Click here to switch to the map view.

The map label for this gene is truA

Identifier: 74317934

GI number: 74317934

Start: 2011555

End: 2012337

Strand: Reverse

Name: truA

Synonym: Tbd_1916

Alternate gene names: 74317934

Gene position: 2012337-2011555 (Counterclockwise)

Preceding gene: 74317935

Following gene: 74317933

Centisome position: 69.16

GC content: 68.97

Gene sequence:

>783_bases
ATGATGCGGATCGCGGTGGGTCTCGAATATCGTGGCGTCGGTTTCTGCGGCTGGCAGTCGCAGCCGCAAGCCTGCGGCGT
CCAGGACGCCGTGGAAAAAGCCGTGTCGGCGATCGCCGGCGAGGCGATCACGGTCACCGCCGCCGGCCGCACCGACACCG
GCGTGCATGCGGCCTTGCAGATCGTGCACTTCGACACGACCTCGGTCCGCCCGCTGACCGCGTGGGTGCGCGGCGTCAAC
AGCCATCTTCCCGCGGGCGTCGCCGTGTTGTGGGCGCGCGAAGTCGATGCGGAATTCCATGCACGGTTTGCCGCTTTCGA
GCGCGGCTATCGCTACGTGCTGCTCAACCATCCGGTGCGCCCCGGCCTCAACGCCGGGCTCGTCGGCTGGCATCACCGGC
CGCTCGACGTGGCGTGCATGAATCGGGCGGCGTCGCCGTTGATCGGACGGCACGACTTTTCCGCGTTCCGCGCAGCCGAG
TGCCAGGCCCGCTCGCCGGTCAAGGAGTTGCGCCGCGCGCTGATCGAGCGGCGCGGCGATTACCTGCTGTGCGATTTCCG
CGCGGACGGTTTTCTGCACCACATGGTGCGAAACCTCATGGGCTGTCTGGTCCAGATCGGTGCCGGCGGCCGCCCGCCCG
AGTGGCTGCACGAGGTGCTGGCCGGGCGCGACCGCACGCGTGCCGCGCCGACCTTCGAGGCGGCAGGACTGTACCTCACG
CACATCCGGTATCCTGCGCGCTTTGCCCTGCCGGAAAGCTCCGAACGATGGCCGTTCGCGTAA

Upstream 100 bases:

>100_bases
ACCTACGCGCTTGCGTGCGAACGCTGGCAAAGGCGCGACAGCGCCGTCATACTCGCGGGCGTGTTGCTCGTGGGGATCGT
GCTGGCCTGAGGCCGACTGG

Downstream 100 bases:

>100_bases
AAATCTGCGGAATTACCCGCGTCGACGACCTGCACGCTGCGTGCGACGCCGGCGCAGATGCGCTTGGCTTCGTTTTCTAC
GAGAAGAGCCCGCGTCATCT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MMRIAVGLEYRGVGFCGWQSQPQACGVQDAVEKAVSAIAGEAITVTAAGRTDTGVHAALQIVHFDTTSVRPLTAWVRGVN
SHLPAGVAVLWAREVDAEFHARFAAFERGYRYVLLNHPVRPGLNAGLVGWHHRPLDVACMNRAASPLIGRHDFSAFRAAE
CQARSPVKELRRALIERRGDYLLCDFRADGFLHHMVRNLMGCLVQIGAGGRPPEWLHEVLAGRDRTRAAPTFEAAGLYLT
HIRYPARFALPESSERWPFA

Sequences:

>Translated_260_residues
MMRIAVGLEYRGVGFCGWQSQPQACGVQDAVEKAVSAIAGEAITVTAAGRTDTGVHAALQIVHFDTTSVRPLTAWVRGVN
SHLPAGVAVLWAREVDAEFHARFAAFERGYRYVLLNHPVRPGLNAGLVGWHHRPLDVACMNRAASPLIGRHDFSAFRAAE
CQARSPVKELRRALIERRGDYLLCDFRADGFLHHMVRNLMGCLVQIGAGGRPPEWLHEVLAGRDRTRAAPTFEAAGLYLT
HIRYPARFALPESSERWPFA
>Mature_260_residues
MMRIAVGLEYRGVGFCGWQSQPQACGVQDAVEKAVSAIAGEAITVTAAGRTDTGVHAALQIVHFDTTSVRPLTAWVRGVN
SHLPAGVAVLWAREVDAEFHARFAAFERGYRYVLLNHPVRPGLNAGLVGWHHRPLDVACMNRAASPLIGRHDFSAFRAAE
CQARSPVKELRRALIERRGDYLLCDFRADGFLHHMVRNLMGCLVQIGAGGRPPEWLHEVLAGRDRTRAAPTFEAAGLYLT
HIRYPARFALPESSERWPFA

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=288, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788657, Length=252, Percent_Identity=46.4285714285714, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_THIDA (Q3SHL7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315674.1
- ProteinModelPortal:   Q3SHL7
- SMR:   Q3SHL7
- STRING:   Q3SHL7
- GeneID:   3674026
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1916
- NMPDR:   fig|292415.3.peg.2013
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q3SHL7
- ProtClustDB:   PRK00021
- BioCyc:   TDEN292415:TBD_1916-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28713; Mature: 28713

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIAVGLEYRGVGFCGWQSQPQACGVQDAVEKAVSAIAGEAITVTAAGRTDTGVHAALQ
CEEEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHEEE
IVHFDTTSVRPLTAWVRGVNSHLPAGVAVLWAREVDAEFHARFAAFERGYRYVLLNHPVR
EEEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHCCCEEEEEECCCC
PGLNAGLVGWHHRPLDVACMNRAASPLIGRHDFSAFRAAECQARSPVKELRRALIERRGD
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCC
YLLCDFRADGFLHHMVRNLMGCLVQIGAGGRPPEWLHEVLAGRDRTRAAPTFEAAGLYLT
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCEEEE
HIRYPARFALPESSERWPFA
EECCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MMRIAVGLEYRGVGFCGWQSQPQACGVQDAVEKAVSAIAGEAITVTAAGRTDTGVHAALQ
CEEEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHEEE
IVHFDTTSVRPLTAWVRGVNSHLPAGVAVLWAREVDAEFHARFAAFERGYRYVLLNHPVR
EEEECCCCCHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHCCCEEEEEECCCC
PGLNAGLVGWHHRPLDVACMNRAASPLIGRHDFSAFRAAECQARSPVKELRRALIERRGD
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCC
YLLCDFRADGFLHHMVRNLMGCLVQIGAGGRPPEWLHEVLAGRDRTRAAPTFEAAGLYLT
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCEEEE
HIRYPARFALPESSERWPFA
EECCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA