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The map label for this gene is glgB

Identifier: 74318076

GI number: 74318076

Start: 2146690

End: 2148912

Strand: Reverse

Name: glgB

Synonym: Tbd_2058

Alternate gene names: 74318076

Gene position: 2148912-2146690 (Counterclockwise)

Preceding gene: 74318077

Following gene: 74318075

Centisome position: 73.85

GC content: 67.57

Gene sequence:

>2223_bases
ATGCGCAAGCTAGTCGAGGCCACGCAGGCCGGCAGTGAAGCGTCCGTGGCGCTCGCGCCGAGCGCGCCGATCCAGCGTCT
GCAGGCCGGCCTGCACCACGATCCCTTCGAGGTGCTCGGCGTGCACGTCCAGCCCGACGGCAGCAAGCTCGTCCGCGCCT
TCCTGCCCGCCGCCGAGGCGGTCGAAGTCGCCGGCGTGCGCATGACGCGCGTGCCCGGCACCGATTGTTTCGAGCGTCTA
TTGCCGCCCGGGACGGCGCTCGAAGCGCATCCGCTGCTGACCTGGCAGGACAAGCGCAGCGGCGCCTGGCAGCGCCTGCG
CTCGCCCTACAGCTTCGCGCCGCAACTCGGCGAGATGGATTTGTATCTGTTCGGCGAGGGTCGCCATTTCGAGATCTGGA
AGGTGCTCGGTGCGCGCGTGAAGACCGTCGACGGCGTCACCGGCTGTCTGTTCGCGGTGTGGGCGCCGGCCGTGCTGCGC
GTCAGCGTCGTCGGCGACTTCAACGACTGGGACGGCCGGCGCCATCCGATGCGCTGTCGCGGCGTCTCGGGGGTCTGGGA
GCTGTTCATCCCCGGGCTGGAGGCGGGGCACGCCTACAAATACGAAATCCTCGGCCGCCACGGCGAGCGCGTGACCAAGA
CCGATCCCTACGCGCGGCAGATGTTCCTGCGCCCCGAAACGACCAGCCGCGTGCCCGACGAGCGCCCCTACGCCTGGGGC
GACGCCGAGTGGCTTGCCGCGCGCACGCGCTTCGACTGGCAGCACCGGCCGATGAGCGTCTACGAAGTCCATCCCGGTTC
GTGGCGCCGCCGCGCCGACGGCAGCTTCTACAGCTGGCGCGAACTCACGGCCGAGCTGATTCCCTATGTGCGCGATCTGG
GCTACACCCACATCGAATTGCTGCCGGTCGCCGAGCATCCGTTCGACGCCTCGTGGGGCTACCAGGTCTCGGGTTACTAC
GCAGCGACGGCGCGCTTCGGCTCGCCCGACGACCTGCGCGCCTTCGTCGACGCGTGCCACCAGGCCGGGCTCGGCGTGCT
GCTCGACTGGGTGCCGGGGCATTTCCCCAAGGACGATTTCGCGCTCGCCCGCTTCACCGGCGAGCCGCTCTATGAACATG
CCGACCCGCGCCGCGGCGAGCATCAGGACTGGGGCACGCTGGTCTTCGATTTCGGCCGCAATGAGGTGCGCAATTTCCTC
GTCGCCAATGCGCTCTACTGGCTCGAGGAGTTTCACATCGACGGTCTCAGGGTCGATGCGGTCGCGTCGATGCTCTACCT
CGACTATTCGCGGCGCCACGGCGAATGGCTGCCGAACCAGCACGGCGGACGCGAGAACCTCGAGGCGATCCATTTCCTGC
ACGAGGTCAACGCCGAGGTCCACGCGCGTTTTCCGGGGGCGATCACGATCGCTGAGGAGTCGACGGCGTGGCCGGCGGTG
TCGCGGCCGATCGAGCTCGGCGGCCTCGGCTTCTCGATGAAATGGAACATGGGGTGGATGAACGACACGCTCGACTACAT
CGAGAAGGAGCCGGTCTACCGCAAGTATCAGCATAACCAGCTGACCTTCAGCCAGATGTACGCGTGGAGCGAGAATTTCG
TGCTGCCGCTGTCGCACGACGAGGTCGTGCACCTGAAGAAAAGCCTGCTCGACAAGATGCCGGGCGACCGCTGGCAGCGC
TTCGCCAATCTGCGTCTGCTCTACGCCTGGCAGTATGCGCATCCGGGCAAGAAGCTGCTCTTCATGGGCGGCGAATTCGG
CCAGTGGAACGAGTGGCGCGAAGCGGGACAGCTCGACTGGGTCCTGCTCGGCTTTCCCGAGCACGACGGCATCCGCGCGC
TGCTGCGCGACCTCAACCGGCTCTACCGCGACGAGGCGGCGCTGCACTTCTGGGATTTCGACCCGCGCGGCTTCCGCTGG
ATCGACTGTCACGACGCCGACCAGTCGGTGCTGAGCCTCGTCCGCGAGGGACCCGACGGCGCGCCGCCGATCGTCGTCCT
GCTCAATTTCACGCCGGTGCCGCGGCACGGCTATCGCATCGGCGTGCCCCGCGCGGGCGCGTGGTGCGAGGTGCTGAACA
GCGATTCGATGTACTACGGCGGCAGCAACCTCGGCAACGGCAAGCCCTTGTATCCCGCCGCCGTGCCTTGGATGGGCTTC
GGGCAGTCGATCGAAGTGACGCTGCCGCCGCTCGGGGCGATTTTCCTCAAGCCCTGTCCCTGA

Upstream 100 bases:

>100_bases
CGGGCCGCGAGCAGGGCAACTGGACCTGGCGCTTCGGCTGGGACGCGCTGCCCGACGGTCTCGCCGCGCGCCTGCGCGAA
CAACTCCAGGCGGTCGATCG

Downstream 100 bases:

>100_bases
AGGGGACGCAGATGGATTCGACAAGAACGACGGGAAATAACAATGAAACGCGGAGCAATGAAGGTGCTGTTCGTCGCGAG
CGAGGCCTTTCCCCTGGTCA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MRKLVEATQAGSEASVALAPSAPIQRLQAGLHHDPFEVLGVHVQPDGSKLVRAFLPAAEAVEVAGVRMTRVPGTDCFERL
LPPGTALEAHPLLTWQDKRSGAWQRLRSPYSFAPQLGEMDLYLFGEGRHFEIWKVLGARVKTVDGVTGCLFAVWAPAVLR
VSVVGDFNDWDGRRHPMRCRGVSGVWELFIPGLEAGHAYKYEILGRHGERVTKTDPYARQMFLRPETTSRVPDERPYAWG
DAEWLAARTRFDWQHRPMSVYEVHPGSWRRRADGSFYSWRELTAELIPYVRDLGYTHIELLPVAEHPFDASWGYQVSGYY
AATARFGSPDDLRAFVDACHQAGLGVLLDWVPGHFPKDDFALARFTGEPLYEHADPRRGEHQDWGTLVFDFGRNEVRNFL
VANALYWLEEFHIDGLRVDAVASMLYLDYSRRHGEWLPNQHGGRENLEAIHFLHEVNAEVHARFPGAITIAEESTAWPAV
SRPIELGGLGFSMKWNMGWMNDTLDYIEKEPVYRKYQHNQLTFSQMYAWSENFVLPLSHDEVVHLKKSLLDKMPGDRWQR
FANLRLLYAWQYAHPGKKLLFMGGEFGQWNEWREAGQLDWVLLGFPEHDGIRALLRDLNRLYRDEAALHFWDFDPRGFRW
IDCHDADQSVLSLVREGPDGAPPIVVLLNFTPVPRHGYRIGVPRAGAWCEVLNSDSMYYGGSNLGNGKPLYPAAVPWMGF
GQSIEVTLPPLGAIFLKPCP

Sequences:

>Translated_740_residues
MRKLVEATQAGSEASVALAPSAPIQRLQAGLHHDPFEVLGVHVQPDGSKLVRAFLPAAEAVEVAGVRMTRVPGTDCFERL
LPPGTALEAHPLLTWQDKRSGAWQRLRSPYSFAPQLGEMDLYLFGEGRHFEIWKVLGARVKTVDGVTGCLFAVWAPAVLR
VSVVGDFNDWDGRRHPMRCRGVSGVWELFIPGLEAGHAYKYEILGRHGERVTKTDPYARQMFLRPETTSRVPDERPYAWG
DAEWLAARTRFDWQHRPMSVYEVHPGSWRRRADGSFYSWRELTAELIPYVRDLGYTHIELLPVAEHPFDASWGYQVSGYY
AATARFGSPDDLRAFVDACHQAGLGVLLDWVPGHFPKDDFALARFTGEPLYEHADPRRGEHQDWGTLVFDFGRNEVRNFL
VANALYWLEEFHIDGLRVDAVASMLYLDYSRRHGEWLPNQHGGRENLEAIHFLHEVNAEVHARFPGAITIAEESTAWPAV
SRPIELGGLGFSMKWNMGWMNDTLDYIEKEPVYRKYQHNQLTFSQMYAWSENFVLPLSHDEVVHLKKSLLDKMPGDRWQR
FANLRLLYAWQYAHPGKKLLFMGGEFGQWNEWREAGQLDWVLLGFPEHDGIRALLRDLNRLYRDEAALHFWDFDPRGFRW
IDCHDADQSVLSLVREGPDGAPPIVVLLNFTPVPRHGYRIGVPRAGAWCEVLNSDSMYYGGSNLGNGKPLYPAAVPWMGF
GQSIEVTLPPLGAIFLKPCP
>Mature_740_residues
MRKLVEATQAGSEASVALAPSAPIQRLQAGLHHDPFEVLGVHVQPDGSKLVRAFLPAAEAVEVAGVRMTRVPGTDCFERL
LPPGTALEAHPLLTWQDKRSGAWQRLRSPYSFAPQLGEMDLYLFGEGRHFEIWKVLGARVKTVDGVTGCLFAVWAPAVLR
VSVVGDFNDWDGRRHPMRCRGVSGVWELFIPGLEAGHAYKYEILGRHGERVTKTDPYARQMFLRPETTSRVPDERPYAWG
DAEWLAARTRFDWQHRPMSVYEVHPGSWRRRADGSFYSWRELTAELIPYVRDLGYTHIELLPVAEHPFDASWGYQVSGYY
AATARFGSPDDLRAFVDACHQAGLGVLLDWVPGHFPKDDFALARFTGEPLYEHADPRRGEHQDWGTLVFDFGRNEVRNFL
VANALYWLEEFHIDGLRVDAVASMLYLDYSRRHGEWLPNQHGGRENLEAIHFLHEVNAEVHARFPGAITIAEESTAWPAV
SRPIELGGLGFSMKWNMGWMNDTLDYIEKEPVYRKYQHNQLTFSQMYAWSENFVLPLSHDEVVHLKKSLLDKMPGDRWQR
FANLRLLYAWQYAHPGKKLLFMGGEFGQWNEWREAGQLDWVLLGFPEHDGIRALLRDLNRLYRDEAALHFWDFDPRGFRW
IDCHDADQSVLSLVREGPDGAPPIVVLLNFTPVPRHGYRIGVPRAGAWCEVLNSDSMYYGGSNLGNGKPLYPAAVPWMGF
GQSIEVTLPPLGAIFLKPCP

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=653, Percent_Identity=28.0245022970904, Blast_Score=190, Evalue=4e-48,
Organism=Escherichia coli, GI1789839, Length=724, Percent_Identity=53.4530386740332, Blast_Score=742, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=647, Percent_Identity=26.5842349304482, Blast_Score=190, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI32564391, Length=612, Percent_Identity=25.9803921568627, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320826, Length=648, Percent_Identity=25.462962962963, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI28573410, Length=629, Percent_Identity=27.8219395866455, Blast_Score=198, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_THIDA (Q3SH78)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315816.1
- ProteinModelPortal:   Q3SH78
- SMR:   Q3SH78
- STRING:   Q3SH78
- GeneID:   3672229
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2058
- NMPDR:   fig|292415.3.peg.2214
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   Q3SH78
- ProtClustDB:   PRK05402
- BioCyc:   TDEN292415:TBD_2058-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 84263; Mature: 84263

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: ACT_SITE 314-314 ACT_SITE 349-349 ACT_SITE 354-354 ACT_SITE 417-417 ACT_SITE 419-419 ACT_SITE 472-472 ACT_SITE 539-539 ACT_SITE 540-540

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLVEATQAGSEASVALAPSAPIQRLQAGLHHDPFEVLGVHVQPDGSKLVRAFLPAAEA
CCHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCHHEEEEECCCHHHHHHHHCCHHHH
VEVAGVRMTRVPGTDCFERLLPPGTALEAHPLLTWQDKRSGAWQRLRSPYSFAPQLGEMD
HHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCEE
LYLFGEGRHFEIWKVLGARVKTVDGVTGCLFAVWAPAVLRVSVVGDFNDWDGRRHPMRCR
EEEEECCCCCHHHHHHCCHHEECCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEEC
GVSGVWELFIPGLEAGHAYKYEILGRHGERVTKTDPYARQMFLRPETTSRVPDERPYAWG
CCCHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCC
DAEWLAARTRFDWQHRPMSVYEVHPGSWRRRADGSFYSWRELTAELIPYVRDLGYTHIEL
CHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
LPVAEHPFDASWGYQVSGYYAATARFGSPDDLRAFVDACHQAGLGVLLDWVPGHFPKDDF
EECCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
ALARFTGEPLYEHADPRRGEHQDWGTLVFDFGRNEVRNFLVANALYWLEEFHIDGLRVDA
EEEEECCCCHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHH
VASMLYLDYSRRHGEWLPNQHGGRENLEAIHFLHEVNAEVHARFPGAITIAEESTAWPAV
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCC
SRPIELGGLGFSMKWNMGWMNDTLDYIEKEPVYRKYQHNQLTFSQMYAWSENFVLPLSHD
CCCEEECCCEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHCCCCCEEECCCC
EVVHLKKSLLDKMPGDRWQRFANLRLLYAWQYAHPGKKLLFMGGEFGQWNEWREAGQLDW
HHHHHHHHHHHHCCCHHHHHHHCEEEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCE
VLLGFPEHDGIRALLRDLNRLYRDEAALHFWDFDPRGFRWIDCHDADQSVLSLVREGPDG
EEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCC
APPIVVLLNFTPVPRHGYRIGVPRAGAWCEVLNSDSMYYGGSNLGNGKPLYPAAVPWMGF
CCCEEEEEECCCCCCCCEEECCCCCCCHHHEECCCCEEECCCCCCCCCCCCCCCCCCCCC
GQSIEVTLPPLGAIFLKPCP
CCEEEEEECCCCEEEEECCC
>Mature Secondary Structure
MRKLVEATQAGSEASVALAPSAPIQRLQAGLHHDPFEVLGVHVQPDGSKLVRAFLPAAEA
CCHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCHHEEEEECCCHHHHHHHHCCHHHH
VEVAGVRMTRVPGTDCFERLLPPGTALEAHPLLTWQDKRSGAWQRLRSPYSFAPQLGEMD
HHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHCCCCCCCCCCCCEE
LYLFGEGRHFEIWKVLGARVKTVDGVTGCLFAVWAPAVLRVSVVGDFNDWDGRRHPMRCR
EEEEECCCCCHHHHHHCCHHEECCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEEC
GVSGVWELFIPGLEAGHAYKYEILGRHGERVTKTDPYARQMFLRPETTSRVPDERPYAWG
CCCHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCC
DAEWLAARTRFDWQHRPMSVYEVHPGSWRRRADGSFYSWRELTAELIPYVRDLGYTHIEL
CHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
LPVAEHPFDASWGYQVSGYYAATARFGSPDDLRAFVDACHQAGLGVLLDWVPGHFPKDDF
EECCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
ALARFTGEPLYEHADPRRGEHQDWGTLVFDFGRNEVRNFLVANALYWLEEFHIDGLRVDA
EEEEECCCCHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHH
VASMLYLDYSRRHGEWLPNQHGGRENLEAIHFLHEVNAEVHARFPGAITIAEESTAWPAV
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCC
SRPIELGGLGFSMKWNMGWMNDTLDYIEKEPVYRKYQHNQLTFSQMYAWSENFVLPLSHD
CCCEEECCCEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHCCCCCEEECCCC
EVVHLKKSLLDKMPGDRWQRFANLRLLYAWQYAHPGKKLLFMGGEFGQWNEWREAGQLDW
HHHHHHHHHHHHCCCHHHHHHHCEEEEEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCE
VLLGFPEHDGIRALLRDLNRLYRDEAALHFWDFDPRGFRWIDCHDADQSVLSLVREGPDG
EEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCC
APPIVVLLNFTPVPRHGYRIGVPRAGAWCEVLNSDSMYYGGSNLGNGKPLYPAAVPWMGF
CCCEEEEEECCCCCCCCEEECCCCCCCHHHEECCCCEEECCCCCCCCCCCCCCCCCCCCC
GQSIEVTLPPLGAIFLKPCP
CCEEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA