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The map label for this gene is minD [H]

Identifier: 74318321

GI number: 74318321

Start: 2375709

End: 2376524

Strand: Direct

Name: minD [H]

Synonym: Tbd_2303

Alternate gene names: 74318321

Gene position: 2375709-2376524 (Clockwise)

Preceding gene: 74318320

Following gene: 74318322

Centisome position: 81.64

GC content: 63.97

Gene sequence:

>816_bases
GTGGCAACCATCATCACTGTCACCTCAGGCAAGGGCGGCGTCGGCAAGACGACGACCAGCGCATCGATCGCGAGCGGCCT
CGCGCTGCGCGGCTTCAAGACCGCAGTGATCGACTTCGACGTGGGCCTGCGCAATCTCGACCTCATCATGGGCTGCGAGC
GCCGCGTGGTGTACGACTTCGTCAACGTGATCCAGGGCGACGCCAACCTGAGCCAGGCGCTGATCAAGGACAAACACGCC
GACAACCTGTTCGTGCTGCCGGCCTCGCAGACGCGCGACAAGGACGCGCTGACCGAGGAAGGCGTGGAAAAGGTGTTGAA
GGAGCTCGAACACCAGGGCTTCGACTACATCGTCTGCGATTCGCCGGCCGGCATCGAGCACGGCGCGGTGATGGCGCTGA
CCTTCGCCGACGAGGCGATCATCGTGACCAACCCGGAAGTCTCGTCGGTACGCGACTCCGACCGCATCCTCGGCATCATC
CAGTCGAAGAGCCGGCGCGCGATCGAAGGGCGCGAGCCGGTCAAGGAACACCTGCTCGTCACGCGCTATTCGCCCAAGCG
CGCGGTCGACGGCGAGATGCTGACCTACACGGATATCCAGGAACTGCTGCGGATTCCGCTGCTCGGCGTCATTCCCGAAT
CCGAAGCCGTGCTGCAAGCTTCGAACCAGGGCATTCCCGCGATTCACCAGAAGGACACGCCGGTGGCCGAGGCCTACCTC
GACGCGATCGCGCGTTTCCTCGGCGAACAACGCCCGCTGCGCTTCGTCGATTACGAGAAGCCCGGCCTCATCAAGCGACT
ATTCGGAGGCAAGTGA

Upstream 100 bases:

>100_bases
CGACGCCGCGGTCGCGCGCAAGCCTGCGCAAATCCGCCTGGCCGACGCGAGCCAGATCGTCATCGAACCTCTCTAACCCA
ACCCACCAAAAGGAAGCTCT

Downstream 100 bases:

>100_bases
CATGTCCTGGCTCTCCCTCATCTTCGGCGAAAAGAAATCGACCGCCTCGGTCGCCAAAGAGCGGCTGCAACTCATCATCG
CGCACGAGCGCGTCGGCCTG

Product: septum site-determining protein MinD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MATIITVTSGKGGVGKTTTSASIASGLALRGFKTAVIDFDVGLRNLDLIMGCERRVVYDFVNVIQGDANLSQALIKDKHA
DNLFVLPASQTRDKDALTEEGVEKVLKELEHQGFDYIVCDSPAGIEHGAVMALTFADEAIIVTNPEVSSVRDSDRILGII
QSKSRRAIEGREPVKEHLLVTRYSPKRAVDGEMLTYTDIQELLRIPLLGVIPESEAVLQASNQGIPAIHQKDTPVAEAYL
DAIARFLGEQRPLRFVDYEKPGLIKRLFGGK

Sequences:

>Translated_271_residues
MATIITVTSGKGGVGKTTTSASIASGLALRGFKTAVIDFDVGLRNLDLIMGCERRVVYDFVNVIQGDANLSQALIKDKHA
DNLFVLPASQTRDKDALTEEGVEKVLKELEHQGFDYIVCDSPAGIEHGAVMALTFADEAIIVTNPEVSSVRDSDRILGII
QSKSRRAIEGREPVKEHLLVTRYSPKRAVDGEMLTYTDIQELLRIPLLGVIPESEAVLQASNQGIPAIHQKDTPVAEAYL
DAIARFLGEQRPLRFVDYEKPGLIKRLFGGK
>Mature_270_residues
ATIITVTSGKGGVGKTTTSASIASGLALRGFKTAVIDFDVGLRNLDLIMGCERRVVYDFVNVIQGDANLSQALIKDKHAD
NLFVLPASQTRDKDALTEEGVEKVLKELEHQGFDYIVCDSPAGIEHGAVMALTFADEAIIVTNPEVSSVRDSDRILGIIQ
SKSRRAIEGREPVKEHLLVTRYSPKRAVDGEMLTYTDIQELLRIPLLGVIPESEAVLQASNQGIPAIHQKDTPVAEAYLD
AIARFLGEQRPLRFVDYEKPGLIKRLFGGK

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=270, Percent_Identity=70.3703703703704, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29599; Mature: 29468

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIITVTSGKGGVGKTTTSASIASGLALRGFKTAVIDFDVGLRNLDLIMGCERRVVYDF
CEEEEEEECCCCCCCCCCCHHHHHHCHHHCCCEEEEEEECCCCCHHHHHHCCCHHHHHHH
VNVIQGDANLSQALIKDKHADNLFVLPASQTRDKDALTEEGVEKVLKELEHQGFDYIVCD
HHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
SPAGIEHGAVMALTFADEAIIVTNPEVSSVRDSDRILGIIQSKSRRAIEGREPVKEHLLV
CCCCCCCCEEEEEEECCCEEEEECCCHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCEEE
TRYSPKRAVDGEMLTYTDIQELLRIPLLGVIPESEAVLQASNQGIPAIHQKDTPVAEAYL
EECCCCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHH
DAIARFLGEQRPLRFVDYEKPGLIKRLFGGK
HHHHHHHCCCCCCEEECCCCCCHHHHHHCCC
>Mature Secondary Structure 
ATIITVTSGKGGVGKTTTSASIASGLALRGFKTAVIDFDVGLRNLDLIMGCERRVVYDF
EEEEEEECCCCCCCCCCCHHHHHHCHHHCCCEEEEEEECCCCCHHHHHHCCCHHHHHHH
VNVIQGDANLSQALIKDKHADNLFVLPASQTRDKDALTEEGVEKVLKELEHQGFDYIVCD
HHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
SPAGIEHGAVMALTFADEAIIVTNPEVSSVRDSDRILGIIQSKSRRAIEGREPVKEHLLV
CCCCCCCCEEEEEEECCCEEEEECCCHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCEEE
TRYSPKRAVDGEMLTYTDIQELLRIPLLGVIPESEAVLQASNQGIPAIHQKDTPVAEAYL
EECCCCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHH
DAIARFLGEQRPLRFVDYEKPGLIKRLFGGK
HHHHHHHCCCCCCEEECCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]