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The map label for this gene is pyrB

Identifier: 74318600

GI number: 74318600

Start: 2656930

End: 2657883

Strand: Direct

Name: pyrB

Synonym: Tbd_2582

Alternate gene names: 74318600

Gene position: 2656930-2657883 (Clockwise)

Preceding gene: 74318599

Following gene: 74318601

Centisome position: 91.31

GC content: 66.04

Gene sequence:

>954_bases
ATGAATCCACAACTGACGCCGGACGGCAGGCTGCGCCACCTGCTGACGCTCGACGGCCTGCCGCGCGCGACCCTGACGCA
CATCCTCGACACCGCCGAATCCTTCATGGACGTCGGCGAGCGCGAGGTGAAGAAAGTGCCGCTGTTGCGCGGCAAGTCGA
TTTTCAACCTGTTCTTCGAGCCGTCGACGCGCACGCGCACGACTTTCGAGATCGCAGCGAAGCGTCTGTCGGCCGACGTC
GTGAACCTGAATATCGCGACCTCGAGCCAGACCAAGGGCGAGGCGATCCTCGACACCGTCGACAACCTCTCGGCGATGCA
CGCCGACATGTTCATCGTGCGCCATTCGCAATCCGGCGCCGCGCATTTCATCGCGCGCCACGTCGCCCCGCACATCTCGG
TCGTCAACGCGGGCGACGGCCGCCACGCCCACCCGACGCAGGGCCTCCTGGACATGTTCACGATCCGGCGTTTCAAGGGC
GAATTCCATAACCTGACCGTCGCCATCGTCGGCGACGTGCTGCATTCGCGCGTCGCACGCTCGCAGATCCACGCACTGAC
CACACTCGGCGTGCCGGAGGTGCGCGTGATCGGGCCCAAGACCCTGCTCCCTACCGGCGTCGAGCAGCTCGGCGTCAAGG
TGTTCCACGACATGGCAGCCGGGCTCGCCGGCTGCGACGTCGTGATCATGCTGCGCCTGCAGAACGAACGCATGAAGGGC
GCGCGGCTGCCGTCGGCGCAGGAATACTTCAAATTCTTCGGGCTGACCCCGGAAAAACTCGCGCTCGCCAAGCCGGACGC
GATCGTCATGCACCCCGGCCCGATGAACCGCGGCGTCGAAATCGCGTCCGAGGTCGCAGACGGCCCGCAGTCGGTGATCC
TGCCGCAGGTAACCTACGGCATCGCGGTACGCATGGCGGTCATGGCGCTGCTCGCCGGGGGAGCCTCGACTTGA

Upstream 100 bases:

>100_bases
CGATCCGCCCGGATTTCGCCGCGCTCACACTCGAGGTCCCGCCTCACCAGAACATCAATCTCTCACGCCTCGACGACGGC
CACCTCACGCTCAGCCTCGC

Downstream 100 bases:

>100_bases
AGCGGGCACTGCCCGCGCCGAACCGCGCGCTGCCGACATCACGCAGCCGCCTTTGACCCAAACGCCCATGAAGATACATA
TCAAGAACGGCCGCGTCATC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNPQLTPDGRLRHLLTLDGLPRATLTHILDTAESFMDVGEREVKKVPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSADV
VNLNIATSSQTKGEAILDTVDNLSAMHADMFIVRHSQSGAAHFIARHVAPHISVVNAGDGRHAHPTQGLLDMFTIRRFKG
EFHNLTVAIVGDVLHSRVARSQIHALTTLGVPEVRVIGPKTLLPTGVEQLGVKVFHDMAAGLAGCDVVIMLRLQNERMKG
ARLPSAQEYFKFFGLTPEKLALAKPDAIVMHPGPMNRGVEIASEVADGPQSVILPQVTYGIAVRMAVMALLAGGAST

Sequences:

>Translated_317_residues
MNPQLTPDGRLRHLLTLDGLPRATLTHILDTAESFMDVGEREVKKVPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSADV
VNLNIATSSQTKGEAILDTVDNLSAMHADMFIVRHSQSGAAHFIARHVAPHISVVNAGDGRHAHPTQGLLDMFTIRRFKG
EFHNLTVAIVGDVLHSRVARSQIHALTTLGVPEVRVIGPKTLLPTGVEQLGVKVFHDMAAGLAGCDVVIMLRLQNERMKG
ARLPSAQEYFKFFGLTPEKLALAKPDAIVMHPGPMNRGVEIASEVADGPQSVILPQVTYGIAVRMAVMALLAGGAST
>Mature_317_residues
MNPQLTPDGRLRHLLTLDGLPRATLTHILDTAESFMDVGEREVKKVPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSADV
VNLNIATSSQTKGEAILDTVDNLSAMHADMFIVRHSQSGAAHFIARHVAPHISVVNAGDGRHAHPTQGLLDMFTIRRFKG
EFHNLTVAIVGDVLHSRVARSQIHALTTLGVPEVRVIGPKTLLPTGVEQLGVKVFHDMAAGLAGCDVVIMLRLQNERMKG
ARLPSAQEYFKFFGLTPEKLALAKPDAIVMHPGPMNRGVEIASEVADGPQSVILPQVTYGIAVRMAVMALLAGGAST

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=35.5769230769231, Blast_Score=168, Evalue=5e-42,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.9805825242718, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=24.9249249249249, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790703, Length=336, Percent_Identity=25.5952380952381, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=31.4024390243902, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=33.1210191082803, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24642586, Length=317, Percent_Identity=35.0157728706625, Blast_Score=158, Evalue=4e-39,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_THIDA (Q3SFS1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316340.1
- HSSP:   P0A786
- ProteinModelPortal:   Q3SFS1
- SMR:   Q3SFS1
- STRING:   Q3SFS1
- GeneID:   3672558
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2582
- NMPDR:   fig|292415.3.peg.2655
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q3SFS1
- ProtClustDB:   PRK00856
- BioCyc:   TDEN292415:TBD_2582-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34349; Mature: 34349

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQLTPDGRLRHLLTLDGLPRATLTHILDTAESFMDVGEREVKKVPLLRGKSIFNLFFE
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEC
PSTRTRTTFEIAAKRLSADVVNLNIATSSQTKGEAILDTVDNLSAMHADMFIVRHSQSGA
CCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCH
AHFIARHVAPHISVVNAGDGRHAHPTQGLLDMFTIRRFKGEFHNLTVAIVGDVLHSRVAR
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHH
SQIHALTTLGVPEVRVIGPKTLLPTGVEQLGVKVFHDMAAGLAGCDVVIMLRLQNERMKG
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
ARLPSAQEYFKFFGLTPEKLALAKPDAIVMHPGPMNRGVEIASEVADGPQSVILPQVTYG
CCCCCHHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCHHHHHHHCCCCCCEECHHHHHH
IAVRMAVMALLAGGAST
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNPQLTPDGRLRHLLTLDGLPRATLTHILDTAESFMDVGEREVKKVPLLRGKSIFNLFFE
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEC
PSTRTRTTFEIAAKRLSADVVNLNIATSSQTKGEAILDTVDNLSAMHADMFIVRHSQSGA
CCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCH
AHFIARHVAPHISVVNAGDGRHAHPTQGLLDMFTIRRFKGEFHNLTVAIVGDVLHSRVAR
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHH
SQIHALTTLGVPEVRVIGPKTLLPTGVEQLGVKVFHDMAAGLAGCDVVIMLRLQNERMKG
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
ARLPSAQEYFKFFGLTPEKLALAKPDAIVMHPGPMNRGVEIASEVADGPQSVILPQVTYG
CCCCCHHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCHHHHHHHCCCCCCEECHHHHHH
IAVRMAVMALLAGGAST
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA