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The map label for this gene is narQ [H]

Identifier: 74318481

GI number: 74318481

Start: 2536185

End: 2537432

Strand: Direct

Name: narQ [H]

Synonym: Tbd_2463

Alternate gene names: 74318481

Gene position: 2536185-2537432 (Clockwise)

Preceding gene: 74318480

Following gene: 74318482

Centisome position: 87.16

GC content: 65.3

Gene sequence:

>1248_bases
ATGCTGAAAGTCTCCGCAGCAGATCGCCTGATGAAAGACAACACCCATCCCCTCGTCCAACACCCCGCGCGCGACGCTGC
CGAGGTCAGCATCCCGGCGCTGCGCGTCCTTTCGGAAATCACGACCAGCCTGTCGACCGACGCCAACGTCGAACAGCTGC
TCGGACGATTCCTCTCGACGATGATCCGCCTTGCCGGCGCTCAGGGCGGGGCCGTGCGCGTGATCACCGACGACCGCACC
CACCTGCGCCTGATCGCCTCGGTCGGCCTGCCACCCGACCTGGTCGAGCACGAGCACCTCGTCAGCGTCGACTGCGGGGT
CTGCGGCAAGGCGGCGCGCGAGGTCTCGGTCAGCAGCTGGAACAACGTCGCGTACTGCAAGCGCCAGGCCAACGATCCCT
ACTTCAGCGGTACGTGCAGCACGGTCGTCGCCGTGCCGCTGATGCACAAGAACCAGGTCATGGGCGTCTACAACCTTTTC
CTCGAAGAAGGCCACGTCGTCCCCGAGGACGTCCGCCTGCTGTTTCGCTCGATCAGCGAGCATCTCGGCATCGCGCTCGA
GAATGCGCGGCTGACCCGCGAGAACATGCGCATTTCGCTCATGAACGAGCGGCAGATGCTGGCCAACCAGATCCACGATT
CGCTCGCGCAGACACTCGCCTACGCCAAGATGCGGCTGACCGTGCTGTCCGACGCGATGCGGCACGAGGACTACGCCAAG
GCCAACCGCTTCCTCGGCGACGTCGAGGAGGCGGTCGACCTCGCCTACGCCGACCTGCGCGACCTCATCACCCAGTACCG
CGATCGCATCAACCCGCGCGGGCTGGTGCCCGCGATCCAGGAGCTCGCCAAGGGCTTCCGCAAGAAGGCCAATGCCGATG
TCGATTTCCTCAACCTGGTGCAGGACGTCACGCTGACGCCCGACGAGGAAATCCAGGTCTTCCACATCATCCAGGAATCG
CTCTACAACATCGCCAAGCACGCGCGGGCGCGCCACGTCGTCATCACCTTCGACCTCGAGAACGGGCAGTACATCGTCAA
CGTCGCCGACGACGGCGTCGGCGTGCAGAAGAAGCACGACGAGTCGTCGACCCTGGGCAAGAGCTTCGGCCTCACGATCA
TGCGCGAACGCGCTGCGCGACTGAACGGCAAGCTCGTCGTCGAGTCGCGACCCGCCGGCGGCACGGTCGTGCGTCTGGTC
TTTCCGCAGCGCGCCGCCTCCCACCAAAACAGCGAGCACGCCGCATGA

Upstream 100 bases:

>100_bases
CGAGGACCTCGAACCGAGCGTCGTCGTCCCCGTCCCGGCCACGCCGGCGGCGCCGACGCCGGCGCCCTGACGGCGTTTGG
CTTCATATGCGAATGGGAAT

Downstream 100 bases:

>100_bases
GCCTGACCCGCATCATCCTCGTCGACGATCACACGCTGTTCCGCAAGGGGCTCGCCGAGCTGCTCGAACAGAGCGGCGAA
ATCGAGGTCACCGCCTTCAC

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLKVSAADRLMKDNTHPLVQHPARDAAEVSIPALRVLSEITTSLSTDANVEQLLGRFLSTMIRLAGAQGGAVRVITDDRT
HLRLIASVGLPPDLVEHEHLVSVDCGVCGKAAREVSVSSWNNVAYCKRQANDPYFSGTCSTVVAVPLMHKNQVMGVYNLF
LEEGHVVPEDVRLLFRSISEHLGIALENARLTRENMRISLMNERQMLANQIHDSLAQTLAYAKMRLTVLSDAMRHEDYAK
ANRFLGDVEEAVDLAYADLRDLITQYRDRINPRGLVPAIQELAKGFRKKANADVDFLNLVQDVTLTPDEEIQVFHIIQES
LYNIAKHARARHVVITFDLENGQYIVNVADDGVGVQKKHDESSTLGKSFGLTIMRERAARLNGKLVVESRPAGGTVVRLV
FPQRAASHQNSEHAA

Sequences:

>Translated_415_residues
MLKVSAADRLMKDNTHPLVQHPARDAAEVSIPALRVLSEITTSLSTDANVEQLLGRFLSTMIRLAGAQGGAVRVITDDRT
HLRLIASVGLPPDLVEHEHLVSVDCGVCGKAAREVSVSSWNNVAYCKRQANDPYFSGTCSTVVAVPLMHKNQVMGVYNLF
LEEGHVVPEDVRLLFRSISEHLGIALENARLTRENMRISLMNERQMLANQIHDSLAQTLAYAKMRLTVLSDAMRHEDYAK
ANRFLGDVEEAVDLAYADLRDLITQYRDRINPRGLVPAIQELAKGFRKKANADVDFLNLVQDVTLTPDEEIQVFHIIQES
LYNIAKHARARHVVITFDLENGQYIVNVADDGVGVQKKHDESSTLGKSFGLTIMRERAARLNGKLVVESRPAGGTVVRLV
FPQRAASHQNSEHAA
>Mature_415_residues
MLKVSAADRLMKDNTHPLVQHPARDAAEVSIPALRVLSEITTSLSTDANVEQLLGRFLSTMIRLAGAQGGAVRVITDDRT
HLRLIASVGLPPDLVEHEHLVSVDCGVCGKAAREVSVSSWNNVAYCKRQANDPYFSGTCSTVVAVPLMHKNQVMGVYNLF
LEEGHVVPEDVRLLFRSISEHLGIALENARLTRENMRISLMNERQMLANQIHDSLAQTLAYAKMRLTVLSDAMRHEDYAK
ANRFLGDVEEAVDLAYADLRDLITQYRDRINPRGLVPAIQELAKGFRKKANADVDFLNLVQDVTLTPDEEIQVFHIIQES
LYNIAKHARARHVVITFDLENGQYIVNVADDGVGVQKKHDESSTLGKSFGLTIMRERAARLNGKLVVESRPAGGTVVRLV
FPQRAASHQNSEHAA

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=269, Percent_Identity=29.368029739777, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787474, Length=253, Percent_Identity=28.8537549407115, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46021; Mature: 46021

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVSAADRLMKDNTHPLVQHPARDAAEVSIPALRVLSEITTSLSTDANVEQLLGRFLST
CEECCHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
MIRLAGAQGGAVRVITDDRTHLRLIASVGLPPDLVEHEHLVSVDCGVCGKAAREVSVSSW
HHHHHCCCCCEEEEEECCCHHEEEEECCCCCHHHHCCCCEEEECCCCCCHHHHHCCCCCC
NNVAYCKRQANDPYFSGTCSTVVAVPLMHKNQVMGVYNLFLEEGHVVPEDVRLLFRSISE
CCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HLGIALENARLTRENMRISLMNERQMLANQIHDSLAQTLAYAKMRLTVLSDAMRHEDYAK
HHCEEECCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANRFLGDVEEAVDLAYADLRDLITQYRDRINPRGLVPAIQELAKGFRKKANADVDFLNLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
QDVTLTPDEEIQVFHIIQESLYNIAKHARARHVVITFDLENGQYIVNVADDGVGVQKKHD
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCC
ESSTLGKSFGLTIMRERAARLNGKLVVESRPAGGTVVRLVFPQRAASHQNSEHAA
CHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCHHHCCCCCCCCC
>Mature Secondary Structure
MLKVSAADRLMKDNTHPLVQHPARDAAEVSIPALRVLSEITTSLSTDANVEQLLGRFLST
CEECCHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
MIRLAGAQGGAVRVITDDRTHLRLIASVGLPPDLVEHEHLVSVDCGVCGKAAREVSVSSW
HHHHHCCCCCEEEEEECCCHHEEEEECCCCCHHHHCCCCEEEECCCCCCHHHHHCCCCCC
NNVAYCKRQANDPYFSGTCSTVVAVPLMHKNQVMGVYNLFLEEGHVVPEDVRLLFRSISE
CCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HLGIALENARLTRENMRISLMNERQMLANQIHDSLAQTLAYAKMRLTVLSDAMRHEDYAK
HHCEEECCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANRFLGDVEEAVDLAYADLRDLITQYRDRINPRGLVPAIQELAKGFRKKANADVDFLNLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
QDVTLTPDEEIQVFHIIQESLYNIAKHARARHVVITFDLENGQYIVNVADDGVGVQKKHD
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCC
ESSTLGKSFGLTIMRERAARLNGKLVVESRPAGGTVVRLVFPQRAASHQNSEHAA
CHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]