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The map label for this gene is yfjP [H]

Identifier: 74318454

GI number: 74318454

Start: 2506609

End: 2507235

Strand: Reverse

Name: yfjP [H]

Synonym: Tbd_2436

Alternate gene names: 74318454

Gene position: 2507235-2506609 (Counterclockwise)

Preceding gene: 74318455

Following gene: 74318450

Centisome position: 86.16

GC content: 68.42

Gene sequence:

>627_bases
ATGACCGCCCGTCCCCCCGACTACTGGCACGCCGCCTGCAGTGAACTCACCGCGGCCGACCCCGTCATGGCCGGGCTCAT
CGCGCGCTATCCCGACTGCGTGCTCGGCAACCGCGGCGACCCGTTCCAGACCCTCGCGCGCGCAATCGTCGGCCAGCAGA
TCTCGGTCAAGGCCGCCGACAGCATCTGGGCCCGTTTCGCTGCGCTGATGGAAGCGGTGGCGCCTGCCCGGCTCGTCGCA
CTCGATCGCGACACGCTCGCGACCTGCGGGCTGTCGCGACGCAAGGTCGAATATCTCGTCGACCTCGCCGGGCATTTCAT
CGACGGCCGCGTCGAGCCTTCGCGCTGGAAGCGCATGGACGACGAGGACGTCATCGCCGAGCTCGTCGACGTGCGCGGCA
TCGGCCGCTGGACCGCGGAGATGTTCCTGATCTTCAATCTGCAGCGCCCCGACGTCTGGCCGGTCGACGACATCGGTCTG
CAGAAGGCCGTCGCGCTGCATTATCTCGAAGGCGTTCGGCCGACGCCGAAGGTCTTGCGCGCGCACGGCGAACGCCACGC
GCCGTGGCGGACGGTCGCGACCTGGTATTTATGGCGCAGCCTGGACCCTGCGGTCGTGCAGTATTAG

Upstream 100 bases:

>100_bases
TCAGGGGTCAGGGGTCAGGGGTCAGGGGTCAGGGGTCAGGGGTCAGGGGTCAGGGGGCGCGATTGTTCCATCTTGTAATG
AACTCCATCCCGGCGATGCC

Downstream 100 bases:

>100_bases
ACGCCGATCTCCGCCAGCACCGGCGCGTGGTCCGACGGCCGCTCGAGTTTGCGCATGTCGCGGTCGATCGTGCTCGAGGT
GCAGCTCGCGGCGAGCGCGT

Product: 3-methyladenine DNA glycosylase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTARPPDYWHAACSELTAADPVMAGLIARYPDCVLGNRGDPFQTLARAIVGQQISVKAADSIWARFAALMEAVAPARLVA
LDRDTLATCGLSRRKVEYLVDLAGHFIDGRVEPSRWKRMDDEDVIAELVDVRGIGRWTAEMFLIFNLQRPDVWPVDDIGL
QKAVALHYLEGVRPTPKVLRAHGERHAPWRTVATWYLWRSLDPAVVQY

Sequences:

>Translated_208_residues
MTARPPDYWHAACSELTAADPVMAGLIARYPDCVLGNRGDPFQTLARAIVGQQISVKAADSIWARFAALMEAVAPARLVA
LDRDTLATCGLSRRKVEYLVDLAGHFIDGRVEPSRWKRMDDEDVIAELVDVRGIGRWTAEMFLIFNLQRPDVWPVDDIGL
QKAVALHYLEGVRPTPKVLRAHGERHAPWRTVATWYLWRSLDPAVVQY
>Mature_207_residues
TARPPDYWHAACSELTAADPVMAGLIARYPDCVLGNRGDPFQTLARAIVGQQISVKAADSIWARFAALMEAVAPARLVAL
DRDTLATCGLSRRKVEYLVDLAGHFIDGRVEPSRWKRMDDEDVIAELVDVRGIGRWTAEMFLIFNLQRPDVWPVDDIGLQ
KAVALHYLEGVRPTPKVLRAHGERHAPWRTVATWYLWRSLDPAVVQY

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=206, Percent_Identity=31.0679611650485, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320990, Length=240, Percent_Identity=27.9166666666667, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 23388; Mature: 23257

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARPPDYWHAACSELTAADPVMAGLIARYPDCVLGNRGDPFQTLARAIVGQQISVKAAD
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
SIWARFAALMEAVAPARLVALDRDTLATCGLSRRKVEYLVDLAGHFIDGRVEPSRWKRMD
HHHHHHHHHHHHHCCHHHEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCC
DEDVIAELVDVRGIGRWTAEMFLIFNLQRPDVWPVDDIGLQKAVALHYLEGVRPTPKVLR
HHHHHHHHHHHHCCCHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AHGERHAPWRTVATWYLWRSLDPAVVQY
HCCCCCCCHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
TARPPDYWHAACSELTAADPVMAGLIARYPDCVLGNRGDPFQTLARAIVGQQISVKAAD
CCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
SIWARFAALMEAVAPARLVALDRDTLATCGLSRRKVEYLVDLAGHFIDGRVEPSRWKRMD
HHHHHHHHHHHHHCCHHHEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCC
DEDVIAELVDVRGIGRWTAEMFLIFNLQRPDVWPVDDIGLQKAVALHYLEGVRPTPKVLR
HHHHHHHHHHHHCCCHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AHGERHAPWRTVATWYLWRSLDPAVVQY
HCCCCCCCHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]