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The map label for this gene is lpd3 [H]

Identifier: 75674623

GI number: 75674623

Start: 478320

End: 479723

Strand: Direct

Name: lpd3 [H]

Synonym: Nwi_0425

Alternate gene names: 75674623

Gene position: 478320-479723 (Clockwise)

Preceding gene: 75674622

Following gene: 75674625

Centisome position: 14.06

GC content: 63.25

Gene sequence:

>1404_bases
ATGACGACATACGACCTCATCATTATCGGCACCGGTCCCGGCGGCTACGTCTGCGCGGTTCGCGCGGCGCAGCTCGGCAT
GAAAGTCGCCGTCGTCGAGAAGAACCCGACGCTCGGCGGCACCTGCCTCAATGTCGGCTGCATGCCGTCGAAGGCGCTGC
TGCACGCCTCCGAGGCGTTCCAGGAAGCAGGACATTCGTTCGCAAGAATGGGCATCGGCGTCAGCGCGCCGAAGCTAGAT
CTGTCCGCGATGATGGACTTCAAGCAGCAGGGTATCGACGGCAACGTCAAGGGCGTCGAGTACCTGATGAAGAAGAACAA
GATCGACGTGCTGCGGGGCGCCGGCCGCGTCGTCGCAGCCGGCCAGGTCGAGGTCACCGGGAAGGACGGCAAGATACAGA
CGGCCGAAACGAAGAACATCGTGATCGCCACCGGCTCCGATGCCGCGAAACTCAAGGGAGTCGAGATCGACGAGAAGCGT
ATCGTGTCGTCGACCGGTGCGCTGTCGCTCGACAAGGTGCCTGAAAAACTTCTGGTCGTCGGAGCGGGCGTGATCGGGCT
CGAACTCGGCTCGGTGTGGCGCCGCCTCGGCGCGCAGGTCACGGTGGTGGAATTTCTCGACCGTATCCTGCCCGGCATGG
ACGGCGAGATCGCGAAGCAATTTCAGCGCATTCTCGAAAAGCAGGGCTTTGCATTCAAGCTCGGAGCCAAGGTCACCGGC
GTCGATACATCAGGCAAGACGCTGTCGGCTCGGATCGAGCCCGCAGCGGGCGGCGCAGCCGAGACGATCGAAGCCGATGT
AGTGCTGGTCGCGATCGGCCGCGCGCCCTATACCAATGGCCTCGGTCTCAAGGAAGCAGGCGTCGCGCTCGATGACCGCG
GCCGCGTGGAGATCGACAAACATTTCGCGACCAGCGTGAAGGGCATTTACGCCATCGGCGATGTCGTCCGGGGACCGATG
CTTGCCCACAAGGCCGCGGACGAGGGCGTGGCGTGCGCGGAGATCCTCGCAGGCCAGGCCGGCCATGTGAACTATGACGT
GATCCCCGGCGTGATCTACACAACGCCAGAGGTGTCATCGGTCGGCAAGACCGAGGAGGAACTGAAGCAGGCCGGCACGG
CCTATGCCGTCGGCAAGTTTCCGTTCACAGCGAATGGCCGCTCCAAGGTCAATCAGACGACAGACGGTTTCGTGAAGATT
CTGGCGGACGCGAAAACCGACCGCGTGCTCGGCGTTCACATCATCGGTCGCGAGGCCGGCGAACTGATCCATGAAGCCTG
TGTGCTGATGGAATTCGGCGGCTCGGCGGAAGATCTCGCGCGCACCTGCCACGCGCACCCGACGCGATCGGAAGCGATCA
AGGAAGCCGCCCTCGCCGTTGGAAAACGCGCCATTCACATGTGA

Upstream 100 bases:

>100_bases
GCCGATCCCGACGAACTCAACCGGCTGGTCGGAGGATTGCTCTTCGAGCAGCGCGCAAAATTGATTCCCGGGCAGGACGA
GGCAGGCTGAGCAGGCACTC

Downstream 100 bases:

>100_bases
GACTGCGAGGACGGCAAGCTTCCGCTCTGCGATCCTAGAGCCTTTTCGCTTCTGATGGAATCAGAAGCGAGGCTCTATAA
TCTTGATTTGACGCGTTTTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTTYDLIIIGTGPGGYVCAVRAAQLGMKVAVVEKNPTLGGTCLNVGCMPSKALLHASEAFQEAGHSFARMGIGVSAPKLD
LSAMMDFKQQGIDGNVKGVEYLMKKNKIDVLRGAGRVVAAGQVEVTGKDGKIQTAETKNIVIATGSDAAKLKGVEIDEKR
IVSSTGALSLDKVPEKLLVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDGEIAKQFQRILEKQGFAFKLGAKVTG
VDTSGKTLSARIEPAAGGAAETIEADVVLVAIGRAPYTNGLGLKEAGVALDDRGRVEIDKHFATSVKGIYAIGDVVRGPM
LAHKAADEGVACAEILAGQAGHVNYDVIPGVIYTTPEVSSVGKTEEELKQAGTAYAVGKFPFTANGRSKVNQTTDGFVKI
LADAKTDRVLGVHIIGREAGELIHEACVLMEFGGSAEDLARTCHAHPTRSEAIKEAALAVGKRAIHM

Sequences:

>Translated_467_residues
MTTYDLIIIGTGPGGYVCAVRAAQLGMKVAVVEKNPTLGGTCLNVGCMPSKALLHASEAFQEAGHSFARMGIGVSAPKLD
LSAMMDFKQQGIDGNVKGVEYLMKKNKIDVLRGAGRVVAAGQVEVTGKDGKIQTAETKNIVIATGSDAAKLKGVEIDEKR
IVSSTGALSLDKVPEKLLVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDGEIAKQFQRILEKQGFAFKLGAKVTG
VDTSGKTLSARIEPAAGGAAETIEADVVLVAIGRAPYTNGLGLKEAGVALDDRGRVEIDKHFATSVKGIYAIGDVVRGPM
LAHKAADEGVACAEILAGQAGHVNYDVIPGVIYTTPEVSSVGKTEEELKQAGTAYAVGKFPFTANGRSKVNQTTDGFVKI
LADAKTDRVLGVHIIGREAGELIHEACVLMEFGGSAEDLARTCHAHPTRSEAIKEAALAVGKRAIHM
>Mature_466_residues
TTYDLIIIGTGPGGYVCAVRAAQLGMKVAVVEKNPTLGGTCLNVGCMPSKALLHASEAFQEAGHSFARMGIGVSAPKLDL
SAMMDFKQQGIDGNVKGVEYLMKKNKIDVLRGAGRVVAAGQVEVTGKDGKIQTAETKNIVIATGSDAAKLKGVEIDEKRI
VSSTGALSLDKVPEKLLVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDGEIAKQFQRILEKQGFAFKLGAKVTGV
DTSGKTLSARIEPAAGGAAETIEADVVLVAIGRAPYTNGLGLKEAGVALDDRGRVEIDKHFATSVKGIYAIGDVVRGPML
AHKAADEGVACAEILAGQAGHVNYDVIPGVIYTTPEVSSVGKTEEELKQAGTAYAVGKFPFTANGRSKVNQTTDGFVKIL
ADAKTDRVLGVHIIGREAGELIHEACVLMEFGGSAEDLARTCHAHPTRSEAIKEAALAVGKRAIHM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=56.5502183406114, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=28.3549783549784, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI22035672, Length=470, Percent_Identity=30.4255319148936, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI33519430, Length=484, Percent_Identity=29.3388429752066, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519428, Length=484, Percent_Identity=29.3388429752066, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519426, Length=484, Percent_Identity=29.3388429752066, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277071, Length=484, Percent_Identity=29.3388429752066, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI148277065, Length=484, Percent_Identity=29.3388429752066, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI291045266, Length=458, Percent_Identity=28.1659388646288, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI291045268, Length=466, Percent_Identity=28.5407725321888, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786307, Length=452, Percent_Identity=41.5929203539823, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=30.7359307359307, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=28.695652173913, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=30, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=55.2631578947368, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=30.3643724696356, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=25.979381443299, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=26.0770975056689, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=26.0770975056689, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=265, Percent_Identity=25.6603773584906, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=49.6828752642706, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=34.2553191489362, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=29.1220556745182, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=55.2401746724891, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=30.879345603272, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=30.879345603272, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=30.879345603272, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=26.6528925619835, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48823; Mature: 48692

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYDLIIIGTGPGGYVCAVRAAQLGMKVAVVEKNPTLGGTCLNVGCMPSKALLHASEAF
CCEEEEEEEECCCCCEEEEEEHHHCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
QEAGHSFARMGIGVSAPKLDLSAMMDFKQQGIDGNVKGVEYLMKKNKIDVLRGAGRVVAA
HHHCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEECCCCEEEE
GQVEVTGKDGKIQTAETKNIVIATGSDAAKLKGVEIDEKRIVSSTGALSLDKVPEKLLVV
CEEEEECCCCEEEEECCCEEEEEECCCHHHCCCCCCCHHHHHHCCCCEEHHHCCCEEEEE
GAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDGEIAKQFQRILEKQGFAFKLGAKVTG
ECCHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEE
VDTSGKTLSARIEPAAGGAAETIEADVVLVAIGRAPYTNGLGLKEAGVALDDRGRVEIDK
CCCCCCEEEEEECCCCCCCCHHEECCEEEEEECCCCCCCCCCCHHCCCEECCCCCEEEHH
HFATSVKGIYAIGDVVRGPMLAHKAADEGVACAEILAGQAGHVNYDVIPGVIYTTPEVSS
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHCCCCCCCCEEECCCEEEECCCCHH
VGKTEEELKQAGTAYAVGKFPFTANGRSKVNQTTDGFVKILADAKTDRVLGVHIIGREAG
CCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEECCCCCCEEEEEEECCHHH
ELIHEACVLMEFGGSAEDLARTCHAHPTRSEAIKEAALAVGKRAIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTYDLIIIGTGPGGYVCAVRAAQLGMKVAVVEKNPTLGGTCLNVGCMPSKALLHASEAF
CEEEEEEEECCCCCEEEEEEHHHCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
QEAGHSFARMGIGVSAPKLDLSAMMDFKQQGIDGNVKGVEYLMKKNKIDVLRGAGRVVAA
HHHCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEECCCCEEEE
GQVEVTGKDGKIQTAETKNIVIATGSDAAKLKGVEIDEKRIVSSTGALSLDKVPEKLLVV
CEEEEECCCCEEEEECCCEEEEEECCCHHHCCCCCCCHHHHHHCCCCEEHHHCCCEEEEE
GAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDGEIAKQFQRILEKQGFAFKLGAKVTG
ECCHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEE
VDTSGKTLSARIEPAAGGAAETIEADVVLVAIGRAPYTNGLGLKEAGVALDDRGRVEIDK
CCCCCCEEEEEECCCCCCCCHHEECCEEEEEECCCCCCCCCCCHHCCCEECCCCCEEEHH
HFATSVKGIYAIGDVVRGPMLAHKAADEGVACAEILAGQAGHVNYDVIPGVIYTTPEVSS
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHCCCCCCCCEEECCCEEEECCCCHH
VGKTEEELKQAGTAYAVGKFPFTANGRSKVNQTTDGFVKILADAKTDRVLGVHIIGREAG
CCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEECCCCCCEEEEEEECCHHH
ELIHEACVLMEFGGSAEDLARTCHAHPTRSEAIKEAALAVGKRAIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1722146; 2914869 [H]