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The map label for this gene is spsI [H]

Identifier: 74318353

GI number: 74318353

Start: 2409044

End: 2409757

Strand: Reverse

Name: spsI [H]

Synonym: Tbd_2335

Alternate gene names: 74318353

Gene position: 2409757-2409044 (Counterclockwise)

Preceding gene: 74318354

Following gene: 74318352

Centisome position: 82.81

GC content: 68.91

Gene sequence:

>714_bases
TTGAGCGTTGCGCCCTCGGCGATGATCCTTGCGGCGGGTCGCGGCGAACGCATGCGTCCCCTGACCGACCACACGCCCAA
GCCGCTTCTGCACGTGGGCGGAAAACCGCTGATCGTCTGGCACATCGAACGGCTGCGCGCCGCCGGCTTCCGCCACGTCG
TCATCAACCACGCGCATCTCGGCGAACAGATTGAGGCGGCGCTCGGCGATGGCGCGGCCTTGGGCGTTGCAATCGATTAT
TCGCGCGAAGCGAGCGCGCTCGAAACGGCGGGCGGCATCGCGACCGCGTTGCCCTTGATCCGTGGCGAGGCCTTTGCCGT
CGTCAACGGCGACATCTATACCGACTACGACTTCGGGCGCCTGGCCGAGCCGCTCACCTGGCTCGCCGCAGGCGGCGACC
AGGCCCATCTCGTCATGGTCGACAACCCGCCGCAGCACCCCAACGGCGATTTCGTGCTCGAAGACGGCCGGGTGAAAAGC
GGCGAGCAGGCCGCCGAGTCATCTCGCCTGACCTTTTCCGGCATCGGCGTCTACCGGCGCGCGCTTTTCGCCGACCTACG
CGCGGGCGAGAAGGCGCCGCTCGCGCCGCTGCTGCGGCAGGCGATGGACGCGGCCCGGGTCAGCGGCGAACATTACAGCG
GGCGCTGGACCGACGTCGGCACGCCGGCGCGTCTTGCCGCGCTCGACGAGGAACTGAGACGACATGCAGCCTGA

Upstream 100 bases:

>100_bases
ATTACCTGCGCAGCGTGTGCGGGCGCTACGACGAACTCAAGCCCTTGCTGCTGCTGCTCGACGCGCTGCAGGACAGGCAG
CCGCAGGCGGGGTACACGTT

Downstream 100 bases:

>100_bases
TTCCGCGATTCATCGCGCGCGTCGCGAACGCGTGCTCGAACAGATGGGCGAGGGCGTGATGGTCATCGCGACCGCGCCCG
AGGTCCCGCGCAACCGCGAC

Product: putative nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSVAPSAMILAAGRGERMRPLTDHTPKPLLHVGGKPLIVWHIERLRAAGFRHVVINHAHLGEQIEAALGDGAALGVAIDY
SREASALETAGGIATALPLIRGEAFAVVNGDIYTDYDFGRLAEPLTWLAAGGDQAHLVMVDNPPQHPNGDFVLEDGRVKS
GEQAAESSRLTFSGIGVYRRALFADLRAGEKAPLAPLLRQAMDAARVSGEHYSGRWTDVGTPARLAALDEELRRHAA

Sequences:

>Translated_237_residues
MSVAPSAMILAAGRGERMRPLTDHTPKPLLHVGGKPLIVWHIERLRAAGFRHVVINHAHLGEQIEAALGDGAALGVAIDY
SREASALETAGGIATALPLIRGEAFAVVNGDIYTDYDFGRLAEPLTWLAAGGDQAHLVMVDNPPQHPNGDFVLEDGRVKS
GEQAAESSRLTFSGIGVYRRALFADLRAGEKAPLAPLLRQAMDAARVSGEHYSGRWTDVGTPARLAALDEELRRHAA
>Mature_236_residues
SVAPSAMILAAGRGERMRPLTDHTPKPLLHVGGKPLIVWHIERLRAAGFRHVVINHAHLGEQIEAALGDGAALGVAIDYS
REASALETAGGIATALPLIRGEAFAVVNGDIYTDYDFGRLAEPLTWLAAGGDQAHLVMVDNPPQHPNGDFVLEDGRVKSG
EQAAESSRLTFSGIGVYRRALFADLRAGEKAPLAPLLRQAMDAARVSGEHYSGRWTDVGTPARLAALDEELRRHAA

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=121, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI11761619, Length=121, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133931050, Length=225, Percent_Identity=29.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=144, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21355443, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644084, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653912, Length=127, Percent_Identity=37.7952755905512, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 25316; Mature: 25185

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAPSAMILAAGRGERMRPLTDHTPKPLLHVGGKPLIVWHIERLRAAGFRHVVINHAHL
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCEEEEEEHHHHHHCCCEEEEEECHHH
GEQIEAALGDGAALGVAIDYSREASALETAGGIATALPLIRGEAFAVVNGDIYTDYDFGR
HHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCHHHHHHHHCCCEEEEEECCEEECCCHHH
LAEPLTWLAAGGDQAHLVMVDNPPQHPNGDFVLEDGRVKSGEQAAESSRLTFSGIGVYRR
HHHHHHEEECCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHCCCEEEEHHHHHHH
ALFADLRAGEKAPLAPLLRQAMDAARVSGEHYSGRWTDVGTPARLAALDEELRRHAA
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVAPSAMILAAGRGERMRPLTDHTPKPLLHVGGKPLIVWHIERLRAAGFRHVVINHAHL
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHCCCCEEEEEEHHHHHHCCCEEEEEECHHH
GEQIEAALGDGAALGVAIDYSREASALETAGGIATALPLIRGEAFAVVNGDIYTDYDFGR
HHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCHHHHHHHHCCCEEEEEECCEEECCCHHH
LAEPLTWLAAGGDQAHLVMVDNPPQHPNGDFVLEDGRVKSGEQAAESSRLTFSGIGVYRR
HHHHHHEEECCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHCCCEEEEHHHHHHH
ALFADLRAGEKAPLAPLLRQAMDAARVSGEHYSGRWTDVGTPARLAALDEELRRHAA
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]