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The map label for this gene is pcl [H]

Identifier: 74318764

GI number: 74318764

Start: 2825666

End: 2826997

Strand: Direct

Name: pcl [H]

Synonym: Tbd_2746

Alternate gene names: 74318764

Gene position: 2825666-2826997 (Clockwise)

Preceding gene: 74318763

Following gene: 74318765

Centisome position: 97.11

GC content: 73.87

Gene sequence:

>1332_bases
ATGCCGAGCCTGCCGCTTGTCGCCCACCGCCCCGACGCCGTTGTGGCATGGCGCGGCGCCACGCCGGTCACGGCCGCGCA
GTTTGCCGGCGAGGTCGCCGCGCTCGCTGCGGCGCTGCCGCCCGGACGGCACGTCCTCAACCTCTGTCGCGACCGCTACC
GTTTCGCCGTCGCCTTCGCGGCGTCGATCGTGAGCGCGCGCGTCAGCCTGCTGCCGCCGACCCATACGCCAGAGATGGTG
CGCCAGATGCACGCGCGGGCGTCGGACTGCTTCTGCATCGTCGACCACGACGCGGACGTCGGCGCGATGCCGGTCTTCCG
CTACCCCGAACTCGCTGCCGCCCCACCCGCCCAGGTTCCCGAAATCGACGTCACACAGGTCGTCGCCGAGGTCTTTACCT
CGGGGTCGACCGGCACGCCGCTGCCGCACCCGAAACGCTGGGGCCCCCTCGTCGAGAACGTGCGCGCGGAGGCTGCGCGG
CTCGGTGTCGGCCCCGGCCACGCGCTCGTCGGCACGGTCCCGCCGCAGCACATGTACGGGCTCGAGTCGACCGTGCTGAT
GCCGCTGCAGAGCGGGGCCGCCTTCCACGCCGGCCACCCGTTCTATCCGGCCGACGTCGCTGCCGCGCTCGCCGAATTGC
CGCGGCCGCGCGCGCTCGTCACGACGCCTTATCACCTGCGCGCCCTGCTCGCAGCCGACGAGGTACTGCCCGCCGCCGAT
CTGCTCGTCTCCGCGACCGCGCCGCTGTCGGCGACGGTCGCGGCCGAGGCCGAGGCGCGTTTCGGCGCGCCGCTGTTCGA
GATCTACGGCTGCACCGAGACCGGCCAGCTCGCGTCGCGCCGCCCCGCACTCGACGCGGCGTGGCAGACCCTCGGCGCGG
TGCGCCTGCGGCGAGACGGCGAGTCGGCGTGGGCCGAGGGCGGTCACGTCGAACGCCCGACCCGTCTCGGCGACGTCGTC
GAACTCGCGGACGAAGACGGGCGCCGTTTCCTGCTCCACGGCCGCCACGCCGACCTCGTCAACATCGCCGGCAAGCGCAC
GTCGCTCGCCTACCTGAATCACCAGCTTCACGCGATTCCGGGCGTGCGCGACGGCGCCTTCGTGCTGCCGCCGGACGACG
CCGAGGCCAACCCCGAACGCGTACGACGCCTGAGCGCGCTCGTCGTCGCCCCCGACCTCACCCCGGCGGCGCTGATGCAA
GCCTTGCGCGCGCGCATCGACCCGCTCTTTCTGCCGCGCCCGCTCGTCTTCGTCGACGCGCTGCCGCGCAACACCACGGG
CAAGCTCGCGGCCGCCGCCGCACAAGCCCTGGTCGCGGAGGCCGGCGCGTGA

Upstream 100 bases:

>100_bases
CTCAAGCTGCCGAAGGTCGAACGCCACGGCATCCTGGACGGCATCCTCGCTTTCTGGAAAACGCCGCCGGGCACGTCCCC
GCGCTGAGCGCCCCGACGCG

Downstream 100 bases:

>100_bases
GCGAGCACTCACTACCGCTGGCGATCGCGGCCGACCACGCGGCCTACGCCGGGCATTTCCCGGGCCGTCCGATCCTGCCC
GGCGTCGTGCTGCTCGACGA

Product: CoA ligase

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MPSLPLVAHRPDAVVAWRGATPVTAAQFAGEVAALAAALPPGRHVLNLCRDRYRFAVAFAASIVSARVSLLPPTHTPEMV
RQMHARASDCFCIVDHDADVGAMPVFRYPELAAAPPAQVPEIDVTQVVAEVFTSGSTGTPLPHPKRWGPLVENVRAEAAR
LGVGPGHALVGTVPPQHMYGLESTVLMPLQSGAAFHAGHPFYPADVAAALAELPRPRALVTTPYHLRALLAADEVLPAAD
LLVSATAPLSATVAAEAEARFGAPLFEIYGCTETGQLASRRPALDAAWQTLGAVRLRRDGESAWAEGGHVERPTRLGDVV
ELADEDGRRFLLHGRHADLVNIAGKRTSLAYLNHQLHAIPGVRDGAFVLPPDDAEANPERVRRLSALVVAPDLTPAALMQ
ALRARIDPLFLPRPLVFVDALPRNTTGKLAAAAAQALVAEAGA

Sequences:

>Translated_443_residues
MPSLPLVAHRPDAVVAWRGATPVTAAQFAGEVAALAAALPPGRHVLNLCRDRYRFAVAFAASIVSARVSLLPPTHTPEMV
RQMHARASDCFCIVDHDADVGAMPVFRYPELAAAPPAQVPEIDVTQVVAEVFTSGSTGTPLPHPKRWGPLVENVRAEAAR
LGVGPGHALVGTVPPQHMYGLESTVLMPLQSGAAFHAGHPFYPADVAAALAELPRPRALVTTPYHLRALLAADEVLPAAD
LLVSATAPLSATVAAEAEARFGAPLFEIYGCTETGQLASRRPALDAAWQTLGAVRLRRDGESAWAEGGHVERPTRLGDVV
ELADEDGRRFLLHGRHADLVNIAGKRTSLAYLNHQLHAIPGVRDGAFVLPPDDAEANPERVRRLSALVVAPDLTPAALMQ
ALRARIDPLFLPRPLVFVDALPRNTTGKLAAAAAQALVAEAGA
>Mature_442_residues
PSLPLVAHRPDAVVAWRGATPVTAAQFAGEVAALAAALPPGRHVLNLCRDRYRFAVAFAASIVSARVSLLPPTHTPEMVR
QMHARASDCFCIVDHDADVGAMPVFRYPELAAAPPAQVPEIDVTQVVAEVFTSGSTGTPLPHPKRWGPLVENVRAEAARL
GVGPGHALVGTVPPQHMYGLESTVLMPLQSGAAFHAGHPFYPADVAAALAELPRPRALVTTPYHLRALLAADEVLPAADL
LVSATAPLSATVAAEAEARFGAPLFEIYGCTETGQLASRRPALDAAWQTLGAVRLRRDGESAWAEGGHVERPTRLGDVVE
LADEDGRRFLLHGRHADLVNIAGKRTSLAYLNHQLHAIPGVRDGAFVLPPDDAEANPERVRRLSALVVAPDLTPAALMQA
LRARIDPLFLPRPLVFVDALPRNTTGKLAAAAAQALVAEAGA

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 46855; Mature: 46724

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLPLVAHRPDAVVAWRGATPVTAAQFAGEVAALAAALPPGRHVLNLCRDRYRFAVAFA
CCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ASIVSARVSLLPPTHTPEMVRQMHARASDCFCIVDHDADVGAMPVFRYPELAAAPPAQVP
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
EIDVTQVVAEVFTSGSTGTPLPHPKRWGPLVENVRAEAARLGVGPGHALVGTVPPQHMYG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHC
LESTVLMPLQSGAAFHAGHPFYPADVAAALAELPRPRALVTTPYHLRALLAADEVLPAAD
CCCEEEEEECCCCEECCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCHHH
LLVSATAPLSATVAAEAEARFGAPLFEIYGCTETGQLASRRPALDAAWQTLGAVRLRRDG
HHHCCCCCCEEEEEHHHHHHHCCCHHEEECCCCCCHHHHCCCHHHHHHHHHHHHHEECCC
ESAWAEGGHVERPTRLGDVVELADEDGRRFLLHGRHADLVNIAGKRTSLAYLNHQLHAIP
CHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHCCC
GVRDGAFVLPPDDAEANPERVRRLSALVVAPDLTPAALMQALRARIDPLFLPRPLVFVDA
CCCCCEEEECCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
LPRNTTGKLAAAAAQALVAEAGA
CCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSLPLVAHRPDAVVAWRGATPVTAAQFAGEVAALAAALPPGRHVLNLCRDRYRFAVAFA
CCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ASIVSARVSLLPPTHTPEMVRQMHARASDCFCIVDHDADVGAMPVFRYPELAAAPPAQVP
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
EIDVTQVVAEVFTSGSTGTPLPHPKRWGPLVENVRAEAARLGVGPGHALVGTVPPQHMYG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHC
LESTVLMPLQSGAAFHAGHPFYPADVAAALAELPRPRALVTTPYHLRALLAADEVLPAAD
CCCEEEEEECCCCEECCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCHHH
LLVSATAPLSATVAAEAEARFGAPLFEIYGCTETGQLASRRPALDAAWQTLGAVRLRRDG
HHHCCCCCCEEEEEHHHHHHHCCCHHEEECCCCCCHHHHCCCHHHHHHHHHHHHHEECCC
ESAWAEGGHVERPTRLGDVVELADEDGRRFLLHGRHADLVNIAGKRTSLAYLNHQLHAIP
CHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHCCC
GVRDGAFVLPPDDAEANPERVRRLSALVVAPDLTPAALMQALRARIDPLFLPRPLVFVDA
CCCCCEEEECCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
LPRNTTGKLAAAAAQALVAEAGA
CCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA