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The map label for this gene is rlmH [H]

Identifier: 74318457

GI number: 74318457

Start: 2509437

End: 2509958

Strand: Reverse

Name: rlmH [H]

Synonym: Tbd_2439

Alternate gene names: 74318457

Gene position: 2509958-2509437 (Counterclockwise)

Preceding gene: 74318458

Following gene: 74318456

Centisome position: 86.26

GC content: 70.88

Gene sequence:

>522_bases
GTGGGGGGCCGGCGAAGCGGCGCGCGTACGCAAGGCGACACGCTAGGCCGCATGAAGCTGCAGCTCATCGCAGTCGGGCA
CAAGATGCCGGACTGGATCAACGCGGGGTATGCGGACTATGTGCGGCGCATGCCGGGCGATCTGCAGCTCGCGTTGACCG
AGATCAAGCCGGGGCATCGGGTGGCGGGCGCGGACGGCGCGCGGGCCCGCCAGCTCGAGGCGGCGCGCATCCTCGCCGCT
ATTACGCCCGGCGCGGTGCGCGTCGTGCTCGACGAACGCGGCACGCAGGCGACGACGCGCGAGCTCGCGGGCTGGCTCGA
GAACTGGATGGGCGAGGGGCTCACGCCCGCGTTCGTGATCGGCGGTGCCGACGGGCTCGACGACAGCGTCAAGGCCGGCG
CCGGCAAGCTCTTCGGCCTGTCGCGGCTTACACTGCCGCATGCGCTCGCGCGCGTGATCCTCGCCGAGCAGCTGTACCGT
GCCGCATGCATCATCAAGGGGCATCCCTACCATCGGGATTGA

Upstream 100 bases:

>100_bases
AAGGCGAGGAAAGCGGCGACTGGGTCCTGCTCGACCTCGGCGAAGTGATCGTCCACGTCATGCTGCCGGCCGTGCGCGCG
CATTACAATCTCGAAGAACT

Downstream 100 bases:

>100_bases
GGGGGAATAACGAAAAAATGCTGGTCGACAAACGCATCTACCTGGCTTCGCAATCGCCGCGACGCCGCGAACTTCTGAAA
CAGATCGGCATCGCCTACGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MGGRRSGARTQGDTLGRMKLQLIAVGHKMPDWINAGYADYVRRMPGDLQLALTEIKPGHRVAGADGARARQLEAARILAA
ITPGAVRVVLDERGTQATTRELAGWLENWMGEGLTPAFVIGGADGLDDSVKAGAGKLFGLSRLTLPHALARVILAEQLYR
AACIIKGHPYHRD

Sequences:

>Translated_173_residues
MGGRRSGARTQGDTLGRMKLQLIAVGHKMPDWINAGYADYVRRMPGDLQLALTEIKPGHRVAGADGARARQLEAARILAA
ITPGAVRVVLDERGTQATTRELAGWLENWMGEGLTPAFVIGGADGLDDSVKAGAGKLFGLSRLTLPHALARVILAEQLYR
AACIIKGHPYHRD
>Mature_172_residues
GGRRSGARTQGDTLGRMKLQLIAVGHKMPDWINAGYADYVRRMPGDLQLALTEIKPGHRVAGADGARARQLEAARILAAI
TPGAVRVVLDERGTQATTRELAGWLENWMGEGLTPAFVIGGADGLDDSVKAGAGKLFGLSRLTLPHALARVILAEQLYRA
ACIIKGHPYHRD

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=157, Percent_Identity=47.1337579617834, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 18559; Mature: 18428

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGRRSGARTQGDTLGRMKLQLIAVGHKMPDWINAGYADYVRRMPGDLQLALTEIKPGHR
CCCCCCCCCCCCCCHHHHEEEEEEECCCCCHHHCCCHHHHHHHCCCCCEEEEEECCCCCE
VAGADGARARQLEAARILAAITPGAVRVVLDERGTQATTRELAGWLENWMGEGLTPAFVI
ECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEE
GGADGLDDSVKAGAGKLFGLSRLTLPHALARVILAEQLYRAACIIKGHPYHRD
ECCCCCCCHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure 
GGRRSGARTQGDTLGRMKLQLIAVGHKMPDWINAGYADYVRRMPGDLQLALTEIKPGHR
CCCCCCCCCCCCCHHHHEEEEEEECCCCCHHHCCCHHHHHHHCCCCCEEEEEECCCCCE
VAGADGARARQLEAARILAAITPGAVRVVLDERGTQATTRELAGWLENWMGEGLTPAFVI
ECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEE
GGADGLDDSVKAGAGKLFGLSRLTLPHALARVILAEQLYRAACIIKGHPYHRD
ECCCCCCCHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA