCGACCTTTCCGTTTCCCGTCTACTCGAAATGGGCTGATGCCTATAAGAAGGAGACCGGTC
ACGGCGTAAATTATCAGTCGATCGGGTCGGGCGCCGGCATCAAGCAGATCCTTGCCAAGACGGTGACTTTCGGCGCGACC
GACGCGCCGCTGAAGGCCGACAAGCTCGAGAAAGATGGTCTCGCGCAGTGGCCGATGGTGATGGGCGCGATCGTTCCCGT
GGTGAATATCGAGGGCGTGAAGCCCGGCGAACTGGTTCTTTCGGGAGAACTGCTCGGCGATATCTACCTCGGCAAGGTCA
CGAAGTGGGACGATCCGGCGATCGTGAAGCTCAATCCGAGCGTCAAGCTGCCTTCGGCTTCGATCGCGGTGGTGCATCGT
TCGGACGGTGCGGGAACCTCGTTCAATTTCACCAACTATCTGTCCAAGGTGAATGGAGAATGGAAGTCCAAGGTCGGTGA
GGGCACCGCGGTCGAGTGGCCGGCCGGCGTCGGCGCCAAAGGCAATGAAGGCGTTTCCGGCAATGTCGGGCAGACCAGGA
ACGCGATCGGCTATGTCGAGTACGCCTATGCCAAACAGAACAAGCTGACCTACACCGCGCTCATCAACAAGGCCGGCAAG
GTGGTGCAGCCGGGCGTGGCCTCTTTTCAGGCAGCGGCAGCCAATGCCGACTGGGCGAGCGCGCCAGGCTATCACGTGAT
CCTGACCGATCAGCCCGGCGAGGCCTCCTGGCCAATCACCGCGGCGACGTTCATCCTGATGTACAGGGAGCCTTCCGATA
AAGCGGCGTCCGCCGAAGCGATCAAGTTCTTCAAGTGGGCTTTCGAGAAGGGCGGCGTGATGGCGGAGGAACTCGACTAC
ATTCCGATGCCGGAATCTGTCGTGAAGCAGATTGAGAAAACCTGGACCTCCGAGATCAAAAGCTGA

Upstream 100 bases:

>100_bases
CATCCCGCTGTCGTCAAGCGTTCGTAAAAGCGGCAAGGGTCGCATCTAAGAAGGTGTCATGAGCGTCCTGCGCTCTACTC
AATGCGAAGGGAGATCGCGC

Downstream 100 bases:

>100_bases
TCCGGTGCCGAGGAGAAGCGTCGGGGCGCTTCTCCTCGGGAGGGTTGCGAGAAGCTGTTCTCCGTATCCGGATGGTCTGC
AAGAAACAGGGGTGGGGCGT

Product: periplasmic phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNFFKAIAAAGLVAASASVFSVPVKAADVTGAGATFPFPVYSKWADAYKKETGHGVNYQSIGSGAGIKQILAKTVTFGAT
DAPLKADKLEKDGLAQWPMVMGAIVPVVNIEGVKPGELVLSGELLGDIYLGKVTKWDDPAIVKLNPSVKLPSASIAVVHR
SDGAGTSFNFTNYLSKVNGEWKSKVGEGTAVEWPAGVGAKGNEGVSGNVGQTRNAIGYVEYAYAKQNKLTYTALINKAGK
VVQPGVASFQAAAANADWASAPGYHVILTDQPGEASWPITAATFILMYREPSDKAASAEAIKFFKWAFEKGGVMAEELDY
IPMPESVVKQIEKTWTSEIKS

Sequences:

>Translated_341_residues
MNFFKAIAAAGLVAASASVFSVPVKAADVTGAGATFPFPVYSKWADAYKKETGHGVNYQSIGSGAGIKQILAKTVTFGAT
DAPLKADKLEKDGLAQWPMVMGAIVPVVNIEGVKPGELVLSGELLGDIYLGKVTKWDDPAIVKLNPSVKLPSASIAVVHR
SDGAGTSFNFTNYLSKVNGEWKSKVGEGTAVEWPAGVGAKGNEGVSGNVGQTRNAIGYVEYAYAKQNKLTYTALINKAGK
VVQPGVASFQAAAANADWASAPGYHVILTDQPGEASWPITAATFILMYREPSDKAASAEAIKFFKWAFEKGGVMAEELDY
IPMPESVVKQIEKTWTSEIKS
>Mature_341_residues
MNFFKAIAAAGLVAASASVFSVPVKAADVTGAGATFPFPVYSKWADAYKKETGHGVNYQSIGSGAGIKQILAKTVTFGAT
DAPLKADKLEKDGLAQWPMVMGAIVPVVNIEGVKPGELVLSGELLGDIYLGKVTKWDDPAIVKLNPSVKLPSASIAVVHR
SDGAGTSFNFTNYLSKVNGEWKSKVGEGTAVEWPAGVGAKGNEGVSGNVGQTRNAIGYVEYAYAKQNKLTYTALINKAGK
VVQPGVASFQAAAANADWASAPGYHVILTDQPGEASWPITAATFILMYREPSDKAASAEAIKFFKWAFEKGGVMAEELDY
IPMPESVVKQIEKTWTSEIKS

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=326, Percent_Identity=56.1349693251534, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36002; Mature: 36002

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFFKAIAAAGLVAASASVFSVPVKAADVTGAGATFPFPVYSKWADAYKKETGHGVNYQS
CCHHHHHHHHHHHHHCCHHEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHC
IGSGAGIKQILAKTVTFGATDAPLKADKLEKDGLAQWPMVMGAIVPVVNIEGVKPGELVL
CCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEE
SGELLGDIYLGKVTKWDDPAIVKLNPSVKLPSASIAVVHRSDGAGTSFNFTNYLSKVNGE
ECCHHCCEEECCEECCCCCEEEEECCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHCCH
WKSKVGEGTAVEWPAGVGAKGNEGVSGNVGQTRNAIGYVEYAYAKQNKLTYTALINKAGK
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEHHCCCC
VVQPGVASFQAAAANADWASAPGYHVILTDQPGEASWPITAATFILMYREPSDKAASAEA
CCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHEEEEEEEEECCCCCHHHHHH
IKFFKWAFEKGGVMAEELDYIPMPESVVKQIEKTWTSEIKS
HHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFFKAIAAAGLVAASASVFSVPVKAADVTGAGATFPFPVYSKWADAYKKETGHGVNYQS
CCHHHHHHHHHHHHHCCHHEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHC
IGSGAGIKQILAKTVTFGATDAPLKADKLEKDGLAQWPMVMGAIVPVVNIEGVKPGELVL
CCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEE
SGELLGDIYLGKVTKWDDPAIVKLNPSVKLPSASIAVVHRSDGAGTSFNFTNYLSKVNGE
ECCHHCCEEECCEECCCCCEEEEECCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHCCH
WKSKVGEGTAVEWPAGVGAKGNEGVSGNVGQTRNAIGYVEYAYAKQNKLTYTALINKAGK
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEHHCCCC
VVQPGVASFQAAAANADWASAPGYHVILTDQPGEASWPITAATFILMYREPSDKAASAEA
CCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHEEEEEEEEECCCCCHHHHHH
IKFFKWAFEKGGVMAEELDYIPMPESVVKQIEKTWTSEIKS
HHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]