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The map label for this gene is pckA

Identifier: 75674548

GI number: 75674548

Start: 391235

End: 392851

Strand: Direct

Name: pckA

Synonym: Nwi_0350

Alternate gene names: 75674548

Gene position: 391235-392851 (Clockwise)

Preceding gene: 75674547

Following gene: 75674549

Centisome position: 11.5

GC content: 62.28

Gene sequence:

>1617_bases
GTGCCTGAGATAGGCGTGCGTAACGGCGCCTTCGGCGCCGATAAGTTTGGCCTCCAGAATCTCAAGGCCGTGTACTGGAA
TCCAGGCGCGCCGCAGCTTTATGAGCACGCACTGCGCTCGGGCGAAGCGATGGTGAATGCGGATGGCGCGCTATGCGCGG
AAACCGGCATCTTTACCGGCCGCAGCCCGAAGGACAAATTCACGGTCCGCGATGCGGCCACGGATAAGAGTGTCTGGTGG
GCCGGCAACCAGTCGATGACGCCTGACCAGTTCGCCGCGTTGTATTCCGACTTTCTCGCGCACGCCCGGAACATGACGCT
GTTCGCGCAGGACCTCTATGGCGGCGCCGACCCCAATTTCCGTATCAAGACCCGCGTTTTCACCGAACTGGCCTGGCACT
CGCTTTTTATTCGCACCCTGCTGCGCCGGCCGGAAACATCGGAACTTGCCGCCTTCGTACCTGAACTGACCGTCATCGAC
CTGCCGAGCTTCCGCGCCGATCCCCAAAGGCACGGCGTTCGCTCCGAGAACGTCGTGGCCATCGATTTCACACGCAGGAT
CATTCTCATCGGCGGATCGCATTACGCCGGGGAGATGAAGAAAAGCATCTTCACCACGCTGAATTATTACCTTCCCGATC
AGGACGTGTTGCCGATGCACTGTTCGGCCAATGTCGGTCCCGGCGGCGACAGCGCGATTTTCTTCGGACTGTCGGGTACG
GGTAAAACCACGCTGTCGGCTGATCCTGGGCGCACGCTGATCGGCGACGACGAGCACGGCTGGAGCAAGGACGGCATCTT
CAATTTCGAAGGGGGATGCTACGCCAAATGCATCCGGCTGTCGCGGGAGGCCGAGCCCGAGATTTACGCCGCCTCGAAAC
GCTTCGGCGCGGTGCTTGAGAATGTCGTGCACGACGAAGTCACGCGCGTCCCCGATTTCAACGACGGCTCGAAGACCGAG
AACACCCGTTCGGCCTATCCGCTCGAATTCATCCCGAACGCGTCGCTGACAGGTTGCGCCGGCCAGCCGAAGAATCTGGT
CATGCTGGCGGCCGACGCTTTCGGCGTGCTGCCGCCCATCGCCAGGCTGACGCCGGCGCAGGCCATGTATCATTTTCTGT
CGGGCTATACGGCAAAGGTGGCGGGAACCGAGCGCGACCTTGGCAATGAGCCGCAGCCGGAATTTTCCGCCTGTTTTGGC
TCGCCGTTCCTGCCGCGCCATCCCGGCGTCTACGGCAACATGCTGCGTGCCCTGATCGCGAAGCACAACGTCGATTGCTG
GCTGGTGAACACCGGCTGGACCGGGGGCAAATACGGGACCGGCCGCAGAATGCCGATCAAGGTGACGCGTGCCCTGCTAG
GCGCCGCGCTTGACGGCTCCTTGCGCGGCGTTCCATTCCACGATGACAGGTATTTCGGATTCGCGGTTCCGACCTCGGTG
CCCGGCGTCGAGCCGCATATCCTTGATCCGATCAGGACCTGGGCCGACAAGATCGAGTTCGACAGGACGGCGCGCGCGCT
GATCGGCATGTTCCGGCAGAACTTCACCAAATTCGAGAACGACGTCGATGCCGAGGTCCGCGAGGCGGCGCCCGGCACGA
TGATGGCGGCAGAGTAA

Upstream 100 bases:

>100_bases
TTTGACGCGTTTTCTTCACGCGAACCGGTGCCCACTTCGCTTGAAAACGCTCTAGGCTCGATCCGGAAGTCGCCCTGGAT
TCGACGAGGAGGAATTTTCC

Downstream 100 bases:

>100_bases
TGTTGGGAACGATGAAGGCATGATCAGCAGACGAGGTTTGTGGCCTGTCGGTGCGGGGCTGGCCGGGCTTGTCGTCATGG
GCCGGGCCGGCTTTGCGGTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MPEIGVRNGAFGADKFGLQNLKAVYWNPGAPQLYEHALRSGEAMVNADGALCAETGIFTGRSPKDKFTVRDAATDKSVWW
AGNQSMTPDQFAALYSDFLAHARNMTLFAQDLYGGADPNFRIKTRVFTELAWHSLFIRTLLRRPETSELAAFVPELTVID
LPSFRADPQRHGVRSENVVAIDFTRRIILIGGSHYAGEMKKSIFTTLNYYLPDQDVLPMHCSANVGPGGDSAIFFGLSGT
GKTTLSADPGRTLIGDDEHGWSKDGIFNFEGGCYAKCIRLSREAEPEIYAASKRFGAVLENVVHDEVTRVPDFNDGSKTE
NTRSAYPLEFIPNASLTGCAGQPKNLVMLAADAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTERDLGNEPQPEFSACFG
SPFLPRHPGVYGNMLRALIAKHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLRGVPFHDDRYFGFAVPTSV
PGVEPHILDPIRTWADKIEFDRTARALIGMFRQNFTKFENDVDAEVREAAPGTMMAAE

Sequences:

>Translated_538_residues
MPEIGVRNGAFGADKFGLQNLKAVYWNPGAPQLYEHALRSGEAMVNADGALCAETGIFTGRSPKDKFTVRDAATDKSVWW
AGNQSMTPDQFAALYSDFLAHARNMTLFAQDLYGGADPNFRIKTRVFTELAWHSLFIRTLLRRPETSELAAFVPELTVID
LPSFRADPQRHGVRSENVVAIDFTRRIILIGGSHYAGEMKKSIFTTLNYYLPDQDVLPMHCSANVGPGGDSAIFFGLSGT
GKTTLSADPGRTLIGDDEHGWSKDGIFNFEGGCYAKCIRLSREAEPEIYAASKRFGAVLENVVHDEVTRVPDFNDGSKTE
NTRSAYPLEFIPNASLTGCAGQPKNLVMLAADAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTERDLGNEPQPEFSACFG
SPFLPRHPGVYGNMLRALIAKHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLRGVPFHDDRYFGFAVPTSV
PGVEPHILDPIRTWADKIEFDRTARALIGMFRQNFTKFENDVDAEVREAAPGTMMAAE
>Mature_537_residues
PEIGVRNGAFGADKFGLQNLKAVYWNPGAPQLYEHALRSGEAMVNADGALCAETGIFTGRSPKDKFTVRDAATDKSVWWA
GNQSMTPDQFAALYSDFLAHARNMTLFAQDLYGGADPNFRIKTRVFTELAWHSLFIRTLLRRPETSELAAFVPELTVIDL
PSFRADPQRHGVRSENVVAIDFTRRIILIGGSHYAGEMKKSIFTTLNYYLPDQDVLPMHCSANVGPGGDSAIFFGLSGTG
KTTLSADPGRTLIGDDEHGWSKDGIFNFEGGCYAKCIRLSREAEPEIYAASKRFGAVLENVVHDEVTRVPDFNDGSKTEN
TRSAYPLEFIPNASLTGCAGQPKNLVMLAADAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTERDLGNEPQPEFSACFGS
PFLPRHPGVYGNMLRALIAKHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLRGVPFHDDRYFGFAVPTSVP
GVEPHILDPIRTWADKIEFDRTARALIGMFRQNFTKFENDVDAEVREAAPGTMMAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=522, Percent_Identity=46.1685823754789, Blast_Score=451, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322950, Length=515, Percent_Identity=44.8543689320388, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_NITWN (Q3SVS4)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316969.1
- ProteinModelPortal:   Q3SVS4
- SMR:   Q3SVS4
- STRING:   Q3SVS4
- GeneID:   3674904
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0350
- NMPDR:   fig|323098.3.peg.819
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   Q3SVS4
- ProtClustDB:   PRK09344
- BioCyc:   NWIN323098:NWI_0350-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58963; Mature: 58832

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00532 PEPCK_ATP; PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIGVRNGAFGADKFGLQNLKAVYWNPGAPQLYEHALRSGEAMVNADGALCAETGIFTG
CCCCCCCCCCCCCCHHCCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCEEECCCCCCC
RSPKDKFTVRDAATDKSVWWAGNQSMTPDQFAALYSDFLAHARNMTLFAQDLYGGADPNF
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCE
RIKTRVFTELAWHSLFIRTLLRRPETSELAAFVPELTVIDLPSFRADPQRHGVRSENVVA
EEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCEEE
IDFTRRIILIGGSHYAGEMKKSIFTTLNYYLPDQDVLPMHCSANVGPGGDSAIFFGLSGT
EEECCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEECCC
GKTTLSADPGRTLIGDDEHGWSKDGIFNFEGGCYAKCIRLSREAEPEIYAASKRFGAVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHH
NVVHDEVTRVPDFNDGSKTENTRSAYPLEFIPNASLTGCAGQPKNLVMLAADAFGVLPPI
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCHHCCCCCH
ARLTPAQAMYHFLSGYTAKVAGTERDLGNEPQPEFSACFGSPFLPRHPGVYGNMLRALIA
HHCCHHHHHHHHHCCCEEEECCCHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHH
KHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLRGVPFHDDRYFGFAVPTSV
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
PGVEPHILDPIRTWADKIEFDRTARALIGMFRQNFTKFENDVDAEVREAAPGTMMAAE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEECC
>Mature Secondary Structure 
PEIGVRNGAFGADKFGLQNLKAVYWNPGAPQLYEHALRSGEAMVNADGALCAETGIFTG
CCCCCCCCCCCCCHHCCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCEEECCCCCCC
RSPKDKFTVRDAATDKSVWWAGNQSMTPDQFAALYSDFLAHARNMTLFAQDLYGGADPNF
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCE
RIKTRVFTELAWHSLFIRTLLRRPETSELAAFVPELTVIDLPSFRADPQRHGVRSENVVA
EEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCEEE
IDFTRRIILIGGSHYAGEMKKSIFTTLNYYLPDQDVLPMHCSANVGPGGDSAIFFGLSGT
EEECCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEECCC
GKTTLSADPGRTLIGDDEHGWSKDGIFNFEGGCYAKCIRLSREAEPEIYAASKRFGAVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHH
NVVHDEVTRVPDFNDGSKTENTRSAYPLEFIPNASLTGCAGQPKNLVMLAADAFGVLPPI
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCHHCCCCCH
ARLTPAQAMYHFLSGYTAKVAGTERDLGNEPQPEFSACFGSPFLPRHPGVYGNMLRALIA
HHCCHHHHHHHHHCCCEEEECCCHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHH
KHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLRGVPFHDDRYFGFAVPTSV
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
PGVEPHILDPIRTWADKIEFDRTARALIGMFRQNFTKFENDVDAEVREAAPGTMMAAE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA