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The map label for this gene is priA [H]

Identifier: 75674625

GI number: 75674625

Start: 480969

End: 483185

Strand: Direct

Name: priA [H]

Synonym: Nwi_0427

Alternate gene names: 75674625

Gene position: 480969-483185 (Clockwise)

Preceding gene: 75674623

Following gene: 229125527

Centisome position: 14.14

GC content: 67.7

Gene sequence:

>2217_bases
ATGGACCGTTCTTCGCGCCATGGCACAATCCCGGCATCGTCTTCGGCGCCGCGCGTCGTCGATGTGCTGGTTCCCGTCGC
GATCGATCACGCCTATTCCTATCGCGTGCCGAGAGGGATGGAGGTGCGGCCGGGCGAGGTGGTGACGGTGCCGCTCGGCC
CTCGCGAGGTGGTCGCCGTGGTCTGGGCCGGGAACCCCGCGCCGGATCCGCGCCTGCACAACCGCCTCAAGGACATCTCC
GGCAAACTCGATGTCCCACCGCTCAGGGACGAACTGCGCACGCTGATCGACTGGGTCGCCAATTACACGCTGTCGCCGCG
CGGCATGGTGATGCGCATGAGCCTGCGGATGGGCGAGCACCTCGGGCCGGAACGGGTGCGGCCGGGCGTGCGGCTGAGCG
GCGAGCCGCCCCGGCGCATGACCCCGGCGCGGCGGCGGTTGCTGGCGGTCCTGTCGGACGGACTGCTGCGGGCGAAATCC
GATGCGGCGAGGGAAGCGGGCGTCAGCGCCGGCGTTGTCGACGGCCTCGTCGATGAGGGCACGCTGACGACCGAGCCGAT
GCCACGGTCGCCGCCGCCTCCGCCGCCGGACCCGGATTTCGCCGTGCCCGATTTCTCCAGGTCGCAGCGCGCGGCCGTGG
ACGCCATGCGCGCGCTGGCGTCGAACGGTACATTCCACGTCGCGCTGCTCGACGGCGTGACCGGCTCCGGCAAAACCGAA
GTCTATTTTGAAGCCGTCGCCGAAACCGTCCGCCGCGGCAGGCAGGTTTTGATCCTGATGCCCGAGATCGCGTTGACCGG
GCAGTTCCGCGACCGTTTCGCGGCCCGCTTCGGCGAGCGTCCGCTGGAGTGGCATTCCGAATTGACGCCACGCAATCGCG
CGCGCAACTGGGCCGCGATCGCGGCGGGTGAGGCGCAGGTCGTGGTCGGCGCGCGCTCAGCGCTGTTTCTTCCCTATGCC
GACCTCGGACTGATCATCATCGATGAGGAGCACGACCAGGCCTACAAGCAGGACGAAGGCGTGCGCTATCACGCCCGCGA
CATGGCCGTGGTTCGCGCGCATGTCGCGAAGATTCCCATCATACTGGCCTCGGCGACTCCGTCCGTCGAAAGCGAGGTCA
ATGCGCGCAAGGGACGCTATCAACGCATCGCGCTGACATCCCGATTCGGCGGCCAGCATTTGCCGCGGATCGAACTGATC
GATCTGCGGCGCGAGGGACCCTCGCGCGGCCGCTTCATTTCTCCGCGTCTGGCGGGAGAGATCGTGACCGCCATCGAGCG
GCGCGAGCAGGCGCTGCTGTTTCTCAATCGCCGCGGTTACGCGCCGCTGACCTTGTGCCGCGGCTGCGGCCACCGCTTCG
CCTGCACCATCTGTGATGCCTGGCTGGTGGATCACCGCTTCCGGGACCGGCTCGTATGCCATCACTGCGGCTTCTCGATG
CCGCGCCCGCATCAATGTCCGCATTGCGCTGCCGAACAATCGCTGGTCGCGGTCGGCCCCGGCGTCGAACGGCTGCAGGA
GGAGGCCGCCGCGCTATTCCCCGACGCACGGACCATGGTGCTGTCGAGCGATCTGATCACCTCGATCGAGACCATGCGCT
CGGAATTGAACGACATCGCCGAGGGCCGCGTCGACATCATCATCGGCACGCAACTGGTGGCGAAGGGACATCATTTTCCG
CGCCTGAACCTCGTCGGAGTGGTGGACGCCGACCTCGGCCTCGGCAACGGCGATCCCCGCGCCGCGGAGCGAACCTTCCA
ACTGCTCAATCAGGTGATCGGCCGCACCGGCCGCGAACAGGGTCGCGGCGTCGGCTATCTGCAAACCCATCAGCCCGAAC
ATCCCGTGATGAAGGCCCTGGTCGCGGGCGATCGCGAGGCGTTCTATGCCAGCGAAATCGCCGCGCGTGAGCGGACCGGC
TACCCGCCGTTCGGCCGGCTGGCCAGCCTGATCATATCCGCGGGTGATCGCCCGAGCGCGGAGAGCTTCGCCCGCCAACT
CGCCGCGGTCGCGCCGGTCGACGAACGCATCCAGGTGCTCGGACCGGCGGAAGCTCCGCTGGCCCTGATCAAGGGACGCT
ATCGATTCCGCCTGCTGGTGAAGTCGGCGCGCGGCGTGGATCTCTCCGGCTATTTGCGCGACTGGCTCGCGTCCGCGCCG
AAGACGAGAGGCAATCTCAAGCTCGAAGTGGATGTCGATCCGCAGAGCTTTCTTTGA

Upstream 100 bases:

>100_bases
CGCCGCTTTCGTTTATCCTTCGCTAATCTTCCGGTTGGCTGGCCTATGGTGGTGCCGTCCTAAATTGTTAACCCGATCTC
TGATCGAGCTGATTCTCCCG

Downstream 100 bases:

>100_bases
GAAGCCCCGCTTCTGATGGAATCAGAAGCGGGGCTTTTTATGATCTTGATCTGACGCGTTTTGAGAAGCGAAACGCGGTG
TGGGAACTGGTCTACATGAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MDRSSRHGTIPASSSAPRVVDVLVPVAIDHAYSYRVPRGMEVRPGEVVTVPLGPREVVAVVWAGNPAPDPRLHNRLKDIS
GKLDVPPLRDELRTLIDWVANYTLSPRGMVMRMSLRMGEHLGPERVRPGVRLSGEPPRRMTPARRRLLAVLSDGLLRAKS
DAAREAGVSAGVVDGLVDEGTLTTEPMPRSPPPPPPDPDFAVPDFSRSQRAAVDAMRALASNGTFHVALLDGVTGSGKTE
VYFEAVAETVRRGRQVLILMPEIALTGQFRDRFAARFGERPLEWHSELTPRNRARNWAAIAAGEAQVVVGARSALFLPYA
DLGLIIIDEEHDQAYKQDEGVRYHARDMAVVRAHVAKIPIILASATPSVESEVNARKGRYQRIALTSRFGGQHLPRIELI
DLRREGPSRGRFISPRLAGEIVTAIERREQALLFLNRRGYAPLTLCRGCGHRFACTICDAWLVDHRFRDRLVCHHCGFSM
PRPHQCPHCAAEQSLVAVGPGVERLQEEAAALFPDARTMVLSSDLITSIETMRSELNDIAEGRVDIIIGTQLVAKGHHFP
RLNLVGVVDADLGLGNGDPRAAERTFQLLNQVIGRTGREQGRGVGYLQTHQPEHPVMKALVAGDREAFYASEIAARERTG
YPPFGRLASLIISAGDRPSAESFARQLAAVAPVDERIQVLGPAEAPLALIKGRYRFRLLVKSARGVDLSGYLRDWLASAP
KTRGNLKLEVDVDPQSFL

Sequences:

>Translated_738_residues
MDRSSRHGTIPASSSAPRVVDVLVPVAIDHAYSYRVPRGMEVRPGEVVTVPLGPREVVAVVWAGNPAPDPRLHNRLKDIS
GKLDVPPLRDELRTLIDWVANYTLSPRGMVMRMSLRMGEHLGPERVRPGVRLSGEPPRRMTPARRRLLAVLSDGLLRAKS
DAAREAGVSAGVVDGLVDEGTLTTEPMPRSPPPPPPDPDFAVPDFSRSQRAAVDAMRALASNGTFHVALLDGVTGSGKTE
VYFEAVAETVRRGRQVLILMPEIALTGQFRDRFAARFGERPLEWHSELTPRNRARNWAAIAAGEAQVVVGARSALFLPYA
DLGLIIIDEEHDQAYKQDEGVRYHARDMAVVRAHVAKIPIILASATPSVESEVNARKGRYQRIALTSRFGGQHLPRIELI
DLRREGPSRGRFISPRLAGEIVTAIERREQALLFLNRRGYAPLTLCRGCGHRFACTICDAWLVDHRFRDRLVCHHCGFSM
PRPHQCPHCAAEQSLVAVGPGVERLQEEAAALFPDARTMVLSSDLITSIETMRSELNDIAEGRVDIIIGTQLVAKGHHFP
RLNLVGVVDADLGLGNGDPRAAERTFQLLNQVIGRTGREQGRGVGYLQTHQPEHPVMKALVAGDREAFYASEIAARERTG
YPPFGRLASLIISAGDRPSAESFARQLAAVAPVDERIQVLGPAEAPLALIKGRYRFRLLVKSARGVDLSGYLRDWLASAP
KTRGNLKLEVDVDPQSFL
>Mature_738_residues
MDRSSRHGTIPASSSAPRVVDVLVPVAIDHAYSYRVPRGMEVRPGEVVTVPLGPREVVAVVWAGNPAPDPRLHNRLKDIS
GKLDVPPLRDELRTLIDWVANYTLSPRGMVMRMSLRMGEHLGPERVRPGVRLSGEPPRRMTPARRRLLAVLSDGLLRAKS
DAAREAGVSAGVVDGLVDEGTLTTEPMPRSPPPPPPDPDFAVPDFSRSQRAAVDAMRALASNGTFHVALLDGVTGSGKTE
VYFEAVAETVRRGRQVLILMPEIALTGQFRDRFAARFGERPLEWHSELTPRNRARNWAAIAAGEAQVVVGARSALFLPYA
DLGLIIIDEEHDQAYKQDEGVRYHARDMAVVRAHVAKIPIILASATPSVESEVNARKGRYQRIALTSRFGGQHLPRIELI
DLRREGPSRGRFISPRLAGEIVTAIERREQALLFLNRRGYAPLTLCRGCGHRFACTICDAWLVDHRFRDRLVCHHCGFSM
PRPHQCPHCAAEQSLVAVGPGVERLQEEAAALFPDARTMVLSSDLITSIETMRSELNDIAEGRVDIIIGTQLVAKGHHFP
RLNLVGVVDADLGLGNGDPRAAERTFQLLNQVIGRTGREQGRGVGYLQTHQPEHPVMKALVAGDREAFYASEIAARERTG
YPPFGRLASLIISAGDRPSAESFARQLAAVAPVDERIQVLGPAEAPLALIKGRYRFRLLVKSARGVDLSGYLRDWLASAP
KTRGNLKLEVDVDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=751, Percent_Identity=35.6857523302264, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 81060; Mature: 81060

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSSRHGTIPASSSAPRVVDVLVPVAIDHAYSYRVPRGMEVRPGEVVTVPLGPREVVAV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEEEECCCCCEEEE
VWAGNPAPDPRLHNRLKDISGKLDVPPLRDELRTLIDWVANYTLSPRGMVMRMSLRMGEH
EECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCC
LGPERVRPGVRLSGEPPRRMTPARRRLLAVLSDGLLRAKSDAAREAGVSAGVVDGLVDEG
CCHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCHHHCCCHHHHHCCCCHHHHHHHCCCC
TLTTEPMPRSPPPPPPDPDFAVPDFSRSQRAAVDAMRALASNGTFHVALLDGVTGSGKTE
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCH
VYFEAVAETVRRGRQVLILMPEIALTGQFRDRFAARFGERPLEWHSELTPRNRARNWAAI
HHHHHHHHHHHCCCEEEEEECCHHCCCHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEEE
AAGEAQVVVGARSALFLPYADLGLIIIDEEHDQAYKQDEGVRYHARDMAVVRAHVAKIPI
ECCCEEEEEECCCEEEEEECCCCEEEEECCHHHHHHCCCCCEEEHHHHHHHHHHHHHCCE
ILASATPSVESEVNARKGRYQRIALTSRFGGQHLPRIELIDLRREGPSRGRFISPRLAGE
EEECCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCEECHHHHHH
IVTAIERREQALLFLNRRGYAPLTLCRGCGHRFACTICDAWLVDHRFRDRLVCHHCGFSM
HHHHHHHHHHEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCC
PRPHQCPHCAAEQSLVAVGPGVERLQEEAAALFPDARTMVLSSDLITSIETMRSELNDIA
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHH
EGRVDIIIGTQLVAKGHHFPRLNLVGVVDADLGLGNGDPRAAERTFQLLNQVIGRTGREQ
CCCEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
GRGVGYLQTHQPEHPVMKALVAGDREAFYASEIAARERTGYPPFGRLASLIISAGDRPSA
CCCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCH
ESFARQLAAVAPVDERIQVLGPAEAPLALIKGRYRFRLLVKSARGVDLSGYLRDWLASAP
HHHHHHHHHHCCHHHHHEEECCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCC
KTRGNLKLEVDVDPQSFL
CCCCCEEEEEECCHHHCC
>Mature Secondary Structure
MDRSSRHGTIPASSSAPRVVDVLVPVAIDHAYSYRVPRGMEVRPGEVVTVPLGPREVVAV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEEEECCCCCEEEE
VWAGNPAPDPRLHNRLKDISGKLDVPPLRDELRTLIDWVANYTLSPRGMVMRMSLRMGEH
EECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCC
LGPERVRPGVRLSGEPPRRMTPARRRLLAVLSDGLLRAKSDAAREAGVSAGVVDGLVDEG
CCHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCHHHCCCHHHHHCCCCHHHHHHHCCCC
TLTTEPMPRSPPPPPPDPDFAVPDFSRSQRAAVDAMRALASNGTFHVALLDGVTGSGKTE
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCH
VYFEAVAETVRRGRQVLILMPEIALTGQFRDRFAARFGERPLEWHSELTPRNRARNWAAI
HHHHHHHHHHHCCCEEEEEECCHHCCCHHHHHHHHHHCCCCHHHHHCCCCCHHCCCEEEE
AAGEAQVVVGARSALFLPYADLGLIIIDEEHDQAYKQDEGVRYHARDMAVVRAHVAKIPI
ECCCEEEEEECCCEEEEEECCCCEEEEECCHHHHHHCCCCCEEEHHHHHHHHHHHHHCCE
ILASATPSVESEVNARKGRYQRIALTSRFGGQHLPRIELIDLRREGPSRGRFISPRLAGE
EEECCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCEECHHHHHH
IVTAIERREQALLFLNRRGYAPLTLCRGCGHRFACTICDAWLVDHRFRDRLVCHHCGFSM
HHHHHHHHHHEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCC
PRPHQCPHCAAEQSLVAVGPGVERLQEEAAALFPDARTMVLSSDLITSIETMRSELNDIA
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHH
EGRVDIIIGTQLVAKGHHFPRLNLVGVVDADLGLGNGDPRAAERTFQLLNQVIGRTGREQ
CCCEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
GRGVGYLQTHQPEHPVMKALVAGDREAFYASEIAARERTGYPPFGRLASLIISAGDRPSA
CCCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCH
ESFARQLAAVAPVDERIQVLGPAEAPLALIKGRYRFRLLVKSARGVDLSGYLRDWLASAP
HHHHHHHHHHCCHHHHHEEECCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCC
KTRGNLKLEVDVDPQSFL
CCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]