Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 74318009

GI number: 74318009

Start: 2081145

End: 2082986

Strand: Reverse

Name: ilvI [H]

Synonym: Tbd_1991

Alternate gene names: 74318009

Gene position: 2082986-2081145 (Counterclockwise)

Preceding gene: 74318010

Following gene: 74318008

Centisome position: 71.58

GC content: 62.43

Gene sequence:

>1842_bases
TTGCCGTCGCCAGCATTTTTTTCCATTCCCACAGCTGGAAACGCGCCACGCGAAGGCGTTTTCTTGACCGAAACGCGGCA
AAACGATTACCCTCACGGGTTCACCAACGAATTGATTTTTTGCCAGTTTATGGAAGCCACGGGCGCCGAGATCGTCGTAC
GTTGTCTGGCTGAAGAGGGGGTCGAGTTCGTCTTCGGCTATCCCGGCGGAGCCGTGCTCAACATCTACGACGAGATCTTC
AAGCAGAACAGGTTCAAGCACGTGCTGGTGCGCCACGAACAGGCGGCCGTCCACGCTGCCGACGCCTATGCGCGCTCGAC
CGGCCGCGTCGGCGTGGCGCTCGTCACCTCGGGGCCCGGCGTCACCAACGCTGTGACCGGCATCGCCACGGCGTATATGG
ATTCGGTGCCGATCGTCGTCGTCACCGGCCAGGTGCCGACGCCCGCGATCGGGCTCGACGCCTTCCAGGAAGTCGACACC
GTCGGCATCACACGGCCCTGCGTCAAACACAATTTCCTCGTCAAGGACGTCAAGGATCTGGCCGCGACGATGAAGAAGGC
CTTCATCATCGCAGCCACCGGTCGCCCCGGCCCGGTGGTCGTCGACGTACCCAAGGACGTGACCTACCACGTGACCGAGT
TCGAATATCCGGCCACGGTCGAGATGCGTTCGTACAACCCCGTTGTCAAAGGCCACAGCGGTCAGTTGAAGCGCGCCGTG
CAGATGCTGCTCGAAGCCAAGCGCCCGATGATCTACGCGGGCGGCGGCGTCGTCCTCGGCGACGCGTCGGCGCAGCTGAC
CGAACTCGCGCGCCTGCTCGGCTTTCCCTGCACCAATACGCTGATGGGTCTCGGCGGTTATCCTGCGACCGACAGGCAGA
ACCTCGGCATGCTCGGCATGCACGGCACCTATGAAGCGAACATGGCGATGCAGCACTGCGACGTGCTGCTGGCCGTCGGC
GCGCGCTTCGACGACCGCGTGATCGGCAACCCCGCGCACTTCGCCAAGGAGCAGCGTCGCATCATCCACATCGACATCGA
CCCGTCTTCGATCTCGAAGCGCGTCAAGGTCGACGTGCCCATCGTCGGCGACGTCAGGAACGTGCTGCGCGACATGCTGA
GCCTGCTGAAGCAGGCCAAGGAAGTGCCCGACGCTGCGGCGCTCAACGCGTGGTGGACGCAGATCGAATTGTGGCGCTCG
AAGAACTGCCTCAAGTACAACCGCAACAGCCCGATCATCAAGCCGCAGTATGTGGTCGAAAAGCTGTGGGAAGTCACCAA
GGGCGACGCCTTCATCACCTCCGACGTCGGCCAGCACCAGATGTGGGCGGCCCAGTACTACAAGTTCGACAAGCCGCGCC
GCTGGATCAATTCCGGTGGCCTCGGCACCATGGGCGTCGGCCTGCCGTATGCAATGGGCGTGCAACTCGCGCATCCGGAG
GCGCAGGTCGCCTGCATCACCGGCGAGTCGTCGATCCAGATGAACATCCAGGAGCTTTCGACCTGCAAGCAGTACCGCAT
TCCGGTCAAGGTCGTCACGCTCAATAACCGCTACATGGGCATGGTGAGGCAGTGGCAGGAGTTCTTCTACGGCAGCCGCT
ACGCCGAGTCGTACATGGAGTCGCTGCCCGACTTCGTCAAGCTCGCCGAAGCCTACGGCCACGTCGGCATGCGCATCGAC
AAGCCCGAGGACGTCGAAGCGGCGCTCAAGGAAGCCTTCTCGCCTGTACTCAAGGAAAGAATGGTGTTCATGGACTTCCA
GACCGACCAGACCGAGAACGTCTTCCCGATGGTCGAAGGCGGCAAGGGCCTGTCGGAAATGATTCTGGCGGAGGAGCTGT
GA

Upstream 100 bases:

>100_bases
AACGCCGACGCGTCGTTGAACCAGATCATGCAGGCGCTGGCCGAAGCGGCCCACGAGTAGCCGCCTGCGTCCCCGGCAAA
AAGCCCGCCGACCGGGTCTT

Downstream 100 bases:

>100_bases
TGAGCACCCGCCATATCATTTCGCTCTTGATGGAGAACGAGGCGGGTGCGCTGTCGCGCGTCGCCGGTCTCTTCTCCGCG
CGCGCCTACAACATCGAATC

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MPSPAFFSIPTAGNAPREGVFLTETRQNDYPHGFTNELIFCQFMEATGAEIVVRCLAEEGVEFVFGYPGGAVLNIYDEIF
KQNRFKHVLVRHEQAAVHAADAYARSTGRVGVALVTSGPGVTNAVTGIATAYMDSVPIVVVTGQVPTPAIGLDAFQEVDT
VGITRPCVKHNFLVKDVKDLAATMKKAFIIAATGRPGPVVVDVPKDVTYHVTEFEYPATVEMRSYNPVVKGHSGQLKRAV
QMLLEAKRPMIYAGGGVVLGDASAQLTELARLLGFPCTNTLMGLGGYPATDRQNLGMLGMHGTYEANMAMQHCDVLLAVG
ARFDDRVIGNPAHFAKEQRRIIHIDIDPSSISKRVKVDVPIVGDVRNVLRDMLSLLKQAKEVPDAAALNAWWTQIELWRS
KNCLKYNRNSPIIKPQYVVEKLWEVTKGDAFITSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGVQLAHPE
AQVACITGESSIQMNIQELSTCKQYRIPVKVVTLNNRYMGMVRQWQEFFYGSRYAESYMESLPDFVKLAEAYGHVGMRID
KPEDVEAALKEAFSPVLKERMVFMDFQTDQTENVFPMVEGGKGLSEMILAEEL

Sequences:

>Translated_613_residues
MPSPAFFSIPTAGNAPREGVFLTETRQNDYPHGFTNELIFCQFMEATGAEIVVRCLAEEGVEFVFGYPGGAVLNIYDEIF
KQNRFKHVLVRHEQAAVHAADAYARSTGRVGVALVTSGPGVTNAVTGIATAYMDSVPIVVVTGQVPTPAIGLDAFQEVDT
VGITRPCVKHNFLVKDVKDLAATMKKAFIIAATGRPGPVVVDVPKDVTYHVTEFEYPATVEMRSYNPVVKGHSGQLKRAV
QMLLEAKRPMIYAGGGVVLGDASAQLTELARLLGFPCTNTLMGLGGYPATDRQNLGMLGMHGTYEANMAMQHCDVLLAVG
ARFDDRVIGNPAHFAKEQRRIIHIDIDPSSISKRVKVDVPIVGDVRNVLRDMLSLLKQAKEVPDAAALNAWWTQIELWRS
KNCLKYNRNSPIIKPQYVVEKLWEVTKGDAFITSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGVQLAHPE
AQVACITGESSIQMNIQELSTCKQYRIPVKVVTLNNRYMGMVRQWQEFFYGSRYAESYMESLPDFVKLAEAYGHVGMRID
KPEDVEAALKEAFSPVLKERMVFMDFQTDQTENVFPMVEGGKGLSEMILAEEL
>Mature_612_residues
PSPAFFSIPTAGNAPREGVFLTETRQNDYPHGFTNELIFCQFMEATGAEIVVRCLAEEGVEFVFGYPGGAVLNIYDEIFK
QNRFKHVLVRHEQAAVHAADAYARSTGRVGVALVTSGPGVTNAVTGIATAYMDSVPIVVVTGQVPTPAIGLDAFQEVDTV
GITRPCVKHNFLVKDVKDLAATMKKAFIIAATGRPGPVVVDVPKDVTYHVTEFEYPATVEMRSYNPVVKGHSGQLKRAVQ
MLLEAKRPMIYAGGGVVLGDASAQLTELARLLGFPCTNTLMGLGGYPATDRQNLGMLGMHGTYEANMAMQHCDVLLAVGA
RFDDRVIGNPAHFAKEQRRIIHIDIDPSSISKRVKVDVPIVGDVRNVLRDMLSLLKQAKEVPDAAALNAWWTQIELWRSK
NCLKYNRNSPIIKPQYVVEKLWEVTKGDAFITSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGVQLAHPEA
QVACITGESSIQMNIQELSTCKQYRIPVKVVTLNNRYMGMVRQWQEFFYGSRYAESYMESLPDFVKLAEAYGHVGMRIDK
PEDVEAALKEAFSPVLKERMVFMDFQTDQTENVFPMVEGGKGLSEMILAEEL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=561, Percent_Identity=26.9162210338681, Blast_Score=168, Evalue=2e-41,
Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=25.2688172043011, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI87081685, Length=567, Percent_Identity=59.6119929453263, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=39.9293286219081, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=540, Percent_Identity=33.1481481481482, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=28.256880733945, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788716, Length=485, Percent_Identity=25.360824742268, Blast_Score=146, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=578, Percent_Identity=25.4325259515571, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=543, Percent_Identity=26.3351749539595, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=543, Percent_Identity=26.3351749539595, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=43.6332767402377, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320816, Length=501, Percent_Identity=25.748502994012, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=498, Percent_Identity=23.6947791164659, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321524, Length=501, Percent_Identity=22.9540918163673, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=24.0480961923848, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=27.3100616016427, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 67805; Mature: 67674

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPAFFSIPTAGNAPREGVFLTETRQNDYPHGFTNELIFCQFMEATGAEIVVRCLAEEG
CCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHCC
VEFVFGYPGGAVLNIYDEIFKQNRFKHVLVRHEQAAVHAADAYARSTGRVGVALVTSGPG
CEEEEECCCCHHHHHHHHHHHCCCCHHHHEECHHHHHHHHHHHHHCCCCEEEEEEECCCC
VTNAVTGIATAYMDSVPIVVVTGQVPTPAIGLDAFQEVDTVGITRPCVKHNFLVKDVKDL
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHH
AATMKKAFIIAATGRPGPVVVDVPKDVTYHVTEFEYPATVEMRSYNPVVKGHSGQLKRAV
HHHHHCEEEEEECCCCCCEEEECCCCCEEEEEEECCCCEEEEECCCCEEECCCHHHHHHH
QMLLEAKRPMIYAGGGVVLGDASAQLTELARLLGFPCTNTLMGLGGYPATDRQNLGMLGM
HHHHHCCCCEEEECCEEEEECCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEEC
HGTYEANMAMQHCDVLLAVGARFDDRVIGNPAHFAKEQRRIIHIDIDPSSISKRVKVDVP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEEECHHHHCCEEEEECC
IVGDVRNVLRDMLSLLKQAKEVPDAAALNAWWTQIELWRSKNCLKYNRNSPIIKPQYVVE
EEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHH
KLWEVTKGDAFITSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGVQLAHPE
HHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCHHHHCCCCCCEECCCCHHHHCEEEECCC
AQVACITGESSIQMNIQELSTCKQYRIPVKVVTLNNRYMGMVRQWQEFFYGSRYAESYME
CEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHH
SLPDFVKLAEAYGHVGMRIDKPEDVEAALKEAFSPVLKERMVFMDFQTDQTENVFPMVEG
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEC
GKGLSEMILAEEL
CCCHHHHHHHHCC
>Mature Secondary Structure 
PSPAFFSIPTAGNAPREGVFLTETRQNDYPHGFTNELIFCQFMEATGAEIVVRCLAEEG
CCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHCC
VEFVFGYPGGAVLNIYDEIFKQNRFKHVLVRHEQAAVHAADAYARSTGRVGVALVTSGPG
CEEEEECCCCHHHHHHHHHHHCCCCHHHHEECHHHHHHHHHHHHHCCCCEEEEEEECCCC
VTNAVTGIATAYMDSVPIVVVTGQVPTPAIGLDAFQEVDTVGITRPCVKHNFLVKDVKDL
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHH
AATMKKAFIIAATGRPGPVVVDVPKDVTYHVTEFEYPATVEMRSYNPVVKGHSGQLKRAV
HHHHHCEEEEEECCCCCCEEEECCCCCEEEEEEECCCCEEEEECCCCEEECCCHHHHHHH
QMLLEAKRPMIYAGGGVVLGDASAQLTELARLLGFPCTNTLMGLGGYPATDRQNLGMLGM
HHHHHCCCCEEEECCEEEEECCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEEC
HGTYEANMAMQHCDVLLAVGARFDDRVIGNPAHFAKEQRRIIHIDIDPSSISKRVKVDVP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEEECHHHHCCEEEEECC
IVGDVRNVLRDMLSLLKQAKEVPDAAALNAWWTQIELWRSKNCLKYNRNSPIIKPQYVVE
EEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHH
KLWEVTKGDAFITSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGVQLAHPE
HHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCHHHHCCCCCCEECCCCHHHHCEEEECCC
AQVACITGESSIQMNIQELSTCKQYRIPVKVVTLNNRYMGMVRQWQEFFYGSRYAESYME
CEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHH
SLPDFVKLAEAYGHVGMRIDKPEDVEAALKEAFSPVLKERMVFMDFQTDQTENVFPMVEG
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEC
GKGLSEMILAEEL
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]