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The map label for this gene is purK [H]

Identifier: 74318680

GI number: 74318680

Start: 2735850

End: 2737004

Strand: Direct

Name: purK [H]

Synonym: Tbd_2662

Alternate gene names: 74318680

Gene position: 2735850-2737004 (Clockwise)

Preceding gene: 74318679

Following gene: 74318681

Centisome position: 94.02

GC content: 68.83

Gene sequence:

>1155_bases
ATGTCGAATGAGGCGCAGCCGACGATGACTGCCCTGCCGATCCTGCCGGGTGCGACGCTCGGCATCCTGGGAGGCGGTCA
ACTCGGCCGCATGTTCACGATCGCCGCACGCACCATGGGCTACAAGGTCATGGTGCTCGATCCTGACTTCGCGAGTCCGG
CCGGGCAGATGGCCGACGTCCACCTGCAGGCCGACTACACCGACCACGGCGCCCTGCGCCAGCTCGGCGCGGCCTGCGCC
GCGGTGACGACCGAATTCGAGAACGTACCGGCACAAAGCCTGATAGAACTCGCGAACCATTGCCGGGTCTCGCCCGGTGC
CGCCGCAGTCGCGATCGCGCAGGACCGCAGCCACGAGAAATCCTGGCTCGCGCAGAACGGTTTCGCGACGGCGCCCTTCG
CGCTGGTGTACAGCGAAGGCGACCTCGATGCGGCGCTCGCCGAGGTCGGCACCCCCGCGCTGCTCAAGGTCGCGCGCTTC
GGCTACGACGGCAAGGGACAGGCGCGCGTCACCGACCGCGACGGCGCGCGCGCGGCTTTCGCCGAATTCGGTGCCCAGCC
CTGCGTGCTCGAAGGCTTCGTCAAGCTCGAAGGCGAGCTATCGGTCGTCCTCGCGCGCGACGACGCGGGGCATTGCGCGA
CCTTCCCCGTCGCCGAAAACCGCCACGAGCAGGGCATCCTCGACGTCTCGATCGTCCCCGCCCGTGTCGCCGATTCGCTC
GCCGATGCCGCGCGCACGATGGCGCAAGACATCGCAGGAAAACTCGCCTACGTCGGCGTCATGGCGGTCGAGTTCTTCGT
CGTCGACGGTCGCCTGCTCGTCAACGAGATCGCCCCGCGCCCGCACAACTCGGGGCATTACACGCTCGACGCCTGCGTCA
CCGACCAGTTCGAGCAGCAGGTGCGCGCGCTATGCGGCCTGCCGCTCGGCGACACGCGCCTGCTCTCGCCGGTCGCGATG
GTCAACATACTCGGCGACCGCTGGCACGACGGCGGGCCGCACTGGTCGGCGCTGCTCGCGCATCCGGCGATCAAGCTGCA
TCTGTACGGCAAGGAGACCGCGCGCCCCGGCCGCAAGATGGGGCACTTCAACGTGCTCGACACGGACGTCGAGGCAGCGC
TGAAACTCGCCGAAGCGATGCGCGATGCGCTCTAG

Upstream 100 bases:

>100_bases
GCGGCGGCGCTGATCGCGCGCGCCGACCCCGGCCTCGCGGCGCAGCTCAACGAATTCCGCCGCGGCCAGTCCGATTCGGT
GCTCGCGATGGAGCTGCCCG

Downstream 100 bases:

>100_bases
CCGGGTGTTGCGGCAGGCGGGCGGAGTCGCCTTCGCACTACTCCTGGCTGGCGCCGCCGACGCGGGCGGCGATTCGGCAA
CGCTGCGCGTCGGTCCCCAC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSNEAQPTMTALPILPGATLGILGGGQLGRMFTIAARTMGYKVMVLDPDFASPAGQMADVHLQADYTDHGALRQLGAACA
AVTTEFENVPAQSLIELANHCRVSPGAAAVAIAQDRSHEKSWLAQNGFATAPFALVYSEGDLDAALAEVGTPALLKVARF
GYDGKGQARVTDRDGARAAFAEFGAQPCVLEGFVKLEGELSVVLARDDAGHCATFPVAENRHEQGILDVSIVPARVADSL
ADAARTMAQDIAGKLAYVGVMAVEFFVVDGRLLVNEIAPRPHNSGHYTLDACVTDQFEQQVRALCGLPLGDTRLLSPVAM
VNILGDRWHDGGPHWSALLAHPAIKLHLYGKETARPGRKMGHFNVLDTDVEAALKLAEAMRDAL

Sequences:

>Translated_384_residues
MSNEAQPTMTALPILPGATLGILGGGQLGRMFTIAARTMGYKVMVLDPDFASPAGQMADVHLQADYTDHGALRQLGAACA
AVTTEFENVPAQSLIELANHCRVSPGAAAVAIAQDRSHEKSWLAQNGFATAPFALVYSEGDLDAALAEVGTPALLKVARF
GYDGKGQARVTDRDGARAAFAEFGAQPCVLEGFVKLEGELSVVLARDDAGHCATFPVAENRHEQGILDVSIVPARVADSL
ADAARTMAQDIAGKLAYVGVMAVEFFVVDGRLLVNEIAPRPHNSGHYTLDACVTDQFEQQVRALCGLPLGDTRLLSPVAM
VNILGDRWHDGGPHWSALLAHPAIKLHLYGKETARPGRKMGHFNVLDTDVEAALKLAEAMRDAL
>Mature_383_residues
SNEAQPTMTALPILPGATLGILGGGQLGRMFTIAARTMGYKVMVLDPDFASPAGQMADVHLQADYTDHGALRQLGAACAA
VTTEFENVPAQSLIELANHCRVSPGAAAVAIAQDRSHEKSWLAQNGFATAPFALVYSEGDLDAALAEVGTPALLKVARFG
YDGKGQARVTDRDGARAAFAEFGAQPCVLEGFVKLEGELSVVLARDDAGHCATFPVAENRHEQGILDVSIVPARVADSLA
DAARTMAQDIAGKLAYVGVMAVEFFVVDGRLLVNEIAPRPHNSGHYTLDACVTDQFEQQVRALCGLPLGDTRLLSPVAMV
NILGDRWHDGGPHWSALLAHPAIKLHLYGKETARPGRKMGHFNVLDTDVEAALKLAEAMRDAL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=360, Percent_Identity=33.6111111111111, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788155, Length=391, Percent_Identity=26.8542199488491, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324702, Length=364, Percent_Identity=34.6153846153846, Blast_Score=212, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40739; Mature: 40608

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEAQPTMTALPILPGATLGILGGGQLGRMFTIAARTMGYKVMVLDPDFASPAGQMADV
CCCCCCCCEEEECCCCCCEEEEECCCCCCHHEEEEEHHHCEEEEEECCCCCCCCCCEEEE
HLQADYTDHGALRQLGAACAAVTTEFENVPAQSLIELANHCRVSPGAAAVAIAQDRSHEK
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
SWLAQNGFATAPFALVYSEGDLDAALAEVGTPALLKVARFGYDGKGQARVTDRDGARAAF
HHHHCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCHHHH
AEFGAQPCVLEGFVKLEGELSVVLARDDAGHCATFPVAENRHEQGILDVSIVPARVADSL
HHHCCCCCEEEHHEEECCCEEEEEEECCCCCEEECCCCCCCCCCCEEEEEEECHHHHHHH
ADAARTMAQDIAGKLAYVGVMAVEFFVVDGRLLVNEIAPRPHNSGHYTLDACVTDQFEQQ
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHCCCCCCCCCCEEEEHHHHHHHHHH
VRALCGLPLGDTRLLSPVAMVNILGDRWHDGGPHWSALLAHPAIKLHLYGKETARPGRKM
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCCCCC
GHFNVLDTDVEAALKLAEAMRDAL
CCCEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNEAQPTMTALPILPGATLGILGGGQLGRMFTIAARTMGYKVMVLDPDFASPAGQMADV
CCCCCCCEEEECCCCCCEEEEECCCCCCHHEEEEEHHHCEEEEEECCCCCCCCCCEEEE
HLQADYTDHGALRQLGAACAAVTTEFENVPAQSLIELANHCRVSPGAAAVAIAQDRSHEK
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
SWLAQNGFATAPFALVYSEGDLDAALAEVGTPALLKVARFGYDGKGQARVTDRDGARAAF
HHHHCCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCHHHH
AEFGAQPCVLEGFVKLEGELSVVLARDDAGHCATFPVAENRHEQGILDVSIVPARVADSL
HHHCCCCCEEEHHEEECCCEEEEEEECCCCCEEECCCCCCCCCCCEEEEEEECHHHHHHH
ADAARTMAQDIAGKLAYVGVMAVEFFVVDGRLLVNEIAPRPHNSGHYTLDACVTDQFEQQ
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHCCCCCCCCCCEEEEHHHHHHHHHH
VRALCGLPLGDTRLLSPVAMVNILGDRWHDGGPHWSALLAHPAIKLHLYGKETARPGRKM
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCCCCC
GHFNVLDTDVEAALKLAEAMRDAL
CCCEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]