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The map label for this gene is bla [H]

Identifier: 75674549

GI number: 75674549

Start: 392871

End: 393761

Strand: Direct

Name: bla [H]

Synonym: Nwi_0351

Alternate gene names: 75674549

Gene position: 392871-393761 (Clockwise)

Preceding gene: 75674548

Following gene: 75674557

Centisome position: 11.55

GC content: 66.11

Gene sequence:

>891_bases
ATGATCAGCAGACGAGGTTTGTGGCCTGTCGGTGCGGGGCTGGCCGGGCTTGTCGTCATGGGCCGGGCCGGCTTTGCGGT
TGCCCCCGCCGCATTGCAGGACAGGTTTGTCGAGGAACTGAAACGGTTGGAAAATCAAACAGGCGGGAGACTGGGCGTCG
CACTGCTCGATGCCGGCACCGGCCAGCACGTCGGCCATCGAATGGATGAGCCATTCCCGATGTGCAGCACGTTCAAGGTG
CTGGCGGCGGGCGCCCTTCTTCAGGGCGTCGATGCTGGCAAGGAGGATCTCGCGCGGCGAATCTATTTCAATGAGGCCGA
TCTGGTGGCCCATTCGCCGGAGACTCGCAAACACGTCGGTCCGACCGGCATGACCGTTGCCGAACTGTGCAAGGCCGCGA
TAACGCTCAGCGACAACACCGCGGGCAATCTGCTGCTTGCGAGTATCGGCGGTCCACAGGGGCTGACGGCCTTCGCACGA
AGGCTTGGCGACGAGGTGACGCGCCTCGACCGGATCGAGACCGAACTCAATGAGTCGGTGCCGGGCGATCCGCGCGACAC
GACCACGCCGAGTGCGATGGCCGCAAACCTGCGAGCGCTCGCGCTGGGCGATGTGCTGTCGGTGAAATCACGCACGCAAC
TTGTGGGCTGGCTGGTCGCAAATACCACCGGCGACAAGCGGCTGTGCGCCGGATTGCCGGCCGGCTGGCGGGCCGGCGAC
AAGACCGGAACCGGCGAGCGGGGGACTGCGAACGATGTCGCCGTGATCTGGCCGCCCGACCGCGCGCCCTTCGTCGTAAC
GGCCTATCTCACCGGCGCCACCGTCCCGGCGGATCGGCAGAATGCGGTCATGGCAGCCATCGGACGCGCGGTCACGACCG
TGTTGGCCTGA

Upstream 100 bases:

>100_bases
CAGAACTTCACCAAATTCGAGAACGACGTCGATGCCGAGGTCCGCGAGGCGGCGCCCGGCACGATGATGGCGGCAGAGTA
ATGTTGGGAACGATGAAGGC

Downstream 100 bases:

>100_bases
TTTTCGCGACCTGCGCGGGCCTCGGCTCGCTGAGGGTGTTGCTCGGGGTCGGACGATCGGTGATCTGCTGCCCGAACAGG
CTGGCGAGCAATTCCACCGC

Product: beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MISRRGLWPVGAGLAGLVVMGRAGFAVAPAALQDRFVEELKRLENQTGGRLGVALLDAGTGQHVGHRMDEPFPMCSTFKV
LAAGALLQGVDAGKEDLARRIYFNEADLVAHSPETRKHVGPTGMTVAELCKAAITLSDNTAGNLLLASIGGPQGLTAFAR
RLGDEVTRLDRIETELNESVPGDPRDTTTPSAMAANLRALALGDVLSVKSRTQLVGWLVANTTGDKRLCAGLPAGWRAGD
KTGTGERGTANDVAVIWPPDRAPFVVTAYLTGATVPADRQNAVMAAIGRAVTTVLA

Sequences:

>Translated_296_residues
MISRRGLWPVGAGLAGLVVMGRAGFAVAPAALQDRFVEELKRLENQTGGRLGVALLDAGTGQHVGHRMDEPFPMCSTFKV
LAAGALLQGVDAGKEDLARRIYFNEADLVAHSPETRKHVGPTGMTVAELCKAAITLSDNTAGNLLLASIGGPQGLTAFAR
RLGDEVTRLDRIETELNESVPGDPRDTTTPSAMAANLRALALGDVLSVKSRTQLVGWLVANTTGDKRLCAGLPAGWRAGD
KTGTGERGTANDVAVIWPPDRAPFVVTAYLTGATVPADRQNAVMAAIGRAVTTVLA
>Mature_296_residues
MISRRGLWPVGAGLAGLVVMGRAGFAVAPAALQDRFVEELKRLENQTGGRLGVALLDAGTGQHVGHRMDEPFPMCSTFKV
LAAGALLQGVDAGKEDLARRIYFNEADLVAHSPETRKHVGPTGMTVAELCKAAITLSDNTAGNLLLASIGGPQGLTAFAR
RLGDEVTRLDRIETELNESVPGDPRDTTTPSAMAANLRALALGDVLSVKSRTQLVGWLVANTTGDKRLCAGLPAGWRAGD
KTGTGERGTANDVAVIWPPDRAPFVVTAYLTGATVPADRQNAVMAAIGRAVTTVLA

Specific function: Confers high levels of resitance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam [H]

COG id: COG2367

COG function: function code V; Beta-lactamase class A

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 30941; Mature: 30941

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRRGLWPVGAGLAGLVVMGRAGFAVAPAALQDRFVEELKRLENQTGGRLGVALLDAGT
CCCCCCCCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GQHVGHRMDEPFPMCSTFKVLAAGALLQGVDAGKEDLARRIYFNEADLVAHSPETRKHVG
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHEECCCCHHHHCC
PTGMTVAELCKAAITLSDNTAGNLLLASIGGPQGLTAFARRLGDEVTRLDRIETELNESV
CCCCHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGDPRDTTTPSAMAANLRALALGDVLSVKSRTQLVGWLVANTTGDKRLCAGLPAGWRAGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHCCCCCCCCCC
KTGTGERGTANDVAVIWPPDRAPFVVTAYLTGATVPADRQNAVMAAIGRAVTTVLA
CCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISRRGLWPVGAGLAGLVVMGRAGFAVAPAALQDRFVEELKRLENQTGGRLGVALLDAGT
CCCCCCCCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GQHVGHRMDEPFPMCSTFKVLAAGALLQGVDAGKEDLARRIYFNEADLVAHSPETRKHVG
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHEECCCCHHHHCC
PTGMTVAELCKAAITLSDNTAGNLLLASIGGPQGLTAFARRLGDEVTRLDRIETELNESV
CCCCHHHHHHHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGDPRDTTTPSAMAANLRALALGDVLSVKSRTQLVGWLVANTTGDKRLCAGLPAGWRAGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHCCCCCCCCCC
KTGTGERGTANDVAVIWPPDRAPFVVTAYLTGATVPADRQNAVMAAIGRAVTTVLA
CCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11181374 [H]