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The map label for this gene is trmL [H]

Identifier: 74318425

GI number: 74318425

Start: 2479111

End: 2479575

Strand: Reverse

Name: trmL [H]

Synonym: Tbd_2407

Alternate gene names: 74318425

Gene position: 2479575-2479111 (Counterclockwise)

Preceding gene: 74318433

Following gene: 74318424

Centisome position: 85.21

GC content: 67.31

Gene sequence:

>465_bases
ATGTTTGACATTGTTTTATATCAGCCCGAAATCCCGCCCAATACCGGCAATCTGATCCGCCTCGCCGCCAACACCGGCGC
GCGCCTGCACCTCGTCGAGCCGCTCGGCTTCGACCTGTCGGACAAGCAGCTTGTACGCGCCGGGCTCGACTATCACGACA
TGGCAAGCGTCCACGTGCATGCCGACTGGACCGCGGTGCAGGAGGCCCTCGCCGGCCGGCGCTGGTTCGCGCTCACGACC
CGGGGCAGTCGCAGCTATGCGAGCGTGGCGTTCGAGGCCGGCGACGTGCTGCTGTTCGGCCCCGAGTCCCGCGGCCTGCC
GGCCGACCTGCTCGCGCATTTCGACGTCGACCAACGGCTGCGTCTGCCGATGCTGCCGGGTTCGCGCAGCCTCAATCTTT
CCAACGCGGTCAGCGTCGTGGTGTTCGAGGCTTGGCGGCAGAACGGGTTTGCCGGCGGGGGGTGA

Upstream 100 bases:

>100_bases
CGTTTTTCGAGTTCCAGCAAGGGCCCGGTGAGGGCGGTCGTGAGGTAAGGAACCATAGAATAGGCGGACTCCGAGAATGT
TTTATTTTAGGGCAAGCCCG

Downstream 100 bases:

>100_bases
CGGCCGGCGACACAGAACCGACGCATCTCCGCGGCCGCAGACCGCGGCGGCCTAAAGTTTTCTCGGACAATGACGATATA
CATATCACGATATGCCGAAT

Product: RNA methyltransferase CspR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MFDIVLYQPEIPPNTGNLIRLAANTGARLHLVEPLGFDLSDKQLVRAGLDYHDMASVHVHADWTAVQEALAGRRWFALTT
RGSRSYASVAFEAGDVLLFGPESRGLPADLLAHFDVDQRLRLPMLPGSRSLNLSNAVSVVVFEAWRQNGFAGGG

Sequences:

>Translated_154_residues
MFDIVLYQPEIPPNTGNLIRLAANTGARLHLVEPLGFDLSDKQLVRAGLDYHDMASVHVHADWTAVQEALAGRRWFALTT
RGSRSYASVAFEAGDVLLFGPESRGLPADLLAHFDVDQRLRLPMLPGSRSLNLSNAVSVVVFEAWRQNGFAGGG
>Mature_154_residues
MFDIVLYQPEIPPNTGNLIRLAANTGARLHLVEPLGFDLSDKQLVRAGLDYHDMASVHVHADWTAVQEALAGRRWFALTT
RGSRSYASVAFEAGDVLLFGPESRGLPADLLAHFDVDQRLRLPMLPGSRSLNLSNAVSVVVFEAWRQNGFAGGG

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=58.8235294117647, Blast_Score=197, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16812; Mature: 16812

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDIVLYQPEIPPNTGNLIRLAANTGARLHLVEPLGFDLSDKQLVRAGLDYHDMASVHVH
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEE
ADWTAVQEALAGRRWFALTTRGSRSYASVAFEAGDVLLFGPESRGLPADLLAHFDVDQRL
CCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHCCCCCCE
RLPMLPGSRSLNLSNAVSVVVFEAWRQNGFAGGG
ECCCCCCCCCCCCCHHEEEEEEEEHHHCCCCCCC
>Mature Secondary Structure
MFDIVLYQPEIPPNTGNLIRLAANTGARLHLVEPLGFDLSDKQLVRAGLDYHDMASVHVH
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEE
ADWTAVQEALAGRRWFALTTRGSRSYASVAFEAGDVLLFGPESRGLPADLLAHFDVDQRL
CCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHCCCCCCE
RLPMLPGSRSLNLSNAVSVVVFEAWRQNGFAGGG
ECCCCCCCCCCCCCHHEEEEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA