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The map label for this gene is ispG [H]

Identifier: 75674692

GI number: 75674692

Start: 550968

End: 552260

Strand: Direct

Name: ispG [H]

Synonym: Nwi_0494

Alternate gene names: 75674692

Gene position: 550968-552260 (Clockwise)

Preceding gene: 75674691

Following gene: 75674694

Centisome position: 16.19

GC content: 65.51

Gene sequence:

>1293_bases
ATGAACAAGCTCGAAAGTCCGTCGGAGATCGACGTCGCGGGTCCCAGCCCGCGGCACAAGACCACGCAGGTGATGGTCGG
CAATGTCGCGGTCGGCGGCGGCGCGCCGATCGTGGTGCAATCGATGACCAACACCGACACCGCCGACATCGAGGGCACGA
TCGCCCAGGTCGCCGCCTTGTCGCGCGCCGGGTCGGAAATGGTCCGCATGACGGTGGACCGCGACGAGGCGGCAGCCGCC
GTGCCGCATATCCGCGACGGCTTGCGCAAGCGCGGCATCACCACGCCGCTGATCGGCGATTTCCATTATATCGGCCACAA
GCTGCTGGCCGATCATCCGTCCTGCGCCGAGGCGCTCGACAAGTACCGGATCAATCCGGGCAATGTCGGTTTCAAGGACA
AGCGCGACAAGCAATTCACCGACATCGTCGAAACCGCGATCAAATACGGCAAGGCGGTGCGGATCGGCGCCAACTGGGGT
TCGCTCGATCAGGAACTGCTGACGCACCTGATGGAAGAGAACGCCAATTCGGCCGCGCCGCTCGACGCCCGCGCTGTGAC
TCGCGAGGCGATGGTGCAATCGGCGCTACTGTCGGCGAAGCGCGCCGAGGAGATCGGTCTGCCGAAGACCAGGATGGTTC
TCTCGGCGAAGGTCTCGGCGGTGCAGGATCTGATCGCCGTCTACCAGACGCTGGCGTCGCGTTCGGACTACGCGATCCAT
CTCGGCCTCACCGAGGCCGGCATGGGCTCGAAGGGCATCGTCGCATCATCTGCCGCGCTCGGCATCCTGCTCCAGCAGGG
CATCGGCGACACCATCCGGATCTCGCTGACGCCGGAGCCCGGCGGAGACCGCACGCTGGAAGTTCAGGTCGCGCAGGAAC
TGTTGCAGACCATGGGCTTCCGCACCTTCGTGCCGCTGGTTGCGGCCTGTCCGGGCTGCGGGCGCACCACCTCCACCACG
TTCCAGGAATTGGCGCGGTCGATCCAGGATTTCATCCGCGACGAGATGCCGAACTGGAAGACGCAATATCCCGGCGTCGA
GCAGCTCAACGTCGCGGTCATGGGCTGCATCGTCAATGGTCCCGGCGAATCCAAGCATGCCGATATCGGCATCTCGCTGC
CCGGCACGGGCGAGGCGCCGGCCGCGCCGGTGTTTGTGGACGGCAAGAAGTTCAAGACGCTGCGAGGACCGGCGATCGCG
CAGGACTTCAAGGCGCTGGTGATCGATTACATCGAGCAGCGTTATGGCGACGCCGCCAAGGCGCGGGCCGAAGCGGTGAG
CGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GCGGCGCTGTGGTAAATGCTGGCCACCGACCTGCAGGGCGCTTCTTAACTCTGGCATTGATCCGTTGCTGAACATTACGA
AAACCTGATATTTGAGGCCC

Downstream 100 bases:

>100_bases
CCGGCTTATAGCGTTTTCGAGCGAAGCATGTCCTCGGGGCCTGAGCCCTAAGGATGGAAATCGGTTCGCGTGAAGAAAAC
GCGTCAAAACAACAATTTAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNKLESPSEIDVAGPSPRHKTTQVMVGNVAVGGGAPIVVQSMTNTDTADIEGTIAQVAALSRAGSEMVRMTVDRDEAAAA
VPHIRDGLRKRGITTPLIGDFHYIGHKLLADHPSCAEALDKYRINPGNVGFKDKRDKQFTDIVETAIKYGKAVRIGANWG
SLDQELLTHLMEENANSAAPLDARAVTREAMVQSALLSAKRAEEIGLPKTRMVLSAKVSAVQDLIAVYQTLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTT
FQELARSIQDFIRDEMPNWKTQYPGVEQLNVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFVDGKKFKTLRGPAIA
QDFKALVIDYIEQRYGDAAKARAEAVSAAE

Sequences:

>Translated_430_residues
MNKLESPSEIDVAGPSPRHKTTQVMVGNVAVGGGAPIVVQSMTNTDTADIEGTIAQVAALSRAGSEMVRMTVDRDEAAAA
VPHIRDGLRKRGITTPLIGDFHYIGHKLLADHPSCAEALDKYRINPGNVGFKDKRDKQFTDIVETAIKYGKAVRIGANWG
SLDQELLTHLMEENANSAAPLDARAVTREAMVQSALLSAKRAEEIGLPKTRMVLSAKVSAVQDLIAVYQTLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTT
FQELARSIQDFIRDEMPNWKTQYPGVEQLNVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFVDGKKFKTLRGPAIA
QDFKALVIDYIEQRYGDAAKARAEAVSAAE
>Mature_430_residues
MNKLESPSEIDVAGPSPRHKTTQVMVGNVAVGGGAPIVVQSMTNTDTADIEGTIAQVAALSRAGSEMVRMTVDRDEAAAA
VPHIRDGLRKRGITTPLIGDFHYIGHKLLADHPSCAEALDKYRINPGNVGFKDKRDKQFTDIVETAIKYGKAVRIGANWG
SLDQELLTHLMEENANSAAPLDARAVTREAMVQSALLSAKRAEEIGLPKTRMVLSAKVSAVQDLIAVYQTLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTT
FQELARSIQDFIRDEMPNWKTQYPGVEQLNVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFVDGKKFKTLRGPAIA
QDFKALVIDYIEQRYGDAAKARAEAVSAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=378, Percent_Identity=33.0687830687831, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45756; Mature: 45756

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLESPSEIDVAGPSPRHKTTQVMVGNVAVGGGAPIVVQSMTNTDTADIEGTIAQVAAL
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHH
SRAGSEMVRMTVDRDEAAAAVPHIRDGLRKRGITTPLIGDFHYIGHKLLADHPSCAEALD
HHCCCHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
KYRINPGNVGFKDKRDKQFTDIVETAIKYGKAVRIGANWGSLDQELLTHLMEENANSAAP
HCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCC
LDARAVTREAMVQSALLSAKRAEEIGLPKTRMVLSAKVSAVQDLIAVYQTLASRSDYAIH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGF
EECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCH
RTFVPLVAACPGCGRTTSTTFQELARSIQDFIRDEMPNWKTQYPGVEQLNVAVMGCIVNG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEEEECC
PGESKHADIGISLPGTGEAPAAPVFVDGKKFKTLRGPAIAQDFKALVIDYIEQRYGDAAK
CCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHH
ARAEAVSAAE
HHHHHHHCCC
>Mature Secondary Structure
MNKLESPSEIDVAGPSPRHKTTQVMVGNVAVGGGAPIVVQSMTNTDTADIEGTIAQVAAL
CCCCCCCCCEECCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHH
SRAGSEMVRMTVDRDEAAAAVPHIRDGLRKRGITTPLIGDFHYIGHKLLADHPSCAEALD
HHCCCHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
KYRINPGNVGFKDKRDKQFTDIVETAIKYGKAVRIGANWGSLDQELLTHLMEENANSAAP
HCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCC
LDARAVTREAMVQSALLSAKRAEEIGLPKTRMVLSAKVSAVQDLIAVYQTLASRSDYAIH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTLEVQVAQELLQTMGF
EECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCH
RTFVPLVAACPGCGRTTSTTFQELARSIQDFIRDEMPNWKTQYPGVEQLNVAVMGCIVNG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEEEECC
PGESKHADIGISLPGTGEAPAAPVFVDGKKFKTLRGPAIAQDFKALVIDYIEQRYGDAAK
CCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHH
ARAEAVSAAE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA