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The map label for this gene is rna [H]

Identifier: 74318151

GI number: 74318151

Start: 2219999

End: 2220838

Strand: Reverse

Name: rna [H]

Synonym: Tbd_2133

Alternate gene names: 74318151

Gene position: 2220838-2219999 (Counterclockwise)

Preceding gene: 74318159

Following gene: 74318146

Centisome position: 76.32

GC content: 68.33

Gene sequence:

>840_bases
ATGGAAAGCACGAATTTTACCGGAATCTCCGGGGCGCGAAGCGCGCGCCTGCGCGATGTTTTCGCGGTCTGGAGCGCTGG
CCGCGCCGGCCGGACTGCCGGAAGATGGCTCGCGCTTGGTCTCCTGCTGTGGAGCGTTTCCGTCCGCGCCGAGGCGCCGC
GTTTCGACTTCTATCTGCTCGCGCTGAGCGTCGCGCCGGCGTTCTGCGAGGACGCGCCGCAACGCAAGCGCGGCTTCGCG
CAATGCAAGGCGCTCAGCGAGCGCGACTTCAAGCGGGTGCCCCTGACGCTGCACGGGCTGTGGCCGAACCGCGAGGATCG
CCGGCACCCGGCCTATTGCGCGGGAAAATCCGCGCGAGCGTTCTGCGCGCTGCCCGCGCCTGCGCTGCCCTCGGAAACAG
AGGCCGCGTTGCGGCAGTTCATGCCTGCGACTGCGGATTGCCTCGAGCGCCACCAGTGGGCCAAGCACGGCAGCTGCTCG
GGTCTGGCCCCGGCCGAGTATTTCTCCGCGTCTGCGGCGTTGAGCGCGCGGGTGAACCGGGCGATCGGCGACGAGCTCGC
GCGGCACATGGGACGCGAGGTGGATCTGGCGCTATTGCGTGAGCGCTTGCGTGAAAACGATCGTTCGCTCGAACAAGCGG
TCGTATTCGAATGCCGCACGCCGCGCACGCCGGACCCGGCCAAGCGCCGCCCGATGCTGCGCGAAGTCCACGTCTATTTC
AACCGGGACCCAGTGACGGGCGCACCCGGCCAGCCGCTTCCCTATGTTCGTGCCGGCGCGAAACACTACAATTCCGGCTG
CCCTCGAGGACGCGCCTACGTCGACAGCCCGCTTGACTGA

Upstream 100 bases:

>100_bases
GACGTGGGTGGTAGCGTCGCGCCATGGTACGAGTTTGCCGTCGTACCATATGACGCCGTCGCGGTCGGCCATGGACATGG
TGAGAAGTCTCTGTGAACGA

Downstream 100 bases:

>100_bases
GGCGGCGGCTCAGGGCGTGTTGAGGCGAACGAACTCGGGAAAGTATTCGGCGCGAACGATATAGGTCCCGTCGCAGTTGT
AGGATGCGCGGACGAGGCCC

Product: putative ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MESTNFTGISGARSARLRDVFAVWSAGRAGRTAGRWLALGLLLWSVSVRAEAPRFDFYLLALSVAPAFCEDAPQRKRGFA
QCKALSERDFKRVPLTLHGLWPNREDRRHPAYCAGKSARAFCALPAPALPSETEAALRQFMPATADCLERHQWAKHGSCS
GLAPAEYFSASAALSARVNRAIGDELARHMGREVDLALLRERLRENDRSLEQAVVFECRTPRTPDPAKRRPMLREVHVYF
NRDPVTGAPGQPLPYVRAGAKHYNSGCPRGRAYVDSPLD

Sequences:

>Translated_279_residues
MESTNFTGISGARSARLRDVFAVWSAGRAGRTAGRWLALGLLLWSVSVRAEAPRFDFYLLALSVAPAFCEDAPQRKRGFA
QCKALSERDFKRVPLTLHGLWPNREDRRHPAYCAGKSARAFCALPAPALPSETEAALRQFMPATADCLERHQWAKHGSCS
GLAPAEYFSASAALSARVNRAIGDELARHMGREVDLALLRERLRENDRSLEQAVVFECRTPRTPDPAKRRPMLREVHVYF
NRDPVTGAPGQPLPYVRAGAKHYNSGCPRGRAYVDSPLD
>Mature_279_residues
MESTNFTGISGARSARLRDVFAVWSAGRAGRTAGRWLALGLLLWSVSVRAEAPRFDFYLLALSVAPAFCEDAPQRKRGFA
QCKALSERDFKRVPLTLHGLWPNREDRRHPAYCAGKSARAFCALPAPALPSETEAALRQFMPATADCLERHQWAKHGSCS
GLAPAEYFSASAALSARVNRAIGDELARHMGREVDLALLRERLRENDRSLEQAVVFECRTPRTPDPAKRRPMLREVHVYF
NRDPVTGAPGQPLPYVRAGAKHYNSGCPRGRAYVDSPLD

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=219, Percent_Identity=31.0502283105023, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 30958; Mature: 30958

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTNFTGISGARSARLRDVFAVWSAGRAGRTAGRWLALGLLLWSVSVRAEAPRFDFYLL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ALSVAPAFCEDAPQRKRGFAQCKALSERDFKRVPLTLHGLWPNREDRRHPAYCAGKSARA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCE
FCALPAPALPSETEAALRQFMPATADCLERHQWAKHGSCSGLAPAEYFSASAALSARVNR
EEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AIGDELARHMGREVDLALLRERLRENDRSLEQAVVFECRTPRTPDPAKRRPMLREVHVYF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHEEE
NRDPVTGAPGQPLPYVRAGAKHYNSGCPRGRAYVDSPLD
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MESTNFTGISGARSARLRDVFAVWSAGRAGRTAGRWLALGLLLWSVSVRAEAPRFDFYLL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ALSVAPAFCEDAPQRKRGFAQCKALSERDFKRVPLTLHGLWPNREDRRHPAYCAGKSARA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCCCCCE
FCALPAPALPSETEAALRQFMPATADCLERHQWAKHGSCSGLAPAEYFSASAALSARVNR
EEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AIGDELARHMGREVDLALLRERLRENDRSLEQAVVFECRTPRTPDPAKRRPMLREVHVYF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHEEE
NRDPVTGAPGQPLPYVRAGAKHYNSGCPRGRAYVDSPLD
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]