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The map label for this gene is thrC [H]

Identifier: 75674428

GI number: 75674428

Start: 259851

End: 261269

Strand: Direct

Name: thrC [H]

Synonym: Nwi_0229

Alternate gene names: 75674428

Gene position: 259851-261269 (Clockwise)

Preceding gene: 75674427

Following gene: 75674429

Centisome position: 7.64

GC content: 63.64

Gene sequence:

>1419_bases
TTGACGCACTATCTTTCGACACGGGGAGAGAATCAAAAACTCGGTTTCTCTGATGTGATGCTGGCCGGACTCGCACGGGA
CGGCGGTCTCTACGTGCCGGAGGTCTGGCCGCAATTGCGGCTCGATACGGTTGCCGCCTTTTTCGGCAGGCCCTACTCGG
AGGTAGCCGTTGAGGTGATCCGGCCGTTCGTGGGCGGAGATATCGCCGACGTCGACCTCGCTCGCATGGCCAACGAGGCT
TATGCGACCTTCCGGCACCCCGCGGTGGTCCCGCTCGATCAGATAGGCTCTCAACAGTTCCTGCTGGAACTGTTTCACGG
TCCGACGCTGGCGTTCAAGGACGTGGCGATGCAGTTGCTGTCGCGGCTGATGGACCACGTTCTGGCGAGACGCGGCCAGC
GCACCACCATCGTCGTGGCCACCTCGGGTGACACCGGCGGCGCGGCAGTGGATGCATTCGCCAACCGTGCGAATGTTGAT
CTGATCGCCTTGTTCCCGGACGGCCGCATTTCCGAGGTGCAGCGACGCATGATGACGACGACGGCCGCGCCGAATGTTCA
TGCGCTTGCGGTCAAGGGCACGTTCGACGACTGCCAGGCGATCGTGAAGGGCCTGTTCAACAATCACCGCTTCCGCGACG
AGGTATCGCTCTCGGGAGTCAACTCGATCAACTGGGCGCGGATCGTTGCACAGGTAGTGTATTACTTCACCTCTGCGGTC
GCGCTCGGCGCGCCCGCGCGCGCCGTTGATTTTACCGTGCCGACCGGTAATTTCGGCGACATCTTCGCCGGTTACGTCGC
AAAGCGCATGGGACTGCCGGTACGCCGGCTGCGGATCGCCGCCAACATCAATGATATTCTGCCGCGCACGCTGAAGACCG
GAATTTATGAGGTGCGCGAGGTTCACGCCACCTCTTCGCCGTCGATGGACATTCAGGTGTCGTCGAACTTCGAACGGCTG
TTGTTCGAGGCGACCGGGCGTGATGCCGCGCAGGTGCGTGCGCTGATGGCCTCGTTGCAGCAGTCCGGCCGTTTCGCGCT
ACCGGAGTCGGTTCTGGCCGCGATGCGCGCGGATTTCGATGCCGAATGTGCCGACGAAACGGAGACCGCGGCCACGATCC
GCACGGCGTGGCGCGAAGCCGGAGATCTGCTCGACCCGCATACGGCTGTTGCCTTGACGGTGGCCGATCGTGACGCGGCG
GAGTCGCGCGTTCCCAACATCGTGCTCGCCACCGCCCATGCCGCCAAGTTCCCTGACGCCGTTGAGGCGGCCTGCGGCGT
ACGGCCGCCGTTGCCGGCCTGGCTCGACGGCTTGATGACCCGGCCCGAGCGGATCACGATCGTGAACAACGATCAGGCAG
AAATCGAACGGTTCGTGCTGTCCGTCAGCCGCGCCGCCAATCACGGAGTTACCGGATGA

Upstream 100 bases:

>100_bases
ATTTCAAGAGGCTTTATGATACGCAGCGCCTGTCACCGACATTGACGGCGGCTCGTGGCATCAGACGTAAGGCAACAAAC
TTCGGCGCTTGGGGGCCGGC

Downstream 100 bases:

>100_bases
GTGTCAATGTCACGAAGCTGTCGTCCGGCCTCACCGTCGTGACTGATGACATGCCACATCTGGAAACCGCCGCGCTCGGT
GTCTGGACCGGAGTCGGCGG

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTHYLSTRGENQKLGFSDVMLAGLARDGGLYVPEVWPQLRLDTVAAFFGRPYSEVAVEVIRPFVGGDIADVDLARMANEA
YATFRHPAVVPLDQIGSQQFLLELFHGPTLAFKDVAMQLLSRLMDHVLARRGQRTTIVVATSGDTGGAAVDAFANRANVD
LIALFPDGRISEVQRRMMTTTAAPNVHALAVKGTFDDCQAIVKGLFNNHRFRDEVSLSGVNSINWARIVAQVVYYFTSAV
ALGAPARAVDFTVPTGNFGDIFAGYVAKRMGLPVRRLRIAANINDILPRTLKTGIYEVREVHATSSPSMDIQVSSNFERL
LFEATGRDAAQVRALMASLQQSGRFALPESVLAAMRADFDAECADETETAATIRTAWREAGDLLDPHTAVALTVADRDAA
ESRVPNIVLATAHAAKFPDAVEAACGVRPPLPAWLDGLMTRPERITIVNNDQAEIERFVLSVSRAANHGVTG

Sequences:

>Translated_472_residues
MTHYLSTRGENQKLGFSDVMLAGLARDGGLYVPEVWPQLRLDTVAAFFGRPYSEVAVEVIRPFVGGDIADVDLARMANEA
YATFRHPAVVPLDQIGSQQFLLELFHGPTLAFKDVAMQLLSRLMDHVLARRGQRTTIVVATSGDTGGAAVDAFANRANVD
LIALFPDGRISEVQRRMMTTTAAPNVHALAVKGTFDDCQAIVKGLFNNHRFRDEVSLSGVNSINWARIVAQVVYYFTSAV
ALGAPARAVDFTVPTGNFGDIFAGYVAKRMGLPVRRLRIAANINDILPRTLKTGIYEVREVHATSSPSMDIQVSSNFERL
LFEATGRDAAQVRALMASLQQSGRFALPESVLAAMRADFDAECADETETAATIRTAWREAGDLLDPHTAVALTVADRDAA
ESRVPNIVLATAHAAKFPDAVEAACGVRPPLPAWLDGLMTRPERITIVNNDQAEIERFVLSVSRAANHGVTG
>Mature_471_residues
THYLSTRGENQKLGFSDVMLAGLARDGGLYVPEVWPQLRLDTVAAFFGRPYSEVAVEVIRPFVGGDIADVDLARMANEAY
ATFRHPAVVPLDQIGSQQFLLELFHGPTLAFKDVAMQLLSRLMDHVLARRGQRTTIVVATSGDTGGAAVDAFANRANVDL
IALFPDGRISEVQRRMMTTTAAPNVHALAVKGTFDDCQAIVKGLFNNHRFRDEVSLSGVNSINWARIVAQVVYYFTSAVA
LGAPARAVDFTVPTGNFGDIFAGYVAKRMGLPVRRLRIAANINDILPRTLKTGIYEVREVHATSSPSMDIQVSSNFERLL
FEATGRDAAQVRALMASLQQSGRFALPESVLAAMRADFDAECADETETAATIRTAWREAGDLLDPHTAVALTVADRDAAE
SRVPNIVLATAHAAKFPDAVEAACGVRPPLPAWLDGLMTRPERITIVNNDQAEIERFVLSVSRAANHGVTG

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=454, Percent_Identity=34.8017621145374, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI153792148, Length=437, Percent_Identity=35.2402745995423, Blast_Score=260, Evalue=2e-69,
Organism=Escherichia coli, GI1786185, Length=431, Percent_Identity=33.1786542923434, Blast_Score=214, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6319901, Length=456, Percent_Identity=41.4473684210526, Blast_Score=327, Evalue=2e-90,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 51189; Mature: 51058

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHYLSTRGENQKLGFSDVMLAGLARDGGLYVPEVWPQLRLDTVAAFFGRPYSEVAVEVI
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCHHHHHHHHH
RPFVGGDIADVDLARMANEAYATFRHPAVVPLDQIGSQQFLLELFHGPTLAFKDVAMQLL
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
SRLMDHVLARRGQRTTIVVATSGDTGGAAVDAFANRANVDLIALFPDGRISEVQRRMMTT
HHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
TAAPNVHALAVKGTFDDCQAIVKGLFNNHRFRDEVSLSGVNSINWARIVAQVVYYFTSAV
CCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALGAPARAVDFTVPTGNFGDIFAGYVAKRMGLPVRRLRIAANINDILPRTLKTGIYEVRE
HCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
VHATSSPSMDIQVSSNFERLLFEATGRDAAQVRALMASLQQSGRFALPESVLAAMRADFD
HHCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
AECADETETAATIRTAWREAGDLLDPHTAVALTVADRDAAESRVPNIVLATAHAAKFPDA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHCCEEEEEECHHHCCCHH
VEAACGVRPPLPAWLDGLMTRPERITIVNNDQAEIERFVLSVSRAANHGVTG
HHHHCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
THYLSTRGENQKLGFSDVMLAGLARDGGLYVPEVWPQLRLDTVAAFFGRPYSEVAVEVI
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCHHHHHHHHH
RPFVGGDIADVDLARMANEAYATFRHPAVVPLDQIGSQQFLLELFHGPTLAFKDVAMQLL
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
SRLMDHVLARRGQRTTIVVATSGDTGGAAVDAFANRANVDLIALFPDGRISEVQRRMMTT
HHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
TAAPNVHALAVKGTFDDCQAIVKGLFNNHRFRDEVSLSGVNSINWARIVAQVVYYFTSAV
CCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALGAPARAVDFTVPTGNFGDIFAGYVAKRMGLPVRRLRIAANINDILPRTLKTGIYEVRE
HCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
VHATSSPSMDIQVSSNFERLLFEATGRDAAQVRALMASLQQSGRFALPESVLAAMRADFD
HHCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
AECADETETAATIRTAWREAGDLLDPHTAVALTVADRDAAESRVPNIVLATAHAAKFPDA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHCCEEEEEECHHHCCCHH
VEAACGVRPPLPAWLDGLMTRPERITIVNNDQAEIERFVLSVSRAANHGVTG
HHHHCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1333566; 10984043 [H]