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The map label for this gene is cobT [H]

Identifier: 75674602

GI number: 75674602

Start: 450614

End: 452521

Strand: Direct

Name: cobT [H]

Synonym: Nwi_0404

Alternate gene names: 75674602

Gene position: 450614-452521 (Clockwise)

Preceding gene: 75674601

Following gene: 75674603

Centisome position: 13.25

GC content: 66.14

Gene sequence:

>1908_bases
ATGAGCACCGCATCACCCAGCAACAGGTTTCGTGCCTCGCCGAAGGAGGCGCCGACAGAGCCGTTCAAGCGCGCGGTCAC
CTCCTGCCTGCGCGCGATCTCCCGGCGGCCGGAGCTTGAGGTAACCTTTGCCATCGAGCGGCCCGGCCTGTCGCCAGGCA
AGGCGCGGCTGCCCGAGCCCGGACGCAAGATGAGCCGGCGGGACGCGGCGATCGTGCGCGGGCATGCGGATTCCATCGCG
CTAAAGCTCGCGTGTCACGATCCGAAGGTGCATCGCAAGCTCATGCCGGGCAATCCGCAGGCGCGCGGCGTCTTCGACGC
GGTCGAGCAGGCCCGCGTCGAGGCCATCGGCTCCCGCCGGATGACGGGCGTCGCCAGGAATCTCACCGCGATGCTCGATG
ATCACTTCCATCGCGGCAAGTATGACGAGATCACCGACCGGGCCGACGCGCCGTTGTCGGATGCGCTGGCGATGATGGTG
CGCGAGCGGCTGACCGGCCTGCCGCCGCCCGCCGCGGCGTCCAGGCTGGTCGACCTGTGGCGCCCGTTCCTGGAAGACAA
GATCGGCGCCCGGCTGGACCAGCTCAGCCATTTTACCGAGGATCAGGCGAAGTTCGGCGACCTCGTGCACGATCTGCTGT
CTGAGCTTGATCTCGGTGACGACAGCCGCGCCGATACGGAGAAGGAGGAGAACGAGGACGACAACCGCGAGGGCGAGAAC
GATCAGTCCGGTACGGAAGGCTCGCCCGACAGCGAGGCCGCCCAGGAGATGAGCGCGGATCAGGCGCAGGACATGACCGA
CGACATGCCTGACGGCGCCATGGAAAGCGCGCAGGCCTCGGTGAGCGACACGTTTGACGATGGCGACCTTGCCGAGGAGG
AGATGCCTGGCGAGGCGACGCGGCCCAATGCGGGCGGCGCCAACGAGCCGCGCGGCCCGGAATATCATGCGTTCGCGCCG
AAGTTCGATGAGGTGATCGCCGCCGAGGATCTGTGCGACCACGATGAACTGGAGCGGCTGCGCAGCTATCTCGACAAGCA
ACTCGCGCACTTGCAGGGCATCGTGGCGCGGCTCGCCAATCGTCTTCAGCGCCGCTTGATGGCGCAACAGAATCGCGCCT
GGGAGTTCGATCTGGAAGAAGGCATTCTCGATCCGGCGCGGCTGTCGCGCGTGGTGATCGACCCTTATCAGCCGCTCTCG
TTCAAGCACGAGAAGGAGGCGACGTTCCGCGATACCGTGGTGACGCTGCTGCTCGACAATTCCGGTTCGATGCGGGGGCG
GCCGATCACGGTGGCCGCGACCTGCGCCGACATTCTGGCGCGCACGCTGGAACGCTGCGGCGTCAAGGTCGAAATTCTCG
GCTTCACGACGCGGGCCTGGAAGGGCGGGCAGTCGCGCGAGGCGTGGCTCGCCGCCGGCAAGCCGGTCACGCCGGGGCGG
CTCAACGATCTCCGCCATATCATTTACAAGGCGGCCGACGCTCCCTGGCGGCGATCGCGGAAAAACCTCGGACTGATGAT
GCGCGAGGGCCTGCTCAAGGAAAACATCGACGGCGAGGCGCTCGACTGGGCGCATAAACGGCTGCTGGGCCGTTCCGAGC
AGCGCAAGATCCTGATGATGATTTCGGACGGTGCGCCGGTCGACGATTCGACCCTGTCGGTCAATCCGGGAAATTATCTG
GAGCGTCACCTGCGATGGGTGATCGAGGAAATCGAAACCAGATCCCCCGTCGAACTGATCGCCATCGGCATCGGTCACGA
CGTGACGCGCTACTATCGCCGCGCGGTGACCATCGTGGATGCCGAGGAACTCGGCGGCGCCATGACCGAACAACTCGCCG
AACTGTTCAGCGAGACCCATGAGCCGCCGCCAGGTCCGGCACGGCGGCCACGCAAGCTGCATTCGTAG

Upstream 100 bases:

>100_bases
GTTCTGCAAACCCGGCGGCGCACCAGCGCGCCTGAGCGAGCCGGGCGAGGGATTTTTTTTTGGCTGTTCGCGCCCATAGG
GGACCACCGTACAGGTTTTC

Downstream 100 bases:

>100_bases
CCGGCAGAACCTGTTGCATTCCGGGCGGGATATCGTCCTGCGTATGTGACGCGTCCCCGGCAGATTGGTTTCTCCGCAAG
ATGGCATCATGGATCGATCG

Product: cobalt chelatase, CobT subunit

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MSTASPSNRFRASPKEAPTEPFKRAVTSCLRAISRRPELEVTFAIERPGLSPGKARLPEPGRKMSRRDAAIVRGHADSIA
LKLACHDPKVHRKLMPGNPQARGVFDAVEQARVEAIGSRRMTGVARNLTAMLDDHFHRGKYDEITDRADAPLSDALAMMV
RERLTGLPPPAAASRLVDLWRPFLEDKIGARLDQLSHFTEDQAKFGDLVHDLLSELDLGDDSRADTEKEENEDDNREGEN
DQSGTEGSPDSEAAQEMSADQAQDMTDDMPDGAMESAQASVSDTFDDGDLAEEEMPGEATRPNAGGANEPRGPEYHAFAP
KFDEVIAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWEFDLEEGILDPARLSRVVIDPYQPLS
FKHEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPVTPGR
LNDLRHIIYKAADAPWRRSRKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYL
ERHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAMTEQLAELFSETHEPPPGPARRPRKLHS

Sequences:

>Translated_635_residues
MSTASPSNRFRASPKEAPTEPFKRAVTSCLRAISRRPELEVTFAIERPGLSPGKARLPEPGRKMSRRDAAIVRGHADSIA
LKLACHDPKVHRKLMPGNPQARGVFDAVEQARVEAIGSRRMTGVARNLTAMLDDHFHRGKYDEITDRADAPLSDALAMMV
RERLTGLPPPAAASRLVDLWRPFLEDKIGARLDQLSHFTEDQAKFGDLVHDLLSELDLGDDSRADTEKEENEDDNREGEN
DQSGTEGSPDSEAAQEMSADQAQDMTDDMPDGAMESAQASVSDTFDDGDLAEEEMPGEATRPNAGGANEPRGPEYHAFAP
KFDEVIAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWEFDLEEGILDPARLSRVVIDPYQPLS
FKHEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPVTPGR
LNDLRHIIYKAADAPWRRSRKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYL
ERHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAMTEQLAELFSETHEPPPGPARRPRKLHS
>Mature_634_residues
STASPSNRFRASPKEAPTEPFKRAVTSCLRAISRRPELEVTFAIERPGLSPGKARLPEPGRKMSRRDAAIVRGHADSIAL
KLACHDPKVHRKLMPGNPQARGVFDAVEQARVEAIGSRRMTGVARNLTAMLDDHFHRGKYDEITDRADAPLSDALAMMVR
ERLTGLPPPAAASRLVDLWRPFLEDKIGARLDQLSHFTEDQAKFGDLVHDLLSELDLGDDSRADTEKEENEDDNREGEND
QSGTEGSPDSEAAQEMSADQAQDMTDDMPDGAMESAQASVSDTFDDGDLAEEEMPGEATRPNAGGANEPRGPEYHAFAPK
FDEVIAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWEFDLEEGILDPARLSRVVIDPYQPLSF
KHEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPVTPGRL
NDLRHIIYKAADAPWRRSRKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYLE
RHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAMTEQLAELFSETHEPPPGPARRPRKLHS

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 70703; Mature: 70572

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50234 VWFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTASPSNRFRASPKEAPTEPFKRAVTSCLRAISRRPELEVTFAIERPGLSPGKARLPEP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCC
GRKMSRRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGVFDAVEQARVEAIGSRR
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MTGVARNLTAMLDDHFHRGKYDEITDRADAPLSDALAMMVRERLTGLPPPAAASRLVDLW
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RPFLEDKIGARLDQLSHFTEDQAKFGDLVHDLLSELDLGDDSRADTEKEENEDDNREGEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
DQSGTEGSPDSEAAQEMSADQAQDMTDDMPDGAMESAQASVSDTFDDGDLAEEEMPGEAT
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
RPNAGGANEPRGPEYHAFAPKFDEVIAAEDLCDHDELERLRSYLDKQLAHLQGIVARLAN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQRRLMAQQNRAWEFDLEEGILDPARLSRVVIDPYQPLSFKHEKEATFRDTVVTLLLDN
HHHHHHHHHHCCCEECCHHHCCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHEEEEEECC
SGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPVTPGR
CCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCHHHCCCCCCCCCH
LNDLRHIIYKAADAPWRRSRKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMM
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEE
ISDGAPVDDSTLSVNPGNYLERHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVD
EECCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHEEE
AEELGGAMTEQLAELFSETHEPPPGPARRPRKLHS
HHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STASPSNRFRASPKEAPTEPFKRAVTSCLRAISRRPELEVTFAIERPGLSPGKARLPEP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCC
GRKMSRRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGVFDAVEQARVEAIGSRR
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MTGVARNLTAMLDDHFHRGKYDEITDRADAPLSDALAMMVRERLTGLPPPAAASRLVDLW
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RPFLEDKIGARLDQLSHFTEDQAKFGDLVHDLLSELDLGDDSRADTEKEENEDDNREGEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
DQSGTEGSPDSEAAQEMSADQAQDMTDDMPDGAMESAQASVSDTFDDGDLAEEEMPGEAT
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
RPNAGGANEPRGPEYHAFAPKFDEVIAAEDLCDHDELERLRSYLDKQLAHLQGIVARLAN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQRRLMAQQNRAWEFDLEEGILDPARLSRVVIDPYQPLSFKHEKEATFRDTVVTLLLDN
HHHHHHHHHHCCCEECCHHHCCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHEEEEEECC
SGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPVTPGR
CCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCHHHCCCCCCCCCH
LNDLRHIIYKAADAPWRRSRKNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMM
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEE
ISDGAPVDDSTLSVNPGNYLERHLRWVIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVD
EECCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHEEE
AEELGGAMTEQLAELFSETHEPPPGPARRPRKLHS
HHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]