Click here to switch to the map view.

The map label for this gene is trpE [H]

Identifier: 74318246

GI number: 74318246

Start: 2307504

End: 2309069

Strand: Reverse

Name: trpE [H]

Synonym: Tbd_2228

Alternate gene names: 74318246

Gene position: 2309069-2307504 (Counterclockwise)

Preceding gene: 74318247

Following gene: 74318244

Centisome position: 79.35

GC content: 67.11

Gene sequence:

>1566_bases
ATGGCGGCGCTCGTCCTGACAAGACGCGCAGCACATTCCCTGACCCCTGAATCCCGGATCCCGACCCCTGCCATGACCGA
ACAAGAATTCTTCGACCTTGCCCGCCAGGGCTTCAACCGTATCCCGCTGGTGCGCGAACTGCCCGGCGACCTCGAGACGC
CGCTGTCGGTCTATCTCAAGCTCGCCAACGCGCCCTATACCTATCTCCTCGAATCGGTCGTCGGCGGCGAACGCTTCGGA
CGCTATTCCTTCATCGGCCTGCCGGCGCGCACCGTGCTCAGGGTGCGCGCCCACCTCCTGACGGTCGAAACCGACGGCCA
GGTCGTCGAGGAATGCAGCACGCCCGATCCGCTCGCTTTCATCGCCGAGTATCAGACCCGCTTCAAGGCCGCGCCGGTCC
TGGGCTTGCCGCGCTTCACCGGCGGCCTGGCCGGCTACTTCGGCTACGACACGATCCGTTACATCGAGAAGCGACTGACG
CCGGAATTCAACAAGAACCTCGTGCGCAAGGACGACGTCCTGCACACGCCCGACATCCTGCTGATGCTGACCGAGGAACT
CGCCGTCTTCGACAACCTCGCCGGCAAGCTCTATCTCGTCACCCACGCCGACCCGATGCAGGCGGACGCCTATGCGCAGG
GGCACTTGCGGCTCGCGGAGCTTGCCGAGAAGCTGCACGCGCCGGTCACGCTGCCGAGCGAGCCGCGCCCGGTCGTCGCC
GAAGCGAGCTCCGAATTCGGCGAAGCCGAGTTCAAGGCCGCGGTTGCCAAGGCCAAGGAGTACATCGCGGCCGGCGACGT
CATGCAGGTCGTGCTGTCGCAGCGGATGGCGCGGCCGTTCGAGGCCTCGCCGCTGTCGCTCTACCGTGCGCTGCGCAGCC
TCAACCCGTCGCCCTACATGTATTTCTACGACCTTGGCGGCTTCCACATCGTCGGCTCGTCGCCCGAGATCCTGGTCCGG
CTCGAGGGCGACACGGTCACGCTGCGGCCGATCGCCGGCACGCGGCCGCGCGGGCTCACGCGCGAGGACGATCAGCGCCT
CGCGGCCGAATTGATGGCCGACCCGAAGGAATGCGCCGAACATCTGCAGCTCCTCGACCTCGGGCGCAACGACACGGGGC
GGGTCGCCGTGACCGGCAGCGTCAAGGTCACGGAACACATGCAGATCGAGCGCTATTCGCACGTCATGCACATCGTCTCG
AACGTCGAAGGCAAGCTCAAGCCCGGACTCTCCGCGCTCGACGTCCTGCGCGCGAGCTTTCCGGCAGGTACGGTCTCGGG
GGCGCCCAAGGTGCGGGCGATGGAGATCATCGACGAGCTCGAACCGAGCAAGCGCGGGGTCTACGCCGGCGCCGTCGGCT
ACCTCGGCTTCAGCGGCGACATGGATCTCGCGATCGCGATCCGCACGGCCGTCGTCAAGGATGGAATGCTCTACGCCCAG
GCCGGCGCCGGCATCGTCGCCGACTCGGTCCCCGACAACGAGTGGACCGAGACGCTCAACAAGGCGCGGGCGGTCGTGCG
TGCGGCCGAGCTCGCCTACGCGCGCTTCGGCGACGTCGTCGAATAA

Upstream 100 bases:

>100_bases
GCAGTCGTGCCGAGTCTCGCCGAGGCGGCGCTGATGGTGACGAAGGCGTGATGGCAAGGGGGCGGGATTCGGGGATCAGG
ATTCAGGGGCCGTGCCGCCG

Downstream 100 bases:

>100_bases
GGCGTTTGCCCGGCCCGCCGAAACGCGCGGGCGCTAACTCAGGCCGTAGCGTTTGAGCTTGCGGTACAGCGTGCGCTCGG
TGACGCCGAGTGCCTGGGCG

Product: anthranilate synthase, component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MAALVLTRRAAHSLTPESRIPTPAMTEQEFFDLARQGFNRIPLVRELPGDLETPLSVYLKLANAPYTYLLESVVGGERFG
RYSFIGLPARTVLRVRAHLLTVETDGQVVEECSTPDPLAFIAEYQTRFKAAPVLGLPRFTGGLAGYFGYDTIRYIEKRLT
PEFNKNLVRKDDVLHTPDILLMLTEELAVFDNLAGKLYLVTHADPMQADAYAQGHLRLAELAEKLHAPVTLPSEPRPVVA
EASSEFGEAEFKAAVAKAKEYIAAGDVMQVVLSQRMARPFEASPLSLYRALRSLNPSPYMYFYDLGGFHIVGSSPEILVR
LEGDTVTLRPIAGTRPRGLTREDDQRLAAELMADPKECAEHLQLLDLGRNDTGRVAVTGSVKVTEHMQIERYSHVMHIVS
NVEGKLKPGLSALDVLRASFPAGTVSGAPKVRAMEIIDELEPSKRGVYAGAVGYLGFSGDMDLAIAIRTAVVKDGMLYAQ
AGAGIVADSVPDNEWTETLNKARAVVRAAELAYARFGDVVE

Sequences:

>Translated_521_residues
MAALVLTRRAAHSLTPESRIPTPAMTEQEFFDLARQGFNRIPLVRELPGDLETPLSVYLKLANAPYTYLLESVVGGERFG
RYSFIGLPARTVLRVRAHLLTVETDGQVVEECSTPDPLAFIAEYQTRFKAAPVLGLPRFTGGLAGYFGYDTIRYIEKRLT
PEFNKNLVRKDDVLHTPDILLMLTEELAVFDNLAGKLYLVTHADPMQADAYAQGHLRLAELAEKLHAPVTLPSEPRPVVA
EASSEFGEAEFKAAVAKAKEYIAAGDVMQVVLSQRMARPFEASPLSLYRALRSLNPSPYMYFYDLGGFHIVGSSPEILVR
LEGDTVTLRPIAGTRPRGLTREDDQRLAAELMADPKECAEHLQLLDLGRNDTGRVAVTGSVKVTEHMQIERYSHVMHIVS
NVEGKLKPGLSALDVLRASFPAGTVSGAPKVRAMEIIDELEPSKRGVYAGAVGYLGFSGDMDLAIAIRTAVVKDGMLYAQ
AGAGIVADSVPDNEWTETLNKARAVVRAAELAYARFGDVVE
>Mature_520_residues
AALVLTRRAAHSLTPESRIPTPAMTEQEFFDLARQGFNRIPLVRELPGDLETPLSVYLKLANAPYTYLLESVVGGERFGR
YSFIGLPARTVLRVRAHLLTVETDGQVVEECSTPDPLAFIAEYQTRFKAAPVLGLPRFTGGLAGYFGYDTIRYIEKRLTP
EFNKNLVRKDDVLHTPDILLMLTEELAVFDNLAGKLYLVTHADPMQADAYAQGHLRLAELAEKLHAPVTLPSEPRPVVAE
ASSEFGEAEFKAAVAKAKEYIAAGDVMQVVLSQRMARPFEASPLSLYRALRSLNPSPYMYFYDLGGFHIVGSSPEILVRL
EGDTVTLRPIAGTRPRGLTREDDQRLAAELMADPKECAEHLQLLDLGRNDTGRVAVTGSVKVTEHMQIERYSHVMHIVSN
VEGKLKPGLSALDVLRASFPAGTVSGAPKVRAMEIIDELEPSKRGVYAGAVGYLGFSGDMDLAIAIRTAVVKDGMLYAQA
GAGIVADSVPDNEWTETLNKARAVVRAAELAYARFGDVVE

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=392, Percent_Identity=40.0510204081633, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788114, Length=456, Percent_Identity=32.6754385964912, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786809, Length=254, Percent_Identity=29.9212598425197, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87082077, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=504, Percent_Identity=39.484126984127, Blast_Score=319, Evalue=8e-88,
Organism=Saccharomyces cerevisiae, GI6324361, Length=500, Percent_Identity=25.6, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57070; Mature: 56939

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALVLTRRAAHSLTPESRIPTPAMTEQEFFDLARQGFNRIPLVRELPGDLETPLSVYLK
CCCEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHH
LANAPYTYLLESVVGGERFGRYSFIGLPARTVLRVRAHLLTVETDGQVVEECSTPDPLAF
HCCCCHHHHHHHHHCCHHCCCEEEECCCHHHHHHHHHHEEEEECCCHHHHHCCCCCCHHH
IAEYQTRFKAAPVLGLPRFTGGLAGYFGYDTIRYIEKRLTPEFNKNLVRKDDVLHTPDIL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHH
LMLTEELAVFDNLAGKLYLVTHADPMQADAYAQGHLRLAELAEKLHAPVTLPSEPRPVVA
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
EASSEFGEAEFKAAVAKAKEYIAAGDVMQVVLSQRMARPFEASPLSLYRALRSLNPSPYM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEE
YFYDLGGFHIVGSSPEILVRLEGDTVTLRPIAGTRPRGLTREDDQRLAAELMADPKECAE
EEEECCCEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
HLQLLDLGRNDTGRVAVTGSVKVTEHMQIERYSHVMHIVSNVEGKLKPGLSALDVLRASF
HHHHHHCCCCCCCCEEEEECEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
PAGTVSGAPKVRAMEIIDELEPSKRGVYAGAVGYLGFSGDMDLAIAIRTAVVKDGMLYAQ
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEHHEEEECCCCCCEEEEHHHHHHHCCEEEEE
AGAGIVADSVPDNEWTETLNKARAVVRAAELAYARFGDVVE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AALVLTRRAAHSLTPESRIPTPAMTEQEFFDLARQGFNRIPLVRELPGDLETPLSVYLK
CCEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHH
LANAPYTYLLESVVGGERFGRYSFIGLPARTVLRVRAHLLTVETDGQVVEECSTPDPLAF
HCCCCHHHHHHHHHCCHHCCCEEEECCCHHHHHHHHHHEEEEECCCHHHHHCCCCCCHHH
IAEYQTRFKAAPVLGLPRFTGGLAGYFGYDTIRYIEKRLTPEFNKNLVRKDDVLHTPDIL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHH
LMLTEELAVFDNLAGKLYLVTHADPMQADAYAQGHLRLAELAEKLHAPVTLPSEPRPVVA
HHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
EASSEFGEAEFKAAVAKAKEYIAAGDVMQVVLSQRMARPFEASPLSLYRALRSLNPSPYM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEE
YFYDLGGFHIVGSSPEILVRLEGDTVTLRPIAGTRPRGLTREDDQRLAAELMADPKECAE
EEEECCCEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
HLQLLDLGRNDTGRVAVTGSVKVTEHMQIERYSHVMHIVSNVEGKLKPGLSALDVLRASF
HHHHHHCCCCCCCCEEEEECEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
PAGTVSGAPKVRAMEIIDELEPSKRGVYAGAVGYLGFSGDMDLAIAIRTAVVKDGMLYAQ
CCCCCCCCCCHHHHHHHHHCCCCCCCEEEEHHEEEECCCCCCEEEEHHHHHHHCCEEEEE
AGAGIVADSVPDNEWTETLNKARAVVRAAELAYARFGDVVE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987141 [H]