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The map label for this gene is queD [H]

Identifier: 74318332

GI number: 74318332

Start: 2388888

End: 2389256

Strand: Reverse

Name: queD [H]

Synonym: Tbd_2314

Alternate gene names: 74318332

Gene position: 2389256-2388888 (Counterclockwise)

Preceding gene: 74318333

Following gene: 74318328

Centisome position: 82.11

GC content: 67.21

Gene sequence:

>369_bases
ATGGACATCTTCCGCATCTTTCACCTGCAATGCGCGCGGCGCCTGCCCGCGCTGCCCGCCAGCCACCGCTGCTCGCGCGT
GCACGGGCATTCGTTCGAGGTCGAGCTGACGGTCAGCGGCGAGGTCGACGCGCGTCTTGGCTGGGTCTGCGATTTCGCCG
AGATCGAGGCAGCGTGGCGGCCGATCCACGAGGCGCTCGACCACCGCTACCTCAACGACGTCGACAGACTCGACAATCCG
ACCAGCGAGCGGCTTGCCATCTGGCTGTGGGCGCAGTTGAAGCCGTCCTTGCCCGGCCTGTCACAGATCCGGGTCATGGA
GACCCACGACTCGGGCTGCATCTACCGCGGCGAGCCGACCGCTTCCTAG

Upstream 100 bases:

>100_bases
CCTGCTCAAGCAACTGGCAGCCTAGACGCCCGCGGCCGGCGGCGCACCCCCCGGCCGCGGCACGCATGGTCAGGCCGCGG
CGCATCGGCGAGAATGCGTC

Downstream 100 bases:

>100_bases
CGCGGCGGCGGTCCTTGCCTCAACGTGCGTCGGGGGCCGCAGTGCCGGCCGGCGCATCTGGCAGACGCGTCGCCAGCACC
TTGTCGATGCGCTTGCCGTC

Product: 6-pyruvoyl tetrahydrobiopterin synthase

Products: 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine; Triphosphate. [C]

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MDIFRIFHLQCARRLPALPASHRCSRVHGHSFEVELTVSGEVDARLGWVCDFAEIEAAWRPIHEALDHRYLNDVDRLDNP
TSERLAIWLWAQLKPSLPGLSQIRVMETHDSGCIYRGEPTAS

Sequences:

>Translated_122_residues
MDIFRIFHLQCARRLPALPASHRCSRVHGHSFEVELTVSGEVDARLGWVCDFAEIEAAWRPIHEALDHRYLNDVDRLDNP
TSERLAIWLWAQLKPSLPGLSQIRVMETHDSGCIYRGEPTAS
>Mature_122_residues
MDIFRIFHLQCARRLPALPASHRCSRVHGHSFEVELTVSGEVDARLGWVCDFAEIEAAWRPIHEALDHRYLNDVDRLDNP
TSERLAIWLWAQLKPSLPGLSQIRVMETHDSGCIYRGEPTAS

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=116, Percent_Identity=55.1724137931034, Blast_Score=145, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12 [C]

Molecular weight: Translated: 13960; Mature: 13960

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFRIFHLQCARRLPALPASHRCSRVHGHSFEVELTVSGEVDARLGWVCDFAEIEAAWR
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECHHHHHHHHH
PIHEALDHRYLNDVDRLDNPTSERLAIWLWAQLKPSLPGLSQIRVMETHDSGCIYRGEPT
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCEECCCCC
AS
CC
>Mature Secondary Structure
MDIFRIFHLQCARRLPALPASHRCSRVHGHSFEVELTVSGEVDARLGWVCDFAEIEAAWR
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECHHHHHHHHH
PIHEALDHRYLNDVDRLDNPTSERLAIWLWAQLKPSLPGLSQIRVMETHDSGCIYRGEPT
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCEECCCCC
AS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate [C]

Specific reaction: 2-Amino-4-Oxo-6-(Erythro-1',2',3'- Trihydroxyprolyl)-7,8-Dihydroxypteridine Triphosphate = 2-Amino- 4-Oxo-6-(1',2'-Dioxoprolyl)-7,8-Dihydroxypteridine + Triphosphate. [C]

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]