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The map label for this gene is uvrD [H]

Identifier: 74318467

GI number: 74318467

Start: 2518254

End: 2520434

Strand: Direct

Name: uvrD [H]

Synonym: Tbd_2449

Alternate gene names: 74318467

Gene position: 2518254-2520434 (Clockwise)

Preceding gene: 74318465

Following gene: 74318471

Centisome position: 86.54

GC content: 67.77

Gene sequence:

>2181_bases
ATGTCCTCCTCCCCCGACGGGCACCAGCGCCTGCTGGATTCGCTCAATCCCGAACAGCGCGCCGCGGTGACGCTGCCGCA
CGAGTCCGCGCTGATCCTCGCCGGGGCCGGCTCGGGCAAGACGCGCGTGCTGACCACGCGCATCGTCTGGCTCGTCCAGA
CCGGCCAGGTGTCGCCGCACGGCATCCTCGCCGTGACCTTCACCAACAAGGCGGCGAAGGAGATGCTCACGCGCATTTCG
GCGATGCTGCCGATCAACACACGCGGCATGTGGGTCGGCACCTTCCACGGCCTCGCCAATCGCCTGTTGCGCACGCACTG
GCGAGAAGCCGGATTGCCGCAGTCCTTCCAGATCCTCGACGCCTCCGACCAGCTCTCGGCGATCAAGCGGCTCATGAAGG
CGATGAACGTCGACACCGAGAAATACGAGCCGAAGAAGGTGCAGTGGTTCATCAACGGCCACAAGGAGGCCGGACGCCGC
GCGCGCGACGCCGAGGCCTACGACGAGTACTCGATGCGGCTGGCCGAGATCTACGAAGCCTACGACGCGCAGTGCCAGCG
CGAGGGCGTGGCCGACTTCCCCGAACTGCTGCTGCGCTCCTACGAATTGCTCTACCGCAACGAGCCGCTCAGGCAGCATT
ACCAGGATCGCTTCCGGCACATCCTGGTCGACGAGTTCCAGGACACCAACACGCTGCAATACCGCTGGCTCAAGCTCTTC
GCCGGCGCGCACAGCGTGATCTTCGCGGTCGGCGACGACGACCAGTCGATCTACGCCTTCCGCGGCGCCGACACCGCCAA
CATGGCCGCGTTCGAACGCGAATTCGCGCACGGCAACGTGATCAAGCTCGAGCAGAACTACCGCAGCCACGGCAACATCC
TGACCGCCGCGAACACGCTGATCGCGCAGAATGCCCACCGCCTCGGCAAGAACCTGTGGACCGCCGCGGGCGAAGGTGAG
CCGATCCGCGTCTTCGAGGCGTATTCCGACCAGGACGAGGCGAGTTTCGTCGTCGACGAGGTGCGGAATCTGAACCGGGA
AGGCATCGCCTACGCCGACATGGCGCTGCTTTACCGCTCCAACGCGCAGTCGCGCGTGCTCGAACACGCCCTGTTCTCGG
CCGGCGTCGCCTATCGCGTATACGGCGGCCTGCGCTTCTTCGAGCGCCAGGAAGTCAAGCACGCCCTCGCCTACCTGCGT
CTTCTGACCCACCCCGAGGACGACGGCGCCTTCCTGCGCGTGGTCAACTTCCCCACCCGCGGCATCGGCGCGCGCACGCT
CGAGCAATTGTCCGAGACCGCCGCGCGCGCGGGAACGAGCCTCTGGCAGGCCGCCTGCACCAGCGGCGGCAAGGTCGCGG
CGTTCGCGCGGCTGATCGAGGACATCAAGCGCGCCACGGCCGACCTGCCCTTGCCCGAAGCGATCGGCCACGTCGTCGAG
GCGTCGGGCCTGGCCGATTACTACCGTGCCGACAAGGACGGCGCCGACCGCCTGGAGAACCTCAACGAACTCGTCAACGC
GGCCGCGCTTTTCGCCGAGGAGTCGGCTGCCGAGGCTTCGGCGTCCGACGAGCCGGCGCCGTCCGAACTGGACCGTTTTC
TCGCCCATGCCGCGCTCGAGGCCGGCGAGCACCAGGCCGAGGCGGGGCGCGACGCGCTGCAGTTGATGACCGTGCACGCG
GCGAAGGGCCTCGAGTTCGAGGCGGTTTTCATCACCGGGCTCGAGGAAAGCCTGTTTCCGCACGAGCAAAGCACGCTCCA
GGGCAGCGGGACACACGCCGACGAGGGGCTCGAGGAAGAGCGGCGGCTGATGTACGTCGCGATCACGCGGGCGCGGCGCA
GGTTGTATCTCTCGCACGCGCAATCGCGCATGCTGCACGGCCAGGTGCGCTACGGCATCGCCTCGCGTTTTCTGTCCGAA
CTGCCCGAGCAGGTGCTACTGCACCTCAATCGCCGGGCGACGCCGGCGCATGTGCGCGAAGCGCCGGCGCCATCGCCGCG
GGCCGCGCGCGCCGGCGAAGACACGGGGTATCGCGTCGGTCAGAGCGTCGCGCACGCCAGGTTCGGCACCGGCATCATCA
TCGACTTCGAAGGACGCGGGCCCGACGCGCGCGTGCAGGTCAAGTTCCGTGATGGAACGAAGTGGCTTGCGCTCGCCTAC
GCCAAGCTGTCGCCGGCCTGA

Upstream 100 bases:

>100_bases
GACGCCATGCCGAGCGTGTCGACGAGGTCGCCGAAAAAGAGGATTTTCACCATGGACCGCCCCGTATAATTCGTAGTCGT
TCGGCTGATTCTACCTCCGT

Downstream 100 bases:

>100_bases
GCCGGCGTCGCGTCATCCGCCCACACCGCGAAGATCGCCTGATACGACGCATAGAGCGAGCCGAACAGCACCGGCATCGC
GACCAGCAGGCCCAGCATCA

Product: ATP-dependent DNA helicase UvrD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSSSPDGHQRLLDSLNPEQRAAVTLPHESALILAGAGSGKTRVLTTRIVWLVQTGQVSPHGILAVTFTNKAAKEMLTRIS
AMLPINTRGMWVGTFHGLANRLLRTHWREAGLPQSFQILDASDQLSAIKRLMKAMNVDTEKYEPKKVQWFINGHKEAGRR
ARDAEAYDEYSMRLAEIYEAYDAQCQREGVADFPELLLRSYELLYRNEPLRQHYQDRFRHILVDEFQDTNTLQYRWLKLF
AGAHSVIFAVGDDDQSIYAFRGADTANMAAFEREFAHGNVIKLEQNYRSHGNILTAANTLIAQNAHRLGKNLWTAAGEGE
PIRVFEAYSDQDEASFVVDEVRNLNREGIAYADMALLYRSNAQSRVLEHALFSAGVAYRVYGGLRFFERQEVKHALAYLR
LLTHPEDDGAFLRVVNFPTRGIGARTLEQLSETAARAGTSLWQAACTSGGKVAAFARLIEDIKRATADLPLPEAIGHVVE
ASGLADYYRADKDGADRLENLNELVNAAALFAEESAAEASASDEPAPSELDRFLAHAALEAGEHQAEAGRDALQLMTVHA
AKGLEFEAVFITGLEESLFPHEQSTLQGSGTHADEGLEEERRLMYVAITRARRRLYLSHAQSRMLHGQVRYGIASRFLSE
LPEQVLLHLNRRATPAHVREAPAPSPRAARAGEDTGYRVGQSVAHARFGTGIIIDFEGRGPDARVQVKFRDGTKWLALAY
AKLSPA

Sequences:

>Translated_726_residues
MSSSPDGHQRLLDSLNPEQRAAVTLPHESALILAGAGSGKTRVLTTRIVWLVQTGQVSPHGILAVTFTNKAAKEMLTRIS
AMLPINTRGMWVGTFHGLANRLLRTHWREAGLPQSFQILDASDQLSAIKRLMKAMNVDTEKYEPKKVQWFINGHKEAGRR
ARDAEAYDEYSMRLAEIYEAYDAQCQREGVADFPELLLRSYELLYRNEPLRQHYQDRFRHILVDEFQDTNTLQYRWLKLF
AGAHSVIFAVGDDDQSIYAFRGADTANMAAFEREFAHGNVIKLEQNYRSHGNILTAANTLIAQNAHRLGKNLWTAAGEGE
PIRVFEAYSDQDEASFVVDEVRNLNREGIAYADMALLYRSNAQSRVLEHALFSAGVAYRVYGGLRFFERQEVKHALAYLR
LLTHPEDDGAFLRVVNFPTRGIGARTLEQLSETAARAGTSLWQAACTSGGKVAAFARLIEDIKRATADLPLPEAIGHVVE
ASGLADYYRADKDGADRLENLNELVNAAALFAEESAAEASASDEPAPSELDRFLAHAALEAGEHQAEAGRDALQLMTVHA
AKGLEFEAVFITGLEESLFPHEQSTLQGSGTHADEGLEEERRLMYVAITRARRRLYLSHAQSRMLHGQVRYGIASRFLSE
LPEQVLLHLNRRATPAHVREAPAPSPRAARAGEDTGYRVGQSVAHARFGTGIIIDFEGRGPDARVQVKFRDGTKWLALAY
AKLSPA
>Mature_725_residues
SSSPDGHQRLLDSLNPEQRAAVTLPHESALILAGAGSGKTRVLTTRIVWLVQTGQVSPHGILAVTFTNKAAKEMLTRISA
MLPINTRGMWVGTFHGLANRLLRTHWREAGLPQSFQILDASDQLSAIKRLMKAMNVDTEKYEPKKVQWFINGHKEAGRRA
RDAEAYDEYSMRLAEIYEAYDAQCQREGVADFPELLLRSYELLYRNEPLRQHYQDRFRHILVDEFQDTNTLQYRWLKLFA
GAHSVIFAVGDDDQSIYAFRGADTANMAAFEREFAHGNVIKLEQNYRSHGNILTAANTLIAQNAHRLGKNLWTAAGEGEP
IRVFEAYSDQDEASFVVDEVRNLNREGIAYADMALLYRSNAQSRVLEHALFSAGVAYRVYGGLRFFERQEVKHALAYLRL
LTHPEDDGAFLRVVNFPTRGIGARTLEQLSETAARAGTSLWQAACTSGGKVAAFARLIEDIKRATADLPLPEAIGHVVEA
SGLADYYRADKDGADRLENLNELVNAAALFAEESAAEASASDEPAPSELDRFLAHAALEAGEHQAEAGRDALQLMTVHAA
KGLEFEAVFITGLEESLFPHEQSTLQGSGTHADEGLEEERRLMYVAITRARRRLYLSHAQSRMLHGQVRYGIASRFLSEL
PEQVLLHLNRRATPAHVREAPAPSPRAARAGEDTGYRVGQSVAHARFGTGIIIDFEGRGPDARVQVKFRDGTKWLALAYA
KLSPA

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=732, Percent_Identity=49.0437158469945, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=653, Percent_Identity=37.6722817764165, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=29.5321637426901, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=760, Percent_Identity=29.0789473684211, Blast_Score=217, Evalue=6e-57,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80715; Mature: 80584

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSPDGHQRLLDSLNPEQRAAVTLPHESALILAGAGSGKTRVLTTRIVWLVQTGQVSPH
CCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCCC
GILAVTFTNKAAKEMLTRISAMLPINTRGMWVGTFHGLANRLLRTHWREAGLPQSFQILD
CEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEC
ASDQLSAIKRLMKAMNVDTEKYEPKKVQWFINGHKEAGRRARDAEAYDEYSMRLAEIYEA
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHCCHHCCHHHHHHHHHHHHHHHHH
YDAQCQREGVADFPELLLRSYELLYRNEPLRQHYQDRFRHILVDEFQDTNTLQYRWLKLF
HHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AGAHSVIFAVGDDDQSIYAFRGADTANMAAFEREFAHGNVIKLEQNYRSHGNILTAANTL
HCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHH
IAQNAHRLGKNLWTAAGEGEPIRVFEAYSDQDEASFVVDEVRNLNREGIAYADMALLYRS
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NAQSRVLEHALFSAGVAYRVYGGLRFFERQEVKHALAYLRLLTHPEDDGAFLRVVNFPTR
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GIGARTLEQLSETAARAGTSLWQAACTSGGKVAAFARLIEDIKRATADLPLPEAIGHVVE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ASGLADYYRADKDGADRLENLNELVNAAALFAEESAAEASASDEPAPSELDRFLAHAALE
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AGEHQAEAGRDALQLMTVHAAKGLEFEAVFITGLEESLFPHEQSTLQGSGTHADEGLEEE
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHCCCCHHCCCCCCCCCCCCHHHH
RRLMYVAITRARRRLYLSHAQSRMLHGQVRYGIASRFLSELPEQVLLHLNRRATPAHVRE
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
APAPSPRAARAGEDTGYRVGQSVAHARFGTGIIIDFEGRGPDARVQVKFRDGTKWLALAY
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEE
AKLSPA
HHCCCC
>Mature Secondary Structure 
SSSPDGHQRLLDSLNPEQRAAVTLPHESALILAGAGSGKTRVLTTRIVWLVQTGQVSPH
CCCCCHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCCC
GILAVTFTNKAAKEMLTRISAMLPINTRGMWVGTFHGLANRLLRTHWREAGLPQSFQILD
CEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEC
ASDQLSAIKRLMKAMNVDTEKYEPKKVQWFINGHKEAGRRARDAEAYDEYSMRLAEIYEA
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHCCHHCCHHHHHHHHHHHHHHHHH
YDAQCQREGVADFPELLLRSYELLYRNEPLRQHYQDRFRHILVDEFQDTNTLQYRWLKLF
HHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AGAHSVIFAVGDDDQSIYAFRGADTANMAAFEREFAHGNVIKLEQNYRSHGNILTAANTL
HCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEHHHHH
IAQNAHRLGKNLWTAAGEGEPIRVFEAYSDQDEASFVVDEVRNLNREGIAYADMALLYRS
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NAQSRVLEHALFSAGVAYRVYGGLRFFERQEVKHALAYLRLLTHPEDDGAFLRVVNFPTR
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GIGARTLEQLSETAARAGTSLWQAACTSGGKVAAFARLIEDIKRATADLPLPEAIGHVVE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ASGLADYYRADKDGADRLENLNELVNAAALFAEESAAEASASDEPAPSELDRFLAHAALE
HCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AGEHQAEAGRDALQLMTVHAAKGLEFEAVFITGLEESLFPHEQSTLQGSGTHADEGLEEE
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHCCCCHHCCCCCCCCCCCCHHHH
RRLMYVAITRARRRLYLSHAQSRMLHGQVRYGIASRFLSELPEQVLLHLNRRATPAHVRE
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
APAPSPRAARAGEDTGYRVGQSVAHARFGTGIIIDFEGRGPDARVQVKFRDGTKWLALAY
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEE
AKLSPA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]