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The map label for this gene is proS [H]

Identifier: 74318130

GI number: 74318130

Start: 2200570

End: 2202309

Strand: Direct

Name: proS [H]

Synonym: Tbd_2112

Alternate gene names: 74318130

Gene position: 2200570-2202309 (Clockwise)

Preceding gene: 74318117

Following gene: 74318131

Centisome position: 75.63

GC content: 66.84

Gene sequence:

>1740_bases
TTGTCCACCATGCGCACCACCCAGTTTTTCCTGTCCACCACCAAGGAAGCCCCGTCCGAAGCCGAACTCGTCAGCCACAA
GCTGATGCTGCGCGCCGGGCTGATCAAGCGTCTGGGCTCCGGGCTCTACACCTGGATGCCTCTGGGCCTCCGCGTGCTGC
GCCGGGTCGAGGCCGTGGTCCGCGAGGAGATGAACCGCGCCGGTGCGATCGAGCTGCTGATGCCGGCGGTGCAGCCAGCC
GAGCTGTGGCAGGAAACCGGGCGCTGGGCGCAGTTCGGGCCACAGATGCTCAAGATCAAGGACCGCCACGAACGCGAATT
CTGCTTCGGACCGACCCACGAGGAGGTCATCACCGACCTTGCCCGGCGCGAAATCAAGAGCTACCGGCAGTTGCCGCTCA
ACTTCTACCAGATCCAGACCAAGTTCCGCGACGAGATCCGGCCGCGCTTCGGGGTCATGCGCGCCCGCGAGTTCCTGATG
AAGGACGCCTACTCCTTCCACGCGAGCCGCGAGAGTCTGGCCGCCACCTACCAGGCGATGTACGACGCCTACGGGCGGAT
CTTCACCCGCCTCGGCCTGCGGTTCCGGGCGGTCGCCGCCGACACCGGCGCGATAGGCGGCTCGGCCTCGCACGAATTCC
ACGTGCTCGCCGATTCCGGCGAGGATCTGATCGCCTACTGCCCGGACTCCGATTACGCCGCCAACGTCGAACTTGCCGAG
GCGCTCGCCCCGGCGGCTGCCCGCGGCGCGCCGCAGGAGGCGATGCGCGAGGTCGAGACGCCCAAACAGACGACCTGCGA
GGACGTCGCCGCGCTGCTCGGCATCCCGCTCGCCCGCACCGTGAAGCTCATCGCCGTGATGGCGGGGGAGCGGATGGTCG
TCGTGCTGCTCCGCGGCGACCACATGCTGAACGAGGTGAAACTGGCCAAGATCGAGGGCCTGGCCGATTTCCGCCTCGCG
AACGAAGCCGAAATCCGCGCCGTCTTCGACTGCCCGCCCGGGTTTCTCGGCCCGGTCGGCATCGACCGCTCGACGATCCG
GGTCATCGCGGACCGTGCCGTCGCGGTCATGAGCGACTTCGTCTGCGGCGCCAACAAGCCGAAATTCCACCTCGCCGGCG
TCAATTTCGGCCGCGACCTGCAGGAACCCGATCTCGTGGCCGATATAAGGAATGTCGTCGCCGGCGACCCGAGTCCGGAC
GGCAAAGGCACGCTCGCGCTGTGCCGCGGCATCGAAGTCGGCCACGTGTTCCAGCTCGGCAACAAGTACTCGCAGGCGAT
GAACGCAACCTACCTCGACGAGGCGGGAAAGGCGCAGGCGATGGAGATGGGCTGCTATGGCATCGGCGTGTCGCGCATCG
TGGCCGCCGCGGTCGAGCAGAACCACGACGGCAAAGGCATCGTCTGGCCGGCGTCGATGGCGCCCTTCTCGGTCGTCATT
GTCGCCATCGGCTACGGCAAGAGCGCCAGCGTTAAAAATGCCGCGGATACGCTCTATGCTGATCTGATGGCGGTCGGGGT
CGAAGTCCTGCTCGACGACCGCGACGAACGCCCCGGCGTGATGTTCGCCGACGCCGAACTGGTCGGCATCCCTCACCGCG
TCACGCTCGGCGAGCGCGGCCTGAATGAGGGAGTCGTGGAATATCAGCCGCGCCGCGCCGCGCCGGAACAGGGCGCCGAT
GCGCGGAAAATCGCGGTCGCCGAAGCAAAGGCGTTCTTGATGGGTGTATTGGGAGACTGA

Upstream 100 bases:

>100_bases
CCCGACGTCTCCCAAACCGCAATCGCATTGCCATTTCCACCTCGAATCGAGCGCGGAAGATTACACGGCCGCACGCTAAA
ATGCCGCTTTTCCTCCCGGC

Downstream 100 bases:

>100_bases
CATGCGGAAAACGACGCGGGGCCTGCTGATCGCGGCCATGCTGCTGGCCTCGGCGGCCCACGCCGGCGGCCAGCGCGAGG
AGGCGTTGGCGGCCAACGTC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MSTMRTTQFFLSTTKEAPSEAELVSHKLMLRAGLIKRLGSGLYTWMPLGLRVLRRVEAVVREEMNRAGAIELLMPAVQPA
ELWQETGRWAQFGPQMLKIKDRHEREFCFGPTHEEVITDLARREIKSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFLM
KDAYSFHASRESLAATYQAMYDAYGRIFTRLGLRFRAVAADTGAIGGSASHEFHVLADSGEDLIAYCPDSDYAANVELAE
ALAPAAARGAPQEAMREVETPKQTTCEDVAALLGIPLARTVKLIAVMAGERMVVVLLRGDHMLNEVKLAKIEGLADFRLA
NEAEIRAVFDCPPGFLGPVGIDRSTIRVIADRAVAVMSDFVCGANKPKFHLAGVNFGRDLQEPDLVADIRNVVAGDPSPD
GKGTLALCRGIEVGHVFQLGNKYSQAMNATYLDEAGKAQAMEMGCYGIGVSRIVAAAVEQNHDGKGIVWPASMAPFSVVI
VAIGYGKSASVKNAADTLYADLMAVGVEVLLDDRDERPGVMFADAELVGIPHRVTLGERGLNEGVVEYQPRRAAPEQGAD
ARKIAVAEAKAFLMGVLGD

Sequences:

>Translated_579_residues
MSTMRTTQFFLSTTKEAPSEAELVSHKLMLRAGLIKRLGSGLYTWMPLGLRVLRRVEAVVREEMNRAGAIELLMPAVQPA
ELWQETGRWAQFGPQMLKIKDRHEREFCFGPTHEEVITDLARREIKSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFLM
KDAYSFHASRESLAATYQAMYDAYGRIFTRLGLRFRAVAADTGAIGGSASHEFHVLADSGEDLIAYCPDSDYAANVELAE
ALAPAAARGAPQEAMREVETPKQTTCEDVAALLGIPLARTVKLIAVMAGERMVVVLLRGDHMLNEVKLAKIEGLADFRLA
NEAEIRAVFDCPPGFLGPVGIDRSTIRVIADRAVAVMSDFVCGANKPKFHLAGVNFGRDLQEPDLVADIRNVVAGDPSPD
GKGTLALCRGIEVGHVFQLGNKYSQAMNATYLDEAGKAQAMEMGCYGIGVSRIVAAAVEQNHDGKGIVWPASMAPFSVVI
VAIGYGKSASVKNAADTLYADLMAVGVEVLLDDRDERPGVMFADAELVGIPHRVTLGERGLNEGVVEYQPRRAAPEQGAD
ARKIAVAEAKAFLMGVLGD
>Mature_578_residues
STMRTTQFFLSTTKEAPSEAELVSHKLMLRAGLIKRLGSGLYTWMPLGLRVLRRVEAVVREEMNRAGAIELLMPAVQPAE
LWQETGRWAQFGPQMLKIKDRHEREFCFGPTHEEVITDLARREIKSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMK
DAYSFHASRESLAATYQAMYDAYGRIFTRLGLRFRAVAADTGAIGGSASHEFHVLADSGEDLIAYCPDSDYAANVELAEA
LAPAAARGAPQEAMREVETPKQTTCEDVAALLGIPLARTVKLIAVMAGERMVVVLLRGDHMLNEVKLAKIEGLADFRLAN
EAEIRAVFDCPPGFLGPVGIDRSTIRVIADRAVAVMSDFVCGANKPKFHLAGVNFGRDLQEPDLVADIRNVVAGDPSPDG
KGTLALCRGIEVGHVFQLGNKYSQAMNATYLDEAGKAQAMEMGCYGIGVSRIVAAAVEQNHDGKGIVWPASMAPFSVVIV
AIGYGKSASVKNAADTLYADLMAVGVEVLLDDRDERPGVMFADAELVGIPHRVTLGERGLNEGVVEYQPRRAAPEQGADA
RKIAVAEAKAFLMGVLGD

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.4601769911504, Blast_Score=221, Evalue=2e-57,
Organism=Escherichia coli, GI1786392, Length=559, Percent_Identity=59.9284436493739, Blast_Score=692, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=244, Percent_Identity=35.655737704918, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=93, Percent_Identity=30.1075268817204, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=557, Percent_Identity=30.3411131059246, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24656200, Length=235, Percent_Identity=40.4255319148936, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63275; Mature: 63143

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMRTTQFFLSTTKEAPSEAELVSHKLMLRAGLIKRLGSGLYTWMPLGLRVLRRVEAVV
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
REEMNRAGAIELLMPAVQPAELWQETGRWAQFGPQMLKIKDRHEREFCFGPTHEEVITDL
HHHHHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHEEECCCCCCCCCCCCCHHHHHHHH
ARREIKSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDAYSFHASRESLAATYQAM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDAYGRIFTRLGLRFRAVAADTGAIGGSASHEFHVLADSGEDLIAYCPDSDYAANVELAE
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHH
ALAPAAARGAPQEAMREVETPKQTTCEDVAALLGIPLARTVKLIAVMAGERMVVVLLRGD
HHCCHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCC
HMLNEVKLAKIEGLADFRLANEAEIRAVFDCPPGFLGPVGIDRSTIRVIADRAVAVMSDF
CHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VCGANKPKFHLAGVNFGRDLQEPDLVADIRNVVAGDPSPDGKGTLALCRGIEVGHVFQLG
HCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHH
NKYSQAMNATYLDEAGKAQAMEMGCYGIGVSRIVAAAVEQNHDGKGIVWPASMAPFSVVI
HHHHHHHCCHHHHCCCCHHHHHHCHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
VAIGYGKSASVKNAADTLYADLMAVGVEVLLDDRDERPGVMFADAELVGIPHRVTLGERG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCEEECCCCEEECCCCC
LNEGVVEYQPRRAAPEQGADARKIAVAEAKAFLMGVLGD
CCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STMRTTQFFLSTTKEAPSEAELVSHKLMLRAGLIKRLGSGLYTWMPLGLRVLRRVEAVV
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
REEMNRAGAIELLMPAVQPAELWQETGRWAQFGPQMLKIKDRHEREFCFGPTHEEVITDL
HHHHHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHEEECCCCCCCCCCCCCHHHHHHHH
ARREIKSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDAYSFHASRESLAATYQAM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDAYGRIFTRLGLRFRAVAADTGAIGGSASHEFHVLADSGEDLIAYCPDSDYAANVELAE
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHH
ALAPAAARGAPQEAMREVETPKQTTCEDVAALLGIPLARTVKLIAVMAGERMVVVLLRGD
HHCCHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCC
HMLNEVKLAKIEGLADFRLANEAEIRAVFDCPPGFLGPVGIDRSTIRVIADRAVAVMSDF
CHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VCGANKPKFHLAGVNFGRDLQEPDLVADIRNVVAGDPSPDGKGTLALCRGIEVGHVFQLG
HCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHH
NKYSQAMNATYLDEAGKAQAMEMGCYGIGVSRIVAAAVEQNHDGKGIVWPASMAPFSVVI
HHHHHHHCCHHHHCCCCHHHHHHCHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
VAIGYGKSASVKNAADTLYADLMAVGVEVLLDDRDERPGVMFADAELVGIPHRVTLGERG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCEEECCCCEEECCCCC
LNEGVVEYQPRRAAPEQGADARKIAVAEAKAFLMGVLGD
CCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA