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The map label for this gene is ribH

Identifier: 74318209

GI number: 74318209

Start: 2273180

End: 2273647

Strand: Direct

Name: ribH

Synonym: Tbd_2191

Alternate gene names: 74318209

Gene position: 2273180-2273647 (Clockwise)

Preceding gene: 74318208

Following gene: 74318210

Centisome position: 78.12

GC content: 65.38

Gene sequence:

>468_bases
ATGGGAACCAGCAGCGACATTTTTGAGCTCGACCCGATCTACCAGGGCAAGGGGCTCAGGATCGGCATCGTCATGAGCCG
CTTCAACAAGGACATCTGCGAAGGCCTTCTGTCGGCCTGCGAGACCGAGCTCGACCGTCTCGGGGTCGCACGCGAGGACG
TGCTGCTCGTCGACGTGCCCGGCGCACTCGAACATCCGCTCGCGCTGCAGAAAATGGCGCAGTCCGCCCGCTACGACGCC
CTGATCGCGATCGGCGCCGTCGTGCGCGGCGACACCTACCATTTCGAGATCGTGTCGAACGAGTCCTCGCGCGGCATCCT
CGACGTCCAGCTCGAAACCGGCGTGCCGATCGCCAACGCGATCCTCACCGTCGACACCGAGGAGCAGGCGCACGTGCGCA
TGGCCGAAAAAGGCCGCGACGCCGCGCGCGTAGCGGTCGAGATGGCCAACCTGCTGAAGCGTCTCTGA

Upstream 100 bases:

>100_bases
TGAAGCTGCTCGCCAGCCCGCGCAAGATGCCGGCGATGGACGGCTTCGGCCTGGAAGTCACCGGCTATTCTGAAAAAGCA
TAATCAACGAAGGCACGACC

Downstream 100 bases:

>100_bases
TGGCCGGCTCCCGCAAGGTCGCGCGCGAATTCACGCTGCAGGGCATCTACGCCTGGATGATCGGCGGCGCCGACGTCACG
CTCATCGCCGCGAATCTCAA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MGTSSDIFELDPIYQGKGLRIGIVMSRFNKDICEGLLSACETELDRLGVAREDVLLVDVPGALEHPLALQKMAQSARYDA
LIAIGAVVRGDTYHFEIVSNESSRGILDVQLETGVPIANAILTVDTEEQAHVRMAEKGRDAARVAVEMANLLKRL

Sequences:

>Translated_155_residues
MGTSSDIFELDPIYQGKGLRIGIVMSRFNKDICEGLLSACETELDRLGVAREDVLLVDVPGALEHPLALQKMAQSARYDA
LIAIGAVVRGDTYHFEIVSNESSRGILDVQLETGVPIANAILTVDTEEQAHVRMAEKGRDAARVAVEMANLLKRL
>Mature_154_residues
GTSSDIFELDPIYQGKGLRIGIVMSRFNKDICEGLLSACETELDRLGVAREDVLLVDVPGALEHPLALQKMAQSARYDAL
IAIGAVVRGDTYHFEIVSNESSRGILDVQLETGVPIANAILTVDTEEQAHVRMAEKGRDAARVAVEMANLLKRL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=40.4255319148936, Blast_Score=104, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324429, Length=152, Percent_Identity=30.2631578947368, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_THIDA (Q3SGU9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315949.1
- HSSP:   Q9UUB1
- ProteinModelPortal:   Q3SGU9
- SMR:   Q3SGU9
- STRING:   Q3SGU9
- GeneID:   3671972
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2191
- NMPDR:   fig|292415.3.peg.2080
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q3SGU9
- ProtClustDB:   PRK00061
- BioCyc:   TDEN292415:TBD_2191-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16878; Mature: 16746

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTSSDIFELDPIYQGKGLRIGIVMSRFNKDICEGLLSACETELDRLGVAREDVLLVDVP
CCCCCCCEEECCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
GALEHPLALQKMAQSARYDALIAIGAVVRGDTYHFEIVSNESSRGILDVQLETGVPIANA
CCHHCCHHHHHHHHHCCHHHHEEEHHEEECCEEEEEEEECCCCCCEEEEEECCCCCEEHE
ILTVDTEEQAHVRMAEKGRDAARVAVEMANLLKRL
EEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTSSDIFELDPIYQGKGLRIGIVMSRFNKDICEGLLSACETELDRLGVAREDVLLVDVP
CCCCCCEEECCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
GALEHPLALQKMAQSARYDALIAIGAVVRGDTYHFEIVSNESSRGILDVQLETGVPIANA
CCHHCCHHHHHHHHHCCHHHHEEEHHEEECCEEEEEEEECCCCCCEEEEEECCCCCEEHE
ILTVDTEEQAHVRMAEKGRDAARVAVEMANLLKRL
EEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA