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The map label for this gene is ogt [H]

Identifier: 74318395

GI number: 74318395

Start: 2451507

End: 2451977

Strand: Direct

Name: ogt [H]

Synonym: Tbd_2377

Alternate gene names: 74318395

Gene position: 2451507-2451977 (Clockwise)

Preceding gene: 74318394

Following gene: 74318396

Centisome position: 84.25

GC content: 69.21

Gene sequence:

>471_bases
ATGCCCGCCTACGACGCCGTCCTGCCAGCGCCCATGTGTCGCCTCGGCGCCCGCTTCACCGGGGACGCCCTGACGCGGCT
GGATTTTTTGCCTGCGGACGCCGAAGTCTCGTCCGGCATCGACTCGCGCGCCCGAGAGTTGAGTGTCCAGCTCGAGGCGT
ATTGGCAGGACCCCGGCCATGTCTTCGATCTTTTCTACGTCCCCCTCGGAACGCCGTTTCAACTGCGCGTCTGGCACGCC
CTCATGCGCATCCCGGCGGGCCGGCCGACGACCTACGGGAGCCTTGCCGCCCGCCTGGGAACCGCGCCACGCGCGGTCGG
CCAGGCCTGCGGCGCCAACCCGCTGCCGATCCTGATTCCCTGCCATCGCGTCGTTGCCGCGCGCGGCGTCGGCGGATTCA
TGCACGCCGACTCGGGAATGCCGCTGGACGTGAAGCTCTCGCTGCTGGCCCATGAACGACGCGCTGATTGA

Upstream 100 bases:

>100_bases
TGCCGCCCTGCAAGAACGCCCGCCTCGCGCGGGCGTTTTTTTTGACCCGAGCCCCCACCCTTCCTCTGCACACGGACAAA
GCGCGGGCTAGAATCCTGGA

Downstream 100 bases:

>100_bases
TCGTTTCTGCGACCACCTGTGGCTGGAGGACGGGCTCGCCGAACTGACCCTGGCTGCGTACCGGCGCGACCTCAGAAGCT
TTGCCGGGTGGCTGGACAAA

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MPAYDAVLPAPMCRLGARFTGDALTRLDFLPADAEVSSGIDSRARELSVQLEAYWQDPGHVFDLFYVPLGTPFQLRVWHA
LMRIPAGRPTTYGSLAARLGTAPRAVGQACGANPLPILIPCHRVVAARGVGGFMHADSGMPLDVKLSLLAHERRAD

Sequences:

>Translated_156_residues
MPAYDAVLPAPMCRLGARFTGDALTRLDFLPADAEVSSGIDSRARELSVQLEAYWQDPGHVFDLFYVPLGTPFQLRVWHA
LMRIPAGRPTTYGSLAARLGTAPRAVGQACGANPLPILIPCHRVVAARGVGGFMHADSGMPLDVKLSLLAHERRAD
>Mature_155_residues
PAYDAVLPAPMCRLGARFTGDALTRLDFLPADAEVSSGIDSRARELSVQLEAYWQDPGHVFDLFYVPLGTPFQLRVWHAL
MRIPAGRPTTYGSLAARLGTAPRAVGQACGANPLPILIPCHRVVAARGVGGFMHADSGMPLDVKLSLLAHERRAD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=85, Percent_Identity=48.2352941176471, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788542, Length=63, Percent_Identity=52.3809523809524, Blast_Score=65, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=72, Percent_Identity=45.8333333333333, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16818; Mature: 16686

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYDAVLPAPMCRLGARFTGDALTRLDFLPADAEVSSGIDSRARELSVQLEAYWQDPGH
CCCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCCHHHCCHHHHHHHHEEEEEEEECCCCC
VFDLFYVPLGTPFQLRVWHALMRIPAGRPTTYGSLAARLGTAPRAVGQACGANPLPILIP
EEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCEEEEE
CHRVVAARGVGGFMHADSGMPLDVKLSLLAHERRAD
HHHHHHHCCCCCEEECCCCCCEEEEHHEEHHHCCCC
>Mature Secondary Structure 
PAYDAVLPAPMCRLGARFTGDALTRLDFLPADAEVSSGIDSRARELSVQLEAYWQDPGH
CCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCCHHHCCHHHHHHHHEEEEEEEECCCCC
VFDLFYVPLGTPFQLRVWHALMRIPAGRPTTYGSLAARLGTAPRAVGQACGANPLPILIP
EEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCEEEEE
CHRVVAARGVGGFMHADSGMPLDVKLSLLAHERRAD
HHHHHHHCCCCCEEECCCCCCEEEEHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]