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The map label for this gene is pdxJ

Identifier: 74318101

GI number: 74318101

Start: 2176259

End: 2176999

Strand: Reverse

Name: pdxJ

Synonym: Tbd_2083

Alternate gene names: 74318101

Gene position: 2176999-2176259 (Counterclockwise)

Preceding gene: 74318102

Following gene: 74318091

Centisome position: 74.82

GC content: 68.02

Gene sequence:

>741_bases
ATGGGCGGTGCAATGAGCATCTACCTCGGCGTCAACATCGACCACATCGCGACGCTGCGCCAGGCGCGCAAGACGCGTTA
TCCGAGTCCGGTCGAAGCCGCGCTCGCGGCCGAGACCGCGGGCGCCGATTCGATCACCCTGCATCTGCGCGAAGACCGGC
GCCACATCCAGGACGCCGACGTCACGATCCTGCGGCAGACGCTGAAGACGAAAATGAACCTCGAGATGGCGGTGACCGAA
GAGATGCTCGGCATCGCGCTCGAGACCCGGCCTTCGGACGTGTGTCTGGTGCCGGAAAAGCGCGAGGAGCTGACGACCGA
GGGCGGTCTCGATGTGGCGGGGCAGCAAGCGAAGATGCGCGACGCCTGTGCACGCCTGGGCGACGCGGGTATCCGCGTCT
CGCTCTTCATCGACGCCGACTTCGCGCAACTCGACGCCGCGCACGCCGCGGGTGCGCCGGTCGTCGAAATCCACACCGGG
CATTACGCCGATGCGGCGAGCGATGCCGCGCGTGCCGCCGAATTCGAACGCATCCGCCGGGCGGTCGCCTACGGCCGCAG
CCTCGGCCTCACCGTCAACGCCGGCCACGGCCTGACCTATCACAACGTGCAGCCCATCGCCGCCTTGCCCGGCATCCATG
AGCTCAACATCGGCCACGCGATCGTCGCGCAGGCGCTGTTCGTCGGCTGGAAGGAAGCGGTCGCGGAAATGAAGCGGCTG
ATGGTCGCCGCGGCGGGGTAG

Upstream 100 bases:

>100_bases
CTGAATCCATGATGCCCTCGCCCTTGTCCCACACGCTTTCCGGGCCTCACCGCATTGCGCCGACCTTCGTTTCGCGCGTC
TCGCGTCGCGCACGGCTCGC

Downstream 100 bases:

>100_bases
GGCGAGCTCCAAATCGCGGCGCGCCGCGGGTCAGCGATGCCGCCGGCGCGCCATCAGGCCGACGAGGCCGAGGCCTGCGG
CCATCAGCGCGTAGGACTCG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MGGAMSIYLGVNIDHIATLRQARKTRYPSPVEAALAAETAGADSITLHLREDRRHIQDADVTILRQTLKTKMNLEMAVTE
EMLGIALETRPSDVCLVPEKREELTTEGGLDVAGQQAKMRDACARLGDAGIRVSLFIDADFAQLDAAHAAGAPVVEIHTG
HYADAASDAARAAEFERIRRAVAYGRSLGLTVNAGHGLTYHNVQPIAALPGIHELNIGHAIVAQALFVGWKEAVAEMKRL
MVAAAG

Sequences:

>Translated_246_residues
MGGAMSIYLGVNIDHIATLRQARKTRYPSPVEAALAAETAGADSITLHLREDRRHIQDADVTILRQTLKTKMNLEMAVTE
EMLGIALETRPSDVCLVPEKREELTTEGGLDVAGQQAKMRDACARLGDAGIRVSLFIDADFAQLDAAHAAGAPVVEIHTG
HYADAASDAARAAEFERIRRAVAYGRSLGLTVNAGHGLTYHNVQPIAALPGIHELNIGHAIVAQALFVGWKEAVAEMKRL
MVAAAG
>Mature_245_residues
GGAMSIYLGVNIDHIATLRQARKTRYPSPVEAALAAETAGADSITLHLREDRRHIQDADVTILRQTLKTKMNLEMAVTEE
MLGIALETRPSDVCLVPEKREELTTEGGLDVAGQQAKMRDACARLGDAGIRVSLFIDADFAQLDAAHAAGAPVVEIHTGH
YADAASDAARAAEFERIRRAVAYGRSLGLTVNAGHGLTYHNVQPIAALPGIHELNIGHAIVAQALFVGWKEAVAEMKRLM
VAAAG

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=241, Percent_Identity=70.1244813278008, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_THIDA (Q3SH53)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315841.1
- ProteinModelPortal:   Q3SH53
- SMR:   Q3SH53
- STRING:   Q3SH53
- GeneID:   3672255
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2083
- NMPDR:   fig|292415.3.peg.2238
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q3SH53
- ProtClustDB:   PRK05265
- BioCyc:   TDEN292415:TBD_2083-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26305; Mature: 26174

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 196-196 BINDING 12-12 BINDING 23-23 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGAMSIYLGVNIDHIATLRQARKTRYPSPVEAALAAETAGADSITLHLREDRRHIQDAD
CCCCEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCH
VTILRQTLKTKMNLEMAVTEEMLGIALETRPSDVCLVPEKREELTTEGGLDVAGQQAKMR
HHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHHH
DACARLGDAGIRVSLFIDADFAQLDAAHAAGAPVVEIHTGHYADAASDAARAAEFERIRR
HHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
AVAYGRSLGLTVNAGHGLTYHNVQPIAALPGIHELNIGHAIVAQALFVGWKEAVAEMKRL
HHHHCCCCCEEEECCCCEEECCCCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
MVAAAG
HHHCCC
>Mature Secondary Structure 
GGAMSIYLGVNIDHIATLRQARKTRYPSPVEAALAAETAGADSITLHLREDRRHIQDAD
CCCEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCH
VTILRQTLKTKMNLEMAVTEEMLGIALETRPSDVCLVPEKREELTTEGGLDVAGQQAKMR
HHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHHH
DACARLGDAGIRVSLFIDADFAQLDAAHAAGAPVVEIHTGHYADAASDAARAAEFERIRR
HHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
AVAYGRSLGLTVNAGHGLTYHNVQPIAALPGIHELNIGHAIVAQALFVGWKEAVAEMKRL
HHHHCCCCCEEEECCCCEEECCCCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
MVAAAG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA