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The map label for this gene is folD [H]

Identifier: 75674678

GI number: 75674678

Start: 536741

End: 537625

Strand: Direct

Name: folD [H]

Synonym: Nwi_0480

Alternate gene names: 75674678

Gene position: 536741-537625 (Clockwise)

Preceding gene: 75674677

Following gene: 75674679

Centisome position: 15.78

GC content: 67.01

Gene sequence:

>885_bases
ATGGCGGCCAGTATCATCGATGGAAAAGTCATCGCCGCCGATTTGCGCGCGCGGGTGGCCGGTGAGGTGACGCGGATCAA
GCGTGATCACGGGTTGACGCCGGGCCTCGCCGTGGTGCTGGTCGGCAACGATCCGGCGAGCGAAGTCTACGTCCGCAACA
AGCATAAGCAGACCCAGGCCGCCGGCATGGCGTCGTTCGAGCACATGCTGCCCGCGGATGTCGCCCAGGCCGATGTGCTG
GCCCTGATCGCTGAACTGAATGCGGATCCCGCCGTTCACGGCATTCTGGTTCAACTGCCGCTGCCGAAGGGACTCGACAC
CGAGGCGATCATCGCCGCCATCGATCCGGCCAAGGATGTCGACGGGCTGCATCCCCACAACGCCGGCCGCCTGGCAGGCG
GCCTGTCGGCCCTGTCGCCATGCACGCCGCTTGGCTGCATTATTCTGACCAAAAGCGTGCATGCCTCGCTCGAAGGTCTG
GACGCCATCGTGATCGGACGCTCCAATCTCGTGGGCCGGCCGCTGGTGCAGTTGCTGCTCAATGAAAACGCCACGGTGAC
GATCGCGCATTCGCGCTCGCGCAACCTGCCCGAACTCTGTCGCCGCGCCGATCTGGTCTATGCGGCGGTCGGCAGGGCTG
AAATGGTGCGCGGCGACTGGCTCAAGCCGGGCGCGACGGTGATCGATGTCGGCATCACGCGCGTGCCGGCGGCCGAGGGT
AAAACCCGGTTGATCGGCGACGTGGCGTTCGATGAGGCGATGCAGGTCGCGGGGGCTGTGACGCCGGTACCCGGCGGCGT
CGGGCAGATGACCGTGGCTTGCCTGCTGGTCAACACGCTGCGCGCGGCCTGCGCGATCGAGGGGCTGTCCGCGCCGGGGG
TGTAG

Upstream 100 bases:

>100_bases
GCTGAAGCAGGTCGCGGTCGAGGGCGATCCGGTCCAGCTCGGCAACGCCCTGCGCGCATTGACGGCGGTTCAACCGAAAT
AGCCTGAACTGGGGATCAGC

Downstream 100 bases:

>100_bases
GCAGCTTTTGACGAAAGGCTCCGTATGCCGACCTCGGCGTACAACGATACTGGTGCGGCTATAGCGTTTTCGAGCGAAGG
GCACCGGTTCGCGTGAAGAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MAASIIDGKVIAADLRARVAGEVTRIKRDHGLTPGLAVVLVGNDPASEVYVRNKHKQTQAAGMASFEHMLPADVAQADVL
ALIAELNADPAVHGILVQLPLPKGLDTEAIIAAIDPAKDVDGLHPHNAGRLAGGLSALSPCTPLGCIILTKSVHASLEGL
DAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRNLPELCRRADLVYAAVGRAEMVRGDWLKPGATVIDVGITRVPAAEG
KTRLIGDVAFDEAMQVAGAVTPVPGGVGQMTVACLLVNTLRAACAIEGLSAPGV

Sequences:

>Translated_294_residues
MAASIIDGKVIAADLRARVAGEVTRIKRDHGLTPGLAVVLVGNDPASEVYVRNKHKQTQAAGMASFEHMLPADVAQADVL
ALIAELNADPAVHGILVQLPLPKGLDTEAIIAAIDPAKDVDGLHPHNAGRLAGGLSALSPCTPLGCIILTKSVHASLEGL
DAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRNLPELCRRADLVYAAVGRAEMVRGDWLKPGATVIDVGITRVPAAEG
KTRLIGDVAFDEAMQVAGAVTPVPGGVGQMTVACLLVNTLRAACAIEGLSAPGV
>Mature_293_residues
AASIIDGKVIAADLRARVAGEVTRIKRDHGLTPGLAVVLVGNDPASEVYVRNKHKQTQAAGMASFEHMLPADVAQADVLA
LIAELNADPAVHGILVQLPLPKGLDTEAIIAAIDPAKDVDGLHPHNAGRLAGGLSALSPCTPLGCIILTKSVHASLEGLD
AIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRNLPELCRRADLVYAAVGRAEMVRGDWLKPGATVIDVGITRVPAAEGK
TRLIGDVAFDEAMQVAGAVTPVPGGVGQMTVACLLVNTLRAACAIEGLSAPGV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=44.2953020134228, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=41.3559322033898, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=40, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI36796743, Length=206, Percent_Identity=25.7281553398058, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=48.0565371024735, Blast_Score=246, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=40.893470790378, Blast_Score=200, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=284, Percent_Identity=44.7183098591549, Blast_Score=249, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.9530201342282, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=28.2051282051282, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=46.3414634146341, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=46.3414634146341, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=46.6898954703833, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=46.6898954703833, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=45.2054794520548, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=45.2054794520548, Blast_Score=223, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30341; Mature: 30209

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASIIDGKVIAADLRARVAGEVTRIKRDHGLTPGLAVVLVGNDPASEVYVRNKHKQTQA
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHEEECCCHHHHH
AGMASFEHMLPADVAQADVLALIAELNADPAVHGILVQLPLPKGLDTEAIIAAIDPAKDV
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCC
DGLHPHNAGRLAGGLSALSPCTPLGCIILTKSVHASLEGLDAIVIGRSNLVGRPLVQLLL
CCCCCCCCCHHHCCHHHCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRSRNLPELCRRADLVYAAVGRAEMVRGDWLKPGATVIDVGITRVPAAEG
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCEECCCCCC
KTRLIGDVAFDEAMQVAGAVTPVPGGVGQMTVACLLVNTLRAACAIEGLSAPGV
CCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AASIIDGKVIAADLRARVAGEVTRIKRDHGLTPGLAVVLVGNDPASEVYVRNKHKQTQA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHEEECCCHHHHH
AGMASFEHMLPADVAQADVLALIAELNADPAVHGILVQLPLPKGLDTEAIIAAIDPAKDV
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCC
DGLHPHNAGRLAGGLSALSPCTPLGCIILTKSVHASLEGLDAIVIGRSNLVGRPLVQLLL
CCCCCCCCCHHHCCHHHCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRSRNLPELCRRADLVYAAVGRAEMVRGDWLKPGATVIDVGITRVPAAEG
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCEECCCCCC
KTRLIGDVAFDEAMQVAGAVTPVPGGVGQMTVACLLVNTLRAACAIEGLSAPGV
CCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA