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The map label for this gene is mlaE [H]

Identifier: 74317917

GI number: 74317917

Start: 1996116

End: 1996904

Strand: Reverse

Name: mlaE [H]

Synonym: Tbd_1899

Alternate gene names: 74317917

Gene position: 1996904-1996116 (Counterclockwise)

Preceding gene: 74317918

Following gene: 74317916

Centisome position: 68.63

GC content: 66.29

Gene sequence:

>789_bases
ATGCTTAGGCAGGCCATCGAAAACCTCGGCGCCCGCACGATAGACGCGGTCTGGCGCATCGGCTTCGCGACCCGTTTCTT
CGCCGCGACGCTGCTGCATTCGGGCACCGCGTTCCGCCGTTTCGGCCTGACCGTGCGTGAGCTCTATTTCACCGGCGTGC
TGTCGCTCATCATCATCCTGGTGTCGGGCCTCTTCGTCGGCATGGTGCTCGGGCTGCAAGGCTACGAGACCTTGCAGACC
TACGGCTCGGAGGAAGCGCTCGGCATCCTGGTCGCGCTGTCGCTGGTGCGCGAACTCGGCCCGGTCGTCGCGGCCTTGCT
GTTCGCCAGCCGCGCCGGCAGCGCGATCACGGCCGAGATCGGCCTCATGAAGGCGACCGAACAGCTTTCGGCGATGGAAA
TGATGGCCGTCGACCCGATCGCCCGCGTCGTCGCGCCGCGCTTCTGGGCAGGCGTGATCTCGATGCCGCTCCTGGCGGCA
CTGTTCTCGGCCATGGGGGTGTTCGGCGGCTACCTCGTCGGCGTGCAGCTGATCGGGGTCGACGAGGGCGCGTTCTGGTC
GCAGATGCAAAGTGCGGTGGATTTCGAGCAGGACATCCTGAACGGCGTCATCAAGAGCGTCGTCTTCGGCGTCGCGATCA
CCGCGATCGCGCTGTTCGAGGGCTACGACGCGCCGCCGACCGCCGAGGGGGTGTCGCGGGCGACGACCCGTACCGTGGTG
ACCTCGAGCCTCGCGGTGCTCGCGCTCGATTTCGTTTTGACTGCATTCATGTTCCGGGGAAGCAACTGA

Upstream 100 bases:

>100_bases
TCCTACGTGCACCAGTTCGTCCACGGCGAAGCGGACGGCCCGGTACCCTTCCACTACCCGGGGCCGGACTACGCCACCGA
CCTCCAGTTGGGAGCCATGC

Downstream 100 bases:

>100_bases
CAATGAACAAGACCGTACTCGATCTCTGGGTGGGATTATTCGTCATCGCGGGAATCGTGGCCCTGCTGTTCCTCGCGCTC
AAGGTCGGCAGCATGAGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLRQAIENLGARTIDAVWRIGFATRFFAATLLHSGTAFRRFGLTVRELYFTGVLSLIIILVSGLFVGMVLGLQGYETLQT
YGSEEALGILVALSLVRELGPVVAALLFASRAGSAITAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMPLLAA
LFSAMGVFGGYLVGVQLIGVDEGAFWSQMQSAVDFEQDILNGVIKSVVFGVAITAIALFEGYDAPPTAEGVSRATTRTVV
TSSLAVLALDFVLTAFMFRGSN

Sequences:

>Translated_262_residues
MLRQAIENLGARTIDAVWRIGFATRFFAATLLHSGTAFRRFGLTVRELYFTGVLSLIIILVSGLFVGMVLGLQGYETLQT
YGSEEALGILVALSLVRELGPVVAALLFASRAGSAITAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMPLLAA
LFSAMGVFGGYLVGVQLIGVDEGAFWSQMQSAVDFEQDILNGVIKSVVFGVAITAIALFEGYDAPPTAEGVSRATTRTVV
TSSLAVLALDFVLTAFMFRGSN
>Mature_262_residues
MLRQAIENLGARTIDAVWRIGFATRFFAATLLHSGTAFRRFGLTVRELYFTGVLSLIIILVSGLFVGMVLGLQGYETLQT
YGSEEALGILVALSLVRELGPVVAALLFASRAGSAITAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMPLLAA
LFSAMGVFGGYLVGVQLIGVDEGAFWSQMQSAVDFEQDILNGVIKSVVFGVAITAIALFEGYDAPPTAEGVSRATTRTVV
TSSLAVLALDFVLTAFMFRGSN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=258, Percent_Identity=62.4031007751938, Blast_Score=310, Evalue=6e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27859; Mature: 27859

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQAIENLGARTIDAVWRIGFATRFFAATLLHSGTAFRRFGLTVRELYFTGVLSLIIIL
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSGLFVGMVLGLQGYETLQTYGSEEALGILVALSLVRELGPVVAALLFASRAGSAITAEI
HHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMPLLAALFSAMGVFGGYLVGVQLIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DEGAFWSQMQSAVDFEQDILNGVIKSVVFGVAITAIALFEGYDAPPTAEGVSRATTRTVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TSSLAVLALDFVLTAFMFRGSN
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRQAIENLGARTIDAVWRIGFATRFFAATLLHSGTAFRRFGLTVRELYFTGVLSLIIIL
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
VSGLFVGMVLGLQGYETLQTYGSEEALGILVALSLVRELGPVVAALLFASRAGSAITAEI
HHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLMKATEQLSAMEMMAVDPIARVVAPRFWAGVISMPLLAALFSAMGVFGGYLVGVQLIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DEGAFWSQMQSAVDFEQDILNGVIKSVVFGVAITAIALFEGYDAPPTAEGVSRATTRTVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TSSLAVLALDFVLTAFMFRGSN
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]