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The map label for this gene is ydaM [H]

Identifier: 74318832

GI number: 74318832

Start: 2895176

End: 2896141

Strand: Direct

Name: ydaM [H]

Synonym: Tbd_2814

Alternate gene names: 74318832

Gene position: 2895176-2896141 (Clockwise)

Preceding gene: 74318827

Following gene: 74318835

Centisome position: 99.5

GC content: 67.18

Gene sequence:

>966_bases
ATGGAAATCTACCAGCGCATTTTCGAATACGCGCCCGACGCGATGCTCGTGCTCGGCCGCGACGGCCTCCTGCGCCTGGT
CAACGCGCACGCCGAGACGCTGTTCGGCTACGACCGCAGCGAACTGCTCGGCCAGACCGTCGAGCTGCTCGTGCCCGCGC
GTTTCACGGCGCAGCACGCGGCGCACCGCGCGCGCTTCATGGAGGAGGCGCAGAGCCGGCAGATGGGGCGCCAGCAGGAT
CTTTACGCACTGCGCAAGGACGGCAGCGAGTTCCCGGTCGACATCATGCTGAGCCCGATGATCATGGGCGGCGAGCACCT
GACGCTATGCGTGGTGCGCGACATCACCGAACGCAAGGCCGCGCAGGATGCGCTCGAGCATCAGACCAACGAGTTGTTGC
GCCTGCATGCCGAACTCGAACTCCTCGCCAACCACGACGGGCTCACCGGCCTGTACAACTGGCGGGCGTTCTATGAACAC
GCCGGACAGTTGCTGACGACCGCGCACCGCCGCCACGAGAAAGCGTCGCTCCTGATCCTCGACCTCGATCATTTCAAGCA
GATCAACGACCGCTTCGGCCACGCCGAAGGCGACCGCGTGCTGCAAGCCGCCGCCGCCGCGTTGAAGGCGAGCGCGCGGC
AGAACGACATCGTCGCCCGGCACGGCGGCGAAGAGTTCGTGATCGCCGGGTTCGGCCTCAGCGAAGCGGAAAGCCTCGTT
GCCGCCGAGCGCCTGCGCGCGGCGGTCGCGAGCATCGAAAATCTCGCGTGCCGCGTGACGACGAGCGTCGGCGTCGCCAC
CTTCGTGCCCGACCCGTCGAGCAGGCACGAACCGCCGACGCTACTGGCAGATCTGCTCGATCGGGCCGATCAGGCGCTCT
ACCACGCCAAGCGGAGCGGCAGGAACCGGATCTGCCACTTCGACCAGCTCGGGGAAGACATCGCGCCGGCGAGCGATTCA
CCGTAG

Upstream 100 bases:

>100_bases
AAATGAGGGTCCCGGCGACGCGGCCGACCATGCAAGAGCCCAAGTACCGGCGCCCCGACGCCTCCCGCCGCGGACCGCTC
CGCGCTCTGCCCTGAATCGC

Downstream 100 bases:

>100_bases
CGCCCGCCACCCGCCGCAGGCCTTGCCGAACGCGATTCTCCTGCTTGGCCGGATTCGCCCGCGGCGGGCATGAAAAAAAG
GGGTTTCACGTGAAACCCCT

Product: PAS/PAC sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MEIYQRIFEYAPDAMLVLGRDGLLRLVNAHAETLFGYDRSELLGQTVELLVPARFTAQHAAHRARFMEEAQSRQMGRQQD
LYALRKDGSEFPVDIMLSPMIMGGEHLTLCVVRDITERKAAQDALEHQTNELLRLHAELELLANHDGLTGLYNWRAFYEH
AGQLLTTAHRRHEKASLLILDLDHFKQINDRFGHAEGDRVLQAAAAALKASARQNDIVARHGGEEFVIAGFGLSEAESLV
AAERLRAAVASIENLACRVTTSVGVATFVPDPSSRHEPPTLLADLLDRADQALYHAKRSGRNRICHFDQLGEDIAPASDS
P

Sequences:

>Translated_321_residues
MEIYQRIFEYAPDAMLVLGRDGLLRLVNAHAETLFGYDRSELLGQTVELLVPARFTAQHAAHRARFMEEAQSRQMGRQQD
LYALRKDGSEFPVDIMLSPMIMGGEHLTLCVVRDITERKAAQDALEHQTNELLRLHAELELLANHDGLTGLYNWRAFYEH
AGQLLTTAHRRHEKASLLILDLDHFKQINDRFGHAEGDRVLQAAAAALKASARQNDIVARHGGEEFVIAGFGLSEAESLV
AAERLRAAVASIENLACRVTTSVGVATFVPDPSSRHEPPTLLADLLDRADQALYHAKRSGRNRICHFDQLGEDIAPASDS
P
>Mature_321_residues
MEIYQRIFEYAPDAMLVLGRDGLLRLVNAHAETLFGYDRSELLGQTVELLVPARFTAQHAAHRARFMEEAQSRQMGRQQD
LYALRKDGSEFPVDIMLSPMIMGGEHLTLCVVRDITERKAAQDALEHQTNELLRLHAELELLANHDGLTGLYNWRAFYEH
AGQLLTTAHRRHEKASLLILDLDHFKQINDRFGHAEGDRVLQAAAAALKASARQNDIVARHGGEEFVIAGFGLSEAESLV
AAERLRAAVASIENLACRVTTSVGVATFVPDPSSRHEPPTLLADLLDRADQALYHAKRSGRNRICHFDQLGEDIAPASDS
P

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=311, Percent_Identity=35.3697749196142, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=39.4285714285714, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=183, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=37.9518072289157, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788381, Length=291, Percent_Identity=27.8350515463918, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787802, Length=223, Percent_Identity=30.4932735426009, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787541, Length=326, Percent_Identity=25.4601226993865, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787262, Length=186, Percent_Identity=29.0322580645161, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787816, Length=172, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=29.7752808988764, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=31.8471337579618, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=183, Percent_Identity=32.2404371584699, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=83, Percent_Identity=38.5542168674699, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656 [H]

Pfam domain/function: PF00990 GGDEF; PF08448 PAS_4 [H]

EC number: NA

Molecular weight: Translated: 35714; Mature: 35714

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYQRIFEYAPDAMLVLGRDGLLRLVNAHAETLFGYDRSELLGQTVELLVPARFTAQHA
CHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
AHRARFMEEAQSRQMGRQQDLYALRKDGSEFPVDIMLSPMIMGGEHLTLCVVRDITERKA
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHEECCEEECCCEEEEEEHHHHHHHHH
AQDALEHQTNELLRLHAELELLANHDGLTGLYNWRAFYEHAGQLLTTAHRRHEKASLLIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DLDHFKQINDRFGHAEGDRVLQAAAAALKASARQNDIVARHGGEEFVIAGFGLSEAESLV
EHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCHHHHHHHH
AAERLRAAVASIENLACRVTTSVGVATFVPDPSSRHEPPTLLADLLDRADQALYHAKRSG
HHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RNRICHFDQLGEDIAPASDSP
CCCCEEHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEIYQRIFEYAPDAMLVLGRDGLLRLVNAHAETLFGYDRSELLGQTVELLVPARFTAQHA
CHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHH
AHRARFMEEAQSRQMGRQQDLYALRKDGSEFPVDIMLSPMIMGGEHLTLCVVRDITERKA
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHEECCEEECCCEEEEEEHHHHHHHHH
AQDALEHQTNELLRLHAELELLANHDGLTGLYNWRAFYEHAGQLLTTAHRRHEKASLLIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DLDHFKQINDRFGHAEGDRVLQAAAAALKASARQNDIVARHGGEEFVIAGFGLSEAESLV
EHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCHHHHHHHH
AAERLRAAVASIENLACRVTTSVGVATFVPDPSSRHEPPTLLADLLDRADQALYHAKRSG
HHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RNRICHFDQLGEDIAPASDSP
CCCCEEHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]